LOCUS       c00100_gnlPro..         6114 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|CNMKKNMO_100.
ACCESSION   c00100_gnlPro..
VERSION     c00100_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 03:32:07
            Original ID  :: gnl|Prokka|CNMKKNMO_100
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6114
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..687
                     /locus_tag="ctg100_1"
                     /transl_table=11
                     /translation="ETLKALEPYGPMMFSGLDTHPGARKYKFRVKAPVLRAAPPAHRVQ
                     LYLQLGVLHGLDVATPSLFPVKKAAPAEDAPILIAPFSRLGSASEWSREQWRELVSLLP
                     GRTVLAALEEDRERAASLAEHLNVELAVGAPEALFPVMDGAAAAVAVDGDFPSLCSFRG
                     LPVVTLFSTRLPDVYRPMGTFNRSLYSHQCCSPCFRKECDRDVPCNRHIAVRDVLEALR
                     EISGKE"
     protocluster    1..6114
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[935:3999]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      936..3999
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..6114
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6114
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(936..1715)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 54.5;
                     E-value: 4.9e-17)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg100_2"
                     /sec_met_domain="Radical_SAM (E-value: 7e-32, bitscore:
                     99.5, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQVFPQNHDPDGESSVTGLVHSVESCGTVDGPGIRFVLFLSGCS
                     LRCRYCHNPDTSYVRRGQLRSADDVLKEIARYRDFLQAAGGGVTLSGGDPLFQPDFAGA
                     VLKGCRKLGLHTCLDTSGHLGVNAGEEMLADTDLVLLDIKAWNPKRYRALTGGELRPTL
                     EFAERLASLRKPVWLRYVLVPGVTDNLEEIEELSRHAALLGNVERVDVLPFHQLGRFKW
                     DELKLDYTLKDVPEASAELAEQVRGIFRRGGFPDCVH"
     CDS             complement(1732..3999)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg100_3"
                     /sec_met_domain="Gly_radical (E-value: 2.2e-46, bitscore:
                     145.3, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 9.1e-282, bitscore:
                     924.1, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MISIVKDLKESTALPQEWQGFKPGAWTESIDVRDFIQHNYTPYSG
                     NEEFLSGPSQRTLRLWDELKVLLKREIDNGGVLDADEEVVSSITSHKPGYIDKELEVVV
                     GLQTDAPLKRALMPFGGLRMAQQALESYGFKMCEKTADIFKKIRKTHNEGVFDAYTSDI
                     RAARSAGIITGLPDAYGRGRIIGDYRRVALYGTDKLIAERRKDLKNREHSPLTDELIRL
                     REEMSEQIRALEELAQLGASYGCDLTRPAANAREAVQWTYLGYLAAVKEQNGAAMSLGR
                     VSTFFDIYFTRDLEQRLITEEEVQEIIDQFVMKLRIVRFIRTPDYNNLFSGDPTWVTES
                     IGGMGEDGRTLVTRSSFRMLQTLYNLGPAPEPNLTVLWSRNLPEAFKSFCAKVSIETSS
                     VQYENDDLMRPHWGDDYGIACCVSAMRIGKQMQFFGARANLAKCLLYALNGGVDELKGK
                     QVAPPSPRYTEEILNYDEVMALYDKMQDWLAKTYIDALNIIHYMHDKYCYERIEMALHD
                     PEILRTMATGIAGLSVAADSLSAIKYATVKAIRNEEGLIVDFKTEGEFPCYGNNDPRVD
                     DIACSLVSNFMEKLRRLHTYRNSLPTQSILTITSNVVYGKKTGNTPDGRRAGEPFAPGA
                     NPMHGRDRNGAVASMLSVAKLSYDDSLDGISYTFSIVPQALGKEERERRVKLVSLLDAY
