LOCUS       c00378_gnlPro..         4491 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|HELNOICE_378.
ACCESSION   c00378_gnlPro..
VERSION     c00378_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 04:57:14
            Original ID  :: gnl|Prokka|HELNOICE_378
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4491
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4491
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:2479]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2..2479
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..4491
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4491
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             2..1696
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 147; E-value:
                     2.8e-45)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg378_1"
                     /sec_met_domain="FAD_binding_2 (E-value: 1.8e-46, bitscore:
                     147.0, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 2.4e-39,
                     bitscore: 122.5, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="AKNYQNDGDSVYRLFYDTIKGGDYRAREANVYRLAEVSNSIIDQC
                     VAQGVPFAREYGGLLDNRSFGGAQVSRTFYARGQTGQQLLLGAYSALSRMIGLNKVKMY
                     TRHEMLDVVKVDGRARGIIARNLITGQIERFSAHAVVVATGGYVNTFFLSTNAMASNGS
                     AAWQCYKKGAYFANPCMVQIHPTCVPVKGDYQSKLTLMSESLRNDGRIWVPKKLEDAKA
                     LQAGTKKGKDIPEADRDYYLERRYPAFGNLVPRDVASRAAKERCDAGFGVNNTGLAVFL
                     DFSDAINRLGKDVVKAKYGNLFDMYEEITNDDPYETPMMIYPALHYSMGGLWVDYELMT
                     SIPGLFAIGEANFSDHGANRLGASALMQGLADGYFVLPYTIQNYLSDQIQVPRFSTDLP
                     EFAEAEKGIKDRIKKLMSIKGKESVDSIHKKLGHIMWEHVGMARDAKGLQEAIQMLKEL
                     KKEFWSNVYIPGEADNLNVELDKALRLADFIEIGTLMAHDALDRAESCGGHFRTEHQTE
                     EGEALRHDDKFMYVSCWEYQGEDKDPVMLKEDLNYQFVVPQTRNYKK"
     CDS             1724..2479
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg378_2"
                     /sec_met_domain="Fer2_3 (E-value: 2.7e-27, bitscore: 83.2,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 8.9e-13, bitscore: 36.8,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 9.5e-07, bitscore: 17.5,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKDIKVTLKVWRQKSPKDKGQFETYQIDKINQSVSFLEMLDILN
                     ERLVSEGKEPIVFDHDCREGICGMCSLYVNGHPHGPATGATTCQLYMRRFNDGETITIE
                     PWRSAGFPVIRDLMVDRYAYDKIIQAGGFVSVNTGGVPDANSIAIPRADAEMAMDAAAC
                     IGCGACAAACKNGSAMLFVSAKVSQLALLPQGKVEAARRAKAMVAKMDELGFGNCTNTR
                     ACEMECPKNISVSNIARLNREFICAKLQD"
     CDS             complement(2778..3623)
                     /locus_tag="ctg378_3"
                     /transl_table=11
                     /translation="MEDNIPKFDLPVDFIVGNSITGEILNQYGRFPCKIKAGIFVLCVQ
                     GTVHATVNLTEFTIRKNDFITLPPGCFIQIHEVSDDVKLCFAGFSSVFVSHINYIKTIT
                     NYLPVIFDSPIMPLPEHISLLYQQAFDLLINAYSMPKTIENKEIIKAIFTIFMQGVIEL
                     YRNHTPYSNAPMTRNMEICREFVQLAMENYTKEHSVSYYAKRLNITLQHFCYVIKKVSG
                     RTALDILSNIIIMDAKAQLKSTDMPVKKIAFALGFDNLSFFNKYFRQHVGMTPQEYRES
                     "
