LOCUS       gnlProkkaKELFPOHK_33   11663 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KELFPOHK_33.
ACCESSION   gnlProkkaKELFPOHK_33
VERSION     gnlProkkaKELFPOHK_33
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-12-01 22:33:18
            Original ID  :: gnl|Prokka|KELFPOHK_33
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 11663
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..11663
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[3094:6663]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3095..6663
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     cand_cluster    1..11663
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..11663
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="phosphonate"
                     /region_number="1"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     CDS             complement(36..1109)
                     /NRPS_PKS="Domain: NAD_binding_4 (10-144). E-value:
                     4.5e-07. Score: 21.1. Matches aSDomain:
                     nrpspksdomains_ctg33_1_NAD_binding_4.1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score:
                     105.1; E-value: 7.9e-32)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg33_1"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 1.6e-108,
                     bitscore: 353.6, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="RmlD_sub_bind (E-value: 4.3e-08, bitscore:
                     23.9, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVFKDKVLLITGGTGSFGNAVLRRFLDSDIKEIRIFSRDEKKQD
                     DMRHHLQNPKVKFYIGNVRDRQSVDGAMMGVDYVFAAAALKQVPSCEFFPLQAVQTNVI
                     GTDNVLESAIAHGVKNVVVLSTDKAAYPINAMGISKAMMEKVAIAKGRALGEEAGTTIC
                     CTRYGNVMASRGSVIPLWVEQIKNGRPITVTDPNMTRFMMTLDDAVDLVIYAFEHGHNG
                     DLFVQKAPAATLDVLAQALKETYAQVDPKYGETEVKTIGTRHGEKLYETLVTREEMAKA
                     VDMGDYYRIPCDTRDLNYDKFFTEGNEDIARIEDYHSHNTARLDVEGMKKLLLKLRFIR
                     EDLGMEEKAKSAEIKSE"
     aSDomain        complement(678..1079)
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg33_1_NAD_binding_4.1"
                     /evalue="4.50E-07"
                     /label="ctg33_1_NAD_binding_4.1"
                     /locus_tag="ctg33_1"
                     /protein_end="144"
                     /protein_start="10"
                     /score="21.1"
                     /tool="antismash"
                     /translation="ITGGTGSFGNAVLRRFLDSDIKEIRIFSRDEKKQDDMRHHLQNPK
                     VKFYIGNVRDRQSVDGAMMGVDYVFAAAALKQVPSCEFFPLQAVQTNVIGTDNVLESAI
                     AHGVKNVVVLSTDKAAYPINAMGISKAMME"
     CDS_motif       complement(1026..1082)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg33_1_0001"
                     /evalue="1.70E-03"
                     /label="PKSI-KR_m1"
                     /locus_tag="ctg33_1"
                     /protein_end="28"
                     /protein_start="9"
                     /score="11.1"
                     /tool="antismash"
                     /translation="LITGGTGSFGNAVLRRFLD"
     CDS             complement(1124..1801)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg33_2"
                     /sec_met_domain="Glycos_transf_1 (E-value: 5.3e-13,
