LOCUS       c00198_gnlPro..         5557 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NENKFPGP_198.
ACCESSION   c00198_gnlPro..
VERSION     c00198_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 07:44:58
            Original ID  :: gnl|Prokka|NENKFPGP_198
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5557
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5557
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[840:3817]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      841..3817
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5557
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5557
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             841..3081
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg198_1"
                     /sec_met_domain="Gly_radical (E-value: 1.1e-45, bitscore:
                     142.8, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 3.4e-285, bitscore:
                     935.1, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENENWAQFKGEIWKREINVRDFIQNNYKPYEGDDSFLKDSSD
                     KTKKVWDKLTEMFKVEREKGVYDAETKYPQGIDTYGPGYIDKENEVIVGLQTDAPLKRG
                     IYPKCGTRMVENSLEAYGYKLDPMTKEIFTKYRKTHNEGVFSAYTDEMLAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTEILIAEKKHYLKRLDIQELTEEIIQSREEISEQIKAL
                     KAFEKMCAGYGFDVTKPAKDAREAVQFVYLGYLAAVKDQDGAAMSLGRTSTFLDIYIEK
                     DIREGKLTEEEAQELIDQFIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTKTSYRYLHTLYNLGPAPEPNLTVLWFNNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQYFGARANLAKCLLYAINGGRDERSGVQVAPRFEPITS
                     EYLDYNEVMEKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMALHDGSVERIRATGI
                     AGLSIVADSLAAIRDTKVKVIRDERGLAVGFEREGDYVPFGNNDDRTDSIAVDITEKFM
                     EYLRQHQTYRNATPTQSILTITSNVVYGKKTGATPDGREGGTPFAPGANPMNGRDTKGA
                     VAALASVAKLPFQHAHDGISYTFAVSPATLGKERDIQIANMVGLLDGYFTPDGGQHLNV
                     NVFDKELLLDAMEHPENYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             3089..3817
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.3;
                     E-value: 1.5e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg198_2"
                     /sec_met_domain="Radical_SAM (E-value: 3.6e-37, bitscore:
                     116.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LEIKGNIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWDS
                     KGLAKYQMTPEELLAEVLRYKNFIAKGGVTVTGGEPLLQAEFLREFFRLCREAGIHTAL
                     DTSGYICTPKALAVLEYADLVLLDIKTIDPDLHPRLTAVKLDNTLRFLDELEKRGVPVW
                     IRHVIVPGLTDNDEALDKLAEYISHYTVVQKAELLPYHTMGTYKYEAQGLDYKLKEVEP
                     LSAERLANAKEIFKKHGITI"
     CDS             3906..4808
                     /locus_tag="ctg198_3"
                     /transl_table=11
                     /translation="MEQHHHDHHHHEITSLNKAFIIGISLNLAFVVAEFTAGFWYNSLG
                     LLSDAGHNLGDVASLVLAMLAFRLAKVHPTCSYTYGYKKSTVLVSLLNAVILLVAVGII
                     IAESIGKLLHPRPVEGDAIAWVAGIGVVINALTAWLFMKDKDKDLNVKGAYLHMAADAL
                     VSVGVVVSGIVIAHTGWYIIDPIIGLLIAFVIIVSTWGLLRDSIRLSLDGVPAGTDIDK
                     VEKTIHSVEGVEGVHHLHIWAISTTETALTAHVSVADISEMERIKQEIKHDLQHVGIQH
                     ATLEFETSGFKCDGRCCSD"
     CDS             4969..5535
                     /locus_tag="ctg198_4"
                     /transl_table=11
                     /translation="MEPIINSQAPEFKVQAYHNGSFKTVSSDDIKGKWAIFFFYPADFT
                     FVCPTELVDMADKYDQFQAMGVEVYSVSTDSHFVHKAWHDASETIRKIKYPMLADPTGV