                     FAATGHHINVNVLERETLLDAMDHPEKYPQLTIRVSGYAVNFIKLTREQQQEVINRTFH
                     TR"
     CDS             4280..5062
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lipase_GDSL_2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg100_4"
                     /sec_met_domain="Lipase_GDSL_2 (E-value: 2.5e-24, bitscore:
                     75.1, seeds: 1564, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSYACLCLLLAGVFCVACFSGACADELPPTLKPQVNPRAVYDWK
                     KRHEAVMERNRTVKPEYVVFGDSITHRWGGEPSGDNRALGTGKTAWDSLFSSHAVTNMG
                     FGSDYVDNAYYRLQLGELDGISPRVIIVLLGTNNLGGRKDAPRVCADNLKAFVSLARRK
                     CPSSKILLLGILPREEKKLAPLIRETNKMISGLADGNSVFFANPGEEFLGEDGVSPKPG
                     LLEDGLHPSARGYDILGKSLSVLLKKMDDKYGKLPAPR"
     CDS             5285..6073
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lipase_GDSL_2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg100_5"
                     /sec_met_domain="Lipase_GDSL_2 (E-value: 3.3e-24, bitscore:
                     74.7, seeds: 1564, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNFLSYILAILLGSSSLAFSAPSIPASALPATLKPEPHQRDPYDW
                     NARHEAVKKRNRAVKPEYVMIGDSITHHWGGEPSGDGKNTGSGSWEKLFGPHAATNMGF
                     GFDYVDNAYYRVQNGELDGISPRVIIVLLGTNNLGHRKDTPQACADNIRAFVRLVRRKC
                     PSSKILLLGVLPRKEKNLAEPVKQTNRLLAELHNGKDIFFADPGKALLSADGASPQNEF
                     MKDVVHPNARGYEVLEKELAVLLKKLDAKYRGGKQAGKHS"
ORIGIN
        1 gaaaccctga aagcgctgga accgtatggc cccatgatgt tttccgggct ggacacgcat
       61 cccggtgccc ggaagtacaa gttccgggta aaggcccccg tcctgcgcgc cgcccccccg
      121 gcgcaccggg tgcagcttta tctgcaattg ggggtgctgc atgggctgga cgtcgccacc
      181 ccgtctttgt tccctgtgaa aaaagcggcc ccggcggaag acgcccccat tttaatcgct
      241 ccgttttccc gcctggggag cgccagtgaa tggagcaggg agcagtggag ggagctggtt
      301 tcccttctgc cgggacggac ggtgctggcg gctctggaag aagaccggga gcgtgccgcc
      361 tccctggcgg aacatttgaa tgtggagctg gcggtgggtg cgccggaggc tctctttccc
      421 gtcatggacg gcgctgcggc ggcagtagcc gtggatggcg actttccgtc cctctgttct
      481 ttccgggggc ttccggtggt gactctgttt tccacccggc ttccggatgt ttaccgccct
      541 atgggcacat tcaaccgttc cctttacagc caccagtgct gttctccctg cttccggaag
      601 gaatgtgacc gtgatgttcc ctgcaaccgc cacattgccg tgcgggacgt actggaagcc
      661 ttgcgggaaa tttccgggaa agaataatct gggaagggac ggaatgccgt cccccggcgt
      721 tggcggaaaa aacggcggga atgctgccgt tgccgtaaag aatgatgaaa taacgaaagg
      781 aattgcggaa atgccgtatt ccggagcaat tccagaaatg cgtcttcctt tttgcatgga
      841 ttctgcagtg gaggggagag ggcccctcat ccggaagagg gggatgcctg cgtccggttt
      901 gctgcggttg gtggcgccaa agcgcgaaaa tccggttaat gcacgcaatc cggaaagcct
      961 ccccgccgga aaatgccgcg gacctgttcc gccagttctg cggacgcttc cgggacgtcc
     1021 ttcaaagtat agtccagctt caattcatcc catttgaatc tccccagctg gtggaaaggg
     1081 agcacgtcca cgcgttccac gttgcccaga agcgccgcat ggcgggagag ttcctcaatt
     1141 tcttccagat tgtccgtaac gccaggtacc agcacatacc tcagccacac gggcttgcgg
     1201 agggaggcca ggcgttccgc aaactccagt gtggggcgca gttctccgcc tgtcagggcg
