LOCUS       c00222_gnlPro..         5207 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|FGCCJLKM_222.
ACCESSION   c00222_gnlPro..
VERSION     c00222_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 05:22:00
            Original ID  :: gnl|Prokka|FGCCJLKM_222
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5207
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..1800
                     /locus_tag="ctg222_1"
                     /transl_table=11
                     /translation="VQTCALPILYDVNKKVFNPLYGLTVAKKNMPMEAVLDILHPQDRA
                     PLIRLFSQLIGNEIEQGQMTLRFYNEQEEMYRYYESRMRLSTEHRGKLQIIGTQMDITE
                     RLQMAKKAQDLIAKRDLAMKVSNIVHWDFDVNSQKFESYNDPINDYVSDRLLTVSEYMD
                     VIYPEDRSVFYDAMQSLIAGKNVTINFTCRIQTKYDETWQYCDFMGVPFDQDENGDIIR
                     YTGFRQNIPKIQQLNRELKERNYKIELSFKTVGMSYWDFDVKSLKFSAFNDPINDFYSE
                     KTITPEDYLNATHPDDVVFVRKYMEHMFRGIDKDFNVKYRSKTKWDDEWQTLLVTGIQV
                     ERDKEGHVTRYTGITINNTKWEKMIQQLKELKEKAELSDRLKSAFLANMSHEIRTPLNA
                     IVGFSELMVTCDDPEEKKEYINIIQSNNELLLRLINDILDLSKIESGIIERRKENFNLA
                     KVCNELFVTIQAKMTNPNVELRLDGPNSECWVLLDRNRLKQVWMNFLTNAVKCTKSGYI
                     KMGYGIERKGLRIYVEDTGIGIPDDLHDKVFTRFEKLNEFSQGTGLGLTISRAIVEAAG
                     GEVGFTSKLGVGSTFWAWLPLRP"
     protocluster    1..5207
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1970:4946]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1971..4946
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5207
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5207
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             1971..4211
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg222_2"
                     /sec_met_domain="Gly_radical (E-value: 1.2e-46, bitscore:
                     145.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.7e-282, bitscore:
                     925.8, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENECWDHFKGETWKREINVRDFIQSNYTPYEGDDSFLVASSE
                     KTRRVWNKLTEMFKVEREKGVYDAETKLPQGIDVYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTEEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTAILIEEKKRFLNRLDIQEITEEIIQSREEISEQIKAL
                     KAFEKMCASYGFDVTRPAQNAREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEK
                     DIREGKLTEEEAQELVDQLIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTRTSYRYLHTLYNLGPAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRG
                     EYLEYDEVMAKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFM
                     EYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGA
                     IAALASVAKLPFQHAHDGISYTFAVSPATLGKEREVQINNLVSLLDGYFTPDGGQHLNV
                     NVFDKDLLIDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             4212..4946
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 67.6;
                     E-value: 4.7e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg222_3"
                     /sec_met_domain="Radical_SAM (E-value: 3.8e-34, bitscore:
                     106.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWI
                     RHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPL
                     SKERLDNAKAIFSRYKPENNKA"