                     LSRAFGVMIEEDGMAYRGTFLVNPEGKIKLAEINDNGIGRNADELLRKVEAAQFIAEHP
                     SEVCPAKWKKGGETLKPSIDLVGKI"
ORIGIN
        1 ggcgggcagg tctgtggtat atgttgcggg ggaaaatctg aaggtagaat aattatatag
       61 gtaatatttt accctgtaat ccctatttat ggttagaaat gagctcgttt tacatataaa
      121 taggtattgc ctgtgccttt ccgtggtttt acctttgccg tatcaattaa gttttactat
      181 tagtataaat cgtcctgacc aaaatccgat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnat ttaaggtaaa tacccatgga agggagtttt tttattggtt gtccctttca
      301 tatataaaag ggagccatcc tgtgaagaat ggctcttttt tatttatcaa agaaagcaat
      361 ttttgcaaat attgtttatt ccaatgatca attttcccga ttataacaca gaataggtag
      421 taggaagctc tttttttact atataatagg tattgtgtac tccccttgac ggctctatct
      481 ttgcaacata gaaaaaagta acactattag tataatcgct atatcaaaac cttttgctaa
      541 ttatgtaagc atataagatg attaaacgtg tgtcggaaat tttttattgg ttgtttccgg
      601 cataaaaaag ggactctcct gcgaaggatg gttccttttt tatttttttg atattattta
      661 taatccttcc tgtttgatac ctatatcagg cgatacgctg cactatatct accgttttag
      721 taactatttg tagggatttt ggccgttggg aatacatgat gtttttgttg attgtttcgc
      781 atgccatata aataacgtac ctttgcaccg aaattaaaac cgaagaaaac aaaataaggt
      841 atgaagtttg aaaacgagaa ctgggcgcag ttcaaaggcg aaatttggaa aagagagatt
      901 aacgtaaggg attttatcca gaacaactac aaaccttacg aaggggacga ctctttcttg
      961 aaagactcct cagataagac gaagaaagtt tgggataaat tgaccgaaat gtttaaggtt
     1021 gaaagggaga agggtgttta cgacgctgaa acaaaatatc ctcaaggtat agatacttac
     1081 ggaccaggtt atattgacaa agaaaacgaa gtaattgtcg gcctgcaaac cgatgccccc
     1141 ctgaaacgcg gtatttaccc gaaatgtggt acccgcatgg tggaaaacag tctggaagca
     1201 tatggctaca aactggaccc gatgacaaaa gaaatcttca cgaaatacag aaagacgcat
     1261 aacgaaggcg tattctccgc ttatacagat gaaatgcttg cagcgcgccg ttccgctatc
     1321 atcaccggcc tgccggatgc ttacggacgc ggacgtatta tcggtgacta ccgccgtgta
     1381 gcactttacg gtactgaaat cctgatcgct gaaaagaaac attatctgaa aagactcgat
     1441 attcaggaac tgaccgaaga gatcatccag agccgtgagg aaatatccga acagatcaag
     1501 gcgctgaagg ctttcgaaaa gatgtgtgcc ggttatggtt tcgacgtgac caagcctgca
     1561 aaggatgcac gcgaagcggt tcagtttgtc tatctgggtt atctggctgc ggtgaaggac
     1621 caggatgggg ctgccatgtc gttgggacgt acttctactt tcctcgatat ttatattgaa
     1681 aaagatatcc gcgaaggcaa gctgacggaa gaagaagctc aggagctgat cgatcagttt
     1741 attatcaaac tgcgtatcgt ccgtttcctt cgtactccgg aatataacga cctgttctcc
     1801 ggtgaccctg tttgggtaac cgagtctttg ggcggacagg gtgtcgacgg ccgttcattg
     1861 gtgacgaaga cgtcttaccg ttatctgcat acattatata acctcggccc ggcaccggaa
     1921 cccaacttaa cggtactgtg gttcaacaac gctcctgaaa actggaaacg tttctgtgcg
     1981 aaggtttcta tcgatacttc cgctatccag tatgagaacg acgatctgat gcgtccggat
     2041 tatggagatg attatggtat tgcctgctgc gtgtctccga tgaagatcgg taagcagatg
     2101 cagtatttcg gcgcacgtgc caacctggct aaatgtttgc tgtatgccat caacggcggg
     2161 cgcgacgaaa gatcaggtgt acaggtggct ccgaggtttg aaccgatcac cagcgaatac
     2221 ctggattaca acgaagtgat ggagaaatac gaacagatga tgcgctggct ggctaaagtt
     2281 tatgtgaatg cactgaagat catccattac atgcacgata aatatgcgta tgaagcattc
     2341 gagatggctt tgcatgacgg ttcggtagaa cgtatccgcg caacaggtat tgccggcctt
     2401 tctatcgttg ccgactcatt ggctgctatc cgcgatacga aagtaaaagt gatccgcgac
     2461 gaacgtggcc tggctgtcgg tttcgaacgt gaaggagatt atgttccttt cggtaacaat
     2521 gacgatcgta cagatagtat tgctgtcgac atcactgaaa agttcatgga atatctgcgt
     2581 cagcaccaga cataccgcaa cgccactccg acacagtcta tcctgactat cacatccaat
     2641 gtggtgtatg gaaagaagac aggtgcaact cccgacggtc gcgaaggtgg tacgccgttt
     2701 gctccgggtg cgaacccgat gaacggacgt gataccaaag gcgctgttgc tgcactggct