                     bitscore: 40.3, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAFWTENPYISEKVKHLFPTIKAYTVSNYYNQVFDDENTQIEHA
                     LPSFDGCTLLSINAPYPHKNLAIAIEVARYLKKHYSEFSFRFVFTVTENDLPPIPSYLR
                     EHFCLIDKVSVAECPSLYRQADIMFQPTLLECFTATYVEAMRMQVPILTTDLPFAHGLC
                     GNAAVYYSPLDAEDCAKSIYKMAVDTSFQDYLVGEGIKQLKSFDNYNRRAEKLIDLISN
                     LDD"
     CDS             complement(2298..2387)
                     /locus_tag="ctg33_3"
                     /transl_table=11
                     /translation="MKTNFNIRSIIVINKIIINFFFRQYRWYL"
     CDS             complement(2394..3110)
                     /locus_tag="ctg33_4"
                     /transl_table=11
                     /translation="MSRGISEKEQRWLLVLSQVIEVLDKNNLLYFIDTGTLLGAVRDKM
                     FIPWDNDIDLAVIGGDTSTTNLLKVSKLLYALGFYVTLTKKEIAICSRDGVNLGIKLYK
                     KQASHYEACMGKVYRHRLFYSINLYLSGEVLYKKGFSFKYKAISLFVKFSQVFKLKLSC
                     IWQMKLLFWAKYDEKTVIIPFEFVDAIIDYNFYNIIVKVPNKPERYLAYRYGENWKVPK
                     QDYDYMIDDKSIINID"
     CDS             complement(3095..4189)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Fe-ADH"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg33_5"
                     /sec_met_domain="Fe-ADH (E-value: 4.9e-76, bitscore: 247.2,
                     seeds: 218, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEQRFFLGESSIKELIPILQEYTPDHIFLVRGKKSYEKCGAQAVL
                     ESVFVKLACGVTEFFDFEANPKIEDMEKGLSLLKEYPTNMIIAIGGGSVLDMAKLLRFF
                     YSYSGNPTGGEFEKKRQLIPLITLPTTAGTGSEATHFSVLYKDKVKYSVEHDAILPDVA
                     IVYPKFTYGNSKYLTSCTGFDALAQAIEAYWNVNATEESDKYAAKAIKLLWPNLPLVVN
                     SPTAEARNKVSEGSYWAGRAINITKTTAPHAFSYSFTTDYGYPHGHAVALTFPFFMRYN
                     IGNSDILLNPNINPYLYKIKMQKMLQWLNMECVDVAIAMKEYIRNLGLDIDISQIDISK
                     IVSSVNIQRLINNPYKVEAICLEE"
     CDS             complement(4197..5327)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055:pyruvate oxidase/decarboxylase (Score: 69;
                     E-value: 6e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg33_6"
                     /sec_met_domain="TPP_enzyme_N (E-value: 5.9e-17, bitscore:
                     53.2, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 9.7e-21, bitscore:
                     65.5, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MISPNIFVDSLGAMQMDFFTGVPDSLLKNICAYLTDHLASEHNII
                     AANEGAAIGLATGYYLATRKIPVVYMQNSGLGNAVNPLMSLTDKEVYNIPLLLLIGWRG
                     EPGVKDEPQHIKQGKVTLPLLEAMGIRYAVLSQDEKKFISQLNVAKSYMDETREPFAFV
                     IQKDTFLEYSSENCEDERLSLKREAAIHFIAASLTEKDIIISTTGMISRELFEYRESTS
                     LEHERDFLTVGSMGHASQIALGIALKKRDCKVYCFDGDGSTLMHMGSLAIVGSLHPENY
                     VHVVFNNGAHDSVGGQPTVALDIDLCKIAQACGYEIVLSVSDNEGLCEALKKIQQIRGP
                     ILLEVKVRKGARKNLGRPANTPIQNKEAFMLFLDNL"
     CDS             complement(5359..6663)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: CTP_transf_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231:carboxyvinyl-carboxyphosphonate (Score: 202.6;
                     E-value: 1.3e-61)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg33_7"
                     /sec_met_domain="CTP_transf_like (E-value: 1e-12, bitscore:
                     39.8, seeds: 65, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 2.7e-119, bitscore:
                     388.9, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 8.9e-64, bitscore:
                     206.6, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKKKVYVGMSADIIHPGHLNIIHEASKLGHVTVGVLTDSAIASY
                     KRLPYLNFDQRSLIVLNLKGVDEVIPQTTLDYVPNLELIRPDFVVHGDDWKEGIQQETR
                     QRVIDTISKWGGKVIDVPYTKGISSTLLNTRLKEIGTTPEIRLKRLKRLIAAKSIVRIC
                     ESHSGLTGLIIENTSVEVNGVKREFDGMWSSSLTDSTSKGKPDIEAVDLTTRLHDLNDA
                     LECTTKPVIFDGDTGGKIEHFVFTVRTLERLGISAVIIEDKIGLKKNSLFGTDAVQTQD
                     TIEGFCDKIQAGKRAQITDDFMIIARIESFIAGKGLDDALGRALAYIKAGADGIMIHSK
                     DKSGEDIKNFCLALRKIHKSIPIVVVPTTYNHISEDELASWGVNVVIYANHMLRSAYPA
                     MLNTAKSILANARSYEANDLCMPIKEILELIPGTK"
     CDS             complement(6626..7756)
                     /locus_tag="ctg33_8"
                     /transl_table=11
                     /translation="MKVTTDNSFYNLVIGNNKMILLLMLACIVTYYYAMKFALVIELYG
                     AGEGRMMRFEAGELFTSNLEVYLYDFLIKPFTQFVMLIVACMLLSKIKRRILLLSVLFL
                     FLYASVGNGRGGLIVFLFYCFIIYYLRRELYYINLLASKRKKWNFGVVSFVCLFVGVGI
                     IVVVISYMTAQRMGFDTFSAEVVKIGFEELKSQAVWYFLGPFRALDYSFRANYESITGF
                     WYGRATMGGIDNLLKLVIHYLGGSYTIAHDEIGHLLQNSKISVGNGIIFNYAYTNIIFH
                     YLDFGYVGVLLFPFLYGFIVRYSIYKFTQKPTLLTLGIMIYLVYISMFNIFSWEFQKPS
                     SIIYVLLLLFFQYVYSHKFVILKLNGKKKGLCRHEC"
     CDS             complement(8002..8934)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg33_9"
                     /sec_met_domain="Glycos_transf_2 (E-value: 2.3e-25,
                     bitscore: 80.8, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNGSITVSICCLTYNHEKYIRQCLDGLIMQKTEFDFEILVHDDAS
                     SDATQSIINEYVVAYPNLIKPILQIRNQYSQGISPLRQILFPLVKGKYVALCEGDDYWT
                     DPLKLQKQVNAMESDHSLAGCAHQSLVFYENGTPSHMFHHVKKQILKTKDLLDVRLFHT
                     ASFLFKKEVLDYIKDFNSSLYAGDRFLFLACSLKGNILYMPDVMCCYRKNAMGVTSRVS
                     YSLHVLDLKVPEYFHNKDLKFPYYRYLAFVHRAIFSESLDISLKKYIYHFTLFVKYSFS
                     YFPKNLKDIYKALYVSFWWLTKGQKYRIK"
     CDS             complement(8935..9930)
                     /NRPS_PKS="Domain: NAD_binding_4 (91-233). E-value: 3e-07.
                     Score: 21.7. Matches aSDomain:
                     nrpspksdomains_ctg33_10_NAD_binding_4.1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score:
                     114.5; E-value: 1.1e-34)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg33_10"
                     /transl_table=11
                     /translation="MKKKIIIFGATGNTGSYLLEYALNFFNKEEYEIIASGRRETTFFS
                     KRGIPYYSVDITKEEDFDKLPMENIHAVMLLSAQIPSYMDTYCPIKYLDSIVIGGFNVL
                     EYCRKCHVDRIIYTQTVFDISLYSHDTILKPDMKPNFSYTGDHAIYVISKNTALDLIEH
                     YYQEYGLKKFIFRLPTIYSYSPYPYYFPNGVKTKRPIYQMIENAQKGLPIEIWGDPHYA
                     KDMVHVNDFSQMLCKAVEVEQESGFYNVGTGMPITLEEQIRTIISVFSPKESPSEVIYR
                     PDKVCGGGYIMNIENAKKELGYVPQYDCRKLFEDYKSEMEIKRFAELRLK"
     aSDomain        complement(9232..9657)
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg33_10_NAD_binding_4.1"
