LOCUS       c00186_gnlPro..         5170 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|DIFEPDJN_186.
ACCESSION   c00186_gnlPro..
VERSION     c00186_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 06:39:48
            Original ID  :: gnl|Prokka|DIFEPDJN_186
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5170
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5170
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1749:5169]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1750..5169
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..5170
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5170
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             3..599
                     /locus_tag="ctg186_1"
                     /transl_table=11
                     /translation="NQPAASAIYPSITYTVADVAAQTDLVAAKATDATKASNASGVSLK
                     FGHILTQINFTVKAADALTYKLKTLTISGVHNKGTYGFDDSIWKSQEGTATYAHTIDDG
                     ALEVNTTGVEVNTAWMLMPQTLDTNAKINVVYDIYEKDVLLDTVTSAIDLNDTQAWGEG
                     KKIRYTLTLANKAAKVTFVPEVGPWSTADDTNVEK"
     CDS             664..1611
                     /locus_tag="ctg186_2"
                     /transl_table=11
                     /translation="MKVFGECFFYEYYLSIFVTATNKQIYLPMPNSSACRGKKQCAQCP
                     KAVDNAIIYASHLRGFHLPARKCEENIIIFLLKGEVLVNSKEYAGTVLHAGEFILQAIG
                     SKYEILAMTDVECVCYRFSKPEFFCEDRYNHIMKEVTPPLIFYPLTITPELQLFLESSK
                     AYLSEEKICREMLCFKRKELAFILGNYYSDYELSMLIHPLAQYTNSFHYFVLQNHAKVK
                     TVEELAQLGGYTVATFRRIFNSVFHQPVYEWMMERRKESVVYELRYTDASISEICYKYG
                     FESLPHFSNFCKKNFGASPRSLRAGNLSEVNGMS"
     CDS             complement(1750..2505)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer4_7"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg186_3"
                     /sec_met_domain="Fer4_7 (E-value: 4.3e-13, bitscore: 38.3,
                     seeds: 208, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_3 (E-value: 1.4e-26, bitscore: 81.2,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 1.6e-07, bitscore: 20.2,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKNISFTLKVWRQKGPKAKGAFETYQMKDIPGDTSFLEMLDILN
                     EQLISEGKEPVVFDHDCREGICGMCSLYINGHPHGPATGATTCQIYMRRFNDGDTITVE
                     PWRSAGFPVIKDLMVDRTAYDKIMQAGGYVSVRTGAPQDANAILIPKPIADEAMDAASC
                     IGCGACVAACKNGSAMLFVSAKVSQLNLLPQGKPEALRRAKAMLSKMDELGFGNCTNTR
                     ACEAECPKNVSISNIARLNRDFIIAKLKD"
     CDS             complement(2539..4485)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 199.7; E-value:
                     2.8e-61)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg186_4"
                     /sec_met_domain="FAD_binding_2 (E-value: 2.3e-62, bitscore:
                     199.7, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 3.9e-38,
                     bitscore: 118.8, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTKIDSKIPEGPVAEKWTNYKAHQKLVNPANKRRLDIIVVGTGLA
                     GASAAASLGEMGFRVFNFCIQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTVKG
                     GDYRAREANVYRLAEVSNNIIDQCVAQGVPFAREYGGTLDNRSFGGAQVSRTFYAKGQT
                     GQQLLLGAYSALSRQVNVGTVKLYTRYEMEDVVLIDGRARGIIAKNLVTGKLERFAAHA
                     VVIATGGYGNAYFLSTNAMACNCSAAMACYRKGAWFANPAYVQIHPTCIPVHGDKQSKL
                     TLMSESLRNDGRIWVPKKLEDAKKLQEGTLQGKDIPEEDRDYYLERRYPAFGNLVPRDV
                     ASRAAKERCDKGFGVNNTGLAVFLDFSEAINRLGKDVVAQRYGNLFDMYEEITDVSPYE
                     NPMMIYPAIHYTMGGIWVDYELMTSIKGLFAIGECNFSDHGANRLGASALMQGLADGYF
                     VLPYTIQNYLADQITVPRFSTDLPEFAAAEKAVQEKIDWMMNIKGKKSVDSIHKELGHI
