LOCUS       c00343_gnlPro..         2820 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|GAMIKDJE_343.
ACCESSION   c00343_gnlPro..
VERSION     c00343_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 09:37:02
            Original ID  :: gnl|Prokka|GAMIKDJE_343
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2820
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2820
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[804:2818]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      805..2818
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..2820
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2820
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             3..149
                     /locus_tag="ctg343_1"
                     /transl_table=11
                     /translation="MKILSYQQVQTRAVAQAGAGDGGGEDPDKNPDSGGDGSGDEEAPD
                     PTV"
     CDS             363..812
                     /locus_tag="ctg343_2"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRVDCDFTAKDVDTAHRYRGFSCWGYHYYIRK
                     SGEIEPMRDEDTVGAHARGYNAISLGVCYEGGLDENGKAADTRTPRQKEALHRLVHELL
                     QRYPEAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVIGEFSIN"
     CDS             complement(805..1533)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.5;
                     E-value: 1.4e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg343_3"
                     /sec_met_domain="Radical_SAM (E-value: 7.4e-30, bitscore:
                     92.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGITFSGGEPTFQAKALVPLVRELKEKGIHVCLDSN
                     GGLWNEDVEELFRLTDLVLLDIKEFNPNRHQTLTGRSNEQTIRTAAWLEEQEKPFWLRY
                     VLVPGYSDFEEDIRALGEALGKYKMIQRVEILPYHTLGVHKYEAMGQEYKMKGVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(1547..2818)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg343_4"
                     /sec_met_domain="Gly_radical (E-value: 1e-47, bitscore:
                     149.3, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 1.6e-175, bitscore:
                     572.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="TWVTESLGGRLNDGRTKVTKTSFRFLQTLYNLGPSPEPNLTVLWS
                     PELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQSDDYGIACCVSYQEIGKQIQFFGAR
                     CNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTLKFEEVMDNYKKVLTEIARVYNEAM
                     NIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGLSIALDSLSAIKYAKVTARRNDIGL
                     TEGFDIEGEFPCFGNDNDKVDHLGVDLVYFFSEELKKLPVYKNARPTLSLLTITSNVMY
                     GKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIASLSSVAKLRYRDSQDGISNTFSIVP
                     KSLGATDEDRIENLVTMMDGYFTKGAHHLNVNVLNRDMLYDAMEHPENYPQLTIRVSGY
                     AVNFVKLSREHQLEVISRSFHERM"
ORIGIN
        1 tgatgaagat tctgagttat cagcaagtgc agactcgtgc ggtggcgcag gcgggtgcag
       61 gcgatggcgg gggagaagac cctgacaaga atccggattc cggtggtgat ggctccggtg
      121 atgaagaggc tcccgaccct acggtataat tggcagacgg gtcaaggtaa taacgaatta
      181 gaataacttc cattaatatt ttgataagat gaaagaaata gtaaaccgga ttttagatgt
      241 gatcatgtat ctgattcctt tcttcggaaa gagaaagcgg gacagggtgg tgagagaagt