     CDS             3864..4490
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Radical_SAM"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg378_4"
                     /sec_met_domain="Radical_SAM (E-value: 9.1e-16, bitscore:
                     46.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTKRTKSSFRTMSTNRKLESVFLFVTGRCNAKCKMCFYAEDMDK
                     KEKDLTFEEIKTISETAGQFNRLWVSGGEPTLREELPEIIEMFYKNNEIKDVNIPTNGL
                     KPDRIVAWVKQIRQNCPELNINLSLSLDGFGDTHDIQRGVPGNFYKAMGTLKLIEKNFA
                     DDGRVLKNLATVITKYNENEVEDLMIWMLGRFHLSNHIIEAARGMT"
ORIGIN
        1 tgcaaagaat tatcagaatg atggtgactc tgtttaccgt ctgttctacg atacaatcaa
       61 aggtggtgac tatcgtgccc gcgaagcaaa cgtttatcgt ctggctgaag tttccaactc
      121 tattatcgac cagtgcgtag cacagggtgt tcctttcgct cgcgaatacg gtggtttgct
      181 ggataaccgt tcattcggtg gtgctcaggt atcccgtacg ttctatgccc gcggtcagac
      241 cggacagcag ttgttgctgg gtgcttactc tgctttgagc cgtatgatcg gtttgaacaa
      301 agtaaagatg tatacccgcc acgaaatgct ggacgttgtg aaggtggatg gccgtgcccg
      361 cggtatcatc gcacgtaacc tgatcacagg tcagatcgaa cgtttttctg ctcacgcagt
      421 agtggtggca acaggtggtt atgtaaatac tttcttcctg tctactaacg caatggcttc
      481 caacggttct gctgcatggc agtgctacaa gaaaggtgct tacttcgcta acccttgtat
      541 ggtgcagatc cacccgactt gtgtaccggt gaaaggtgac taccagtcta agctgacttt
      601 gatgtctgaa tcattgcgta acgacggtcg tatctgggtt ccgaagaaac tggaagatgc
      661 aaaagcatta caggccggaa ctaagaaagg gaaagacatt cctgaagcag accgcgacta
      721 ctatctggaa cgccgttatc cggcattcgg taacctggtt ccgcgtgacg tggcttcacg
      781 tgccgctaaa gaacgttgcg acgccggctt cggtgtgaac aacacaggtc tggccgtgtt
      841 cctcgacttc tccgacgcca tcaaccgtct gggtaaagat gttgtaaaag ctaaatatgg
      901 taacttgttc gacatgtacg aagaaattac caacgacgat ccatacgaaa ctccgatgat
      961 gatttatcct gcattgcact actcgatggg tggtctgtgg gttgactatg aattgatgac
     1021 ttctattccg ggtctgttcg ctatcggtga agctaacttc tctgaccacg gagctaaccg
     1081 tctgggtgct tctgcgttga tgcagggatt ggccgatggc tactttgtat tgccttatac
     1141 aattcagaac tatctgtcag accagatcca ggttccacgt ttcagcaccg acctgcctga
     1201 gtttgcagaa gctgaaaagg ggatcaaaga ccgcatcaag aaattgatga gtatcaaggg
     1261 taaagagtcg gtagattcta tccataagaa actgggtcat atcatgtggg aacatgtagg
     1321 tatggcacgt gatgcaaaag gtttgcagga agcgatccag atgttgaaag agctgaagaa
     1381 agagttctgg agtaatgtat atattccggg tgaagccgat aacctgaacg tagagttgga
     1441 taaggcattg cgtttggctg actttattga gattggtaca ttgatggcac acgatgcatt
     1501 ggaccgtgcc gaatcttgtg gaggacactt ccgtacagag caccagaccg aggaaggcga
     1561 agccctgcgt catgacgata agttcatgta cgtatcttgt tgggaatacc agggtgaaga
     1621 caaagatccg gttatgctga aggaagattt gaactatcag ttcgttgttc ctcaaacacg
     1681 taactacaag aagtaataat catcaatcac aaaaattaga atcatggata aagatataaa
     1741 agtaacactt aaagtttggc gtcagaaaag tccgaaagat aaaggacaat ttgagactta
     1801 ccaaatagac aaaatcaatc agagtgtttc tttcctggaa atgttagaca ttttgaatga
     1861 acgtttggtc agtgaaggta aagagccgat cgtattcgat cacgactgtc gtgaaggtat
     1921 ctgcggtatg tgttctttgt atgtaaacgg acatccgcac ggacctgcaa ccggcgcaac