                     /evalue="3.00E-07"
                     /label="ctg33_10_NAD_binding_4.1"
                     /locus_tag="ctg33_10"
                     /protein_end="233"
                     /protein_start="91"
                     /score="21.7"
                     /tool="antismash"
                     /translation="YLDSIVIGGFNVLEYCRKCHVDRIIYTQTVFDISLYSHDTILKPD
                     MKPNFSYTGDHAIYVISKNTALDLIEHYYQEYGLKKFIFRLPTIYSYSPYPYYFPNGVK
                     TKRPIYQMIENAQKGLPIEIWGDPHYAKDMVHVNDFSQ"
     CDS             complement(9927..10892)
                     /locus_tag="ctg33_11"
                     /transl_table=11
                     /translation="MMSIGGYFELEIREGEHFHQGAIKLSTARQCFEYILKARRYTKVY
                     IPYYTCDVIIEPLMKLNVKYEYYHINSLLDPVFDKKIESSEAFLYTNYFGLKQDTVLYL
                     SRFYPNLIVDNAQAFFAKPIEGVDTFYSARKFFGVPDGAYLYTTALLDIDFPIYNPIDN
                     INYLIGRITDSAEGYYKDFQESEERMSNQEIHRMSVLSDKILSSLDYVSIIERRRFNYH
                     LLYSALTAFNKFDFSISENQNVPLIYPFYTSKAGLRQQLILEHVYVPQYWQNVVNKIDA
                     KCLEYSIANNMIALPIDQRYDEKTMNILIEKISNLNNHLS"
     CDS             complement(10889..11578)
                     /locus_tag="ctg33_12"
                     /transl_table=11
                     /translation="MKLGIMQPYFMPYIGYFQLIKAVDKYVVYDDVNYIKGGWVNRNNI
                     LVNGEKKMFTITLKGASAYKHFNEIEIGDDFRKFMKTLELNYSKAPFYHETMELLYRII
                     MFPNRQLALFLANSFKEILDYLNIQTEILLSSDLPKDCSLKGKDKVLHICKLLSADTYY
                     NAIGGKQIYNKEDFMQNGINLKFVDTIIKRYPQQSKEFVPYLSIIDVLMNNSKEETCML
                     LDSFSLQ"
ORIGIN
        1 gattggatag gatggaaaag ttaacaatgg ctatactatt cgctcttgat ttccgcgctc
       61 ttcgcctttt cctccatccc gaggtcttcg cggatgaagc ggagtttcaa aagaagcttt
      121 ttcatgccct ccacatccag gcgggcggtg ttatggctgt gatagtcctc tatacgggca
      181 atatcctcat tgccttccgt gaagaacttg tcatagttca ggtcacgggt gtcgcagggg
      241 atgcggtagt agtcgcccat gtcgacagcc tttgccatct cctcgcgggt taccagtgtc
      301 tcatacagtt tctcgccgtg gcgcgtgccg atggtcttca cctccgtttc accgtatttc
      361 ggatcaacct gcgcgtatgt ttccttcaag gcctgcgcca gtacatccaa ggtagcggca
      421 ggagccttct gcacgaacag gtcaccgtta tgcccgtgct caaacgcata gattaccaga
      481 tccacggcat cgtccagcgt catcatgaag cgggtcatgt tggggtccgt taccgtaatg
      541 ggcctgccgt tctttatctg ctccacccac aagggaatta cagaaccgcg gcttgccatc
      601 acattgccgt aacgggtaca gcagatggtc gtaccggctt cctcacccaa cgcacggccc
      661 ttggctatgg caaccttctc catcatggcc ttgctgatac ccatggcatt gatgggatag
      721 gcggccttgt cggtagaaag caccaccaca ttctttacac catgcgctat ggcagattcc
      781 agtacattgt ccgtaccgat cacattggtc tgcacagcct gcaacgggaa gaactcgcaa
      841 ctgggaacct gcttcaaggc ggcagcggca aatacataat ccactcccat catggcaccg
      901 tctacagact ggcggtcacg gacattacca atgtaaaatt ttactttggg attttgcagg
      961 tgatgacgca tgtcatcctg ctttttctcg tcacgactaa aaatacgaat ctccttgata
     1021 tcagaatcaa ggaaacgacg caatacagcg ttaccaaaac tgccggtacc tccggttata
     1081 aggaggactt tgtctttaaa tactgacata aatataatat gtattaatca tccaaattcg
     1141 atatcaaatc gattaatttt tctgctctcc gattataatt atcgaaggac ttaagttgtt
     1201 ttattccttc tccgaccaga taatcttgaa aggatgtgtc tacagccatt ttataaattg
     1261 atttggcaca gtcttctgca tctaacggcg aatagtacac agctgcattt ccacataatc
