LOCUS       c00501_gnlPro..         2769 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|BLDPJHOP_501.
ACCESSION   c00501_gnlPro..
VERSION     c00501_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 05:02:46
            Original ID  :: gnl|Prokka|BLDPJHOP_501
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2769
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2769
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[869:2768]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      870..2768
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..2769
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2769
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(93..671)
                     /locus_tag="ctg501_1"
                     /transl_table=11
                     /translation="MKTPQEIAEMISKRFYPLPDADLDSLASIIESSKAAKGELLLDNG
                     QISKYLYYVESGLLRQFYYKNKRDITEHFSCEGNIVICIASTFQREPTRLLIEALEPTV
                     YHRISFSEFSDLLKTSLAINRLYQKILEWALMLSQEKADSWRFESARKRYLRFQQEYPE
                     AAKRASVNHIASYLLMTPESLSRVRAGVL"
     CDS             complement(870..1604)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 67.6;
                     E-value: 4.7e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg501_2"
                     /sec_met_domain="Radical_SAM (E-value: 3.8e-34, bitscore:
                     106.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWI
                     RHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPL
                     SKERLDNAKAIFSRYKPENNKA"
     CDS             complement(1605..2768)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg501_3"
                     /sec_met_domain="Gly_radical (E-value: 3.9e-47, bitscore:
                     147.0, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 4.2e-115, bitscore:
                     373.1, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="EPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRPDYGDD
                     YGIACCVSPMKIGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRGEYLEY
                     DEVMAKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGIAGLSI
                     VADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFMEYLRQ
                     HETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGAIAALA
                     SVAKLPFQHAHDGISYTFAVSPATLGKEREVQINNLVSLLDGYFTPDGGQHLNVNVFDK
                     DLLIDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
ORIGIN
        1 taaactgacg caaatataga tggattactt gaaccgtgaa atcaccacaa attgctattt
       61 tcacctcaat aatacctgaa tgtaggtaga gactacaaca ctcccgcccg cacccggctc
      121 aaagattcgg gtgtcatcaa taaatacgag gcgatatgat tgacagaagc acgtttcgct
      181 gcttccggat attcctgttg aaaacgcaga taacgttttc ttgccgactc aaaacgccaa
      241 gagtccgctt tctcttgcga aagcatcaaa gcccactcca gtattttctg gtacaatcta
      301 ttaatagcta aagatgtttt caataaatcg ctaaattccg aaaaagagat ccggtgataa
      361 accgtcggtt ccaatgcctc gattaataaa cgcgtcggtt ctctttggaa tgtgctggct
      421 atacaaataa cgatatttcc ctcacaggag aaatgctcgg taatatcacg tttattctta
      481 tagtaaaact ggcgtaataa tcccgattcc acataatata aatatttaga tatttgtccg
      541 ttatccaaca ataattctcc tttcgcagcc ttacttgatt ctatgatcga ggccagagaa
      601 tccaaatccg catcgggtaa tggataaaaa cgtttggata tcatttcggc tatttcttgt
      661 ggtgtcttca tactattcat cattttctga tctatatcat tttttatctc cttacaaaaa
      721 tagatatttg ttgcatataa caaaacaaac acatattaaa tagtcaaggt tagtctaggc
      781 aaaaaattat tactatcgtt taaaagctaa tcggattaga ttgttttccc tgattagctg
      841 ttgcataatc gcaaggcttg atcgagagat caggccttgt tattttctgg tttgtagcga
      901 gagaatatcg ctttcgcatt atctaaacgt tcttttgata agggttccac cccctttaac
      961 ggataatcga gacctaattg ctcatattta taggttccca tcgtatgata aggcaataat
     1021 tccactttgc gaaccacttt atatgaactg acataccgag ctaacgcctc cagccactcg
     1081 tcattatccg tatatccggg gacaataaca tggcgaatcc aagtatctat gccacgtctc
     1141 tccaattcat ccaagaacag aagcgtatta tcctgcttga cacccgccag taacggatgt
     1201 aaatccgggt ttaaggtttt gatgtccaat aacactaagt ccgcataatc caagacttcc
     1261 caagcttttg aggtacatac gaaaccagat gtgtccaaag ctgtgtgaag accttgctct
     1321 tggcacaaac ggaaaaactc tttcagaaac tcaggttgca ataaaggttc cccaccggta
     1381 acagtcaccc ccccacgggc gataaaactc ttataccgta agacttccgt caatagctcg
     1441 ccgggagtca tctgatattt caccttccct ttcgggttcc aagtatccgg gttatggcaa
     1501 tacaggcaac gcaacgggca gccctgcata aaaacgacaa agcgaattcc gggaccatct
     1561 acggtcccga aactctctaa tgaatgtatc ttacctttta tcacttacag tgaatggtta
     1621 atggttcttg aaataacatc caactgttgt tcacgagtca gtttcacgaa gtttaccgca
     1681 tatccggata cacggatcgt aagttgcgga tatttctcag gatgttccat agcgtcgatc
     1741 aacaaatcct tatcaaatac gttaacattc agatgctgac ctccatccgg agtaaaatag
     1801 ccatccaata agctaaccag attattgatc tgcacttcac gctcctttcc caacgtagcc
     1861 ggagaaacag cgaaagtata agagataccg tcgtgcgcat gctggaatgg aagcttcgct
     1921 accgaagcta gcgcagcgat agcacctttc gtatcacgac cgttcatagg gttcgcgccc
     1981 ggagcgaaag gagttccacc gatacggcca tccggtgtcg taccagtctt cttaccatat
     2041 accacattag aggtgatcgt aaggatggac tgcgtaggtt tcgcatggcg ataggtctcg
     2101 tgctgacgca aatattccat gaacttctcg gtgatctcta cagcgatact atccgtacga
     2161 tcgtcgttat taccgaaagg aacatattcg ccctcacgtt cgaaatcaac cgccaagcca
     2221 cgctcgtcac ggatcacacg tactttcgta tcacggatag cagccaacga atcggcaacg
     2281 atagaaagac cggcgatacc tgtcgcacgg atacgctcta catctccatc gtgaagcgac
     2341 atctcgaatg cctcatatgc gtatttatca tgcatataat ggattatctt caaggcgttc
     2401 acatacactt tcgccaacca gcgcatcatt tgctcatact tagccatcac ctcatcgtat
     2461 tccagatact cgccgcgaac cggctcgaac atcggggcta cctgtacgcc gctgcgctca
     2521 tcccgtcctc cgttgatcgc atataataaa catttcgcca aattcgcacg ggctccgaag
     2581 aactgcatct gcttaccgat cttcatagga gaaacgcaac aagcgatacc ataatcatct
     2641 ccataatccg gacgcatcaa atcgtcgttc tcatattgga tagccgatgt atcgatagag
     2701 accttggcac agaaacgttt ccagttctcg ggagcgttct taaaccacag aaccgtcaaa
     2761 ttgggttct
//