                     MWEYVGMGRTAEGLKEGLKKLKEVRKEFEKELFIPGDKEGMNVELDKAIRLYDFITMGE
                     LVAYDALNRNESCGGHFREEYQTEEGEAKRDDENYFYVACWEYQGSDEKEPVLLKEPLV
                     YEAIKVQTRNYKS"
     CDS             complement(4522..5169)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: SucD_FumR"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg186_5"
                     /sec_met_domain="SucD_FumR (E-value: 2.5e-130, bitscore:
                     420.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="GIALVLFLTFHMAMNLVALVSANGYNMVCEFLGANWYALVATVGL
                     AALFIIHIIYAFWLTMQNRKARGSERYAVTEKPKTVEWASQNMLVLGIIVIVGLGLHLV
                     NFWAKMQLPELMHNMGMHADTLTLAYAANGVYHIQNTFSNPVFVVLYLVWLGALWFHLT
                     HGFWSSMQSLGWNNKVWINRWKCISNIYSTIVVLCFALVVVVFFAKSLCGAC"
ORIGIN
        1 acaatcaacc ggctgctagt gctatatatc cttccattac ttatacggta gcggatgtag
       61 ctgcccaaac ggacttggta gccgcgaaag cgacggatgc aaccaaggca tcaaatgcca
      121 gtggggtatc tttgaaattc ggccatatac tgacccaaat aaatttcact gtcaaggcag
      181 ccgacgcttt gacttataaa ctgaaaacac ttacaatatc cggtgtgcac aataaaggaa
      241 cttatggttt tgatgattca atatggaagt cacaggaagg aacagccact tatgcacata
      301 cgattgacga tggtgcattg gaagtgaata caacgggtgt ggaagtgaat acagcctgga
      361 tgttgatgcc ccagacgttg gatacgaatg ccaaaatcaa tgttgtctat gacatctatg
      421 agaaagacgt tcttcttgat actgtaactt ccgccattga cttgaatgat acacaagcct
      481 ggggtgaagg caaaaagata cgttatacgc tgacgctggc caataaggcg gcaaaagtaa
      541 cctttgtacc tgaagttggt ccatggagta cggctgatga tacgaacgtg gaaaaataaa
      601 acggcactgt ccgcaataaa aaactgaatt acttctattt tattcattcc ccgaaacttc
      661 tttatgaaag ttttcgggga atgtttcttt tatgagtatt atttatctat ttttgtcact
      721 gcaacaaaca aacaaatcta cttgccgatg cccaactctt ctgcttgcag aggtaaaaaa
      781 caatgcgctc aatgtcccaa ggctgtggac aatgcaataa tatatgcttc ccacctcaga
      841 ggattccatc tgccggcgcg aaagtgtgag gaaaatatta taattttcct gctgaaagga
      901 gaagtgcttg tcaacagtaa ggagtatgcg ggaactgtgt tgcatgcagg tgagttcatc
      961 cttcaagcta ttggttccaa gtacgaaata cttgccatga cggatgtgga atgcgtttgt
     1021 taccggttca gtaagcctga attcttctgt gaggaccggt ataaccatat tatgaaagag
     1081 gttacccctc cattgatatt ctatcctttg accattactc ccgagttgca gctctttctg
     1141 gaaagttcca aggcctattt gtccgaggag aagatttgcc gggaaatgct ttgtttcaaa
     1201 cggaaagaac ttgctttcat tcttggaaat tactactccg actatgagct ttccatgctg
     1261 atacatcctc ttgcccagta tactaattcc ttccactact ttgtcttgca gaatcatgcc
     1321 aaagtcaaga cggtagagga acttgcccag ttgggaggat atacagtagc tactttccgg
     1381 cgtatattca attctgtttt tcatcaaccg gtgtacgagt ggatgatgga acggaggaag
     1441 gaaagcgtgg tttatgaact tcgttatacg gatgcttcta tatcagagat ttgctataaa
     1501 tatggctttg aatcgctgcc tcatttttcc aatttctgca aaaagaattt cggggcttct
     1561 ccccgtagct tgcgtgccgg caatttatct gaagtaaacg gcatgtcata aaaaaactcc
     1621 tccggcggtt tcccggcaga ggagtctctt gtgatattaa tcaaatatag tgaaagctga
     1681 gtgataaatc tgagcttgct caaaaggttt atccgaagcg catcctatat tatttgcaga
     1741 aagaccggat tagtctttca gtttcgcaat gataaagtcg cggttcaggc gagcaatgtt
     1801 gctgatggaa acattcttcg ggcattcagc ttcgcacgca cgagtgtttg tacagttacc
     1861 gaaaccgagt tcgtccatct tgctcaacat agccttggca cggcgcaatg cttccggttt
     1921 gccttggggc aacaggttca gctggctcac cttggcagaa acgaacagca tagcagaacc
     1981 gttcttacaa gcagccacac aagcaccaca accgatacag ctggcagcat ccatagcctc
     2041 gtcagcgatg ggcttcggaa tcaggatagc gttggcatcc tggggagcac cggtacgtac
     2101 gctgacgtag ccgcctgcct gcataatctt gtcgtaggcc gtacggtcga ccatcaagtc
     2161 cttgataacc gggaaaccgg cagagcgcca cggttcaaca gtgatggtat cgccgtcgtt
     2221 gaaacgacgc atataaatct ggcaagtcgt agcgccggtt gcaggaccgt gggggtgtcc
