LOCUS       c00186_gnlPro..         4448 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|LCEAGGGN_186.
ACCESSION   c00186_gnlPro..
VERSION     c00186_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 07:17:31
            Original ID  :: gnl|Prokka|LCEAGGGN_186
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4448
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4448
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:4448]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..4448
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..4448
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4448
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             complement(3..701)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: AcetylCoA_hydro"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg186_1"
                     /sec_met_domain="AcetylCoA_hydro (E-value: 1.8e-22,
                     bitscore: 69.5, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VNNGEIAYNDIHLSQMAQEVRYGFMGNVDIAIIEACEVTADGKIY
                     LTAAGGIAPTICRLADKIIVELNAAHSKSAMGLHDVYEPLDPPYRREISIFKPSDRIGK
                     PYIQVDPKKIVGIVETNWPDEARSFAAADPLTDKIGQNVADFLAADMKRGIIPSTFLPL
                     QSGVGNIANAVLGALGRDKTIPAFEMYTEVIQNSVIGLIREGRVKFGSACSLTVTNDCL
                     EGIYRDMDFFR"
     CDS             complement(698..940)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: AcetylCoA_hydro"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg186_2"
                     /sec_met_domain="AcetylCoA_hydro (E-value: 1.3e-13,
                     bitscore: 40.6, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSYKVLTPEEAASLIKHGHHIGLSGFTPAGTAKAVTAALAKIAEA
                     EHVLPLAIPATESFPGPRQSATALPTPPTLISARR"
     CDS             complement(1001..1528)
                     /locus_tag="ctg186_3"
                     /transl_table=11
                     /translation="VHWGHLPLVAGLALFNMLRGMGLDSVRLRWPNDLLAGKSKLAGIL
                     VERPAENMAVIGIGINVFNDVSSIAGEIKDPPARLADLVRDCPSLHAVMASLGSHLNQV
                     LSVFSHGGVPALQEDLSRAWAGERPVSILTDDETVRGIFTGIDDQGNPVLSLPAGTVRT
                     IPAHLINRLLEE"
     CDS             complement(1817..2554)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg186_4"
                     /sec_met_domain="OAD_beta (E-value: 3.7e-102, bitscore:
                     331.0, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LTPAATGFRWYDWIGLGIKAEIFPPIIFLGVGALTDFGPLLAAPR
                     TLLLGAAAQVGVAATFFMALFMGFTPNESASIGIIGGADGPTSIFLTMKLAPHLLGAVA
                     VAAYTYMSLVPLIQPPIMALLTTKKERLIRMKSLRTVSKSEKLFFAVLVTIVTILLIPD
                     ASPLIGMLMLGNFLRECKVTERLVQASQNEIINIVTIFLGTSVGLTMQGDRFLQSETLL
                     IILLGIVIGTAVIAGYYIATLAK"
     CDS             complement(2520..2735)
                     /locus_tag="ctg186_5"
                     /transl_table=11
                     /translation="VRESQEKAKADAARLGDDSLTVDPNLKDFQNVTEDNGNEPVFLLT
                     NGEGTTVVRQQGVNYFDTSGNRIPVV"
     CDS             complement(2704..3051)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg186_6"
                     /sec_met_domain="OAD_beta (E-value: 5.3e-17, bitscore:
                     51.0, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGVFSLSWQMVGMWGIAILLLYLGVAKQYEPLLMVPIAFGALIAN
                     IPDNGMLITQLNQQVISSNEQGEVTATSLNNVGYLRVHVAPLQQAPSKVPANLTTPEDQ
                     VRAGIPGKSKS"
     CDS             complement(3169..3570)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 58.4; E-value:
                     1.6e-18)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg186_7"
                     /sec_met_domain="Biotin_lipoyl (E-value: 2.3e-19, bitscore:
                     58.4, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKLRITVDGKVFDVSVELLDQVSSTTAAPAPAPVAAPAPAASAP
                     VAAPVPAPAPAPAPAAAGAGDVPSPLAGKVVSLDVAAGTPVKAGDQILTLEAMKMNTII
                     YAPASGTLTAFCVNPGDTVQEGQALAKIG"
     CDS             complement(3643..4095)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg186_8"
                     /sec_met_domain="OAD_gamma (E-value: 2e-10, bitscore: 30.5,
                     seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLLTTLAFAFAQGIANVMDNLNYQVVGIMVVMLCLGGMAVILTVS
                     GSIAAAIQNRAKAKTAAPVAVPAAAPAAAGKPGMTPEMLAILSAAVDSAMTELTPEMVA
                     VISAAVDAGLDGMSHRIVEIKQSPGSGYAAAGRAEIFASHRIRPSR"
     CDS             complement(4167..4448)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Vparvula"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg186_9"
                     /sec_met_domain="CT_Vparvula (E-value: 3.8e-27, bitscore:
                     84.3, seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="YIAMCCKDLGADAVFAWPGAEIAVMGAEGAVPVLYGRELKAVEDP
                     AEKAKRQGELLEEYREAFYNPYVAAGMGQITEVXXXPAPKSPSHCAPC"
ORIGIN
        1 tcgcggaaaa agtccatatc cctgtaaatg ccttccaggc agtcgttcgt cacggtcagg
       61 gagcaggcac tgccgaattt cacgcggcct tcccgaatca ggccgatgac ggaattctgg
      121 atgacttccg tgtacatctc gaacgcggga atcgtcttgt cgcggcccag ggcgcccagc
      181 accgcgtttg cgatgttgcc tacgccggac tggagaggca ggaacgtgga gggaatgatg
      241 ccccgtttca tgtcggcagc caggaaatcg gccacgttct gcccgatttt atcggtcagg
      301 ggatccgctg cggcgaagga acgggcttca tccggccagt tggtttccac aatgcccaca
      361 atctttttcg ggtcaacctg gatgtacggc ttgccgattc ggtcgctggg tttgaagatg
      421 gagatttcgc ggcggtaggg aggatccagg ggttcgtaaa cgtcgtgcag gcccatggcg
      481 ctcttgctgt gggcggcgtt taattccacg ataattttgt ctgccaggcg gcagatggtg
      541 ggagcgatcc cgcccgcggc cgtcaggtaa attttgccgt cggccgtgac ttcacaggct
      601 tcaataatgg cgatgtccac gttgcccatg aacccgtagc gcacttcctg ggccatctgg
      661 gacaaatgga tgtcattata ggcgatttcc ccgttgttca ccgccttgcg gaaatcaacg
      721 ttggtggtgt agggagcgcg gtagcggatt gccttggccc gggaaaggat tccgtcgcag
      781 gaatcgccag tggaagcaca tgttccgctt ctgcgatttt tgccagcgca gccgtaacgg
      841 ccttggccgt tccggcgggc gtgaatccgc tcagcccaat atggtggcca tgcttgatca
      901 ggcttgctgc ttcttctggt gtcagtacct tgtaactcat gtcagtgatt tttgttggat
      961 gaaaaaagaa agaaaccccg tgtaaaacgg agcggcaggc tcattcttcg aggagcctgt
     1021 tgatcaggtg cgccggaatg gtgcggacgg tgccggccgg aagggatagc acaggattgc
     1081 cctgatcgtc aatccctgta aaaatgcccc ggacggtttc gtcatccgta agaatggaaa
     1141 ccggcctttc tccggcccat gcccgggaca gatcctcctg aagggcgggg acgccgccgt
     1201 gggaaaagac ggaaagaacc tggttcaaat gactgcccag ggaagccatg acggcgtgca
     1261 gggaagggca gtcccgaaca agatccgcca gccttgccgg agggtccttg atttccccgg
     1321 cgatggagga tacatcattg aaaacattga tgccgatgcc gatgacggcc atgttctccg
     1381 ctgggcgttc caccagtatt cccgccagtt tggatttgcc cgccagcagg tcgttcggcc
     1441 agcgcaggcg cacggaatcc agccccatgc cgcggagcat gttgaacagg gccagccccg
     1501 caaccagggg caaatggccc cagtgcacgc cggccgctgt atcctcagga gcggacaggg
     1561 ggacggtgaa ggatgcccac aggccgcctt cctcccctat ccatttgcgg ttgaacctgc
     1621 cgcggcctcc tgtctgannn nnnnnnnggc aatatgcccc ggggggaggg ctcgggccag