     1321 catgagcaaa tggcaaatct gtagttaaaa ttggcacttg cattcgcatc gcttcgacat
     1381 atgtggcagt gaaacattca agtaaagttg gttgaaacat tatatctgcc tgacgatata
     1441 aggaagggca ttctgctaca ctaactttat caatcagaca gaaatgctct cgcaaataag
     1501 aaggaatagg tggcaagtca ttctctgtca ccgtaaaaac gaatcgaaaa ctaaattcgg
     1561 aataatgttt tttaagatat cgagcgactt cgatagcaat tgctaaattc ttatgaggat
     1621 aaggagcatt tatggacagt aaagtacaac cgtcaaaaga cggtaaggca tgttctattt
     1681 gagtattttc atcatcgaaa acctgattat aataattcga tacagtatag gcttttatgg
     1741 tagggaacaa gtgctttact ttctctgaaa tataaggatt ctcagtccaa aaagcagtca
     1801 caccacgttt gaagtaataa tataaataaa gatttctcgc cttttctatc attagttcct
     1861 tccaagacat acgttgaaaa tagggagaat cgttcaaaac gagatgggat ctggcaaatc
     1921 cacatagatg cttacatttc ggcttccatc ttgcaggtcc gaatatggta agaactccac
     1981 caatattgtg tctggttacc aaattatcca tagccttatc acgtccccaa aacaatgtat
     2041 accaactgtt tcgtatatca tgacgcacta cagtgacatt tttgtattcc gataccctat
     2101 cagcagtatc atccatacat gaggataata ctaaaaaaaa atcatgttgg ggaattgtat
     2161 tcaaacaaga acaaaccgaa tcagcgactt gaagccctcc acctttcttg agattggagc
     2221 aatttattag aatgttcatt tgttccgaga atataatata taagtgccca attttggtgt
     2281 ataaatgaaa gtgctattta taaataccat ctatattgtc taaagaaaaa gttgattata
     2341 attttattaa ttacaataat actccttatg ttgaaatttg ttttcatatt tttttaatca
     2401 atatttataa tagacttgtc atcaatcata taatcatagt cttgtttggg aaccttccaa
     2461 ttttcaccat atctatatgc tagatatctt tctggcttat ttggcacttt cacaattata
     2521 ttataaaaat tataatcaat aattgcatct acaaactcaa aaggaataat caccgtcttt
     2581 tcatcatact tagcccaaaa caataacttc atttgccaaa tacaagacaa ttttaactta
     2641 aagacttgac taaacttcac aaagagtgag atagctttat acttaaaaga aaagcctttt
     2701 ttatacagga cctctccaga gaggtataaa tttatagaat aaaatagtct atgtctataa
     2761 acttttccca tacatgcttc ataatgactt gcttgctttt tatataattt aattcctaaa
     2821 tttaccccgt ctctagaaca gatagcaatt tctttctttg tcaaagtgac gtaaaagcct
     2881 aaagcatata acaattttga cactttaagc aaattagtag tagatgtatc tcctccaata
     2941 actgctaaat caatatcatt atcccaagga ataaacattt tatctctaac agctcctaaa
     3001 agagtaccag tatcaataaa atataataaa ttatttttat caagaacttc aataacttgt
     3061 gataatacta acaaccatcg ttgctccttt tcacttattc ctctagacat atcgcttcaa
     3121 ctttatatgg attgtttatt aatctttgaa tattaacaga agatacgatt ttcgatatgt
     3181 caatttgaga tatgtcaata tctaatccca agtttcttat atactctttc atagcgattg
     3241 caacatctac gcattccata tttaaccact gtaacatttt ttgcatttta atcttatata
     3301 agtaaggatt tatattagga ttcaataaga tatccgaatt tccaatatta tatctcataa
     3361 aaaagggaaa tgttaatgct actgcatgtc catgcggata tccataatca gttgtaaatg
     3421 aatacgaaaa agcatgtgga gctgtagtct tagttatatt aattgcacga ccagcccaat
     3481 aagagccttc cgaaacctta ttcctagcct ccgctgtagg agaatttaca accaatggca
     3541 aattaggcca taagagttta attgctttcg ccgcatattt atccgactct tccgtagcat
     3601 tcacattcca ataagcttct atcgcctgcg ctagagcatc aaagcctgta caagatgtta
     3661 gatattttga atttccataa gtaaattttg gatatacaat cgctacatcc ggaagaatag
     3721 catcatgttc tactgaatat tttactttat ctttatataa aacagaaaaa tgagttgctt
     3781 cactacctgt accagctgta gtaggaagtg ttattaatgg aattaattgt cgcttctttt
     3841 caaactcccc ccccgtagga ttaccagaat aggaataaaa aaaacgaagc aattttgcca
