LOCUS       c00071_gnlPro..         5152 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KAGBFOJM_71.
ACCESSION   c00071_gnlPro..
VERSION     c00071_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 10:29:49
            Original ID  :: gnl|Prokka|KAGBFOJM_71
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5152
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5152
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:3473]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..3473
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..5152
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5152
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             3..695
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: SucD_FumR"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg71_1"
                     /sec_met_domain="SucD_FumR (E-value: 5.1e-75, bitscore:
                     239.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MWLLNSSIGKKLIMSISGLFLVLFLLFHLSMNVAAVFSGEAYNMV
                     CSLLGSNWYAVAATLVLAAGVVIHFVYAIILTLQNRKARGNDRYAINARPKGVEWASQN
                     MFVLGLIVILFMLLHFSQFWYKMMFAELIGHHEVALGSAMVSPQDGAAFINYYFQGNAV
                     ITVLYLIWYVALWFHLTHGFWSAIQTIGWNNTIWMNRWECISKIVATVICGLFAIITII
                     FFLNGVGA"
     CDS             744..2687
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 194.5; E-value:
                     1.1e-59)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg71_2"
                     /sec_met_domain="FAD_binding_2 (E-value: 9e-61, bitscore:
                     194.5, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 4.2e-39,
                     bitscore: 122.0, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEINSKIPQGPLAEKWTNYKNHQKLVNPANKRRLDVIVVGTGLA
                     GASAAASLAEMGFNVLNFCIQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTIKG
                     GDYRAREANVYRLAEVSNSIIDQCVAQGVPFAREYGGLLDNRSFGGAQVSRTFYARGQT
                     GQQLLLGAYSALSRQIGLGKVKMYTRHEMLDVVKVDGRARGIIARNLITGKIERYAAHA
                     VVVATGGYVNTFFLSTNAMASNGSAAWQCYKKGAYFANPCMVQIHPTCVPVKGDFQSKL
                     TLMSESLRNDGRIWVPKKLEDAKALQAGTKKGKDIPEADRDYYLERRYPAFGNLVPRDV
                     ASRAAKERCDAGFGVNSTGLAVFLDFSDAINRLGKEVVKQKYGNLFDMYEEITNDNPYE
                     TPMMIYPALHYSMGGLWVDYELMTSIPGLFAIGEANFSDHGANRLGASALMQGLADGYF
                     VLPYTIQNYLSDQIQVPRFSTDLPEFVEAEKAIKDRIQKLMNVKGKETVDTIHRKLGHI
                     MWEHIGMARDAKGLQEAIQMLKDLKKEFWSNVFIPGNADNLNTELEKALRVADFIEIGT
                     LMAHDALDRAESCGGHFRTEHQTEEGEALRHDDKFMYVSCWEYQGEDKDPVMLKEDLNY
                     EFVVPQTRNYKK"
     CDS             2718..3473
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg71_3"
                     /sec_met_domain="Fer2_3 (E-value: 5.6e-27, bitscore: 82.5,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 1.1e-12, bitscore: 36.8,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 1.2e-06, bitscore: 17.5,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKDISVTLKVWRQRGPKEKGQFETYKIEKINQSVSFLEMLDILN
                     EQLVNEGKEPIVFDHDCREGICGMCSLYVNGHPHGPATGATTCQLYMRRFHDGETITIE
                     PWRSAGFPVIRDLMVDRYAYDKIIQAGGFTSVNTGGVPDANAIPIPKKDADLAMDAAAC
                     IGCGACAAACKNGSAMLFVSAKVSQLALLPQGKVEAARRAKAMVAKMDELGFGNCTNTR
                     ACEMECPKNISVSNIARLNREFICAKLQD"
     CDS             complement(3510..3620)
                     /locus_tag="ctg71_4"
                     /transl_table=11
                     /translation="MKKPRCKYTDFSIPVKGYNAKLCFTTYNKNKKAPFF"
     CDS             3896..5107