     2281 gttgatgtag agcgaacaca taccgcagat accttcgcgg cagtcgtggt cgaatacaac
     2341 cggttcttta ccttcagaaa tcagctgttc gttcaagata tccagcattt cgaggaatga
     2401 agtatcgccc gggatatctt tcatttggta tgtttcaaaa gcgccttttg ctttcggacc
     2461 cttctggcgc cataccttga gcgtaaatga tatattttta tccattttct ttaattcttt
     2521 aaatgttcaa cttcccgatt agcttttgta gttacgagtc tgtaccttga ttgcttcgta
     2581 aaccagcggc tctttcagca ataccggttc cttttcgtca gaaccctgat attcccagca
     2641 agccacgtag aagtagtttt catcgtcacg cttggcttcg ccttcttcag tctgatattc
     2701 ttcacggaag tgaccgccgc agctttcgtt acggttcagt gcatcgtaag caaccagctc
     2761 gcccatagtg atgaagtcgt acagacggat agccttgtcg agttctacat tcataccttc
     2821 cttgtcgccc gggatgaaca gttctttctc gaactccttg cggacttctt tcagcttctt
     2881 cagaccttcc ttcaagcctt cggccgtacg tcccatacct acatattccc acataatgtg
     2941 gcccagttcc ttgtggatag agtctacaga cttcttaccc ttgatgttca tcatccagtc
     3001 aatcttctcc tgaacggcct tttctgcagc tgcaaattcg ggcaggtcgg tagagaaacg
     3061 gggaactgta atctggtcgg ccagatagtt ctggatagtg taaggcaata cgaagtaacc
     3121 atcggccaga ccttgcatca gggcggaagc accgaggcgg tttgcaccgt ggtcggagaa
     3181 gttacattca ccgatggcga acagtccctt gatggaggtc atcagttcgt agtcaaccca
     3241 gataccaccc atggtatagt ggatagccgg ataaatcatc atcgggtttt cgtatgggct
     3301 gacatcagtg atttcttcat acatatcgaa gaggttgccg taacgttgtg caacgacatc
     3361 tttacccaga cggttgatag cttcggagaa gtcaaggaat acggcaagac cggtgttgtt
     3421 cacgccgaag cccttgtcgc agcgttcttt ggcagcgcgg ctggcaacgt cacgcggaac
     3481 gaggttaccg aatgccggat aacggcgttc caggtagtag tcgcggtctt cctcgggaat
     3541 atcttttcct tgcagtgtac cttcctgcag tttcttggca tcttccagct tcttcggaac
     3601 ccagatacga ccatcgttac gcagagattc ggacatcaaa gtcagcttag actgcttgtc
     3661 accgtgaacc gggatacaag tggggtgaat ctgtacgtaa gcagggttgg caaaccaggc
     3721 acctttacgg tagcaagcca ttgctgcaga gcagttacat gccattgcat tggtagaaag
     3781 gaagtaggca ttgccgtaac caccggtagc gattaccaca gcgtgggcgg caaaacgttc
     3841 caatttaccg gtaacgaggt tctttgcaat gataccgcgg gcacgtccgt caatgaggac
     3901 aacgtcttcc atttcgtagc gggtatacag tttcacagtg cctacgttca cctggcggct
     3961 caatgcagag tatgcaccca gcaacagctg ctgtccggtc tgacccttgg catagaaagt
     4021 acgggatacc tgagcaccac cgaaagaacg gttgtccagt gtaccgccgt attcgcgggc
     4081 gaaaggaaca ccttgtgcaa cgcattggtc gataatattg ttggatactt cagccagacg
     4141 gtaaacgttg gcttcgcggg cacggtagtc gccacccttt actgtatcgt agaacagacg
     4201 gtatacagag tcaccgtcgt tttggtaatt cttagcagca ttgataccac cttgtgcagc
     4261 gatggagtgt gcacggcgcg gagagtcctg gatgcagaag ttgaatactc tgaaacccat
     4321 ttcgcccaga gaggcggcag cactggcacc ggcaagtccg gttcctacta cgatgatgtc
     4381 cagacgacgt ttgttggcag ggtttactaa tttttggtga gccttataat tggtccattt
     4441 ctcagccacc ggtccttccg gtattttaga atctatctta gtcataatgc aatttacaat
     4501 ttagtcattt acaatttgaa tttagcaagc gccgcacaat gattttgcaa agaatacgac
     4561 aactaccaga gcgaagcaga ggacaacgat agtagaatag atgttggaga tacatttcca
     4621 acggttaatc catactttgt tgttccagcc caaagactgc atggagctcc agaaaccgtg
     4681 agtgaggtgg aaccacaatg cgcccagcca aacgaggtaa agcactacga ataccggatt
     4741 gctgaaggtg ttctgaatgt gatagacacc gttggctgca tatgccagcg tgagggtgtc
     4801 agcatgcatg cccatgttgt gcatcaactc gggaagctgc attttagccc agaagtttac
     4861 caggtgcagg cccaaaccca cgattacgat gatacccagt acgagcatgt tttgagaagc
     4921 ccattccaca gtcttgggct tttccgtaac ggcatagcgt tcgctaccac gtgccttacg
     4981 gttctgcata gtcagccaga aagcgtagat gatgtggatg atgaataagg cagccagacc
     5041 taccgtagcc accaatgcgt accagtttgc tcccaggaac tcacaaacca tgttgtagcc
     5101 attagccgag actaatgcaa ccaggttcat cgccatgtga aatgtcagaa acaggacaag
     5161 ggcgataccg
//