     3901 tatctaatac actacctcca cctatagcaa taatcatatt tgtcggatat tctttcaata
     3961 atgaaagtcc tttttccata tcttcaattt taggatttgc ttcaaaatca aagaattccg
     4021 tcacgccaca agctaattta acaaaaacag attctaacac agcctgtgca ccacatttct
     4081 cataagactt ttttcctctt acaagaaata tatgatcagg agtatattct tgcaagatag
     4141 gaatcaattc ttttatagaa gactctccta aaaaaaaacg ctgttccata attactctat
     4201 aaattatcta agaaaagcat gaatgcctct ttattttgta taggagtgtt ggctggtctt
     4261 cccaaattct tacgagctcc ctttcgaacc tttacttcca acaaaatagg gccgcgtatt
     4321 tgctgtatct ttttaagagc ttcacataaa ccttcgttat cagaaacaga caatactatt
     4381 tcataaccac aagcttgagc aattttacat aaatcaatat ctaaagcgac tgttggttgt
     4441 ccccccacag aatcgtgagc gccattatta aagactacgt gtacatagtt ttccggatgt
     4501 aaagaaccga caattgctaa cgaacccata tgcatcaaag tggaaccatc tccatcaaag
     4561 caataaactt tacagtctct ctttttaaga gctattccca atgctatctg cgacgcgtgc
     4621 cccatagaac ctacagtcaa aaaatctctc tcatgttcta aagaagtaga ttcacgatat
     4681 tcaaataatt cacgtgatat catacctgtt gtcgatatta taatatcttt ttctgttaat
     4741 gaagctgcta tgaaatgaat agcagcttcg cgtttcaaag acaaccgttc atcttcacaa
     4801 ttttcagaac tatactctaa gaaagtgtct ttctgaatta caaaagcaaa aggctctctt
     4861 gtctcatcca tgtaagattt tgcaacatta agttgagata taaacttctt ttcatcttga
     4921 gataaaacag catatctgat acccattgcc tcaagtaatg gaagtgtgac ttttccctgc
     4981 ttaatatgtt gtggctcatc tttcacacca ggttcaccac gccaaccaat tagcaataac
     5041 aatggaatat tataaacttc tttatccgtc aatgacatta aaggatttac tgcatttcca
     5101 agacctgaat tctgcatata aacaactgga attttcctcg tagccaaata atatcctgta
     5161 gccaaaccta tagcagcacc ttcattagcg gcaataatgt tatgttcaga agcaaggtga
     5221 tctgttaaat atgcacatat attcttcaat aaagaatcgg gaactccagt aaaaaaatcc
     5281 atctgcatag cgccaagaga gtctacaaaa atattaggag aaatcataat aataagaatt
     5341 taattttaaa gagtctattt acttagtccc tggaatcaat tccagaattt ctttaatagg
     5401 catgcataag tcattggctt cataagagcg tgcattggca agaatggact tcgccgtatt
     5461 aagcatagcg ggatatgcac ttctaagcat atgatttgca tatattacaa catttactcc
     5521 ccaagaagcc aattcatctt ctgatatatg gttataagta gttggaacaa ccactatcgg
     5581 aatggattta tgtattttgc gtaaagcaag acagaagttc tttatatcct caccagactt
     5641 atctttgcta tggatcataa tcccatccgc acctgcttta atatatgcta atgcacgtcc
     5701 taaagcatca tctaaacctt ttccagcaat aaaactttca atacgagcta ttatcataaa
     5761 atcatccgtt atctgagcac gtttacccgc ttgaatttta tcacaaaacc cttcaattgt
     5821 atcctgggtc tgaacagcgt ctgttccaaa aagcgagttc ttcttcagac ctattttatc
     5881 ctcgataata acagctgata tccctaaacg ttccagcgtg cgaacagtaa atacaaaatg
     5941 ttctatttta ccacctgtat caccatcaaa aattactggt ttggtagtac actccaaagc
     6001 atcattcaaa tcatgaagac gagttgtcaa gtcaacggct tcaatatcag gcttaccttt
     6061 agaagtcgaa tcagtaagac tactggacca catcccatca aattcacgct taactccgtt
     6121 gacttctaca ctggtatttt ctattatcaa acctgttaac ccactatgag attcacagat
     6181 acgaacaata cttttagcag caattaagcg tttaagtcgc ttcaaacgaa tttcgggagt
     6241 ggttcctatt tccttcaacc tagtattcaa taaagtggaa gaaatacctt tcgtataagg
     6301 aacatctatc acttttccac cccatttaga gatagtatca atcacacgct ggcgagtttc
     6361 ttgttgaata ccctctttcc agtcatcacc atgtactaca aagtcaggcc taatcaattc
     6421 caaattcgga acataatcaa gagtagtttg aggtattact tcatcaacac ccttcaaatt