      301 gcgctatcat actacctgta aggaggtgtg caaagtgaaa acgacggaaa gggaaaagga
      361 acatgagaaa gattagtctg attgtgatcc attgttccgc cacccgtgtc gattgcgact
      421 ttacggcaaa ggatgtggat acggctcacc gttatcgtgg cttttcgtgc tgggggtatc
      481 attattatat acgcaagtcg ggcgagattg agccgatgcg ggatgaagat acggtcggag
      541 ctcatgcacg tggctataat gcgatcagct taggtgtatg ttatgagggc ggtctggatg
      601 agaatggaaa agctgcggat acgcgtacac cccgtcagaa ggaggcgcta catcgcttgg
      661 tacacgaatt gttgcagcgt tatccggaag ctaaagtagt agggcatcgg gatttaagtc
      721 ccgacaccaa ctataatggg atcgtagatc cttgggaacg gattaaagaa tgcccttgct
      781 ttgaggtcat tggagaattc tccattaatt aactaccacc gtagtgaaat actctttgaa
      841 tacctccgca gctttttcaa gctgttcggg ggtattttct tttacccctt tcatcttgta
      901 ttcctgtccc atcgcttcgt acttatgtac gcccagtgta tggtaaggta gtatttccac
      961 ccgttggatc atcttgtatt ttccgagtgc ttctcccagt gcgcgtatgt cttcttcaaa
     1021 atcgctgtat ccgggcacta gcacgtagcg tagccaaaaa ggtttctcct gttcttccag
     1081 ccaggcggcc gtgcggatgg tttgctcgtt gcttcttccg gtgagcgtct gatggcggtt
     1141 ggggttgaat tcctttatat ccaataacac aagatcggtc agtctgaata attcttctac
     1201 gtcttcgttc caaagtcctc cgttgctgtc cagacagaca tggatgcctt tttctttcag
     1261 ttcgcggacc agcgggacga gtgcttttgc ctgaaacgtc ggttcgcctc cggagaaagt
     1321 gattcctcct cgtttcccga agaaggggcg ctggctcatt gccatgcgga cgatttcttc
     1381 cggtggggta ggcgtgcctc cctttcctgc tatcgtatcc ggattggcgc aatagaggca
     1441 gcggaagttg catccttgaa gaaaaacgac gagccgtaag cccggcccgt cgaatgttcc
     1501 catactttcg tatgaatgta cgtttatcat catatagagt aatagattac atacgttcgt
     1561 ggaagctacg gctgataact tccagttggt gttcgcggct cagcttcacg aagtttacgg
     1621 catagccgga aacgcggata gtcagctgcg gatagttttc cggatgctcc atggcatcgt
     1681 acagcatatc acggttcagt acgtttacgt tcaggtggtg ggcacctttg gtgaagtaac
     1741 cgtccatcat cgttacgagg ttctcgatac ggtcttcgtc cgtagcaccc agtgatttcg
     1801 gaacgataga gaaggtgttg ctgataccgt cctgtgagtc acggtaacga agtttcgcta
     1861 cagagctcag agaagcgatc gcaccattct tgtcgcgtcc gtgcatcggg ttggcacccg
     1921 gagcgaaggc aacacctttg gcacgtccgt cgggagtagc accggtcttt ttgccgtaca
     1981 tcacattgga agtgatagtc agcaaggaga gggtaggacg ggcattcttg taaacaggca
     2041 atttcttcaa ttcttcgctg aagaagtata ccaggtctac gccaaggtgg tctaccttgt
     2101 cgttgtcgtt accgaagcaa gggaattctc cttcgatgtc gaagccttct gtcagaccga
     2161 tatcattgcg gcgggcagtt actttcgcat atttgatggc agacagtgaa tcgagggcga
     2221 tggaaagtcc ggctacaccg taagcaaggt ttatgcgcgg gttcgtatct acaagagcca
     2281 tctgggcctt ctcgtagtaa tacttgtcgt gcatatagtg gatgatgttc atcgcttcgt
     2341 tatatacgcg ggcgatttcg gtcagcactt ttttgtagtt gtccattact tcttcgaact
     2401 tcagcgtgtc gctggtcagc acggggatgt tcttcaccat gactgtaccg gtattctcgc
     2461 aacgtccgcc gttgatggca agcaacaggg ctttggccag gttgcaacgg gcgccgaaga
     2521 actgaatctg cttacctatt tcctggtatg atacgcagca ggcgattccg tagtcgtcgg
     2581 actgacgtac ttcgcgcatc aggtcgtcat tttcgtactg gatagaagaa gtgtcgatag
     2641 aaacctttgc acagaattct ttgaatcctt ccggaagttc cgggctccag agtacggtca
     2701 ggttcggttc cggtgaaggg ccgaggttgt acaatgtctg caggaaacgg aaggaggtct
     2761 ttgtcacttt ggtacgtccg tcgttgagac gtccgcccag agattcggtt acccaggtcg
//
