LOCUS       c00140_gnlPro..         6896 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NENKFPGP_140.
ACCESSION   c00140_gnlPro..
VERSION     c00140_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 07:44:58
            Original ID  :: gnl|Prokka|NENKFPGP_140
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6896
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6896
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3517:6895]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3518..6895
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..6896
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6896
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             complement(157..741)
                     /locus_tag="ctg140_1"
                     /transl_table=11
                     /translation="MTTKLKDLFDTFLNIAASSQKLGINLIRVAILIIFVWIGGLKFYN
                     YEAEGIVPFVANSPFMSFFYAKSAPEYKEYKLKEGEFNEAKHQWHVENNTYIFSHGLGI
                     MIMAIGILTFLGIFSPKIGLIGSGLAIIMTLGTLSFLVTTPEVWVPDLGSGEHGFPLLT
                     GAGRLVIKDTAIIAGAIVVLSDCAQRILKKK"
     CDS             complement(919..2133)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Radical_SAM"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg140_2"
                     /sec_met_domain="Radical_SAM (E-value: 8.4e-15, bitscore:
                     44.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTKRTKSSFRTMSTNRKLESVFLFVTGRCNAKCKMCFYAEDMDK
                     KEKDLTFEEIKTISETAGQFNRLWVSGGEPTLREELPEIIEMFYKNNEIKDVNIPTNGL
                     KPDRIVAWVKQIRQNCPELNINLSLSLDGFGDTHDIQRGVPGNFYKAMGTLKLIEKNFA
                     DDGRVLKNLATVITKYNENEVEDLMIWMLGRFHLSNHIIEAARGMTRDDGVKVLTEPTL
                     RALQDKVVPIYNVYGERMAEETGGFRKPIARFFYLGFIRTLYNVRATNIDHPTPWGMDC
                     TAGETTLVIDYDGRFRACELREPLGNVKDYDCNIAKIMESDAMKQEIAAIGHGDKANCW
                     CTHGCWITSSLVFNPRKMITQVYKGYREMKKLNRPLNISEELLQSIERKYALDEQKLVE
                     LGVRN"
     CDS             2374..3219
                     /locus_tag="ctg140_3"
                     /transl_table=11
                     /translation="MEDNIPKFDLPVDFIVGNSITGEILNQYGRFPCKIKAGIFVLCVQ
                     GTVHATVNLTEFTIRKNDFITLPPGCFIQIHEVSDDVKLCFAGFSSVFVSHINYIKTIT
                     NYLPVIFDSPIMPLPEHISLLYQQAFDLLINAYSMPKTIENKEIIKAIFTIFMQGVIEL
                     YRNHTPYSNAPMTRNMEICREFVQLAMENYTKEHSVSYYAKRLNITLQHFCYVIKKVSG
                     RTALDILSNIIIMDAKAQLKSTDMPVKKIAFALGFDNLSFFNKYFRQHVGMTPQEYRES
                     "
     CDS             complement(3518..4273)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer4_7"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg140_4"
                     /sec_met_domain="Fer4_7 (E-value: 1.7e-12, bitscore: 36.6,
