LOCUS       c00425_gnlPro..         3389 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|EAIGGLJG_425.
ACCESSION   c00425_gnlPro..
VERSION     c00425_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 04:45:44
            Original ID  :: gnl|Prokka|EAIGGLJG_425
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3389
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..366
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg425_1"
                     /sec_met_domain="TPP_enzyme_C (E-value: 1.3e-29, bitscore:
                     91.1, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGDGGLQMNIQELGTIMEQKAPVKIICLNNNYLGNVRQWQAMFFN
                     RRYSFTPMLNPDYMKVASAYDIPSKRVFTREELKEAIAEMLATDGPFLLEACVVEEGNV
                     LPMTPPGGSVNQMLLEC"
     protocluster    1..3389
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:2838]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..2838
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
     cand_cluster    1..3389
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /kind="single"
                     /product="TPP_AA_metabolism"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3389
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_AA_metabolism"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and not (PFL-like or PFL_6_pcresol or
                     PFL_9_tma or Gly_radical or Gly_430_pcre or Gly_8-9_TMA or
                     PFL_acetate1 or PFL_acetate2 or Gly_radical_scatole or
                     PFL_proline or PFL_toluene) and minimum(2, [ALS_ss_C,
                     AlaDh_PNT_C, Arginase, BKACE, BcrAD_BadFG, Beta_elim_lyase,
                     CPSase_L_D2, Cys_Met_Meta_PP, ELFV_dehydrog, GRDB,
                     Glu_syn_central, Glu_synthase, Gly_reductase, HAAA1, HG2B1,
                     HG2B2, HGD-D, HleuD1, HleuD2, HleuS1, HleuS2,
                     Homoserine_dh, ILVD_EDD, IlvC, Lactoyl-CoA1, Lactoyl-CoA2,
                     Lyase_aromatic, MAAL_C, OKR_DC_1_5_lysine,
                     OKR_DC_1_5_ornithine, OKR_DC_1_7_arginine, OKR_DC_1_C,
                     OTCace, PALP, Proline_reductase, Sacchrp_dh_C, TPL,
                     Thr_dehydrat_C, TnaA, Urocanase]) and not (POR or POR_N or
                     PFOR_II))"
                     /tool="gutsmash"
     CDS             417..980
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ALS_ss_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg425_2"
                     /sec_met_domain="ALS_ss_C (E-value: 3.3e-16, bitscore:
                     47.5, seeds: 685, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDKTLYTIIVHSENIAGLLNQVTAVFTRRQINIESLNVSASSIK
                     GVHKYTITAWTDKDIIEKVVKQIEKKIDVIQAHYFTEDEIYFHEIALYKVSTPAFQENP
                     EASKLIRRYNARIVEVNPVFSIVEKNGMSEDIXXXXXXGELKALNCVLQFVRSGRVAIT
                     TSCFERVNEFLDGREAMYNQSKNQ"
     CDS             986..1729
                     /locus_tag="ctg425_3"
                     /transl_table=11
                     /translation="MSEENKIGTYQFVAEPFHVDFNGRLTMGVLGNHLLNCAGFHANDR
                     GFGIATLNEDNYTWVLSRLAIELDEMPYQYENFSVQTWVENVYRLFTDRNFAIIDKDGK
                     KIGYARSVWAMINLNTRKPADLLTLHGGSIVNYVCDEPCPIEKPSRIKVTSDQSIATLT
                     AKYSDIDINGHVNSIRYIEHILDLFPIELYKTKRIRRFEMAYVAESYFGDELSFFCDEV
                     NANEFHVEVKKNGNEVVCRSKVIFE"
     CDS             1795..2838
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: IlvC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) IlvN"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg425_4"
                     /sec_met_domain="IlvN (E-value: 1.3e-47, bitscore: 149.6,
                     seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="IlvC (E-value: 7e-41, bitscore: 128.0,
                     seeds: 295, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAQLNFGGTVENVVIRDEFPLEKAREVLKNETIAVIGYGVQGPGQ
                     ALNLRDNGFNVIVGQRQGKTYDKAVADGWVPGETLFGIEEACEKGTIIMCLLSDAAVMS
                     VWPTIKPYLTAGKALYFSHGFAITWSDRTGVVPPADIDVIMVAPKGSGTSLRTMFLEGR
                     GLNSSYAIYQDATGNAMDRTIALGIGIGSGYLFETTFIREATSDLTGERGSLMGAIQGL
                     LLAQYEVLRENGHTPSEAFNETVEELTQSLMPLFAKNGMDWMYANCSTTAQRGALDWMG
                     PFHDAIKPVVEKLYHSVKTGNEAQISIDSNSKPDYREKLEEELKALRESEMWQTAVTVR