     2761 tctgttgcta aactgccgtt ccagcatgca cacgacggta tctcttatac attcgctgtt
     2821 tctcctgcaa cgttaggaaa agaacgtgat attcagatcg ccaatatggt cggcctgctg
     2881 gacggttact ttactcctga cggcggacag catctgaatg tgaacgtatt cgacaaggag
     2941 ctgttgctcg atgctatgga gcatccggaa aactatccgc agttgacaat ccgcgtatcg
     3001 ggttatgcag taaactttgt gaaactgaca cgcgaacagc agctggatgt gatttcaaga
     3061 acaatcaatc attctttgta attgaaagtt ggaaattaaa ggaaacatac attcactaga
     3121 gagtttcggg accgttgatg gtcccggaat tcgttttgtt gtctttatgc agggttgtcc
     3181 gctccgttgc ctgtattgcc acaatccgga tacctgggat agcaaaggtc ttgccaaata
     3241 tcagatgacg cctgaagaat tattggctga ggttttacga tataagaact ttatcgccaa
     3301 gggaggcgtg actgtcaccg ggggagaacc tttattgcaa gctgaatttc tcagggagtt
     3361 tttccgtctt tgccgggaag ccggtattca tacggcgctc gatacatccg gctatatctg
     3421 cacaccgaaa gccctggcgg ttctggaata tgccgacctg gtcttattgg atataaaaac
     3481 catcgatccc gacctgcatc cccgtctaac ggccgtgaaa ctggataata cgttacgttt
     3541 cctggacgaa ctggaaaaac gcggtgttcc ggtttggata cgccatgtca ttgtgcccgg
     3601 actgacggat aatgacgaag ccttggataa actggccgaa tacatcagcc actatacggt
     3661 tgtacagaag gcggaactct tgccttatca cacgatgggc acatataaat acgaggctca
     3721 aggtctcgac tacaaactga aagaggtaga acctttatcg gccgaacgtc tggcaaatgc
     3781 caaagaaata tttaagaagc acggtataac catctgataa tttacctata atcaggtata
     3841 tttttattgc tctaaaaaac atagttttgt cccatattgt tactcaaata aaagtaggaa
     3901 tgaatatgga acaacatcat catgatcatc accaccacga aataacctct ttgaacaagg
     3961 ctttcattat aggtatctcc ctgaacctgg cttttgtcgt agccgaattt acggctggct
     4021 tctggtataa ttcattagga cttctgtcgg atgcagggca taacctggga gatgtggcca
     4081 gcctggtact ggctatgctg gctttccggc tggctaaggt gcacccgact tgcagctata
     4141 cctatggata taagaaaagc actgtactcg tatccctcct gaatgcggtc atcctcttgg
     4201 tggccgtcgg gattattatt gctgaaagta taggaaaatt gctgcatccg cgtccggtcg
     4261 agggtgatgc gatcgcttgg gtggcaggta tcggagtcgt tataaatgca cttacggcat
     4321 ggcttttcat gaaggataag gataaagact tgaatgtgaa aggtgcttac ttgcatatgg
     4381 ctgccgatgc actggtatcg gtcggcgtgg ttgtctccgg aattgttatt gcccataccg
     4441 gatggtatat catcgacccg attatcggcc tgttgattgc gttcgttatc attgtttcta
     4501 cctggggact gctacgcgac agtatccgtc tctctctgga cggtgtgccg gcgggaacgg
     4561 acatcgataa ggtggaaaag acgatacatt cggtcgaagg cgttgaaggc gtccatcatc
     4621 tccatatctg ggcgatcagt acgaccgaaa cggcacttac cgcccatgtt tccgttgcgg
     4681 atatttcaga aatggaacgt atcaaacagg agataaaaca cgaccttcag catgtgggaa
     4741 tccagcatgc tacgctcgaa tttgaaacgt cgggattcaa gtgcgatggg cgctgttgct
     4801 cagactgaaa caggtatatt ataaacctga tttactgata ctcaaagatt ttgtctatga
     4861 ctgcataaaa cctttcgatt ggattttttg ttcttacctc gaatcggcaa cttatctttg
     4921 caccagtaac aaaaacaaaa tagtaataac gattaaaata aaaattctat ggaacctatt
     4981 atcaattcac aagcacccga gtttaaagtt caggcatacc ataacggtag tttcaaaact
     5041 gtaagcagcg acgacatcaa aggcaaatgg gcgattttct ttttctaccc ggctgacttt
     5101 acatttgttt gtccgaccga gttggtagat atggctgaca aatatgacca gttccaggca
     5161 atgggtgtag aagtttactc tgtaagtaca gattcacact tcgtacacaa agcatggcac
     5221 gatgcttctg aaacgattcg taagatcaaa tacccgatgt tggcagaccc gacaggcgta
     5281 ttgagccgtg cattcggtgt aatgatcgaa gaagacggta tggcttaccg cggtacattc
     5341 ctggtaaatc cggaaggaaa gatcaaactg gcagaaatca acgataacgg tatcggccgt
     5401 aacgccgacg aactgcttcg taaagtagaa gctgcccagt tcatcgccga acatccgagc
     5461 gaagtttgcc ccgccaaatg gaaaaaaggt ggcgaaacat tgaaaccgag catcgatctg
     5521 gtcggtaaga tctaatagta aaactggagg ccggcaa
//