                     seeds: 208, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_3 (E-value: 4.1e-27, bitscore: 83.2,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 1.2e-06, bitscore: 17.7,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKDIKVTLKVWRQKSPKDKGQFETYQIDKINQSVSFLEMLDILN
                     ERLVSEGKEPIVFDHDCREGICGMCSLYVNGHPHGPATGATTCQLYMRRFNDGETITIE
                     PWRSAGFPVIRDLMVDRYAYDKIIQAGGFVSVNTGGVPDANSIAIPREVADVAMDSATC
                     IGCGACAAACKNGSAMLFVSAKVSQLALLPQGKVEAARRAKAMVAKMDELGFGNCTNTR
                     ACEMECPKNISVSNIARLNREFICAKLQD"
     CDS             complement(4301..6244)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 195.7; E-value:
                     4.7e-60)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg140_5"
                     /sec_met_domain="FAD_binding_2 (E-value: 4.5e-61, bitscore:
                     195.7, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 4.5e-39,
                     bitscore: 122.1, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQIDSKIPQGPLAEKWKNYKDHQKLVNPANKRRLDVIVVGTGLA
                     GASAAASLAEMGFNVLNFCIQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTIKG
                     GDYRAREANVYRLAEVSNSIIDQCVAQGVPFAREYGGLLDNRSFGGAQVSRTFYARGQT
                     GQQLLLGAYSALSRMIGLNKVKMYTRHEMLDVVKVDGRARGIIARNLITGQIERFSAHA
                     VVVATGGYVNTFFLSTNAMASNGSAAWQCYKKGAYFANPCMVQIHPTCVPVKGDYQSKL
                     TLMSESLRNDGRIWVPKKLEDAKALQAGTKKGKDIPEADRDYYLERRYPAFGNLVPRDV
                     ASRAAKERCDAGFGVNNTGLAVFLDFSDAINRLGKDVVKAKYGNLFDMYEEITNDDPYE
                     TPMMIYPALHYSMGGLWVDYELMTSIPGLFAIGEANFSDHGANRLGASALMQGLADGYF
                     VLPYTIQNYLSDQIQVPRFSTDLPEFAEAEKGIKDRIKKLMSIKGKESVDSIHKKLGHI
                     MWEHVGMARDAKGLQEAIQMLKELKKEFWSNVYIPGEADNLNVELDKALRLADFIEIGT
                     LMAHDALDRAESCGGHFRTEHQTEEGEALRHDDKFMYVSCWEYQGEDKDPVMLKEDLNY
                     QFVVPQTRNYKK"
     CDS             complement(6278..6895)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: SucD_FumR"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg140_6"
                     /sec_met_domain="SucD_FumR (E-value: 5.8e-77, bitscore:
                     246.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="FSGEAYNMICSLLGANWYAVAATLVLAAGVVIHFVYAVLLTLQNR
                     KARGNDRYAINARPKGVEWASQNMLVLGIIIILFMVLHFSQFWYKMMFAELLGNHMVTL
                     CDGTTTVSPQDGAAFINYYFQGNILNTVLYLVWYVALWFHLTHGFWSAIQTIGWSNTIW
                     MNRWICISKIVATVICVVFAIITIVFYLNGVGGDYVAVCAGH"
ORIGIN
        1 ctattatgaa aaaaccacga tgtaaatata ctgcttaatc caaacctgtc aagaggtttt
       61 atgctaaatg gtgtttcata cactataaga aaagctaaca aggttcaata tgtttgtttt
      121 ttgcttccaa agtgttaaaa taaacacttt ttgttcctat ttcttcttta atatacgttg
      181 tgcacagtcg gacaatacca cgatcgctcc ggctatgatt gccgtatctt ttattaccag
