LOCUS       c00477_gnlPro..         1715 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|OAOCPAKH_477.
ACCESSION   c00477_gnlPro..
VERSION     c00477_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 09:50:47
            Original ID  :: gnl|Prokka|OAOCPAKH_477
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 1715
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..1715
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:1713]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..1713
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..1715
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..1715
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(3..539)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 55.9;
                     E-value: 1.8e-17)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg477_1"
                     /sec_met_domain="Radical_SAM (E-value: 3.6e-27, bitscore:
                     82.9, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQVFPQNHDPDGESSVTGLVHSVESCGTVDGPGIRFVLFLSGCS
                     LRCRYCHNPDTSYVRRGQLRSADDVLKEIARYRDFLQAAGGGVTLSGGDPLFQPDFAGA
                     VLKGCRKLGLHTCLDTSGHLGVNAGEEMLADTDLVLLDIKAWNPKRYRALTGGELRPTL
                     EFAERLASLRKPVWLR"
     CDS             complement(556..1713)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg477_2"
                     /sec_met_domain="Gly_radical (E-value: 2.9e-47, bitscore:
                     146.9, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.9e-116, bitscore:
                     376.9, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="EPNLTVLWSRNLPEAFKSFCAKVSIETSSVQYENDDLMRPHWGDD
                     YGIACCVSAMRIGKQMQFFGARANLAKCLLYALNGGVDELKGKQVAPPSPRYTEEILNY
                     DEVMALYDKMQDWLAKTYIDALNIIHYMHDKYCYERIEMALHDPEILRTMATGIAGLSV
                     AADSLSAIKYATVKAIRNEEGLIVDFKTEGEFPCYGNNDPRVDDIACSLVSNFMEKLRR
                     LHTYRNSLPTQSILTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDRNGAVASML
                     SVAKLSYDDSLDGISYTFSIVPQALGKEERERRVKLVSLLDAYFAATGHHINVNVLERE
                     TLLDAMDHPEKYPQLTIRVSGYAVNFIKLTREQQQEVINRTFHTR"
ORIGIN
        1 tacctcagcc acacgggctt gcggagggag gccaggcgtt ccgcaaactc cagtgtgggg
       61 cgcagttctc cgcctgtcag ggcgcgatac cgtttcggat tccatgcctt gatatccagc
      121 aggaccaggt ccgtatccgc cagcatttct tcgcccgcat tcacgcccag atggccggag
      181 gtgtccaggc aggtgtgcag gcccagtttc cggcagcctt tcagcacggc accggcaaaa
      241 tccggctgga agaggggatc cccgccggag agggtgactc cgccgcccgc cgcctgcaaa
      301 aaatcccggt aacgggctat ttccttgagg acatcatctg cgctcctgag ctggcccctc
      361 cgcacgtagg aggtatccgg attatggcag tagcggcaac gcaggctgca gccggacagg
      421 aaaaggacga agcggatgcc ggggccgtcc acggtgccgc aggattccac ggaatgaacc
      481 aaaccggtga cggagctttc tccgtccgga tcatggtttt gtggaaaaac ctgcggcatc
      541 cgcttgcagt ccgggttaac gggtgtggaa agtgcggttg atgacctcct gctgctgttc
      601 ccgggtcagc ttgatgaaat tcacggcata gccggaaacg cggatggtaa gctgcgggta
      661 tttttccggg tgatccatgg cgtcgagaag cgtttcccgt tccagtacgt tcacattaat
      721 gtggtgacct gtagcggcaa agtaggcgtc cagcagggag acgagcttga cgcggcgttc
      781 acgttcctcc ttgcccaagg cctgaggaac gatggagaag gtgtaggaga tgccgtccag
      841 ggagtcgtca taggacagct tggctacgga gagcatggag gccacggctc cgttcctgtc
      901 ccttccgtgc atggggttgg ctccgggggc gaacggttcc ccggctcggc ggccgtccgg
      961 cgtgttgccc gtctttttgc cgtacaccac gttggaggtg atcgtcagga tggactgggt
     1021 gggcagggaa ttgcggtaag tgtggagcct gcgcagtttt tccatgaaat tgctgaccag
     1081 gctgcatgct atgtcgtcca cgcgcgggtc attgttcccg taacagggga attccccttc
     1141 cgtcttgaag tccacaatca gcccttcttc attgcggatg gcttttacgg tggcatactt
     1201 gatggcggac agggagtccg ccgccacgga aagcccggcg attcccgtag ccatcgtgcg
     1261 cagaatttcc ggatcatgca gcgccatttc aatgcgttca tagcagtatt tgtcgtgcat
     1321 gtagtggatg atgttcagcg cgtcaatgta agttttggcg agccagtcct gcatcttgtc
     1381 gtacagggcc atcacctcat catagttgag aatttcctcc gtgtagcggg gggagggcgg
     1441 ggccacctgc ttgcccttga gttcatccac gccgccgttc agggcgtaga gaaggcactt
     1501 ggcgaggttg gcgcgcgctc cgaagaactg catctgcttg ccgatgcgca tggcggacac
     1561 gcagcatgcg atgccgtagt cgtcgcccca atgcgggcgc atcaggtcgt cgttctcgta
     1621 ttggacggag gacgtttcaa tggaaacctt ggcgcagaag cttttgaagg cttccggcag
     1681 gttccgggac cacagaacgg tcaggttcgg ttccg
//
