LOCUS       c00243_gnlPro..         5709 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KOGNBGIP_243.
ACCESSION   c00243_gnlPro..
VERSION     c00243_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 04:07:56
            Original ID  :: gnl|Prokka|KOGNBGIP_243
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5709
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..396)
                     /locus_tag="ctg243_1"
                     /transl_table=11
                     /translation="MSMHEIESLVESSVITVATASPIPPLARNICFNLYQLQNQLDCGY
                     TVLRVREELEKLGYLFLLPPEQLPEPERSAALKLNEKGGFLSDGTYFDHRSGRCCVTAG
                     SLLWTKLIDLGILPESAKTELRELDPLE"
     protocluster    1..5709
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3821:5709]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3822..5709
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5709
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5709
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(521..2071)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg243_2"
                     /sec_met_domain="Pyr_redox_2 (E-value: 1.1e-47, bitscore:
                     151.5, seeds: 65, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLDSSMRNQLSGIFSGLTNEYIFDIQVSPEHESRKELLELLNDVA
                     SCSDKITVQVADGEGLEFSLLKNGEKTGIKFRGVPNGHEFTSLLLAILNSDGKGKNIPD
                     DSICNRVKALNGPVKLTTYVSLTCTNCPDVVQALNAMATLNPEISHEMVDGAINQEEVA
                     AMKVQGVPSVFADGKLIHVGRGDFGELLGKLEAQYGVKEENLSVVEKNYDVIIVGGGPA
                     GSAAAIYTARKGLAVAVVADRIGGQVKETVGIENMISVSQTTGQQLALDLMSHMKDYPI
                     DILEHRRIEKVEVEGREKLLTTSTGEKLIAPAVIVATGASWRRLNVPGESEYIGRGVAF
                     CPHCDGPFYKGKHVAVVGGGNSGVEAAIDLAGICSKVTLLEFADELKADQVLQDKARSL
                     PNVEIFMHSQTMEVLGNGEKVTGIRIKDRKTEEVRNIELDGIFVQIGLAANSGPFREVV
                     DTNRQGEIVIDSHCRTSVPGIYAAGDVSTVPYKQIIISMGEGAKAALTAFEDRMRGML"
     CDS             complement(2165..2731)
                     /locus_tag="ctg243_3"
                     /transl_table=11
                     /translation="MEPIINSQAPEFKVQAYHNGSFKTVSSDDIKGKWAIFFFYPADFT
                     FVCPTELVDMADKYDQFQAMGVEVYSVSTDSHFVHKAWHDASETIRKIKYPMLADPTGA
                     LSRAFGVMIEEDGMAYRGTFLVNPEGKIKLAEINDNGIGRNADELLRKVEAAQFIAEHP
                     SEVCPAKWKKGGETLKPSIDLVGKI"
     CDS             complement(2892..3371)
                     /locus_tag="ctg243_4"
                     /transl_table=11
                     /translation="MKDKDKDLNVKGAYLHMAADALVSVGVVVSGIVIAHTGWYIIDPI
                     IGLLIAIVIIVSTWGLLRDSIRLSLDGVPAGTDIDKVEKTIHSVEGVEGVHHLHIWAIS
                     TTETALTAHVSVADISEMERIKQEIKHDLQHVGIQHATLEFETSGFKCDGHCCSD"
     CDS             complement(3395..3736)
                     /locus_tag="ctg243_5"
                     /transl_table=11
                     /translation="MEQHHHDHHHHEITSLNKAFIIGISLNLAFVVAEFTAGFWYNSLG
                     LLSDAGHNLGDVASLVLAMLAFRLAKVHPTCSYTYGYKKSTVLVSLLNAVXXXSRVMRS
