LOCUS       c00082_gnlPro..         4350 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|CCNFOFOP_82.
ACCESSION   c00082_gnlPro..
VERSION     c00082_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 04:10:51
            Original ID  :: gnl|Prokka|CCNFOFOP_82
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4350
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4350
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[315:4350]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      316..4350
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..4350
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4350
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             2..187
                     /locus_tag="ctg82_1"
                     /transl_table=11
                     /translation="IMDRGFFRHLKNIWLGLDVFNILDIKNVSSYYWITDVYNVQYAVP
                     NYLTGRQLNVRLIVDF"
     CDS             316..726
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Glyoxalase_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg82_2"
                     /sec_met_domain="Glyoxalase_4 (E-value: 1.3e-27, bitscore:
                     85.0, seeds: 51, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELTHIEHLGIAVKSIEACLPYYEGVLGLKCYNIEVVADQKVKTA
                     FFKVGQTKIELLEPTSEDSTIAKFIEKKGEGIHHIAFAVPDVQAALNEAEEKGVKLIDK
                     APRGGAEGLNIAFLHPKSTCSVLTELCMPGNK"
     CDS             763..2316
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Btheta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg82_3"
                     /sec_met_domain="CT_Btheta (E-value: 1.2e-292, bitscore:
                     959.2, seeds: 72, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNQLEKVKELIALREQARLGGGEKRIESQHKKGKYTARERIAML
                     LDEGSFEEFDMFVQHRCTNFGIEKTKYLGDGIVTGYGTIDGRLVYIFAQDFTVFGGALS
                     EALAMKICKVMDQAMKMGAPVIGLNDSGGARIQEGVNALAGYAEIFQRNILASGVVPQI
                     SGIFGPCAGGAVYSPALTDFNIMTRGTSYMFLTGPKVVKTVTGEDVTQEQLGGASVHTT
                     KSGVAHFAVDTEEDGLLLIRKLLSFLPQNNLEETPLVECKDPIDRLDDVLNDIIPDNAN
                     QSYDMYEVIGTIVDNNEFLEVHADYAKNIIVGFARFNGQSVGIVANQPKVMAGCLDSNA
                     SRKAGRFVRFCDAFNIPLVTLVDVPGFLPGTGQEYNGVILHGAKLLYAYGEATVPKITV
                     TLRKSYGGAYCVMSSKHLRGDMNYAWPTAEIAVMGPSGAVEVIFAKEVAAAEDPKACAA
                     EKEAEYKKAFANPYNAAQYGYIDDVIEPRNTRFRIIRALQQLQTKKLSNPAKKHDNLPL
                     "
     CDS             2338..3231
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg82_4"
                     /sec_met_domain="OAD_gamma (E-value: 8e-14, bitscore: 40.9,
                     seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINKKIGVLLLLMVLFNMGAKAQLTTSVKLNEVLVVNEDNFVDDY
                     GKRHPWFELFNNSAGTVDLRGCFLTNDKSNPRKYMIPKGDVLTKVPPRQHLLFWADDQP
                     TKGTFHVNFTFDPNKDNYLALYDSDGTTLIDEVTIPAGQKADVSYGLDLDGVGNWSILD
                     KVTPSTNNLTLDSNAKLENFQRNDPWGIGMTITAMAVVFLGLIILYLVFKQIGNAAIKA
                     SKRNAQKAAGTGAAVSANIGQESGEIFAAIATALYEMSDDNHDIENTVLTIHKVQRSYS
                     PWSSKLYSLRQTPNKK"
     CDS             3255..3686
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 63.7; E-value:
                     3.5e-20)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg82_5"
                     /sec_met_domain="Biotin_lipoyl (E-value: 3.4e-21, bitscore:
                     63.7, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSFKYTINGNTYKVHINSVVDDVAEVEVNGTPYNVKMEKPAKKQ
                     MVTLKRPAQAPTNASGEPVITRQAAPSVAGAVKSPLPGVILSVDCKVGDTVKRGQKILV
                     LEAMKMENNINADRDGKIVEIKVNKGDSVLEGADLVVIG"
     CDS             3691..4350
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg82_6"
                     /sec_met_domain="OAD_beta (E-value: 1.5e-83, bitscore:
                     269.3, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQESFLQFLGENIQIFLSYTGFANATPGHIVMLLVGLLFIFLAIK
                     YEFEPMLLIPIGFGILIGNIPFKDAGLQLGIYEEGSVLNILYQGVKQGWYPPLIFLGIG
                     AMTDFSALISNPKLMLVGAAAQFGIFGAYIIALQMGFDPSQAGAIGIIGGADGPTAIFL
                     SSKLAPNLMGAIAVSAYSYMALVPIIQPPFMRLLTTKKERVIRMKPPRAVSQTEKIV"
ORIGIN
        1 tatcatggac aggggcttct tccgccattt gaaaaacatt tggctaggtc tggatgtatt
       61 taacatatta gacatcaaaa acgttagttc ctactattgg ataacagatg tatataatgt
      121 ccaatacgca gttccaaact atctgaccgg acgtcaattg aacgttcgtc tgattgtcga
      181 tttttaataa aatgtatctt taaacacatt tactgggaga taaaaacaaa aaattatttc
      241 ctcattcata aaaagtagtg tacatttgca cacaatagaa acaaacattt actaaatctt
      301 taaattatta ttgacatgga acttacacat atcgaacatt taggtatcgc agtgaagagt
      361 atcgaggctt gtttacccta ttatgaaggc gttcttggat taaaatgcta caatatcgaa
      421 gtagtagcag accaaaaagt aaaaaccgca tttttcaagg taggacagac taagattgaa
      481 ttactagagc cgaccagcga agatagcacg atcgcaaaat ttatcgagaa aaaaggtgaa
      541 ggtatccacc atatcgcatt cgcggtacct gatgtacagg cagctttgaa cgaggctgag
      601 gaaaaaggcg ttaaattaat cgataaagct ccccgtggtg gcgcggaagg cttgaacatc
      661 gctttccttc acccgaaatc aacttgcagc gtattgacag agctttgcat gccgggcaac
      721 aaataattaa tccgttcatt tctaacaaca aatcaaaata caatgagtaa ccagcttgaa
      781 aaagtaaaag agctcatcgc acttcgtgag caagctcgct tgggtggagg agagaaaaga
      841 atcgaatcac agcacaaaaa aggcaaatat accgctcgtg agcgtatcgc catgttgctt
      901 gacgaaggta gtttcgagga attcgacatg tttgttcaac accgttgtac aaacttcggt
      961 atcgagaaaa ctaaatattt aggcgatggt atcgtaacag gttatggtac gatcgacgga
     1021 cgtttggtat atatcttcgc tcaagatttc accgtattcg gtggagcctt gtctgaggct
     1081 ttggctatga agatctgtaa agtaatggat caagccatga agatgggcgc tcccgttatc
     1141 ggtttgaacg actccggtgg agctcgtatc caagaaggtg taaacgcctt ggcaggatac
     1201 gcagagattt tccaacgtaa catcttggct tccggtgttg ttccccagat ttccggtatc
     1261 ttcggccctt gcgccggtgg agccgtttat tctccggctt tgacagactt caacatcatg
     1321 acacgtggta ccagctatat gttcttgact ggtccgaaag tcgtaaaaac cgtaacaggt
     1381 gaggatgtaa cccaagagca gttgggtgga gccagcgtac acactaccaa atcgggtgta
     1441 gcccatttcg ctgtcgatac agaagaagat ggtttgctat tgatccgtaa attgctcagt
     1501 ttcttacctc agaacaactt agaggaaacg ccgttagttg agtgcaaaga cccgatcgat
     1561 cgtctggatg atgtattgaa cgatatcatt cccgataacg ccaaccaatc ttacgatatg
     1621 tatgaggtta tcggtacgat cgtcgataac aacgagttcc tagaggtcca tgcggactac
     1681 gctaagaata tcatcgtagg tttcgctcgt ttcaacggac aatccgtcgg tatcgtagct
     1741 aaccagccga aggttatggc aggatgcttg gatagcaacg catctcgtaa agcaggtcgc