     6481 cagaactatc aatgagcgct gatcaaaatt caagtaaggc aaacgcttgt aactggcaat
     6541 agctgaatct gttaatacac caaccgttac atgccccaat tttgaagcct catgaatgat
     6601 attcaaatga cctggatgaa taatatcagc actcatgccg acataaacct tttttttttc
     6661 catttaattt taatattacg aatttatgag aataaacata ttgaaaaaat agcaacaaca
     6721 aaacataaat aattgaggaa ggcttctgaa attcccacga aaaaatattg aacattgata
     6781 tatagaccaa atatatcata ataccaagag tcagtaatgt tggtttttga gtaaatttat
     6841 aaatactata tcgtacgata aagccatata aaaatgggaa taaaagaacg ccaacgtaac
     6901 caaaatccaa ataatgaaat attatattag tgtatgcata attaaagatg attccattcc
     6961 caactgaaat ttttgaattt tgcaaaagat gacctatttc atcatgagca atggtataac
     7021 ttcctcctaa ataatggatt acaagtttta ataaattatc aattccaccc attgttgccc
     7081 ttccatacca aaacccggtt atcgattcat aattagctct aaatgaatag tctaaagctc
     7141 taaatggacc taaaaaatac catacggcct gactttttag ttcttcaaaa cctattttta
     7201 cgacctcagc actaaatgta tcaaatccca ttctttgagc tgtcatatat gaaataacca
     7261 caactattat tccaacgcca acgaacaagc atacaaaaga aactacacca aaattccatt
     7321 ttttcctttt gctggctaac aaattgatat agtacaattc tcttcttaaa taataaatga
     7381 taaaacaata aaatagaaaa acaatcaatc ccccacgccc attaccaaca gatgcgtata
     7441 aaaacagaaa aagcaccgat agcaacagaa ttctacgttt aatcttagaa agtagcatac
     7501 acgctacaat tagcataaca aactgtgtaa aaggttttat tagaaaatca tatagatata
     7561 cttctaaatt actcgtaaaa agttcaccag cctcaaatct catcatccga ccttcgcccg
     7621 ctccatatag ttcaataact agagcaaact tcattgcgta ataataagta acaatacatg
     7681 ccaacataag aagtaatatc atcttattat tgccaataac cagattataa aaactattat
     7741 ctgttgtaac cttcaatcgt atttttttta tttttccaaa acaaagaaat ccccaattaa
     7801 acgcccaaac attgatcaat aataacatat atgttttcat tgacacagaa tatagttcag
     7861 ctggatcaaa agtagaaata acaatagcag ttccccacca caataaatat atgaaactta
     7921 tttttcttga aaaagaatca attataagcc tattagatat caaaatctga attaagaata
     7981 gaataagaaa aattatagca atcatttgat tctatacttt tgtccctttg tcaaccacca
     8041 gaaagaaaca tataaagcct tatagatatc tttcagattc ttaggaaaat aagaaaaaga
     8101 atatttcaca aataaagtaa aatgatatat atatttcttt aaggaaatgt caagtgattc
     8161 tgaaaatata gctctatgaa caaatgccaa atatctgtaa taaggaaatt tcaaatcttt
     8221 attatgaaaa tattcaggaa cttttaggtc taatacatgt aatgaataag aaactctaga
     8281 agtaactccc attgcattct tcctataaca acacattaca tctggcatat ataaaatatt
     8341 tcctttcaac gaacaagcca aaaacagaaa acgatctcca gcatataaac tactattgaa
     8401 atctttaata taatcaagta cttctttttt aaaaaggaaa gacgctgtat gaaaaagtct
     8461 tacatccaat aagtctttag tttttaaaat ctgttttttc acatgatgaa acatatggga
     8521 tggagttcca ttctcataaa aaactaaaga ttgatgcgca caacctgcta acgaatgatc
     8581 actctccatt gcatttactt gtttttgtaa cttcaacgga tcagtccaat aatcatcccc
     8641 ctcacaaagc gcaacatatt taccttttac taaaggaaat aatatttgac gtaacggaga
     8701 aataccttga gaatactggt ttcgaatctg aagaattggt ttaataagat taggatatgc
     8761 cacaacatat tcattaataa tactctgagt agcatcagaa gacgcatcat catgaacaag
     8821 aatttcaaaa tcaaattctg ttttttgcat tataaggcca tctaaacact gccttatata
     8881 tttttcatga ttatacgtca agcaacatat gctaactgtt attgaaccgt tcatttattt
     8941 taatcttaat tcagcaaaac gctttatttc catttcactt ttataatcct caaacaactt
     9001 acgacaatca tattgaggca catatcctaa ttctttcttt gcattttcaa tgttcattat