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Radical_SAM"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg71_5"
                     /sec_met_domain="Radical_SAM (E-value: 2.2e-16, bitscore:
                     49.1, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKKRTTSSFRAMSTERKLESIFLFVTGRCNAKCAMCFYAQEMDK
                     KQPDLTFDEIKKISETAGQFNRLWISGGEPTLREDLPEILELFYKNNHIKDVNIPTNGL
                     KPARIVEWVKRFRQNCPDCNINVSLSLDGFGTTHDTQRGVPGNFYKALETLRKIQENFG
                     DDGKVLKNIATVITKYNIDQVEDFMTWVYGRFRTSTHTIEAARGMTRDDGVKILTESTL
                     RKIQDDISPIYSAYADRMVADTTGLRKPITRFFYLGLIRTLYNVRASNIDHPTPWGMDC
                     TAGETTLVIDYDGRFRACELRKPLGNIKEYGCDISKVMNSEAMKQEIAAIGHGYKANCW
                     CTHGCWITSSVIFNPRKMIRSVYKGYRETKRLNHPLAINEQKLQTMEAKYHLDIERLRQ
                     LNIR"
ORIGIN
        1 ttatgtggtt acttaattct tctatcggga agaagctgat aatgagtata tcaggcctct
       61 ttcttgttct gttcttattg ttccacctgt cgatgaacgt ggcggccgtt ttctccggag
      121 aggcttataa catggtttgt tcgctgttgg gctctaattg gtacgctgtg gcagctactc
      181 tcgtattggc cgcaggcgtt gtgatccact tcgtgtacgc tatcattctt accttgcaaa
      241 accgtaaggc acgtggtaac gaccgttacg ctatcaacgc tcgtccgaaa ggcgtggaat
      301 gggcatctca gaacatgttc gttttgggtt tgatcgttat cctgttcatg ctgttgcact
      361 tcagccagtt ctggtataag atgatgttcg ccgaattgat cggtcatcac gaagtagcat
      421 tggggtcagc tatggtatct ccacaagacg gagccgcttt tatcaactat tacttccaag
      481 gtaatgccgt gatcaccgtt ctttatttga tctggtacgt ggctttgtgg ttccacttga
      541 ctcacgggtt ctggagcgct atccaaacaa ttggttggaa taatacgatc tggatgaacc
      601 gttgggaatg tatctctaag atcgtagcta cggttatttg cggacttttc gcgatcatta
      661 ctatcatctt cttcctaaac ggagtaggtg cttaattgca agctacaggt tttataatta
      721 agaacttttg aaattaaatc attatgtctg aaataaattc taaaatacct caaggcccgt
      781 tggctgagaa gtggactaat tataaaaatc accagaaatt ggtgaacccc gctaacaagc
      841 gtcgtttgga tgttatcgta gtcggtacag gtttggccgg agcttccgct gccgcatctt
      901 tggcagagat gggattcaac gtattgaact tctgtatcca agattctccc cgccgtgcgc
      961 actctatcgc cgctcaaggt ggtatcaacg ccgctaagaa ctaccagaat gatggtgact
     1021 ctgtttaccg tttgttctac gatacgatca aaggtggtga ctaccgtgct cgtgaggcta
     1081 acgtttatcg tttggctgag gtttctaact caatcatcga ccagtgcgtg gctcaaggcg
     1141 ttcctttcgc tcgcgaatat ggtggtttgt tggataaccg ttcattcggt ggcgctcagg
     1201 tatcgcgtac gttctacgcc cgtggccaga cgggtcagca gcttttgtta ggcgcttatt
     1261 cagcgttgag ccgtcagatc ggtttgggta aggttaagat gtacactcgt catgagatgt
     1321 tggatgtagt taaggtagac ggtcgtgctc gtggtatcat cgcccgcaac ttgattacag
     1381 gtaagatcga gcgttacgcc gctcacgctg tagttgtagc tacgggtggt tacgttaata
     1441 cgttcttctt gtcaaccaac gctatggcat ctaacggttc cgccgcatgg caatgctaca
     1501 agaaaggcgc ttacttcgct aatccttgta tggttcagat ccacccgact tgcgtaccgg
     1561 tgaaaggtga cttccaatct aaattgactt tgatgtccga atccttgcgt aacgacggcc
     1621 gtatctgggt tccgaagaaa ctggaagacg ctaaagctct acaggccgga acgaagaaag
     1681 gtaaagatat tcccgaggca gaccgtgatt attatttgga gcgtcgttat ccggcattcg
     1741 gaaacttggt tcctcgcgac gtggcttctc gtgccgctaa agagcgttgc gacgcaggtt
     1801 tcggcgtgaa cagcacgggc ttggccgtat tcttggattt ctccgacgcg atcaatcgct
     1861 tgggtaaaga ggtcgttaag cagaaatacg gtaacttgtt cgatatgtac gaagagatca
     1921 cgaacgataa tccatacgag actccgatga tgatctaccc ggcattgcac tactcaatgg
     1981 gtggattgtg ggttgactat gagttgatga cctccattcc gggtctgttc gctatcggtg
     2041 aggcaaactt ctccgatcac ggagcgaacc gtttaggtgc ttccgctttg atgcagggat
     2101 tggccgatgg ttatttcgta ttaccataca cgatccagaa ctatttgtcc gaccaaatcc
     2161 aagttcctcg cttcagcacg gatctgccgg agttcgtcga ggcagaaaag gccatcaagg
     2221 atcgtatcca aaaattgatg aacgtcaagg gtaaagaaac cgtagacact atccaccgca
