LOCUS       c00330_gnlPro..         2458 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|EFMMBJOO_330.
ACCESSION   c00330_gnlPro..
VERSION     c00330_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 05:23:08
            Original ID  :: gnl|Prokka|EFMMBJOO_330
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2458
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2458
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[98:2457]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      99..2457
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..2458
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2458
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(99..878)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 54.5;
                     E-value: 4.9e-17)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg330_1"
                     /sec_met_domain="Radical_SAM (E-value: 2.8e-32, bitscore:
                     99.5, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQVFPQNHDPDGESSVTGLVHSVESCGTVDGPGIRFVLFLSGCS
                     LRCRYCHNPDTSYVRRGQLRSADDVLKEIARYRDFLQAAGGGVTLSGGDPLFQPDFAGA
                     VLKGCRKLGLHTCLDTSGHLGVNAGEEMLADTDLVLLDIKAWNPKRYRALTGGELRPTL
                     EFAERLASLRKPVWLRYVLVPGVTDNLEEIEELSRHAALLGNVERVDVLPFHQLGRFKW
                     DELKLDYTLKDVPEASAELAEQVRGIFRRGGFPDCVH"
     CDS             complement(895..2457)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg330_2"
                     /sec_met_domain="Gly_radical (E-value: 5e-47, bitscore:
                     146.1, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.1e-189, bitscore:
                     618.7, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LAQLGASYGCDLTRPAANAREAVQWTYLGYLAAVKEQNGAAMSLG
                     RVSTFFDIYFTRDLEQRLITEEEVQEIIDQFVMKLRIVRFIRTPDYNNLFSGDPTWVTE
                     SIGGMGEDGRTLVTRSSFRMLQTLYNLGPAPEPNLTVLWSRNLPEAFKSFCAKVSIETS
                     SVQYENDDLMRPHWGDDYGIACCVSAMRIGKQMQFFGARANLAKCLLYALNGGVDELKG
                     KQVAPPSPRYTEEILNYDEVMALYDKMQDWLAKTYIDALNIIHYMHDKYCYERIEMALH
                     DPEILRTMATGIAGLSVAADSLSAIKYATVKAIRNEEGLIVDFKTEGEFPCYGNNDPRV
                     DDIACSLVSNFMEKLRRLHTYRNSLPTQSILTITSNVVYGKKTGNTPDGRRAGEPFAPG
                     ANPMHGRDRNGAVASMLSVAKLSYDDSLDGISYTFSIVPQALGKEERERRVKLVSLLDA
                     YFAATGHHINVNVLERETLLDAMDHPEKYPQLTIRVSGYAVNFIKLTREQQQEVINRTF
                     HTR"
ORIGIN
        1 ggattctgca gtggagggga gagggcccct catccggaag agggggatgc ctgcgtccgg
       61 tttgctgcgg ttggtggcgc caaagcgcga aaatccggtt aatgcacgca atccggaaag
      121 cctccccgcc ggaaaatgcc gcggacctgt tccgccagtt ctgcggacgc ttccgggacg
      181 tccttcaaag tatagtccag cttcaattca tcccatttga atctccccag ctggtggaaa
      241 gggagcacgt ccacgcgttc cacgttgccc agaagcgccg catggcggga gagttcctca
      301 atttcttcca gattgtccgt aacgccaggt accagcacat acctcagcca cacgggcttg
      361 cggagggagg ccaggcgttc cgcaaactcc agtgtggggc gcagttctcc gcctgtcagg
      421 gcgcgatacc gtttcggatt ccatgccttg atatccagca ggaccaggtc cgtatccgcc
      481 agcatttctt cgcccgcatt cacgcccaga tggccggagg tgtccaggca ggtgtgcagg
      541 cccagtttcc ggcagccttt cagcacggca ccggcaaaat ccggctggaa gaggggatcc
      601 ccgccggaga gggtgactcc gccgcccgcc gcctgcaaaa aatcccggta acgggctatt
      661 tccttgagga catcatctgc gctcctgagc tggcccctcc gcacgtagga ggtatccgga
      721 ttatggcagt agcggcaacg caggctgcag ccggacagga aaaggacgaa gcggataccg
      781 gggccgtcca cggtgccgca ggattccacg gaatgaacca aaccggtgac ggagctttct
      841 ccgtccggat catggttttg tggaaaaacc tgcggcatcc gcttgcagtc cgggttaacg
      901 ggtgtggaaa gtgcggttga tgacctcctg ctgctgttcc cgggtcagct tgatgaaatt
      961 cacggcatag ccggaaacgc ggatggtaag ctgcgggtat ttttccgggt gatccatggc
     1021 gtcgagaagc gtttcccgtt ccagtacgtt cacattaatg tggtgacctg tagcggcaaa
     1081 gtaggcgtcc agcagggaga cgagcttgac gcggcgttca cgttcctcct tgcccaaggc
     1141 ctgaggaacg atggagaagg tgtaggagat gccgtccagg gagtcgtcat aggacagctt
     1201 ggctacggag agcatggagg ccacggctcc gttcctgtcc cttccgtgca tggggttggc
     1261 tccgggggcg aacggttccc cggctcggcg gccgtccggc gtgttgcccg tctttttgcc
     1321 gtacaccacg ttggaggtga tcgtcaggat ggactgggtg ggcagggaat tgcggtaagt
     1381 gtggagcctg cgcagttttt ccatgaaatt gctgaccagg ctgcatgcta tgtcgtccac
     1441 gcgcgggtca ttgttcccgt aacaggggaa ttccccttcc gtcttgaagt ccacaatcag
     1501 cccttcttca ttgcggatgg cttttacggt ggcatacttg atggcggaca gggagtccgc
     1561 cgccacggaa agcccggcga ttcccgtagc catcgtgcgc agaatttccg gatcatgcag
     1621 cgccatttca atgcgttcat agcagtattt gtcgtgcatg tagtggatga tgttcagcgc
     1681 gtcaatgtaa gttttggcga gccagtcctg catcttgtcg tacagggcca tcacctcatc
     1741 atagttgaga atttcctccg tgtagcgggg ggagggcggg gccacctgct tgcccttgag
     1801 ttcatccacg ccgccgttca gggcgtagag aaggcacttg gcgaggttgg cgcgcgctcc
     1861 gaagaactgc atctgcttgc cgatgcgcat ggcggacacg cagcatgcga tgccgtagtc
     1921 gtcgccccaa tgcgggcgca tcaggtcgtc gttctcgtat tggacggagg acgtttcaat
     1981 ggaaaccttg gcgcagaagc ttttgaaggc ttccggcagg ttccgggacc acagaacggt
     2041 caggttcggt tccggagccg gccccaggtt gtacagggtc tgcagcatgc ggaaggaact
     2101 gcgggtgacg agggtgcgcc cgtcctctcc catgccgccg atggattccg tcacccaggt
     2161 ggggtctccg gagaacaggt tattgtaatc aggcgtgcgg atgaaacgga cgatgcgcag
     2221 cttcatcacg aactggtcga tgatttcctg aacttcctct tccgtgatga gcctctgttc
     2281 caggtcgcgg gtgaaataaa tgtcgaagaa agtggaaacg cggcccaggg acatggccgc
     2341 tccgttctgt tccttcacgg cggccaggta gcccaggtac gtccattgca cggcttcgcg
     2401 ggcgtttgcg gcggggcgag tgaggtcgca gccgtaggag gcgcccagct gggcgagt
//