                     LGWQVSESL"
     CDS             complement(3822..4553)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.3;
                     E-value: 1.6e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg243_6"
                     /sec_met_domain="Radical_SAM (E-value: 6.5e-37, bitscore:
                     116.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LEIKGNIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWDS
                     KGLAKYQMTPEELLAEVLRYKNFIAKGGVTVTGGEPLLQAEFLREFFRLCREAGIHTAL
                     DTSGYICTPKALAVLEYADLVLLDIKTIDPDLHPRLTAVKLDNTLRFLDELEKRGVPVW
                     IRHVIVPGLTDNDEALDKLAEYISHYTVVQKAELLPYHTMGAYKYEAQGLDYKLKEVEP
                     LSAERLANAKEIFKKHGITIW"
     CDS             complement(4561..5709)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg243_7"
                     /sec_met_domain="Gly_radical (E-value: 6.2e-46, bitscore:
                     144.4, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.7e-111, bitscore:
                     362.3, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VLWFNNAPENWKRFCAKVSIDTSAIQYENDDLMRPDYGDDYGIAC
                     CVSPMKIGKQMQYFGARANLAKCLLYAINGGRDERSGVQVAPRFEPITSEYLDYNEVME
                     KYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMALHDGSVERIRATGIAGLSIVADSL
                     AAIRDTKVKVIRDERGLAVGFEREGDYVPFGNNDDRTDSIAVDITEKFMEYLRQHQTYR
                     NATPTQSILTITSNVVYGKKTGATPDGREGGTPFAPGANPMNGRDTKGAVAALASVAKL
                     PFQHAHDGISYTFAVSPATLGKERDIQIANMVGLLDGYFTPDGGQHLNVNVFDKELLLD
                     AMEHPENYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
ORIGIN
        1 ttccaaagga tcgagttcac gaagttcggt ctttgccgat tccggaagga tgccgagatc
       61 gatcagtttc gtccacagca acgatcctgc ggtgacacag caacgacccg aacggtggtc
      121 gaaataagtt ccgtcactca aaaagccacc tttttcgttg agtttcaggg ctgcactacg
      181 ttccggctcg ggtaattgct ccggaggcag aaggaagaga taaccgagtt tttccagttc
      241 ttcccgtacc cgcaggacag tatatccaca atccagttga ttctggagct ggtaaagatt
      301 gaaacaaatg tttcgggcta aaggagggat aggagaagcg gtggcaactg ttatcacaga
      361 actttccacc agactttcaa tttcatgcat cgacataatt gtatagatta gaatgaatct
      421 tgcaaagata gcaatacccg gatataaacc gacatagaaa cgcttctgaa tcgatttata
      481 cccgggtatt atttttatgc cttccggaaa gttctgcggc ttataacatt ccccgcatcc
      541 ggtcttcgaa agctgtcagg gctgctttgg ccccttcgcc catcgaaata atgatctgct
      601 tataagggac agtcgatacg tcgccggccg catagatacc tggaacgctt gtacgacaat
      661 ggctatcgat cacgatctca ccctgacggt tcgtatctac cacctcacgg aacggaccgc
      721 tgttggctgc caggccgatc tggacaaaga tgccatccag ttcgatgttg cggacttctt
      781 ctgtcttacg gtcctttata cggataccgg tcactttttc accgttaccc agtacctcca
      841 tcgtctggga atgcataaag atctccacgt tcggaaggct tcttgcctta tcctgcaaga
      901 cctgatccgc tttcaactca tcggcaaatt cgagaagcgt cacttttgaa cagataccag
      961 ccaggtcgat agccgcctca acgcctgaat ttcctccgcc aacaacagcc acatgcttgc
