LOCUS       c00392_gnlPro..         3247 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NLMMPNLH_392.
ACCESSION   c00392_gnlPro..
VERSION     c00392_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 03:50:03
            Original ID  :: gnl|Prokka|NLMMPNLH_392
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3247
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..711)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 75.5;
                     E-value: 1.6e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg392_1"
                     /sec_met_domain="Radical_SAM (E-value: 3.5e-32, bitscore:
                     99.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCPFRCLYCANPDTIDVKGG
                     TPTTPEEILQMAISQKAFFGKKGGITFSGGEPTLQAEALIPLFKELKANGIHICLDSNG
                     GIWNEKVEELFSLTDLVLLDIKQFNPERHRTLTGRSNEQTLRTAAWLEEHEHPFWLRYV
                     LVPGYSDSEEDIRLLGESLGKYRQIQRVEILPYHRLGVHKYEVMGWDYKLKEVKENTPE
                     QLERAEKLFKEYFTN"
     protocluster    1..3247
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:2941]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..2941
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3247
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3247
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(713..2941)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg392_2"
                     /sec_met_domain="Gly_radical (E-value: 1.1e-47, bitscore:
                     148.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1171.1, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKTFTNGLWNKEINVSDFVSKNIAPYTGDASFLQGPTERTKR
                     IWDICLKALEEERNNNGVRSLDNTTVSTITSHKAGYIDKDNELIVGLQTDELLKRSIKP
                     FGGINVVAKACRENGVEVDDKVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGIDRLIEAKQEDLRNLTGPMTEARIRLREEVAEQIKALKDIK
                     VMGEYYGLDLSHPATSAQEAVQWVYMAYLAAIKEQDGAAMSLGNVSSFLDIFIEYDLAH
                     GKINETFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTEAIGGRFNDGRVKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKDFCAKVSVDTSSIQYENDNLMREVRNC
                     DDYGIACCVSYQAIGKQIQFFGARANLAKALLLAINGGRCENTGTVMVKGIPVLTGETL
                     KFEEVMANYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMAFIDTNPRINLAYGVAGL
                     SIAIDSLSAIKYAKVTARRNEIGLTEGFDTEGSFPCFGNNDDRVDHLGVDLVYYFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGPTPEERVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLEDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
ORIGIN
        1 attcgtgaaa tactctttga acagtttttc tgcccgttcc agttgttccg gagtattttc
       61 cttcacttct ttcaatttgt aatcccatcc catgacttcg tatttgtgaa cgcccagacg
      121 gtggtaagga agtatttcca cccgttgtat ctgccggtat ttgccgagac tttcacccag
      181 caggcggatg tcttcttccg aatcgctgta accgggaacc agtacataac gcagccagaa
      241 ggggtgttca tgttcttcga gccaggcagc ggtgcggagt gtctgttcgt tgcttcgtcc
      301 cgtcagcgta cggtggcgtt cgggattgaa ttgcttgatg tccagcagca cgaggtcagt
      361 caggctaaag agctcttcca ccttttcatt ccagatgcct ccgttactgt ccagacagat
      421 atggatgccg ttcgccttca gctccttgaa aagaggaatc agcgcttctg cttgcagggt
      481 aggctcaccg ccggagaaag taatacctcc tttcttcccg aagaatgcct tttggctgat
      541 agccatttgc agaatctctt ccggagtggt gggagtgcct ccttttacat caatcgtatc