      241 ccgacctgct ccggtcagta aggggaaacc atgttctccg cttcccaaat cgggcaccca
      301 tacttcaggt gttgtcacca ggaatgaaag cgtacccaat gtcattatga tcgccagtcc
      361 ggaacctatc aagccaattt tcggagaaaa gatacccagg aaagtcagga taccgatcgc
      421 catgatcata atgcctagtc catgtgaaaa aatataggta ttgttttcta catgccactg
      481 atgcttggct tcattgaatt caccttcttt caacttatat tctttgtatt ccggtgcact
      541 ctttgcatag aaaaagctca taaacggact gtttgccaca aaaggaacga tcccttccgc
      601 ttcataatta tagaatttca gcccgccgat ccatacgaat ataatcagga tagctacgcg
      661 gatcaggtta atacccagtt tctgtgaaga agctgcaatg ttcagaaatg tgtcgaataa
      721 atcttttaat ttagtagtca tgtctttctt tgtttatcag tttaacttat gatgcaaaga
      781 tccgacattt ttcccctatg ggaaatggca attctaccag ataatttgac aattattccc
      841 ttaaacacaa taaagccggt taacagaggt atatttcccg tcaaccggct ttataaatga
      901 tccgttatcg gaactatttt aattacgaac ccccagctcc accagtttct gttcgtccaa
      961 ggcatatttg cgttcgatgc tctgcaaaag ctcttcactg atattcagag gacggttcag
     1021 cttcttcatc tcccggtatc ctttatagac ctgcgtgatc atcttgcggg gattgaaaac
     1081 aagcgatgaa gtgatccaac acccatgcgt acaccagcag ttagccttgt caccatgacc
     1141 gatagcggca atctcctgtt tcatggcatc gctctccata atctttgcga tattgcaatc
     1201 ataatccttt acattaccga gcggttcgcg aagttcgcag gcacggaaac gaccgtcata
     1261 gtctattacc agtgttgttt cgccggccgt acaatccata ccccaggggg tcggatgatc
     1321 gatattggtc gcacgtacat tatataatgt acgtatgaaa cccaggtaga agaaacgggc
     1381 gataggtttt ctgaacccac cggtttcctc cgccatacgc tctccatata cattataaat
     1441 aggaacaacc ttatcctgca aggcgcgtaa agtaggctcg gtaagaacct tcactccgtc
     1501 gtcgcgggtc atgccgcgtg cagcttcgat aatatgattg gaaagatgga aacgtcccag
     1561 catccatatc atcagatcct cgacctcatt ctcgttatat ttggtgatta cggtggccag
     1621 gtttttcaac accctgccgt cgtcagcaaa gtttttttca atcagcttca gtgtccccat
     1681 cgctttgtag aaattgccgg gaacgccgcg ctgtatatca tgcgtatcac cgaaaccgtc
     1741 caaagaaagg ctcaggttga tattcagttc cggacaattc tggcggatct gttttaccca
     1801 ggcgacgatc cggtcaggct tcaatccgtt ggtagggata ttcacgtcct ttatctcgtt
     1861 attcttgtag aacatctcta taatttcagg taactcctca cgcagtgtcg gttcaccgcc
     1921 ggatacccac aaacggttga actgcccggc tgtttcggag attgtcttga tttcttcaaa
     1981 agtcaggtct ttctcctttt tatccatgtc ttctgcatag aaacacattt tacatttagc
     2041 attacaccgt cctgtgacaa acaggaaaac agattctaat tttctgtttg tgctcattgt
     2101 cctgaaagag ctctttgttc ttttcgtact cattgttatt atcgattaga tttctctatt
     2161 agtattagct cccatatctt atttagcaga tagacaaata tataccatcc caacaaatct
     2221 atcagcaaga cttgaaaaca ttcgcaaatt tcaactatta tacagcacaa aacaatctcc
     2281 atttgtggga ttcgatgttc tatatttaag attatcgact aacttgtctc aaaaaaacta
     2341 tctttgtaca ttcaattaag tgtatataaa agtatggaag acaatattcc taaattcgac
     2401 ctccccgtag attttattgt aggaaacagt attacaggtg aaatactgaa ccaatacgga
