LOCUS       c00181_gnlPro..         4012 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PFFMJECB_181.
ACCESSION   c00181_gnlPro..
VERSION     c00181_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 06:28:36
            Original ID  :: gnl|Prokka|PFFMJECB_181
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4012
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4012
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:2759]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2..2759
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4012
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4012
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..1963
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg181_1"
                     /sec_met_domain="Gly_radical (E-value: 1.1e-46, bitscore:
                     145.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 4.6e-249, bitscore:
                     815.4, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="SDLPLKRALMPFGGLRMAQQAMESYGFKMCEKTADIFKKIRKTHN
                     EGVFDAYTSDIRAARSAGIITGLPDAYGRGRIIGDYRRVALYGTDKLIAERRKDLKNRE
                     HSPLTDELIRLREEMSEQIRALEELAQLGASYGCDLTRPAANAREAVQWTYLGYLAAVK
                     EQNGAAMSLGRVSTFFDIYFTRDLEQGLITEEEVQEIIDQFVMKLRIVRFIRTPDYNNL
                     FSGDPTWVTESIGGMGEDGRTLVTRSSFRMLQTLYNLGPAPEPNLTVLWSRNLPEAFKS
                     FCAKVSIETSSVQYENDDLMRPHWGDDYGIACCVSAMRIGKQMQFFGARANLAKCLLYA
                     LNGGVDELKGKQVAPPSPRYTEEILNYDEVMALYDKMQDWLAKTYIDALNIIHYMHDKY
                     CYERIEMALHDPEILRTMATGIAGLSVAADSLSAIKYATVKAIRXXXXRNEEGLIVDFK
                     TEGEFPCYGNNDPRVDDIACSLVSNFMEKLRRLHTYRNSLPTQSILTITSNVVYGKKTG
                     NTPDGRRAGEPFAPGANPMHGRDRNGAVASMLSVAKLSYDDSLDGISYTFSIVPQALGK
                     EERERRVKLVSLLDAYFAATGHHINVNVLERETLLDAMDHPEKYPQLTIRVSGYAVNFI
                     KLTREQQQEVINRTFHTR"
     CDS             1980..2759
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 53.8;
                     E-value: 8.4e-17)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg181_2"
                     /sec_met_domain="Radical_SAM (E-value: 4.8e-33, bitscore:
                     102.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQVFPQNRDPAGESSVTGLVHSVESCGTVDGPGIRFVLFLSGCS
                     LRCRYCHNPDTSYVRRGRLRSADDVLKEIARYRDFLQAAGGGVTLSGGDPLFQPDFADA
                     VLKGCRKLGLHTCLDTSGHLGVNAGEEMLADTDLVLLDIKAWNPKRYRALTGGELRPTL
                     EFAERLASLRKPVWLRYVLVPGVTDNLEEIEELSRHAALLGNVERVDVLPFHQLGRFKW
                     DELKLDYTLKDVPEASAELAEQVRGIFRRGGFPDCVH"
     CDS             complement(3008..4012)
                     /locus_tag="ctg181_3"
                     /transl_table=11
                     /translation="APQDKKTAALLENMELKPYRILVAVPRALDEALATVPLIRALKTV