     1801 ttcgttcgtt tctgcgacgc tttcaatatt cctttggtaa cattggttga cgttccggga
     1861 ttccttccgg gtacaggtca ggaatacaac ggtgtaatcc ttcatggcgc taaattgctt
     1921 tacgcttatg gtgaggctac cgttcctaag attaccgtta cattgcgtaa gtcttacggt
     1981 ggagcttatt gcgtgatgag ttctaaacat ttacgtggtg atatgaatta cgcatggccg
     2041 acagccgaga tcgccgttat gggcccgagc ggtgccgtag aggttatctt cgccaaggaa
     2101 gtagcagccg ctgaagatcc gaaagcatgc gcagcagaga aagaagctga atataagaaa
     2161 gcattcgcta atccttataa cgcagctcaa tacggttata tcgacgatgt tatcgaaccg
     2221 cgtaacactc gtttccgtat tattcgtgct ttgcagcaat tgcagactaa gaagttaagc
     2281 aatcctgcta agaaacatga taacctgcct ctataatccg taaaacagga gatgaatatg
     2341 ataaataaga aaataggggt attgctactg ttaatggttc tcttcaatat gggagccaag
     2401 gcacagttga ctacctccgt taaattaaat gaagtgctag ttgtcaatga agataacttt
     2461 gttgatgact atggaaaacg ccatccatgg tttgagttat ttaataactc agcaggtacg
     2521 gtggatttac gaggatgctt cttaacgaac gataaaagta atcccagaaa atatatgatc
     2581 ccgaagggag atgtacttac caaagttcct ccccgccagc atcttctttt ttgggcagat
     2641 gatcagccaa caaaagggac atttcatgta aacttcacat tcgatccaaa caaagataat
     2701 tatctagcct tatatgattc agatggtaca actttgatcg acgaggtgac aatccctgcc
     2761 gggcaaaaag cagatgttag ttacggtctg gatcttgatg gagtaggaaa ttggagtatc
     2821 cttgacaaag tgactcctag caccaataat ctgacgttag attcgaatgc gaaacttgag
     2881 aacttccaac ggaacgaccc atggggtatt ggtatgacca taacagccat ggcagtcgta
     2941 ttccttggct taatcatttt gtatctagta ttcaagcaga tcggtaacgc agcgatcaaa
     3001 gctagcaagc gtaacgctca aaaagccgca ggaacaggtg ctgccgtatc agccaatatc
     3061 ggtcaagaat caggtgaaat ttttgccgct atcgctaccg ctctttatga gatgagcgat
     3121 gacaaccacg atatcgagaa cacggtattg actatccaca aggttcaacg cagctattca
     3181 ccatggagtt ctaagcttta ttctctgcgt cagactccaa ataaaaagta atcatcctaa
     3241 aatccaattg acgaatgaaa agttttaaat ataccattaa cggtaacaca tataaagtac
     3301 atatcaactc agttgtagat gatgtggcag aggtagaggt aaacggaacc ccttataacg
     3361 taaaaatgga gaaacctgcg aaaaaacaaa tggttacatt gaaacgtccg gctcaagctc
     3421 cgaccaatgc cagcggtgaa cctgttatca cccgtcaagc agctccaagt gtagccggcg
     3481 ctgttaaatc tccgctgcca ggtgtaattt taagtgtaga ctgtaaagtc ggcgatacag
     3541 ttaagagagg ccaaaagatc ttggtattgg aagccatgaa gatggaaaac aacatcaatg
     3601 ccgatcgtga cggtaaaatt gtagaaatta aagtaaacaa aggcgactct gtattggaag
     3661 gtgcagatct cgttgtaatc ggttaatgat atgcaagaaa gtttcttaca attcctagga
     3721 gaaaatatcc aaatatttct ctcctataca ggatttgcga acgcgactcc agggcatatc
     3781 gtgatgctat tggtaggttt gctgttcata ttcctagcga ttaaatatga attcgagcca
     3841 atgcttctga tccctattgg tttcggtatc ttgatcggaa atatcccttt caaggatgcc
     3901 ggattacaat taggtatata cgaagagggt tctgtattga acattttata ccaaggagtg
     3961 aaacaaggtt ggtatccccc cttgatattc ctcggtatcg gtgccatgac agacttctcc
     4021 gctttgatat ccaatcctaa gttaatgcta gtcggagctg ccgcccaatt tggtattttc
     4081 ggtgcttata tcattgcatt gcaaatggga tttgacccga gccaagccgg tgctatcggt
     4141 attatcggag gagctgatgg tccaaccgca atcttcttgt catccaagtt agctcctaac
     4201 ttgatgggtg ctatcgctgt atccgcatac tcatatatgg cattggttcc tattatccag
     4261 ccaccgttta tgagattgct tacaacgaag aaagaacgtg tgatacgtat gaaacctcca
     4321 agagccgttt cacagacgga gaaaatcgtt
//