     2281 aactaggtca tatcatgtgg gaacatatcg gtatggcacg cgacgcaaaa ggtttgcaag
     2341 aagctatcca gatgctgaaa gacttgaaga aagagttctg gagcaacgta ttcatcccag
     2401 gcaacgcaga caacttgaat accgagttag agaaagcatt acgtgtagcc gactttatcg
     2461 agatcggtac attgatggca cacgatgctt tggaccgcgc tgagtcttgt ggtggtcact
     2521 tccgtacgga gcaccagaca gaagaaggtg aggccttgcg tcatgacgat aagttcatgt
     2581 acgtatcttg ctgggagtac caaggcgagg acaaagatcc tgtcatgttg aaagaggatt
     2641 tgaactatga gttcgttgtt cctcaaacac gtaactacaa gaagtaataa tcatcaatct
     2701 cagaaaaaat tagaatcatg gataaagata taagtgtaac acttaaggtt tggcgtcaga
     2761 gaggtccgaa ggagaaagga cagttcgaga catacaaaat tgagaagatc aatcagagtg
     2821 tctctttctt ggaaatgttg gatatcttga acgagcagtt ggtcaacgag ggtaaggaac
     2881 cgatcgtatt cgatcacgac tgccgcgagg gtatctgtgg catgtgttca ttgtatgtaa
     2941 acggacaccc gcacggaccg gcaactggag ctacgacttg ccagttatat atgcgtcgtt
     3001 tccacgatgg agagacgatc acgatcgaac cgtggcgttc cgcaggtttc cctgttatcc
     3061 gcgacttgat ggtagaccgc tacgcttacg acaaaatcat ccaagcgggt ggtttcacat
     3121 ctgtcaacac aggtggtgta cccgacgcta atgcgatacc tatcccaaag aaggacgcag
     3181 acttagccat ggacgctgcc gcttgtatcg gttgtggcgc ttgtgctgcc gcttgtaaga
     3241 atggttccgc tatgttattc gtatccgcta aggttagcca gctggctttg ttgcctcaag
     3301 gtaaagtcga ggctgctcgc cgtgccaaag ctatggtcgc taaaatggat gagcttggct
     3361 tcggtaactg tacgaacaca cgtgcttgcg agatggaatg tccgaagaat atctccgtta
     3421 gcaacatcgc tcgtttgaac cgcgagttca tttgcgctaa attacaagac taatatttga
     3481 ttagtatatc atggaaaagg cgtctttctc taaaagaaag gcgccttttt gtttttgtta
     3541 tatgtcgtaa aacataattt agcattatag cccttgacag gaattgaaaa gtccgtatat
     3601 ttgcatcgtg gttttttcat aatagtatta gatttaaggt taacaaagtt tggttagggc
     3661 ttgtcgtgag acaggcttta attgtttata gacctacctt ttaaacatgg accataaaat
     3721 actgcaaata gagattgttt agcgtcattt tatcgtcgat ctttgccttt aaagatcata
     3781 tagtatatgc taataagtat tttatttggc ttcttatgtt attttctccg ggtgttgatc
     3841 aaacacaagg aaaaaatcat gcgccagata agacaaatgg taaaacaaaa ataaaatgga
     3901 caaaaaacga accaccagtt ctttcagggc aatgagcacg gaaagaaaac tcgagtcgat
     3961 cttccttttc gtaacaggcc gatgcaacgc caaatgcgcg atgtgtttct atgcccaaga
     4021 aatggataag aagcaacccg acctcacttt cgatgaaatc aagaagatat cagaaaccgc
     4081 cgggcaattc aaccgattat ggatatccgg aggtgagcct accctgcgtg aggatctacc
     4141 ggaaatattg gagttattct acaaaaacaa ccatatcaaa gatgtgaaca tccctacgaa
     4201 cggcttaaaa ccagcccgga tcgtcgaatg ggtaaaacgg ttccgccaga actgcccgga
     4261 ttgtaatatt aacgtaagcc tttccttgga tggtttcggt acgacccacg atacacagag
     4321 aggcgtaccg ggaaacttct ataaggcctt ggaaaccctg cgtaagatac aggagaattt
     4381 cggagacgac ggcaaggtcc tgaaaaatat agcaaccgtt atcacgaaat acaatataga
     4441 ccaggtagaa gacttcatga cttgggtata cggacgtttc cgcacctcca cgcatacgat
     4501 tgaggccgcc agaggcatga cccgtgacga tggcgtgaaa atcctaaccg agtcaacgct
     4561 acgcaaaata caagacgaca tatcccctat ctattctgct tatgcggatc ggatggtagc
     4621 ggataccacc ggcttaagga aaccgatcac acgcttcttc tatctcggcc ttatacgtac
     4681 cttatataat gtgcgtgcca gcaacataga tcacccgacc ccttggggga tggattgtac
     4741 ggccggagag actaccttgg taatcgacta cgacggacgt tttcgtgcct gcgagctacg
     4801 gaaaccgctc ggaaatataa aagagtatgg ttgcgatata agtaaagtga tgaatagcga
     4861 agcgatgaaa caggaaatag ccgctatcgg acatgggtat aaagccaact gttggtgtac
     4921 gcacggctgc tggatcacct catccgtcat tttcaatcca cgtaagatga ttcgctccgt
     4981 atataaagga taccgggaaa cgaaacgatt gaatcatccc ctagccatca atgagcagaa
     5041 actacaaacg atggaagcca aataccatct ggacatcgag cgtttgcgtc aactgaatat
     5101 ccggtgaaca agaccctcat agcccataaa taaacgtagc atgaaaatac ta
//