ORIGIN
        1 gttcagacgt gtgctcttcc gatcttatat gatgtgaata aaaaggtttt taatccatta
       61 tatggactaa ccgtagccaa aaaaaatatg ccaatggagg cggtactgga tattttgcat
      121 ccccaagatc gagctccgtt aatccggctc ttctctcaat tgataggaaa tgagatagaa
      181 caaggacaaa tgactttgcg tttctataac gagcaagaag agatgtatcg ttattatgag
      241 agccggatgc gcctatcgac agaacatcgt ggaaaattgc agatcatcgg gacacaaatg
      301 gatataaccg aaaggttgca aatggccaag aaagcgcagg atctgatcgc aaaacgtgat
      361 ctggccatga aagtcagtaa tattgttcat tgggattttg atgtaaattc ccagaaattc
      421 gaatcttata atgatccgat caacgattac gtttcagatc gattattgac cgtttcggag
      481 tatatggatg ttatataccc cgaggaccgg tctgtttttt acgacgcaat gcaatccctg
      541 atagctggaa agaatgtcac catcaatttt acctgtcgca tacaaaccaa gtatgatgag
      601 acttggcagt attgtgattt tatgggtgtc cctttcgatc aagatgaaaa tggagatata
      661 atacgctata cgggattcag gcaaaacatt cctaagatcc agcaattgaa tcgggaactt
      721 aaagaaagaa attacaagat agagctttct ttcaagacgg ttggtatgtc gtattgggat
      781 tttgatgtaa aaagcctgaa attcagtgct tttaatgatc ctattaatga tttttattcg
      841 gaaaagacaa tcactcccga ggattatttg aacgctacac atccggatga tgtggttttt
      901 gtgcggaaat atatggagca tatgttccgg ggcatagata aagatttcaa cgttaaatat
      961 cgttccaaaa ccaaatggga tgacgagtgg cagaccttat tggttactgg aatccaggtt
     1021 gaaagagata aagaaggcca tgtgactcgc tataccggta ttacgatcaa taatacgaag
     1081 tgggagaaga tgattcagca attgaaggaa ttaaaggaga aagcggaact ttcggatcgt
     1141 ctcaagtctg cgtttctcgc taatatgagt cacgagatac gtaccccgtt aaacgctatt
     1201 gttggttttt cagagttaat ggtaacttgt gatgatccgg aagaaaaaaa agaatatata
     1261 aatatcatcc aatccaataa tgagttgtta ttgcgattga tcaacgatat ccttgatctt
     1321 tccaagatag agtcaggcat aattgaacgt aggaaggaga atttcaatct ggctaaggtt
     1381 tgtaatgagt tatttgttac gattcaggcg aagatgacaa atccgaatgt cgagttacgt
     1441 ttagatggtc ccaactcgga gtgctgggta cttctggata gaaaccggct taagcaagta
     1501 tggatgaatt tcctcactaa tgcggtaaaa tgcaccaaat ccggatatat taaaatggga
     1561 tatggcattg aaaggaaagg tcttcgtatc tatgttgagg atacgggaat cggtataccg
     1621 gatgatttgc atgacaaagt atttacccgt tttgagaaat tgaatgagtt ctcgcagggt
     1681 accggacttg gcttgacgat ctccagagct atcgtagaag cggcaggggg agaagtgggc
     1741 tttacttcaa aacttggtgt cggatctact ttttgggcat ggttgcctct tcgcccttag
     1801 gtctgactcc ccatttattg gtaatgtcct tttaaaatag tgaaggatga gaagcttttt
     1861 tatcgcaatt tatagggatt ttagaactgg tgtagcatat gtgacagaaa tatgacgata
     1921 attctcctac ctttgcggcg gtaaaaatgt ggtattagat aagattgttt atgaaatttg
     1981 agaatgagtg ctgggatcac tttaaaggtg agacctggaa aagagagatc aatgtaagag
     2041 attttatcca gagtaactat acgccttatg aaggggatga ttccttttta gtcgcttctt
     2101 ccgagaagac ccgtagggtc tggaacaagc tgaccgagat gtttaaggtc gagagggaaa
     2161 agggagttta tgacgccgag acaaaattac ctcaagggat cgatgtatat ggcccgggct
     2221 atattgataa ggagaatgag gtgatcgttg ggttacaaac cgatgctccg ctgaaacgtg
     2281 gtatcttccc gaagggcggt atccgtatgg ttgagaatag cttggaagct tatggttatc
     2341 atttggaccc gatgacgaaa gagatcttca cgaagtaccg taaaactcat aacgaaggcg
     2401 ttttctccgc atatacggaa gagatgattg ccgcacgtcg ttcggctatt atcaccggtc
     2461 tgcccgacgc gtatggtaga ggacgtatta ttggtgacta tcgtcgtgtc gctttatatg
     2521 gtacggcaat cttgatcgag gagaaaaagc gtttcttgaa tcgtttggat atccaagaga
