LOCUS       c00340_gnlPro..         2485 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|BGKMDACC_340.
ACCESSION   c00340_gnlPro..
VERSION     c00340_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 10:03:42
            Original ID  :: gnl|Prokka|BGKMDACC_340
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2485
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2485
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:1685]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2..1685
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..2485
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2485
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..889
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg340_1"
                     /sec_met_domain="Gly_radical (E-value: 2.6e-47, bitscore:
                     147.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 2.3e-61, bitscore:
                     195.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="APPSPRYTEEILNYDEVMALYDKMQDWLAKTYIDALNIIHYMHDK
                     YCYERIEMALHDPEILRTMATGIAGLSVAADSLSAIKYATVKAIRNEEGLIVDFKTEGE
                     FPCYGNNDPRVDDIACSLVSNFMEKLRRLHTYRNSLPTQSILTITSNVVYGKKTGNTPD
                     GRRAGEPFAPGANPMHGRDRNGAVASMLSVAKLSYDDSLDGISYTFSIVPQALGKEERE
                     RRVKLVSLLDAYFAATGHHINVNVLERETLLDAMDHPEKYPQLTIRVSGYAVNFIKLTR
                     EQQQEVINRTFHTR"
     CDS             906..1685
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 53.7;
                     E-value: 9e-17)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg340_2"
                     /sec_met_domain="Radical_SAM (E-value: 4.1e-32, bitscore:
                     99.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQVFPQNRAPDGESSVTGLVHSVESCGTVDGPGIRFVLFLSGCS
                     LRCRYCHNPDTSYVRRGRLRSADDVLKEIARYRDFLQAAGGGVTLSGGDPLFQPDFAGA
                     VLKGCRKLGLHTCLDTSGHLGVNAGEEMLADTDLVLLDIKAWNPKRYRALTGGELHPTL
                     EFAERLASLRKPVWLRYVLVPGVTDNLEEIEELSRHAALLGNVERVDVLPFHQLGRFKW
                     DELKLDYTLKDVPEASAELAEQVRGIFRRGGFPDCVH"
     CDS             complement(1933..2484)
                     /locus_tag="ctg340_3"
                     /transl_table=11
                     /translation="LYLQLGGLHGLDVATPSLFPVKKAAPAEDAPILIAPFSRLGSASE
                     WSWEQWRELVSLLPGRTVLAALEEDRERAASLAEHLNVELAVGAPEALFPVMDVAAAAV
                     AVDGDFPSLCSFRGLPVVTLFSTRLPDVYRPMGTFNRSLYSHQCCSPCFRKECDRDVPC
                     NRHIAVRDVLEALREISGKE"
ORIGIN
        1 ggccccgccc tccccccgct acacggagga aattctcaac tatgatgaag tgatggccct
       61 gtacgacaag atgcaggact ggctcgccaa aacttacatt gacgctctga acatcatcca
      121 ctacatgcac gacaaatact gctatgaacg cattgaaatg gcgctgcatg atccggaaat
      181 tctgcgcacg atggctacgg gaatcgccgg gctttccgtg gcggcggact ccctgtccgc
      241 catcaagtat gccaccgtaa aagccatccg caatgaagaa gggctgattg tggacttcaa
      301 gacggaaggg gaattcccct gttacgggaa caatgacccg cgcgtggacg acatagcatg
      361 cagcctggtc agcaatttca tggaaaaact gcgcaggctc cacacttacc gcaattccct
      421 gcccacccag tccatcctga cgatcacctc caatgtggtg tacggcaaaa agacgggcaa
      481 cacgccggac ggccgccgag ctggggaacc gttcgccccc ggagccaacc ccatgcacgg
      541 aagggacagg aacggagccg tggcctccat gctctccgta gccaagctgt cctatgacga
      601 ctccctggac ggcatctcct acaccttctc catcgttcct caggccctgg gcaaggaaga
      661 acgtgaacgc cgcgtcaagc tcgtctccct gctggacgcc tactttgccg ctacaggtca
      721 ccacattaat gtgaacgtac tggaacggga aacgcttctc gacgccatgg atcacccgga
      781 aaaatacccg cagcttacca ttcgcgtttc cggctatgcc gtgaatttca tcaagctgac
      841 ccgggaacag cagcaggagg tcatcaaccg cactttccac acccgttaac ccggactgca
      901 agcgaatgcc gcaggttttt ccacaaaacc gtgctccgga cggagaaagc tccgtcaccg
      961 gtttggttca ttccgtggaa tcctgcggca ccgtggacgg ccccggcatc cgcttcgtcc
     1021 ttttcctgtc cggctgcagc ctgcgctgcc gctactgcca taatccggat acctcctacg
     1081 tgcggagggg ccggctcagg agcgcagatg atgtcctcaa ggaaatagcc cgttaccggg
     1141 attttttgca ggcggcgggc ggcggagtta ccctctccgg cggggatccc ctcttccagc
     1201 cggattttgc cggtgccgtg ctgaaaggct gccggaaact gggcctgcac acctgcctgg
     1261 acacctccgg ccatctgggc gtgaatgcgg gcgaagaaat gctggcggat acggacctgg
     1321 tcctgctgga tatcaaggca tggaatccga aacggtatcg cgccctgaca ggcggggaac
     1381 tgcaccctac actggagttt gcggaacgcc tggcctccct ccgcaagccc gtgtggctga
     1441 ggtatgtgct ggtacctggc gttacggaca atctggaaga aattgaggaa ctctcccgcc
     1501 atgcggcgct tctgggcaac gtagaacgcg tggacgtgct ccctttccac cagctgggga
     1561 gattcaaatg ggatgaattg aagctggact atactttgaa ggacgtcccg gaagcgtccg
     1621 cagaactggc ggaacaggtt cgcggcattt tccggcgggg cggttttccg gattgcgtgc
     1681 attaaccgga ttttcgcgct ttggcgccac caaccgcagc aaaccggacg caggcatccc
     1741 cctcttccgg atgaggggcc ctcttccctc cactacagaa tccatgcaaa aaggaagacg
     1801 catttctgga attgctccgg aatacggcat ttccgcaatt cctttcgtta ttccatcatt
     1861 ctttacggca acggcagcat tcctgccgtt tttccgccaa cgccgggggg cggcattccg
     1921 tcccttccca gattattctt tcccggaaat ttcccgcaag gcttccagta cgtcccgcac
     1981 ggcaatgtgg cggttgcagg gaacatcccg gtcacattcc ttccggaagc agggagaaca
     2041 gcactggtgg ctgtaaaggg aacggttgaa tgtgcccata gggcggtaaa catccggaag
     2101 ccgggtggaa aacagagtca ccaccggaag cccccggaaa gaacagaggg acggaaagtc
     2161 gccatccaca gctactgcag ccgcagcgac atccatgacg ggaaagagag cctccggcgc
     2221 acccaccgcc agctccacat tcaaatgttc cgccagggag gcggcacgct cccggtcttc
     2281 ttccaaagcc gccagcaccg tccgtcccgg cagaagggaa accagctccc tccactgctc
     2341 ccagctccat tcactggcgc tccccaggcg ggaaaacgga gcgattaaaa tgggggcgtc
     2401 ttccgccggg gccgcttttt tcacagggaa caaagacggg gtggcgacgt ccagcccatg
     2461 cagccccccc aattgcagat aaagc
//