     1261 cgataccgtt tcggattcca tgccttgata tccagcagga ccaggtccgt atccgccagc
     1321 atttcttcgc ccgcattcac gcccagatgg ccggaggtgt ccaggcaggt gtgcaggccc
     1381 agtttccggc agcctttcag cacggcaccg gcaaaatccg gctggaagag gggatccccg
     1441 ccggagaggg tgactccgcc gcccgccgcc tgcaaaaaat cccggtaacg ggctatttcc
     1501 ttgaggacat catctgcgct cctgagctgg cccctccgca cgtaggaggt atccggatta
     1561 tggcagtagc ggcaacgcag gctgcagccg gacaggaaaa ggacgaagcg gatgccgggg
     1621 ccgtccacgg tgccgcagga ttccacggaa tgaaccaaac cggtgacgga gctttctccg
     1681 tccggatcat ggttttgtgg aaaaacctgc ggcatccgct tgcagtccgg gttaacgggt
     1741 gtggaaagtg cggttgatga cctcctgctg ctgttcccgg gtcagcttga tgaaattcac
     1801 ggcatagccg gaaacgcgga tggtaagctg cgggtatttt tccgggtgat ccatggcgtc
     1861 gagaagcgtt tcccgttcca gtacgttcac attaatgtgg tgacctgtag cggcaaagta
     1921 ggcgtccagc agggagacga gcttgacgcg gcgttcacgt tcctccttgc ccaaggcctg
     1981 aggaacgatg gagaaggtgt aggagatgcc gtccagggag tcgtcatagg acagcttggc
     2041 tacggagagc atggaggcca cggctccgtt cctgtccctt ccgtgcatgg ggttggctcc
     2101 gggggcgaac ggttccccgg ctcggcggcc gtccggcgtg ttgcccgtct ttttgccgta
     2161 caccacgttg gaggtgatcg tcaggatgga ctgggtgggc agggaattgc ggtaagtgtg
     2221 gagcctgcgc agtttttcca tgaaattgct gaccaggctg catgctatgt cgtccacgcg
     2281 cgggtcattg ttcccgtaac aggggaattc cccttccgtc ttgaagtcca caatcagccc
     2341 ttcttcattg cggatggctt ttacggtggc atacttgatg gcggacaggg agtccgccgc
     2401 cacggaaagc ccggcgattc ccgtagccat cgtgcgcaga atttccggat catgcagcgc
     2461 catttcaatg cgttcatagc agtatttgtc gtgcatgtag tggatgatgt tcagcgcgtc
     2521 aatgtaagtt ttggcgagcc agtcctgcat cttgtcgtac agggccatca cctcatcata
     2581 gttgagaatt tcctccgtgt agcgggggga gggcggggcc acctgcttgc ccttgagttc
     2641 atccacgccg ccgttcaggg cgtagagaag gcacttggcg aggttggcgc gcgctccgaa
     2701 gaactgcatc tgcttgccga tgcgcatggc ggacacgcag catgcgatgc cgtagtcgtc
     2761 gccccaatgc gggcgcatca ggtcgtcgtt ctcgtattgg acggaggacg tttcaatgga
     2821 aaccttggcg cagaagcttt tgaaggcttc cggcaggttc cgggaccaca gaacggtcag
     2881 gttcggttcc ggagccggcc ccaggttgta cagggtctgc agcatgcgga aggaactgcg
     2941 ggtgacgagg gtgcgcccgt cctctcccat gccgccgatg gattccgtca cccaggtggg
     3001 gtctccggag aacaggttat tgtaatcagg cgtgcggatg aaacggacga tgcgcagctt
     3061 catcacgaac tggtcgatga tttcctgaac ttcctcttcc gtgatgagcc tctgttccag
     3121 gtcgcgggtg aaataaatgt cgaagaaagt ggaaacgcgg cccagggaca tggccgctcc
     3181 gttctgttcc ttcacggcgg ccaggtagcc caggtacgtc cattgcacgg cttcgcgggc
     3241 gtttgcggcg gggcgagtga ggtcgcagcc gtaggaggcg cccagctggg cgagttcttc
     3301 cagggcgcgt atttgttcgc tcatttcttc gcgcagacgg atcagctcat ccgtaagggg
     3361 ggaatgttca cggtttttga ggtccttgcg gcgttcggca atcagcttat ccgtgccgta
     3421 cagggccacc cggcggtagt ccccgatgat gcggccgcgg ccataggcgt ccggaaggcc
     3481 ggtaataatc ccggcggaac gggccgcccg gatatcggaa gtgtaggcgt cgaaaacgcc
     3541 ttcattgtgc gtcttgcgta tctttttgaa aatgtctgcg gttttctcgc acatcttgaa
     3601 cccgtaggat tccagggcct gttgggccat gcgcaagccg ccaaagggca tcagggcccg