     2581 tcacggaaga gatcatccag agccgtgagg agatttccga acagatcaag gctttgaaag
     2641 cgttcgagaa aatgtgcgct agctatggct ttgatgtgac tcgtccggca caaaacgctc
     2701 gtgaggcagt tcagtttgtg tacttagctt atctggcggc ggtaaaagac caagatggcg
     2761 cggcgatgtc tatcggacgt acttctacgt ttttggatat ttatatcgag aaagacatcc
     2821 gtgagggtaa gttgaccgaa gaggaggcgc aagaattggt agaccagctg atcatcaagt
     2881 tgcgtatcgt tcgtttctta cgtacgccgg aatacaatga tttgttctcc ggtgatccgg
     2941 tttgggtaac agagtccttg ggcggccaag gagtggatgg ccgttctttg gtaacaagaa
     3001 cttcctatcg ttatctccat acattatata atctaggtcc ggcaccagaa cccaatttga
     3061 cggttctgtg gtttaagaac gctcccgaga actggaaacg tttctgtgcc aaggtctcta
     3121 tcgatacatc ggctatccaa tatgagaacg acgatttgat gcgtccggat tatggagatg
     3181 attatggtat cgcttgttgc gtttctccta tgaagatcgg taagcagatg cagttcttcg
     3241 gagcccgtgc gaatttggcg aaatgtttat tatatgcgat caacggagga cgggatgagc
     3301 gcagcggcgt acaggtagcc ccgatgttcg agccggttcg cggcgagtat ctggaatacg
     3361 atgaggtgat ggctaagtat gagcaaatga tgcgctggtt ggcgaaagtg tatgtgaacg
     3421 ccttgaagat aatccattat atgcatgata aatacgcata tgaggcattc gagatgtcgc
     3481 ttcacgatgg agatgtagag cgtatccgtg cgacaggtat cgccggtctt tctatcgttg
     3541 ccgattcgtt ggctgctatc cgtgatacga aagtacgtgt gatccgtgac gagcgtggct
     3601 tggcggttga tttcgaacgt gagggcgaat atgttccttt cggtaataac gacgatcgta
     3661 cggatagtat cgctgtagag atcaccgaga agttcatgga atatttgcgt cagcacgaga
     3721 cctatcgcca tgcgaaacct acgcagtcca tccttacgat cacctctaat gtggtatatg
     3781 gtaagaagac tggtacgaca ccggatggcc gtatcggtgg aactcctttc gctccgggcg
     3841 cgaaccctat gaacggtcgt gatacgaaag gtgctatcgc tgcgctagct tcggtagcga
     3901 agcttccatt ccagcatgcg cacgacggta tctcttatac tttcgctgtt tctccggcta
     3961 cgttgggaaa ggagcgtgaa gtgcagatca ataatctggt tagcttattg gatggctatt
     4021 ttactccgga tggaggtcag catctgaatg ttaacgtatt tgataaggat ttgttgatcg
     4081 acgctatgga acatcctgag aaatatccgc aacttacgat ccgtgtatcc ggatatgcgg
     4141 taaacttcgt gaaactgact cgtgaacaac agttggatgt tatttcaaga accattaacc
     4201 attcactgta agtgataaaa ggtaagatac attcattaga gagtttcggg accgtagatg
     4261 gtcccggaat tcgctttgtc gtttttatgc agggctgccc gttgcgttgc ctgtattgcc
     4321 ataacccgga tacttggaac ccgaaaggga aggtgaaata tcagatgact cccggcgagc
     4381 tattgacgga agtcttacgg tataagagtt ttatcgcccg tgggggggtg actgttaccg
     4441 gtggggaacc tttattgcaa cctgagtttc tgaaagagtt tttccgtttg tgccaagagc
     4501 aaggtcttca cacagctttg gacacatctg gtttcgtatg tacctcaaaa gcttgggaag
     4561 tcttggatta tgcggactta gtgttattgg acatcaaaac cttaaacccg gatttacatc
     4621 cgttactggc gggtgtcaag caggataata cgcttctgtt cttggatgaa ttggagagac
     4681 gtggcataga tacttggatt cgccatgtta ttgtccccgg atatacggat aatgacgagt
     4741 ggctggaggc gttagctcgg tatgtcagtt catataaagt ggttcgcaaa gtggaattat
     4801 tgccttatca tacgatggga acctataaat atgagcaatt aggtctcgat tatccgttaa
     4861 agggggtgga acccttatca aaagaacgtt tagataatgc gaaagcgata ttctctcgct
     4921 acaaaccaga aaataacaag gcctgatctc tcgatcaagc cttgcgatta tgcaacagct
     4981 aatcagggaa aacaatctaa tccgattagc ttttaaacga tagtaataat tttttgccta
     5041 gactaacctt gactatttaa tatgtgtttg ttttgttata tgcaacaaat atctattttt
     5101 gtaaggagat aaaaaatgat atagatcaga aaatgatgaa tagtatgaag acaccacaag
     5161 aaatagccga aatgatatcc aaacgttttt atccattacc cgatgcg
//