     1021 ctttatagaa cggaccgtcg caatgcggac agaaagcgac accgcgtccg atatattcgg
     1081 attctccggg gacattcaac ctacgccagc tggctccggt tgctacgata acggccgggg
     1141 ctatcagttt ttcgccggta gaggttgtca gcagcttttc gcgtccttcg acttccacct
     1201 tctcgatacg gcggtgttca aggatatcga tcggataatc tttcatatgc gacatcaggt
     1261 cgagagccag ctgctgcccc gtcgtctgcg aaacagaaat catattctcg ataccgacag
     1321 tttccttcac ctgtccgccg atacggtcgg ccacaacagc caccgccaaa cctttacggg
     1381 cagtatagat agctgccgca cttccggcag gacctccgcc gacgatgata acatcgtagt
     1441 tcttttctac caccgacagg ttctcctctt tcacgccata ctgcgcttcg agtttgccaa
     1501 gcagttcgcc aaagtctcca cgacctacat ggatcagttt accgtcggca aatacggaag
     1561 gtactccctg cactttcatg gcagcaacct cttcctggtt gatcgcaccg tccaccattt
     1621 cgtgagagat ttccgggttc agtgtagcca tggcattcaa agcctgtaca acgtcaggac
     1681 agttggtaca ggtcaaagaa acatatgtag tcagtttgac cgggccattc agggccttca
     1741 cacggttaca aatactatcg tcgggaatat ttttgccttt gccgtcactg ttcaggatag
     1801 ccagcagcaa cgaagtaaat tcgtgtccgt tgggaacgcc ccggaattta ataccggttt
     1861 tttccccgtt tttcagcagg gagaactcca ggccttcgcc gtctgccacc tgtacggtga
     1921 tcttatcgga acaggaagcc acatcgttca gcaattccaa tagttccttc ctgctttcat
     1981 gttcaggaga aacttgtata tcaaatatat attcgttcgt caatccgcta aagataccgg
     2041 acaactgatt tctcattgat gaatctaaca tagttgaatc ctttatttta attatgaata
     2101 gaacaactga tatttgttta gataaaaaag gccgacaata tgttgccggc ctccagtttt
     2161 actattagat cttaccgacc agatcgatgc tcggtttcaa tgtttcgcca ccttttttcc
     2221 atttggcggg gcaaacttcg ctcggatgtt cggcgatgaa ctgggcagct tctactttac
     2281 gaagcagttc gtcggcgtta cggccgatac cgttatcgtt gatttctgcc agtttgatct
     2341 ttccttccgg atttaccagg aatgtaccgc ggtaagccat accgtcttct tcgatcatta
     2401 caccgaatgc acggctcaaa gcgcctgtcg ggtctgccag catcgggtat ttgatcttac
     2461 gaatcgtttc agatgcatcg tgccatgctt tgtgtacgaa gtgtgaatct gtacttacag
     2521 aataaacttc tacacccatt gcctggaact ggtcatattt gtcagccata tctaccaact
     2581 cggtcggaca aacaaatgta aagtcagccg ggtagaaaaa gaaaatcgcc catttgcctt
     2641 tgatgtcgtc gctgcttaca gttttgaaac taccgttgtg gtatgcctga actttaaact
     2701 cgggtgcttg tgaattgata ataggttcca tagaattttt attttaatcg ttattactat
     2761 tttgtttttg ttactggtgc aaagataagt tgccgattcg aggtaagaac aaaaaatcca
     2821 atcgaaaggt tttatgcagt catagacaaa atctttgagt atcagtaaat caggtttata
     2881 atatacctgt ttcagtctga gcaacagtgc ccatcgcact tgaatcccga cgtttcaaat
     2941 tcgagcgtag catgctggat tcccacatgc tgaaggtcgt gttttatctc ctgtttgata
     3001 cgttccattt ctgaaatatc cgcaacggaa acatgggcgg taagtgccgt ttcggtcgta
     3061 ctgatcgccc agatatgaag atggtggaca ccttcgacgc cttcaaccga atgtatcgtc
     3121 ttttccacct tatcgatgtc cgttcccgcc ggcacaccgt ccagagagag acggatactg
     3181 tcgcgcagca gtccccaggt agaaacaatg ataacgatcg caatcaacag gccgataatc
     3241 gggtcgatga tataccatcc ggtatgggca ataacaattc cggagacaac cacgccgacc
     3301 gataccagtg catcggcagc catatgtaag taagcacctt tcacattcaa gtctttatcc