                     RCDMQVNIVCPSAQLGIWKTVPEVTHVLPHDSLKQLREALAADEFYNDGPLDLGVMLDG
                     DMETLKALEPYGPMMFSGLDTHPGARKYKFRVKAPVLRAAPPAHRVQLYLQLGGLHGLD
                     VATPSLFPVKKAAPAEDAPILIAPFSRLGSASEWSWEQWRELVSLLPGRTVLAALEEDR
                     ERAASLAEHLNVELAVGAPEALFPVMDGAAAAVAVDGDFPSLCSFRGLPVVTLFSTRLP
                     DVYRPMGTFNRSLYSHQCCSPCFRKECDRDVPCNRHIAVRDVLEALREISGKE"
ORIGIN
        1 ttccgatctc cccctgaaac gggccctgat gccctttggc ggcttgcgca tggcccaaca
       61 ggccatggaa tcctacgggt tcaagatgtg cgagaaaacc gcagacattt tcaaaaagat
      121 acgcaagacg cacaatgaag gcgttttcga cgcctacact tccgatatcc gggcggcccg
      181 ttccgccggg attattaccg gccttccgga cgcctatggc cgcggccgca tcatcgggga
      241 ctaccgccgg gtggccctgt acggcacgga taagctgatt gccgaacgcc gcaaggacct
      301 caaaaaccgt gaacattccc cccttacgga tgagctgatc cgtctgcgcg aagaaatgag
      361 cgaacaaata cgcgccctgg aagaactcgc ccagctgggc gcctcctacg gctgcgacct
      421 cactcgcccc gccgcaaacg cccgcgaagc cgtgcaatgg acgtacctgg gctacctagc
      481 cgccgtgaag gaacagaacg gagcggccat gtccctgggc cgcgtttcca ctttcttcga
      541 catttatttc acccgcgacc tggaacaggg gctcatcacg gaagaggaag ttcaggaaat
      601 catcgaccag ttcgtgatga agctgcgcat cgtccgtttc atccgcacgc ctgattacaa
      661 taacctgttc tccggagacc ccacctgggt gacggaatcc atcggcggca tgggagagga
      721 cgggcgcacc ctcgtcaccc gcagttcctt ccgcatgctg cagaccctgt acaacctagg
      781 gccggctccg gaaccgaacc tgaccgttct gtggtcccgg aacctgccgg aagccttcaa
      841 aagcttctgc gccaaggttt ccattgaaac atcctccgtc caatacgaga acgatgacct
      901 gatgcgccca cattggggcg acgactacgg catcgcatgc tgcgtgtccg ccatgcgcat
      961 cggcaagcag atgcagttct tcggagcgcg cgccaacctc gccaagtgcc ttctctacgc
     1021 cctgaacggc ggcgtggatg aactcaaggg caagcaggtg gccccgccct ccccccgcta
     1081 cacggaggaa attctcaact atgatgaagt gatggccctg tacgacaaga tgcaggactg
     1141 gctcgccaaa acttacattg acgcgctgaa catcatccac tacatgcacg acaaatactg
     1201 ctatgaacgc attgaaatgg cgctgcatga tccggaaatt ctgcgcacga tggctacggg
     1261 aatcgccggg ctttccgtgg cggcggactc cctgtccgcc atcaagtatg ccaccgtaaa
     1321 agccatccgc nnnnnnnnnn tccgcaatga agaagggctg attgtggact tcaagacgga
     1381 aggggaattc ccctgttacg ggaacaatga cccgcgcgtg gacgacatag catgcagcct
     1441 ggtcagcaat ttcatggaaa aactgcgcag gctccacact taccgcaatt ccctgcccac
     1501 ccagtccatc ctgacgatca cctccaacgt ggtgtacggc aaaaagacgg gcaacacgcc
     1561 ggacggccgc cgagccgggg aaccgttcgc ccccggagcc aaccccatgc acggaaggga
     1621 caggaacgga gcagtggcct ccatgctctc cgtagccaag ctgtcctatg acgactccct
     1681 ggacggcatc tcctacacct tctccatcgt tccccaggcc ctgggcaagg aggaacgtga
     1741 acgccgcgtc aagctcgtct ccctgctgga cgcctacttt gccgctacag gtcaccacat
     1801 taatgtgaac gtactggaac gggaaacgct tctcgacgcc atggatcacc cggaaaaata