                     KLRPENN"
ORIGIN
        1 atgggtgacg gcggactgca aatgaacatt caggagctgg gtacgattat ggagcagaag
       61 gctccggtga agattatctg cctgaacaat aattatctcg gaaacgtacg tcagtggcaa
      121 gctatgtttt tcaaccgtcg ctattcgttt accccgatgt tgaatccgga ttatatgaaa
      181 gtggcctcgg cttatgatat tccttccaag cgtgttttca ctcgtgaaga actgaaggaa
      241 gctattgctg aaatgctggc aacggatggt ccgttcctgc tcgaagcttg cgtggtggaa
      301 gaaggcaatg ttttgccgat gactcctccg ggggggtcgg taaaccagat gttgctggag
      361 tgctaggaga agttgagaat ggagagttga gagttcgcat ttaaaaaatt ggagttatga
      421 gtgataaaac gttatataca atcattgttc attcggagaa tatagctgga ttgctgaatc
      481 aggtaactgc tgtatttacc cgtcggcaaa taaatattga gagtctgaat gtatctgctt
      541 cttctattaa aggtgtacat aagtatacaa taacagcctg gactgataag gacatcattg
      601 agaaggtagt gaaacagatc gaaaagaaga tcgacgtgat tcaggcacat tatttcacag
      661 aggatgagat ttatttccac gagattgcat tatataaggt gtctactcct gcttttcagg
      721 agaatccgga agcatccaag ctgattcgta gatacaatgc acgtatagtg gaagtcaatc
      781 cggtattttc tattgtcgag aaaaatggaa tgagcgaaga tatannnnnn nnnnnnnnnn
      841 nnggtgaact gaaggctttg aattgtgttt tgcagtttgt tcgttcagga cgtgtagcga
      901 ttactaccag ctgttttgaa cgggtgaatg agtttctcga tggacgggaa gctatgtata
      961 accaaagtaa aaaccaataa gaataatgag tgaagaaaat aaaataggga cttatcagtt
     1021 tgtggcagag ccgttccatg ttgacttcaa cggacgtctg actatggggg tactgggaaa
     1081 tcatctactg aattgtgcag gttttcacgc caatgaccgt ggttttggta ttgcaacgct
     1141 gaatgaggac aattatacat gggtgctttc acgtctggct attgagttgg atgaaatgcc
     1201 ttatcagtat gagaatttct cggttcagac gtgggtggaa aatgtctacc gtttgtttac
     1261 ggaccgtaat tttgctatta ttgataagga tgggaagaag attggctacg cacgttccgt
     1321 atgggcaatg attaatctta atacccgcaa gcctgccgac ttgttgactt tgcatggggg
     1381 aagtatcgta aactatgtct gtgatgagcc ttgtccgatt gaaaagcctt cacgtatcaa
     1441 agtgacaagc gatcagtcga tagctaccct gacggcaaag tacagtgata tagatatcaa
     1501 tggtcatgta aacagcatcc gttacatcga acatatcctt gatttgttcc cgattgaatt
     1561 gtataagacc aaacgtatcc gtcgttttga aatggcgtat gtggcggaaa gctattttgg
     1621 tgatgaactc tctttcttct gcgatgaagt gaatgcaaac gagtttcacg tagaagtgaa
     1681 gaagaatggc aacgaagttg tttgccgctc gaaagtgata tttgaataaa tgtgagaata
     1741 taaaatcaat taataacaga tggcgtaagt catcaaaaac taaaaacaaa aaaaatggca
     1801 cagttgaatt ttggcggaac tgtagaaaat gtagttatcc gtgatgaatt tccattggaa
     1861 aaagctcgtg aagtattgaa aaatgaaaca atcgctgtaa tcggttatgg cgtacaaggt
     1921 cccggacagg ctctgaacct tcgtgataac ggtttcaatg taatcgttgg tcaacgccag
     1981 ggaaagacat atgacaaagc ggtagctgac ggatgggtcc cgggtgaaac tttgttcggt
     2041 attgaagaag cttgcgaaaa aggtacgatc attatgtgcc tgttgtctga tgcagcagta
     2101 atgtctgtat ggcctactat caagccttac ctgactgcag gaaaagctct ttatttctct
     2161 catggttttg ctattacatg gagtgaccgc acaggtgtag ttcctcctgc agatatcgac
     2221 gtaatcatgg ttgctcctaa aggttcgggt acatccttgc gtactatgtt ccttgaaggt
     2281 cgcgggttga actcttcata cgctatctat caggatgcaa caggcaacgc tatggacaga
     2341 acaatcgcat tgggtatcgg tatcggttca ggttatttgt tcgaaacaac tttcatccgc
     2401 gaagctactt ccgacctgac aggcgaacgt ggttcattga tgggagctat ccagggtctg
     2461 ttgctggcac aatacgaagt gttacgtgaa aacggtcaca ctccttccga agcattcaac
     2521 gaaactgtag aagagctgac tcagtcattg atgccgttgt ttgcaaagaa cggtatggac
     2581 tggatgtatg ctaactgctc tactacagct caacgtggtg ctctcgactg gatgggcccc
     2641 ttccacgatg ctatcaaacc ggtcgttgaa aagttgtatc acagtgtgaa gactggtaac
     2701 gaagcacaga tttcaatcga ctctaactcc aaaccggatt atcgtgagaa actggaagaa
     2761 gaactgaaag cattgcgcga aagcgaaatg tggcagactg ccgtgacagt tcgtaaactt
     2821 cgtccggaaa ataattaatc agacttatac tatataagtt tttcaaaagg cgggaaaagc
     2881 gtattttccc gccttttcta tgtagatacc ggtcttttga gtaatagaaa aaggatgaaa
     2941 gtagaaaaaa agattaaaag atactgataa tataggtgaa aaagagcatt tatgattacc
     3001 tttacggcaa caaataaaac agaataggca atgaagaaac tttttactta cagaagatcg
     3061 atacgtatga agatgatttc ttctttaatg aatagtatat cagaatgtta cattaaaaga
     3121 tatttaagtt attctctacc gaatggtagc tgtaatacca tttcaagtcc tttttatatt
     3181 tcgtgttcca ttagtgattc gttgatatga tgcagaattt tattctgtag caggaaaaag
     3241 tatgcgttaa attgaattta agacagagct tttggaacag aatttagtgg gaaaataaca
     3301 ttgtttgttt tgtttgtaaa tgggagtcct ctttcgtgag aaagggggct tcttttttta
     3361 tcccgatgat tcaggaggat accgcttcc
//