      601 cggattggca caatacagac aacggaacgg acatccttgc aggaaaacga cgagccggag
      661 acccggcccg tcgaatgttc ccatactttc gtaggagtgt acatttatca tattacattc
      721 tttcgtggaa tgaacggctg atgacttcca actgatgctc gcggctcaac ttcacgaagt
      781 tcacggcata gccggaaacg cggatggtga gttgcggata cttttcagga tgttccatgg
      841 catcttccag catttcgcgg ttcagtacgt ttacgttcaa atggtgggca cctttggtga
      901 agtagccgtc catcatggtt accaggttct ctacacgttc ttccggtgtc gggcccagtg
      961 atttaggaac aatggagaac gtattgctga tgccgtcctg cgagtcgcgg tagcgcaatt
     1021 tggctacgga gctcaaggaa gcgatggcac cgttcttgtc acgtccgtgc attgggttgg
     1081 cacccggagc aaaggcaaca cctttggcgc gtccgtccgg agtggcaccg gtcttcttgc
     1141 cgtacatcac gttggaggtg atagtcagca aggaaagggt gggacgggca ttcttgtaga
     1201 caggcagctt tttcagttct tcgctgaaat agtaaacgag gtctactccc aggtggtcca
     1261 cacggtcgtc attattgccg aagcagggga atgagccttc agtgtcgaaa ccttcggtca
     1321 ggccgatttc gttgcggcgt gcagtgactt tggcatattt gatggcggac agtgagtcta
     1381 tggcgatgga aaggccggca acaccgtatg ccagattgat gcgtgggttg gtatcgatga
     1441 atgccatctg ggccttctca taataatact tatcatgcat gtagtggatg atattcatgg
     1501 cctcgttgta gacacgggct atttcagtca gtaccttctt gtagttggcc ataacttcct
     1561 caaacttcag tgtctcgccg gtcaatacgg gaataccttt caccatgaca gttccggtgt
     1621 tttcgcaacg gccaccgttg atggcaagca gcagggcctt ggcaaggttg gcacgtgcgc
     1681 cgaagaactg gatttgcttg ccgatggctt ggtaggatac acagcaggca atgccgtaat
     1741 cgtcgcagtt gcgcacttcg cgcatcaggt tgtcgttttc gtactggatg gagctggtat
     1801 ctacggaaac tttggcgcag aaatccttga agccttccgg cagttcggga ctccacagta
     1861 cggtcaagtt tggttccgga gaaggaccga ggttgtatag cgtctgcaag aagcggaaag
     1921 aagtcttggt gactttcacg cgtccgtcat tgaagcgtcc tccgatggct tcggtcaccc
     1981 aagtcgggtc accggcaaag atatcattgt acgactgcat acgcagatgg cgtaccatgc
     2041 gcagcttgat gacgaactgg tctatcagtt cttgcgcaaa cgtctcattg atttttccgt
     2101 gagccagatc gtattcaatg aatatatcga ggaacgaaga tacgttaccc aatgacatgg
     2161 cagcaccgtc ttgctctttg atggcagcaa gatatgccat gtatacccat tgcacggcct
     2221 cttgagctga tgtagccgga tggctgaggt cgagtccgta atattcgccc ataaccttga
     2281 tgtccttcaa agctttgatt tgttccgcca cttcttcgcg tagacggatg cgtgcttcgg
     2341 tcatcgggcc ggtgaggttg cgcaagtcct cctgcttggc ttcaatcaga cggtcgatgc
     2401 cgtagagagc caatcggcgg tagtcgccga tgatacgtcc gcgggcatag ttgtcgggca
     2461 atccggtaag gaatcccagt gagcggaagg aacggatttc ttcggtatat acatcgaata
     2521 cgccgtcgtt atgtgttttg cggtaatggg tgaagatgtc ttttacttta tcgtccactt
     2581 ccactccgtt ttcacggcat gcttttgcta ccacgttgat gcctccgaaa ggtttgatgg
     2641 atcgtttcaa cagttcatca gtctgcaaac cgacaataag ttcattgtcc ttatcaatat
     2701 acccggcttt atgagaagtg atggtagata cggtggtatt gtccagtgaa cggacgccat
     2761 tgttgttcct ttcttcttcg agagccttca gacagatgtc ccagatacgt ttggtacgtt
     2821 cggtaggtcc ctgcaagaaa gatgcatccc cggtataggg tgcgatgttt ttactgacga
     2881 aatcactgac attgatttct ttattccaaa gcccattggt aaaggttttg ttcaattcca
     2941 taaaatttgt ttttgttagt aggttagata atgttatcct ttagagcgca caaaaatact
     3001 gaataaattc cgtaatttta taagagatac ttctcttttt atgttgaaca gcataaaata
     3061 actattatta ggtagtgaat gtccggggga gtagagatgt taatactttc tatatgtggt
     3121 ttatgaattg aaacaagaac cgaataataa tctgcattat ttacaaatga tgtaggttta
     3181 aggaacagtc gtattctgaa cctaattcag tcgtatagtg aatgccattc agtcgtatac
     3241 tgaatag
//