     2461 cgtttcccgt gcaagatcaa ggcaggcata ttcgtgcttt gcgtacaagg gacagtccat
     2521 gcgactgtaa acttaactga gtttaccatc cggaaaaacg actttatcac cttgcccccg
     2581 ggttgcttca ttcagataca tgaggtttcc gatgatgtaa aactgtgttt tgcaggcttt
     2641 tcttccgtat tcgtcagcca tatcaattat ataaagacga ttacgaacta tctgcctgtc
     2701 atattcgaca gtccgatcat gcctttgccg gagcatatat ccttactcta ccaacaggct
     2761 ttcgacttgc tgatcaatgc ttattccatg cctaaaacca tcgaaaacaa ggagattatc
     2821 aaggctatct ttactatctt catgcaaggg gttattgaat tatacaggaa tcatacgcca
     2881 tatagcaatg ctcccatgac gcgtaatatg gaaatatgcc gggagttcgt acagctggcg
     2941 atggaaaact acacgaaaga gcatagcgtg tcttattacg ccaaaaggct caacatcacc
     3001 ttgcagcatt tctgttatgt gataaaaaag gtcagcggac ggactgcatt ggatatcctg
     3061 agcaatatca tcattatgga tgccaaagcg cagcttaaat cgaccgatat gccggtcaag
     3121 aaaattgcat tcgccctggg cttcgacaac ctttcgttct tcaacaaata tttccggcaa
     3181 catgtaggaa tgactccgca ggaatacagg gagagctaaa aaaagggata tgccggacgc
     3241 aaacccctat aaacaattaa agcctgtctc acgacaagcc ctaaccaact ttgttaacct
     3301 taaatctaat actattatga aaaaaccata gtacaaagat acaggtattc tccaacttgt
     3361 caatagttaa aatgctaatt agtgttgtat agcatataag aaacactaac aacattcaag
     3421 atatttatct atgacttcag aaatgttaaa ataggggcaa aaaagaagag gcgccgccat
     3481 aagcaacgcc tctccggaca tacatatata atgtatatta gtcttgcaat ttagcacaaa
     3541 tgaactcgcg gttcaaacga gcgatgttgc tgacagagat gttcttcgga cattccattt
     3601 cgcaagcacg cgtgttggta cagttaccga agcccagttc gtccatcttg gcaaccattg
     3661 ctttcgcacg acgagcagct tctaccttac cctgaggcaa cagagccaac tggctaacct
     3721 ttgcagaaac gaacaacata gccgaaccgt tcttacaagc agcagcacaa gcaccgcaac
     3781 cgatacatgt agcggagtcc atggcaacgt cggctacttc acgaggaata gcgattgaat
     3841 tggcatcagg tacaccacct gtgttcactg atacgaaacc acctgcctgg ataattttat
     3901 cgtaagcata acggtctacc atcaagtcgc ggattaccgg gaaaccggca gaacgccacg
     3961 gttcgattgt aatagtttcg ccatcattga agcgacgcat ataaagctgg caagtagttg
     4021 cgccggttgc aggtccgtgc ggatgtccgt ttacatacaa agaacacata ccgcagatac
     4081 cttcacgaca gtcgtgatcg aatacgatcg gctctttacc ttcactgacc aaacgttcat
     4141 tcaaaatgtc taacatttcc aggaaagaaa cactctgatt gattttgtct atttggtaag
     4201 tctcaaattg tcctttatct ttcggacttt tctgacgcca aactttaagt gttactttta
     4261 tatctttatc catgattcta atttttgtga ttgatgatta ttacttcttg tagttacgtg
     4321 tttgaggaac aacgaactga tagttcaaat cttccttcag cataaccgga tctttgtctt
     4381 caccctggta ttcccaacaa gatacgtaca tgaacttatc gtcatgacgc agggcttcgc
     4441 cttcctcggt ctggtgctct gtacggaagt gtcctccaca agattcggca cggtccaatg
     4501 catcgtgtgc catcaatgta ccaatctcaa taaagtcagc caaacgcaat gccttatcca
     4561 actctacgtt caggttatcg gcttcacccg gaatatatac attactccag aactctttct
     4621 tcagctcttt caacatctgg atcgcttcct gcaaaccttt tgcatcacgt gccataccta