     3361 ttatccttca taaaaagcca tgccgtaagt gcatttataa cgactccgat acctgccacc
     3421 caagcgatcg catcaccctc gannnnnnnn nnaccgcatt cagaagggat acgagtacag
     3481 tgcttttctt atatccatag gtatagctgc aagtcgggtg caccttagcc agccggaaag
     3541 ccagcatagc cagtaccagg ctggctacat ctcccaggtt atgccctgcg tccgacaaaa
     3601 gtcctaatga attataccag aagccagccg taaattcggc tacgacaaaa gccaggttca
     3661 gggaaatacc tataatgaaa gccttgttca aagaggttat ttcgtggtgg tgatgatcgt
     3721 gatgatgttg ttccatattc attcctactt ttatttgagt aacaatatgg gacaaaacta
     3781 tgttttttag agcaataaaa atatacctga ttataggtaa attaccagat ggttataccg
     3841 tgcttcttaa atatttcttt ggcatttgcc agacgttcgg ccgataaagg ttctacctct
     3901 ttcagtttgt agtcgagacc ttgtgcctcg tatttataag cgcccatcgt gtgataaggc
     3961 aagagttccg ccttctgtac aaccgtatag tggctgatgt attcggccag tttatccaag
     4021 gcttcgtcat tatccgtcag tccgggcaca atgacatggc gtatccaaac cgggacaccg
     4081 cgtttttcca gttcgtccag gaaacgtaac gtattatcca gtttcacggc cgttagacgg
     4141 ggatgcaggt cgggatcgat ggtttttata tccaataaga ccaggtcggc atattccaga
     4201 accgccaggg ctttcggtgt gcagatatag ccggatgtat cgagcgccgt atgaataccg
     4261 gcttcccggc aaagacggaa aaactccctg agaaattcag cttgcaataa gggttctccc
     4321 ccggtgacag tcacgccacc cttggcgata aagttcttat atcgtaaaac ctcagccaat
     4381 aattcttcag gcgtcatctg atatttggca agccctttgc tatcccaggt atccggattg
     4441 tggcaataca ggcaacggag cggacacccc tgcataaaga caacaaaacg aattccggga
     4501 ccatcaacgg tcccgaaact ctctagtgaa tgtatgtttc ctttaatttc caactttcaa
     4561 ttacaaagaa tgattgattg ttcttgaaat cacatccagc tgctgttcgc gtgtcagttt
     4621 cacaaagttt actgcataac ccgatacacg gattgtcaac tgcggatagt tttccggatg
     4681 ctccatagca tcgagcaaca gctccttgtc gaatacgttc acattcagat gctgtccgcc
     4741 gtcaggagta aagtaaccgt ccagcaggcc gaccatattg gcgatctgaa tatcacgttc
     4801 ttttcctaac gttgcaggag aaacagcgaa tgtataagag ataccgtcgt gtgcatgctg
     4861 gaacggcagt ttagcaacag aagccagtgc agcaacagcg cctttggtat cacgtccgtt
     4921 catcgggttc gcacccggag caaacggcgt accaccttcg cgaccgtcgg gagttgcacc
     4981 tgtcttcttt ccgtatacga cattagacgt aatagtcagg atagactgtg tcggagttgc
     5041 attacggtat gtctggtgct gacgcagata ttccatgaac ttttcagtga tgtcgacagc
     5101 aatactatct gtacgatcgt cattgttacc gaaaggaaca taatcgcctt cacgttcgaa
     5161 accgacagcc aggccacgtt cgtcgcggat cacttttact ttcgtatcgc ggatagcagc
     5221 caatgagtcg gcaacgatag aaaggccggc aatacctgtt gcgcggatac gttctaccga
     5281 accgtcatgc aaagccatct cgaatgcttc atacgcatat ttatcgtgca tgtaatggat
     5341 gatcttcagt gcgttcacat aaactttagc cagccagcgc atcatctgtt cgtatttctc
     5401 catcacttcg ttgtaatcca ggtattcgct ggtgatcggt tcaaacctcg gagccacctg
     5461 tacacctgat ctttcatcgc gcccgccgtt gatggcatac agcaaacatt tagccaggtt
     5521 ggcacgtgcg ccgaaatact gcatctgctt accgatcttc atcggagaca cgcagcaggc
     5581 aataccataa tcatctccat aatccggacg catcagatcg tcgttctcat actggatagc
     5641 ggaagtatcg atagaaacct tcgcacagaa acgtttccag ttttcaggag cgttgttgaa
     5701 ccacagtac
//