     1861 cccgcagctt accatccgcg tttccggcta tgccgtgaat ttcatcaagc tgacccggga
     1921 acagcagcag gaggtcatca accgcacttt ccacacccgt taacccggac tgcaagcgaa
     1981 tgccgcaggt ttttccacaa aaccgtgatc cggccggaga aagctccgtc accggtttgg
     2041 ttcattccgt ggaatcctgc ggcaccgtgg acggccccgg catccgcttc gtccttttcc
     2101 tgtccggttg cagcctacgc tgccgctact gccataatcc ggatacctcc tacgtgcgga
     2161 ggggccggct caggagcgca gatgatgtcc tcaaggaaat agcccgttac cgggattttt
     2221 tgcaggcggc gggcggcggc gtcaccctct ccggcgggga ccccctcttc cagccggatt
     2281 ttgccgatgc cgtgctgaaa ggctgccgga aactgggcct gcacacctgc ctggacacct
     2341 ccggccatct gggcgtgaat gcgggcgaag aaatgctggc ggatacggat ctggtcctgc
     2401 tggatatcaa ggcatggaat ccgaaacggt accgcgccct gacaggaggg gaactgcgcc
     2461 ctacactgga gtttgcggaa cgcctggcct ccctccgcaa gcccgtgtgg ctgaggtatg
     2521 tgctggtacc tggcgttacg gacaatctgg aagaaattga ggaactctcc cgccatgcgg
     2581 cgcttctggg caacgtggaa cgcgtggacg tgctcccttt ccaccagctg gggagattca
     2641 aatgggatga attgaagctg gactatactt tgaaggacgt cccggaagcg tccgcggaac
     2701 tggcggaaca ggtccgcggc attttccggc ggggcggctt tccggattgc gtgcattaac
     2761 cggatcttcg cgcttcggcg ccaccaacag cagcaaaccg gacgcaggca tccccctctt
     2821 ccggatgagg ggccctctcc cctccactgt agaatccatg caaaaaggaa gacacatttc
     2881 tggaattgct ccggaatacg gcatttccgc aattcctttc gttatttcat cattctttac
     2941 ggcaacggca gcattcccgc cgttttttcc gccaacgccg ggggacggca ttccgtccct
     3001 tcccagatta ttctttcccg gaaatttccc gcaaggcttc cagtacgtcc cgcacggcaa
     3061 tgtggcggtt gcagggaaca tcccggtcac attccttccg gaagcaggga gaacagcact
     3121 ggtggctgta aagggaacgg ttgaatgtgc ccatagggcg gtaaacatcc ggaagccggg
     3181 tggaaaacag agtcaccacc ggaagccccc ggaaagaaca gagggacgga aagtcgccat
     3241 ccacggctac tgccgccgca gcgccgtcca tgacgggaaa gagagcttcc ggcgcaccca
     3301 ccgccagctc cacattcaaa tgttccgcca gggacgcggc gcgctcccgg tcttcttcca
     3361 gagccgccag caccgtccgt cccggcagaa gggaaaccag ctccctccac tgctcccagc
     3421 tccattcact ggcgctcccc aggcgggaaa acggagcgat taaaatgggg gcgtcttccg
     3481 ccggggccgc ttttttcaca gggaacaaag acggggtggc gacgtccagc ccatgcagcc
     3541 cccccaattg cagataaagc tgcacccggt gcgccggcgg ggcggcgcgc aggacggggg
     3601 cctttacccg gaatttgtac ttccgggcac cgggatgcgt gtccagcccg gaaaacatca
     3661 tggggccata cggttccagc gctttcaggg tttccatatc cccatccagc atgacgccca
     3721 gatccagcgg tccgtcattg taaaattcat ctgctgccaa agcttcccga agctgcttca
     3781 gggagtcgtg cggcagcaca tgggtaactt ccggcaccgt cttccatatc cccagctggg
     3841 cagacgggca gacgatgttc acctgcatgt cgcaccggac ggttttcaaa gcacgaatca
     3901 ggggaacagt cgccagggct tcatccagcg ccctgggaac ggctaccaga atgcggtacg
     3961 gcttcagctc catattttcc aggagcgcag ccgttttttt atcctgcggg gc
//