     9061 gtacccacca ccacaaactt tgtctggtct atatataact tcagatggtg actccttagg
     9121 cgaaaaaaca ctaataatag tgcgaatttg ttcttctaat gtaattggca ttcctgtacc
     9181 cacattataa aagccacttt cttgttccac ctcaacagct ttacacaaca tttgactaaa
     9241 atcgttaaca tgaaccatat cctttgcata atgaggatct ccccatatct ctataggtag
     9301 tcctttctga gcattttcaa tcatttggta aataggtcgc ttcgtcttga ctccattagg
     9361 aaagtagtac ggataagggc tatagctata tattgttgga agcctaaaaa tgaatttttt
     9421 caaaccgtat tcttgataat aatgctctat caagtctaaa gcagtatttt tggaaattac
     9481 atatatagca tgatcacccg tatagctaaa atttggtttc atatcgggtt ttaaaatagt
     9541 atcatgagaa tacaatgaaa tatcaaaaac ggtttgagta taaattatcc tatctacatg
     9601 acatttgcgg caatactcta atacattaaa tcctcctatt acaatagaat ctaaatactt
     9661 tatggggcaa taagtatcca tataagaagg tatttgagca gagagtagca taactgcatg
     9721 tatattttcc attggaagct tatcaaaatc ttcttccttc gtaatgtcca cagaataata
     9781 tggtattccc cttttggaaa aaaaagtagt ttcgcgacgt cctgaagcta ttatttcata
     9841 ctcttcctta ttaaagaaat ttaatgcgta ttctaacaaa taagaccctg tattaccggt
     9901 tgctccaaaa ataataattt tctttttcat gataaatggt tatttaagtt agatattttt
     9961 tcaattaata tattcattgt tttttcatca tatctctgat ctataggcaa tgcaatcata
    10021 ttattcgcga ttgagtattc cagacattta gcgtctatct tattcactac gttttgccaa
    10081 tattgaggta catatacgtg ctctaatatt aattgttggc gaagcccagc tttgctcgta
    10141 taaaaaggat atatcaacgg gacattttga ttttcagaaa tactaaaatc aaatttatta
    10201 aatgcagtta atgcactgta gagaagatga taattaaaac gacgcctttc aattattgag
    10261 acgtaatcca aagaagacaa aattttatca gacaatacag acattctatg tatctcttga
    10321 tttgacatcc tttcttcaga ttcttgaaaa tctttataat acccctctgc agagtcagtt
    10381 atacgcccta tcaagtagtt aatattatca ataggattat aaatcgggaa atcaatatcc
    10441 agtaacgcag ttgtgtacaa ataagcgcca tctggaactc caaaaaattt acgggcagaa
    10501 taaaatgtat ctaccccttc aattggtttt gcaaaaaaag cttgtgcatt atcaactatt
    10561 aagttaggat aaaaccgaga taaataaaga acagtatctt gttttaagcc gaaataatta
    10621 gtatatagaa aagcctctga gctttctatt tttttatcaa acacaggatc cagtaaacta
    10681 tttatatgat aatattcata ctttacattt aatttcatga gaggttctat tatcacatca
    10741 caagtgtaat atggaatata cacttttgta taccgtctag cttttaatat atactcaaag
    10801 cattgacgag cagtactcag ttttattgca ccttgatgaa aatgttctcc ttcacgtatt
    10861 tccagttcaa aatagccacc tattgacatc attgtaaact aaatgaatct aataacatac
    10921 aagtttcttc tttagaatta ttcattagta catctattat tgataaatat ggaacaaatt
    10981 ctttagattg ctggggatac ctttttatta tcgtatctac aaatttcaaa ttaataccat
    11041 tttgcataaa gtcttcctta ttatagattt gttttccacc tatagcatta taataagtat
    11101 ctgccgatag taacttacaa atatgaagaa ctttatcctt tccttttaaa gagcaatctt
    11161 taggtaaatc tgaagatagc aatatttcag tctgaatatt tagataatct aaaatttctt
    11221 taaaactatt agccaagaag agagccaatt gtctatttgg aaacataatt atcctataca
    11281 acaattccat tgtttcatga tagaaaggag ctttagagta attcaattcc aatgttttca
    11341 taaatttacg aaaatcatct cctatctcta tttcattgaa atgtttatag gcactagccc
    11401 ccttcaaagt gatagtaaac attttctttt caccatttac caatatattg ttacgattca
    11461 cccaccctcc tttaatataa ttaacatcat cgtatacaac gtatttatca acagccttaa
    11521 ttagctggaa ataacctata taaggcataa aataaggttg cattatacct aatttcattt
    11581 caatatcaga tttaaaattc tttcaacatc ttcattcgac aatccacaat acattggcaa
    11641 gcagattaca ctttttgcca ttt
//