     3661 tttcaggggg gcgtccgtct ggaggcctac gaccacttcc agttccttgt caatgtagcc
     3721 gggcttgtgg gatgtgatgg aggataccac ttcttcatca gcatccagca cgccgccgtt
     3781 gtctatttcc cgtttcagca ggactttcag ctcgtcccat aaacgaagcg tgcgctggga
     3841 ggggccggaa agaaactctt cattgccgga atagggcgtg tagttatgct ggatgaaatc
     3901 ccgtacgtca atggactccg tccatgctcc cggcttgaat ccctgccatt cctgcggaag
     3961 ggcggtggac tccttcaagt cttttactat gctgatcata aatagaatgt agtgaagaag
     4021 actggagaaa aacgttccgc agcccctgaa aggctggaaa cggcaaaact ccgcgccgcg
     4081 taattatatc gtgaattccg ggggacaaca aggctaattc tttgcgaaga gaacgcgggt
     4141 gcgctggctt tggcatgttt cacctgtctg tccgttgcgg agcggaaaat gaggggaaca
     4201 tgcgattgcg gaaaagtgac ggaagattat ttctctttac tttcctaatc tctattgcag
     4261 tatctcaacg cttaccgtta tgaaatctta tgcatgcctt tgtttattgc tggcgggagt
     4321 tttttgcgtt gcttgtttct ccggggcctg tgctgatgaa ttgccgccca cgctgaagcc
     4381 ccaggtgaac ccgcgggccg tgtatgactg gaaaaaacgt catgaagcag tgatggagcg
     4441 gaaccggacc gtcaaaccgg aatatgtcgt ctttggtgac agcattaccc accgctgggg
     4501 cggcgagcct tccggggaca acagggctct cggaacagga aaaaccgcct gggatagcct
     4561 gttttcttcc catgcggtaa ccaatatggg gtttggttcc gattacgtgg ataatgccta
     4621 ttaccgcttg cagctgggag agctggacgg catttctccg cgggtcatca tcgtgctgct
     4681 gggaaccaat aatcttggcg ggcggaagga tgctccccgg gtctgcgcag acaatctcaa
     4741 ggcttttgtg agcctggcgc gccggaagtg cccctcttcc aaaatcctgt tgctgggcat
     4801 tctccccagg gaggaaaaaa agctggctcc cctgattagg gaaaccaata aaatgatatc
     4861 cggcctggct gacggaaaca gcgtgttttt cgccaatccc ggcgaggaat tcctggggga
     4921 ggacggagtg tccccaaaac ccgggctact ggaggatggg ctgcatccca gtgccagggg
     4981 gtacgacata ctggggaagt cgttgagcgt attgctgaag aaaatggacg acaagtacgg
     5041 caagctgccc gctccccggt agatgaatac gggggagggc gttcctgttc cgggagaggc
     5101 cgggggatgg atgaaatgga gcgcgccttg gaagaggccg aacacggtgc tcgcctggcc
     5161 gtggttgcgg caggatttgc cggacgggca gtggtctttt ttactctgcc ggcggaagtg
     5221 acgcctgcga tcttgatgca cggcaggatt tgcttaaact gaaaaccggt tttacttttt
     5281 gattatgaat ttcctctcct acatactcgc catattgctg gggagttcca gtctggcatt
     5341 ttctgcgcct tccattccgg cctccgcgtt gcccgctacc ctgaagccgg agccccatca
     5401 gcgggacccg tatgattgga atgcccggca tgaagccgtt aaaaaaagaa accgggccgt
     5461 caagcctgaa tacgtgatga taggggacag cattacccac cattggggag gggagccttc
     5521 cggggacgga aaaaatacgg gatcgggttc ctgggagaaa ttattcggtc cgcatgcagc
     5581 gacgaatatg ggctttggat ttgattatgt ggataatgcc tattaccgtg tccaaaacgg
     5641 ggagctggac ggcatttctc cgcgggtcat cattgtgttg ctggggacca ataatctggg
     5701 gcaccgcaag gatacgccgc aggcatgtgc ggacaatatc agggcatttg tccgcctggt
     5761 gcgccggaag tgcccctctt ccaaaatcct gttgctgggc gttctcccac ggaaggagaa
     5821 aaacctggcg gaaccggtga agcagacgaa cagactgttg gcggaactgc ataacggaaa
     5881 ggacattttc tttgccgatc cgggaaaggc gcttctttcc gcagatggtg cttctccgca
     5941 gaatgaattc atgaaggatg tggtgcatcc caatgccagg gggtacgagg ttttggaaaa
     6001 ggaactggcc gtactcctga aaaagcttga tgcaaaatac cggggaggga agcaggcggg
     6061 aaagcattcc tgatgatcgt tttctgatgg aaggtaggat tttgcttgaa ggga
//