     1981 tacttgccag ctttatatgc gtcgcttcaa tgatggcgaa actattacaa tcgaaccgtg
     2041 gcgttctgcc ggtttcccgg taatccgcga cttgatggta gaccgttatg cttacgataa
     2101 aattatccag gcaggtggtt tcgtatcagt gaacacaggt ggtgtacctg atgccaattc
     2161 aatcgctatt cctcgtgcag atgctgaaat ggctatggat gctgctgcat gtatcggttg
     2221 cggtgcttgt gctgctgctt gtaagaacgg ttcggctatg ttgttcgttt ctgcaaaggt
     2281 tagccagttg gctctgttgc ctcagggtaa ggtagaagct gctcgtcgtg cgaaagcaat
     2341 ggttgccaag atggacgaac tgggcttcgg taactgtacc aacacgcgtg cttgcgaaat
     2401 ggaatgtccg aagaacatct ctgtcagcaa catcgctcgt ttgaaccgcg agttcatttg
     2461 tgctaaattg caagactaat atacattata tatgtatgtc cggagaggcg ttgcttatgg
     2521 cagcgcctct tcttttttgc ccctatttta acatttctga agtcatagat aaatatcttg
     2581 aatgttgtta gtgtttctta tatgctatac aacactaatt agcattttaa ctattgacaa
     2641 gttggagaat acctgtatct ttgtactgtg gttttttcat aatagtatta gatttaaggt
     2701 taacaaagtt ggttagggct tgtcgtgaga caggctttaa ttgtttatag gggtttgcgt
     2761 ccggcatatc ccttttttta gctctccctg tattcctgcg gggtcattcc tacatgttgc
     2821 cggaaatatt tgttgaagaa cgaaaggttg tcgaaaccca gggcgaatgc aattttcttg
     2881 accggcatat cggtcgattt aagctgcgct ttggcatcca taatgatgat attgctcagg
     2941 atatccaatg cagtccgtcc gctgaccttt tttatcacat aacagaaatg ctgcaaggtg
     3001 atgttgagcc ttttggcgta ataagacacg ctatgctctt tcgtgtagtt ttccatcgcc
     3061 agttgtacga actcccggca tatttccatg ttacgcgtca tgggagcatt gctatatggc
     3121 gtatgattcc tgtataattc aataacccct tgcatgaaga tagtaaagat tgccttgata
     3181 atctccttgt tctcgatggt tttaggcatg gaataagcat tgatcagcaa gtcgaaagcc
     3241 tgttggtaga gtaaggatat atgttccggc agaggcatga tcggactgtc gaatatgaca
     3301 ggcagatagt tcgtaatcgt ctttatataa ttgatatggc tgacgaatac ggaagaaaag
     3361 cctgcaaaac acagttttac atcatcggaa acctcatgta tctgaatgaa gcaacccggg
     3421 ggtaaggtga taaagtcgtt cttccggatg gtaaactcag ttaagtttac agtcgcgtgg
     3481 actgtccctt gtacgcaaag cacgaatatg ccagccttga tcttgcacgg gaaacgtccg
     3541 tactggttca gtatttcacc tgtaatactg tttcctacaa taaaatctac ggggaggtcg
     3601 aatttaggaa tattgtcttc catactttta tatacactta attgaatgta caaagatagt
     3661 ttttttgaga caagttagtc gataatctta aatatagaac atcgaatccc acaaatggag
     3721 attgttttgt gctgtataat agttgaaatt tgcgaatgtt ttcaagtctt gctgatagat
     3781 ttgttgggat ggtatatatt tgtctatctg ctaaataaga tatgggagct aatactaata
     3841 gagaaatcta atcgataata acaatgagta cgaaaagaac aaagagctct ttcaggacaa
     3901 tgagcacaaa cagaaaatta gaatctgttt tcctgtttgt cacaggacgg tgtaatgcta
     3961 aatgtaaaat gtgtttctat gcagaagaca tggataaaaa ggagaaagac ctgacttttg
     4021 aagaaatcaa gacaatctcc gaaacagccg ggcagttcaa ccgtttgtgg gtatccggcg
     4081 gtgaaccgac actgcgtgag gagttacctg aaattataga gatgttctac aagaataacg
     4141 agataaagga cgtgaatatc cctaccaacg gattgaagcc tgaccggatc gtcgcctggg
     4201 taaaacagat ccgccagaat tgtccggaac tgaatatcaa cctgagcctt tctttggacg
     4261 gtttcggtga tacgcatgat atacagcgcg gcgttcccgg caatttctac aaagcgatgg
     4321 ggacactgaa gctgattgaa aaaaactttg ctgacgacgg cagggtgttg aaaaacctgg
     4381 ccaccgtaat caccaaatat aacgagaatg aggtcgagga tctgatgata tggatgctgg
     4441 gacgtttcca tctttccaat catattattg aagctgcacg cggtatgacc c
//