     1681 atcattggtg gaaaaagcct cggcccattc atgataagtc cagtcgccgc atggtccttc
     1741 ctcctccgga ggggtggaaa atgaggctgc ggaattcatg ggacgggccg aggccgggag
     1801 taattttcaa ttctggttat ttcgccagag tggcgatgta gtagcccgcg atgactgcgg
     1861 tgccgatcac gatacccaga aggatgatca gcagcgtttc cgactgcagg aagcggtcgc
     1921 cctgcatcgt gagccccacg gaggttccca ggaagatggt gacgatgttg atgatttcat
     1981 tctgggaggc ctgaaccagg cgttccgtca ccttgcattc gcgcaggaag ttgccaagca
     2041 tcagcatgcc gatcagcggg gatgcgtccg gaatcagcaa aatggtgaca atggttacca
     2101 gcacggcgaa aaacagtttt tcgctcttgc tgaccgttcg cagggatttc atgcggatga
     2161 ggcgttcctt cttggtcgtc agcaacgcca tgatgggcgg ctgtatcagc ggaaccaggg
     2221 acatgtacgt gtatgcggcc acggccacgg cgcccagcag gtgcggggcc agcttcatcg
     2281 tcaggaagat ggaggtgggg ccgtcagcgc cgccaatgat gccgatggac gcggattcat
     2341 tcggggtgaa ccccatgaac agggccatga agaacgtggc ggctacgccc acctgggccg
     2401 ctgcgcccag aagcagggtg cgcggagcgg caagcagcgg gccgaagtcc gtcagggctc
     2461 ctacgcccag aaagatgatg ggcgggaaaa tttcagcctt gatgcccagc ccgatccagt
     2521 cataccaccg gaatccggtt gccgctggtg tcaaagtaat tgacgccctg ctggcggacg
     2581 acggtggtcc cttctccgtt cgtgagcagg aagaccggtt cattgccgtt gtcttccgtc
     2641 acgttctgga aatccttcag gttggggtcc accgtcaggc tgtcgtcgcc cagacgggcc
     2701 gcgtcagctt ttgctttttc ctgggattcc cgcacggact tgatcttcag gcgtggtcag
     2761 gttggccggc accttggagg gagcctgctg aagcggagcc acgtgaacgc gcaggtaccc
     2821 cacgttgttc agagacgtgg cggttacttc cccctgttca ttggaggaga tgacctgctg
     2881 gttcagctgg gtgatgagca ttccgttatc cggaatgttg gcaatgaggg cgccgaaagc
     2941 aatgggaacc atcaggagcg gttcgtattg cttggctaca cccagataca ggaggagaat
     3001 ggcaatgccc cacatcccta ccatctgcca tgatagggag aagacgccca tcccctgtaa
     3061 aaaggtgatt aatgaatcaa aaagactcat agggaatatt tttgtagcgg tttattatct
     3121 gagccggagc ccgggactgt tggcgtttcc gggctgccgc cgccgggatt aaccaatttt
     3181 ggccagtgcc tgtccttcct gaaccgtgtc gcccgggttc acgcagaaag cggtgagcgt
     3241 gccggaggcg ggagcgtaga tgatggtgtt catcttcatg gcttccagcg tcagaatctg
     3301 gtcaccggcc ttcacggggg tgcctgctgc cacgtccagg gatacgacct tcccggcaag
     3361 cgggctggga acgtctcctg cgccggcggc agccggagcc ggagcaggtg cgggagccgg
     3421 gaccggagcg gccacgggtg cggaagccgc aggagcggga gcggccacag gtgcgggggc
     3481 cggcgccgca gtggtggagg acacctggtc cagaagttct acggatacgt caaatacttt
     3541 gccgtctacg gtgatgcgaa gtttcttcat aatagtgtgt ggtatgggat attaagttaa
     3601 atggttccgc ctgggcgggg taagggtgaa taatgcagta gttcagcggg aaggccggat
     3661 gcggtgggag gcgaaaattt ccgccctgcc cgcggccgca tagccggaac ccggggattg
     3721 cttgatttcc acgatgcggt ggctcatgcc gtctaggcct gcgtccacgg cggcggagat
     3781 gacggccacc atttccggcg taagctccgt catggcggaa tccacggcgg cggaaaggat
     3841 ggcgagcatt tccggagtca tgcccggttt gcccgccgca gcgggagcgg cggcaggcac
     3901 ggctacggga gcggctgttt tggctttggc cctgttctga atggcggcgg cgatggagcc
     3961 gctgacggtc agaatgacgg ccatgcctcc caggcacagc ataaccacca tgatgccgac
     4021 aacctgataa ttcagattgt ccataacgtt ggcgatgccc tgtgcgaagg caaaagcgag
     4081 tgttgtaagt aacatatgaa tccgttggga tggatgttag agcggaatgt tgccgtgttt
     4141 cttggccggg cgcacttcct tcttgttcag cagggtgcgc agtgcgaagg cgattttggc
     4201 gcgggnnnnn nnnnnacttc cgtaatctgt cccatgccgg ccgccacata cgggttgtaa
     4261 aaggcttccc ggtattcttc cagaagttcg ccctggcgct tggctttttc cgccgggtct
     4321 tccacagcct tcagttcgcg gccgtagagt accgggacgg cgccttccgc gcccataacg
     4381 gcaatttccg caccaggcca ggcgaagacg gcgtcagcac ccaggtcctt gcagcacatg
     4441 gcgatgta
//