     4681 catgttccca catgatatga cccagtttct tatggataga atctaccgac tctttaccct
     4741 tgatactcat caatttcttg atgcggtctt tgatcccctt ttcagcttct gcaaactcag
     4801 gcaggtcggt actgaaacgc ggaacctgga tctggtctga cagatagttc tggattgtat
     4861 aaggcaatac aaagtagcca tcggccaatc cctgcatcaa cgcagaagca cccagacggt
     4921 tagctccgtg gtcagagaag ttagcttcac cgatagcgaa cagacccgga atagaagtca
     4981 tcaattcata gtcaacccac agaccaccca tcgagtagtg caatgcagga taaatcatca
     5041 tcggagtttc gtatggatcg tcgttggtaa tttcttcgta catgtcgaac aagttaccat
     5101 atttagcttt tacaacatct ttacccagac ggttgatggc gtcggagaag tcgaggaaca
     5161 cggccagacc tgtgttgttc acaccgaagc cggcgtcgca acgttcttta gcggcacgtg
     5221 aagccacgtc acgcggaacc aggttaccga atgccggata acggcgttcc agatagtagt
     5281 cgcggtctgc ttcaggaatg tctttccctt tcttagttcc ggcctgtaat gcttttgcat
     5341 cttccagttt cttcggaacc cagatacgac cgtcgttacg caatgattca gacatcaaag
     5401 tcagcttaga ctggtagtca cctttcaccg gtacacaagt cgggtggatc tgcaccatac
     5461 aagggttagc gaagtaagca cctttcttgt agcactgcca tgcagcagaa ccgttggaag
     5521 ccattgcgtt agtagacagg aagaaagtat ttacataacc acctgttgcc accactactg
     5581 cgtgagcaga gaaacgttcg atctgacctg tgatcaggtt acgtgcgatg ataccgcggg
     5641 cacggccatc caccttcaca acgtccagca tttcgtggcg ggtatacatc tttactttgt
     5701 tcaaaccgat catacggctc aaagcagagt aagcacccag caacaactgc tgtccggtct
     5761 gaccgcgggc atagaacgta cgggatacct gagcaccacc gaatgaacgg ttatccagca
     5821 gaccaccgta ttcgcgagcg aaaggaacac cctgtgctac gcactggtcg ataatagagt
     5881 tggaaacttc agccagccga taaacgtttg cttcgcgggc acgatagtca ccacctttga
     5941 ttgtatcgta gaacagacgg taaacagagt caccatcatt ctgataattc tttgcagcat
     6001 tgataccacc ctgtgcggcg attgagtgtg cgcgacgggg agaatcctgg atacagaagt
     6061 tcaatacgtt gaatcccatt tctgccagag aggcagcagc agaagcaccg gccagaccag
     6121 taccaactac aatcacgtcc agacgacgtt tgttcgcggg gttcaccagt ttctgatgat
     6181 ctttataatt tttccacttc tcagccaacg ggccctgagg gattttagaa tctatttgag
     6241 tcataatgat ttattttcaa tattgttaca ccttaaatta atgtcctgca caaacagcta
     6301 catagtcacc acctacacca ttcaggtaga aaacgatagt aatgatagca aacactacgc
     6361 aaataacggt tgctacaatc ttggaaatgc agatccaacg gttcatccag atcgtgttac
     6421 tccaaccgat agtctgaata gcgctccaga acccgtgagt caggtggaac cacaaagcta
     6481 cgtaccatac cagatacagc actgtattca ggatattacc ctggaaatag tagttgataa
     6541 acgctgcacc gtcctgagga gatacggttg ttgttccgtc gcaaagtgtt accatgtgat
     6601 tgcctaggag ttcggcgaac atcatcttat accagaactg gctgaagtgc aacaccatga
     6661 acaggataat gataataccc agtaccagca tgttctgaga tgcccattcc acacctttcg
     6721 gacgagcgtt aattgcataa cggtcgttac cgcgagcctt acggttttgc agtgtaagga
     6781 gaacagcata cacaaagtga atcacaacgc ctgctgccag caccaatgtt gctgccacgg
     6841 cataccaatt tgctcccaac aatgaacaaa tcatgttata ggcttctccc gagaaa
//
