LOCUS       c00266_gnlPro..         1559 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|ECPNDDLF_266.
ACCESSION   c00266_gnlPro..
VERSION     c00266_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 07:03:29
            Original ID  :: gnl|Prokka|ECPNDDLF_266
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 1559
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..753)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 53.8;
                     E-value: 8.4e-17)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg266_1"
                     /sec_met_domain="Radical_SAM (E-value: 1.2e-32, bitscore:
                     100.7, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQVFSQNHDPDGESSVTGLVHSVESCGTVDGPGIRFVLFLSGCS
                     LRCRYCHNPDTSYVRRGRLRSADDVLKEIARYRDFLQAAGGGVTLSGGDPLFQPDFAGA
                     VLKGCRKLGLHTCLDTSGHLGVNAGEEMLADTDLVLLDIKAWNPKRYRALTGGELRPTL
                     EFAERLASLRKPVWLRYVLVPGVTDNLEEIEELSRHAALLGNVERVDVLPFHQLGRFKW
                     DELKLDYTLKDVPEASAELAEQVRGIFRR"
     protocluster    1..1559
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:1558]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..1558
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..1559
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..1559
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(770..1558)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg266_2"
                     /sec_met_domain="Gly_radical (E-value: 1.3e-47, bitscore:
                     148.0, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 8.6e-54, bitscore:
                     170.0, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="DALNIIHYMHDKYCYERIEMALHDPEILRTMATGIAGLSVAADSL
                     SAIKYATVKAIRNEEGLIVDFKTEGEFPCYGNNDPRVDDIACSLVSNFMEKLRRLHTYR
                     NSLPTQSILTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDRNGAVASMLSVAKL
                     SYDDSLDGISYTFSIVPQALGKEERERRVKLVSLLDAYFAATGHHINVNVLERETLLDA
                     MDHPEKYPQLTIRVSGYAVNFIKLTREQQQEVINRTFHTR"
ORIGIN
        1 ccgccggaaa atgccgcgga cctgttccgc cagttctgcg gacgcttccg ggacgtcctt
       61 caaagtatag tccagcttca attcatccca tttgaatctc cccagctggt ggaaagggag
      121 cacgtccacg cgttccacgt tgcccagaag cgccgcatgg cgggagagtt cctcaatttc
      181 ttccagattg tccgtaacgc caggtaccag cacatacctc agccacacgg gcttgcggag
      241 ggaggccagg cgttccgcaa actccagtgt agggcgcagt tccccgcctg tcagggcgcg
      301 ataccgtttc ggattccatg ccttgatatc cagcaggacc aggtccgtat ccgccagcat
      361 ttcttcgccc gcattcacgc ccagatggcc ggaggtgtcc aggcaggtgt gcaggcccag
      421 tttccggcag cctttcagaa cggcaccggc aaaatccggc tggaagaggg gatccccgcc
      481 ggagagggtg acgccgccgc ccgccgcctg caaaaaatcc cggtaacggg ctatttcctt
      541 gaggacatca tccgcgctcc tgagccggcc cctccgcacg taggaggtat ccggattatg
      601 gcagtagcgg cagcgcaggc tgcagccgga caggaaaagg acgaagcgga tgccggggcc
      661 gtccacggtg ccgcaggatt ccacggaatg aaccaaaccg gtgacggagc tttctccgtc
      721 cggatcatgg ttttgtgaaa aaacctgcgg catccgcttg cagtccgggt taacgggtgt
      781 ggaaagtgcg gttgatgacc tcctgctgct gttcccgggt cagcttgatg aaattcacgg
      841 catagccgga aacgcggatg gtaagctgcg ggtatttttc cgggtgatcc atggcgtcga
      901 gaagcgtttc ccgttccagt acgttcacat taatgtggtg acctgtagcg gcaaagtagg
      961 cgtccagcag ggagacgagc ttgacgcggc gttcacgttc ctccttgccc agggcctgag
     1021 gaacgatgga gaaggtgtag gagatgccgt ccagggagtc gtcataggac agcttggcta
     1081 cggagagcat ggaggccacg gctccgttcc tgtcccttcc gtgcatgggg ttggctccgg
     1141 gggcgaacgg ttccccggct cggcggccgt ccggcgtgtt gcccgtcttt ttgccgtaca
     1201 ccacgttgga ggtgatcgtc aggatggact gggtgggcag ggaattgcgg taagtgtgga
     1261 gcctgcgcag tttttccatg aaattgctga ccaggctgca tgctatgtcg tccacgcgcg
     1321 ggtcattgtt cccgtaacag gggaattccc cttccgtctt gaagtccaca atcagccctt
     1381 cttcattgcg gatggctttt acggtggcat acttgatggc ggacagggag tccgccgcca
     1441 cggaaagccc ggcgattccc gtagccatcg tgcgcagaat ttccggatca tgcagcgcca
     1501 tttcaatgcg ttcatagcag tatttgtcgt gcatgtagtg gatgatgttc agcgcgtca
//
