LOCUS       c00056_gnlPro..         5059 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|JOEEOPDO_56.
ACCESSION   c00056_gnlPro..
VERSION     c00056_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 05:03:40
            Original ID  :: gnl|Prokka|JOEEOPDO_56
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5059
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..732)
                     /locus_tag="ctg56_1"
                     /transl_table=11
                     /translation="MFKSGLAVLFLLASWFSAACAYELLPAHVAVVYNGKSELSRRMAR
                     EYARVRGVPEGNLVSLDCPMTSEISRKEYEDTIRVPLLETARKQRWWVPSGIASSPLMN
                     RKIFVLVLMADLPMKIRHETPAPLPGKGVNQMQTDRAAVDSELALLAVGGYERKSWQVN
                     PYFNKREDFVGSGLPSFLVCRLDGLTPDTCMRLVTEPANVEKKGLWGWAVVDRGGPYAQ
                     GDRWLDDVFERVREAGIPAYLD"
     protocluster    1..5059
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[849:5059]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      850..5059
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..5059
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5059
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             850..1320
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Glyoxalase_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg56_2"
                     /sec_met_domain="Glyoxalase_4 (E-value: 4.3e-26, bitscore:
                     80.1, seeds: 51, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYCRPPPCIPYNHHTHMIQQIDHIGIAVKSLDATVPYYRDALGLG
                     EPHIEEVPTQKVRVAMFDVAGVHIELLEPTSPESAIAKAIEKKGEGIHHIAFKTNDIAG
                     NISQAKEAGLTVLNDPPVPGAHNTQVTFLHPKCTFGVLTEFCQHPGGCECGK"
     CDS             1352..2917
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Vparvula"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg56_3"
                     /sec_met_domain="CT_Vparvula (E-value: 5.5e-217, bitscore:
                     709.8, seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAIDPKLIDDLNSRRKKVILSGGQEKIDKRHEKGEMTARDRMGYL
                     FEEGTFSEIGMHVRHNCHNFGMGKKEIPGDGVVSGFGLVDGRPVACAASDFLAQGGSLG
                     YMHAMKIADAQKYALKAGIPMVTVNDSGGARLQEGVAALSGYAQVFYNNVLASGVVPQI
                     SMILGPCAGGAAYSPALTDFIIMRNSGNAGMYITGPKVIEQVTYEKCTMDDIGSAAIHA
                     TVSGNVHFVADSDAHAMDILKRLLSFLPSNNTEEPPHKLDTPLDLSADEGMSDLIPGDN
                     RTPLDVQPIISRLVDNGDFLEVHKDFAKNVVVGFGRICGVVVGIIANQPNVKAGCLDID
                     SSDKAARFIRFCNAFNIPLVNLVDVPGFLPGKNQERGGIIRHGAKLIFAYSQATVPKVT
                     LIMRKAYGGAYIAMCCKDLGADAVFAWPGAEIAVMGAEGAVPVLYGRELKAVEDPAEKA
                     KRQGELLEEYREAFYNPYVAAGMGQITEVINPEETRAKIAFALRTLLNKKEVRPAKKHG
                     NIPL"
     CDS             2939..3391
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg56_4"
                     /sec_met_domain="OAD_gamma (E-value: 2.5e-10, bitscore:
                     29.7, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLLTTLAFAFAQGIANVMNNLNYQVVGIMVVMLCLGGMAVILTVS
                     GSIAAAIQNRAKAKTAAPVAVSAAAPAAAGKPGMTPEMLAILSAAVDSAMTELTPEMVA
                     VISAAVDAGLDGMSHRIVEIKQSPGSGYAAAGRAEIFASHRIRPSR"
     CDS             3464..3865
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 58.8; E-value:
                     1.2e-18)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg56_5"
                     /sec_met_domain="Biotin_lipoyl (E-value: 1.2e-19, bitscore:
                     58.8, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKLRITVDGKVFDVSVELLDQVSSTTAAPAPAPVAAPAPAASAP
                     VAAPAPAPAPAPAPAAAGAGDVPSPLAGKVVSLDVAAGAPVKAGDQILTLEAMKMNTII
                     YAPASGTLTAFCVNPGDTVQEGQALAKIG"
     CDS             3983..5059
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg56_6"
                     /sec_met_domain="OAD_beta (E-value: 2.7e-74, bitscore:
                     238.9, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGVFSLSWQMVGMWGIAILLLYLGVAKQYEPLLMVPIAFGALIAN
                     IPDNGMLITQLNQQVISSNEQGEVTATSLNNVGYLRVHVAPLQQAPSKVPANLTTPESR
                     AQYQEIMQQPMQVYPGSQLTVSKIKSVRESQEKAKADAARLGDDSLTVDPNLKDFQNVT
                     EDNGNEPVFLLTNGEGTTVVRQQGVNYFDTSGNRIPVDLKTQKLEPLVVSAAGKYVAVG
                     QHTQELLVTSIHGGLYDWIGLGIKAEIFPPIIFLGVGALTDFGPLLAAPRTLLLGAAAQ
                     VGVAATFFMALFMGFTPNESASIGIIGGADGPTSIFLTMKLAPHLLGAVAVAAYTYMSL
                     VPLIQPPIMALLTTKKERL"
ORIGIN
        1 atccagataa gcaggaatgc ccgcctccct cacgcgctcg aacacgtcgt ccagccaccg
       61 gtccccctgg gcatagggac cgccccggtc cacaacggcc cacccccata aacctttttt
      121 ctccacgttc gccggttccg tcaccagccg catgcaggta tcgggcgtca gtccgtccag
      181 gcggcatacc agaaacgaag gaaggccgga acccacaaaa tcctcccttt tattaaaata
      241 gggattcacc tgccaggact tcctttcata accgcccacc gccagcaaag ccagttcgga
      301 atccaccgca gcgcgatccg tctgcatctg gttgacgcct ttcccgggca ggggagcggg
      361 cgtttcatgc cgtatcttca tcggcaggtc cgccatcagc accagcacaa aaatcttccg
      421 gttcatcaag ggggaggacg caatgccgga aggcacccac caccgttgtt tccgcgccgt
      481 ctccagcagc gggacgcgga tcgtatcctc atactccttc cgcgaaatct cgctggtcat
      541 ggggcaatcc agggaaacga gattcccttc cggaacgccg cgcacccgtg catactccct
      601 ggccatgcgg cggctcagct cggattttcc gttataaacc acggccacat gagcgggcag
      661 cagttcatag gcacacgcgg cggaaaacca cgacgccagc agaaataaca cggctaatcc
      721 actcttgaac atgcccctac catacctcat cttccggggc aatcaagcag gtgaaagggg
      781 cggccacgaa ttcgccattt ttccgctttt cgcttcacat cctaaaaaaa tacgctttaa
      841 taactcttga tgtattgcag gcctcccccc tgcattccct ataaccatca cacgcatatg
      901 attcagcaaa ttgaccatat cggcatcgcc gtcaagagtc ttgacgccac cgtcccctat
      961 taccgcgatg cgctcgggct cggagagccc cacattgagg aagttcccac tcaaaaggtc
     1021 cgcgtcgcca tgtttgacgt tgccggagtc cacatcgaac tgctcgaacc cacctctccg
     1081 gaaagcgcta tcgccaaggc tattgagaaa aaaggggaag gcatccacca catcgctttc
     1141 aagaccaatg acatcgccgg aaatatctcc caggccaagg aagcaggcct taccgtgctg
     1201 aacgacccgc ccgtccccgg cgctcacaac acgcaggtca ccttcctgca tcccaaatgc
     1261 accttcggcg tcctgaccga attctgccag caccccggcg gctgcgaatg cggcaagtaa
     1321 cattctccct attcaccata ccactagacc aatggctatt gatcccaaat tgattgacga
     1381 tctgaacagc cgccgcaaga aagtcatcct cagcggcggt caggaaaaga tcgacaagag
     1441 acatgaaaag ggcgaaatga cagcccgcga ccgcatgggg tacctctttg aggaaggcac
     1501 cttctcggaa atcggcatgc atgtgcgcca caactgccac aacttcggca tgggtaagaa
     1561 ggaaatcccc ggcgacggcg tcgtttccgg tttcggcctg gtggacggcc gcccggtagc
     1621 ctgcgcggcc tccgatttcc tggcccaggg cggttccctc ggctacatgc acgccatgaa
     1681 aattgcggat gcccagaagt atgcgctgaa agccggcatc ccgatggtga ccgtgaacga
     1741 ctccggcggc gcgcgcctcc aggaaggagt ggcggccctt tccggatacg cccaggtgtt
     1801 ttacaacaac gtgcttgcct ccggcgtggt tccgcaaatc tccatgatcc tgggcccctg
     1861 cgcgggcggc gcggcctact cccccgccct gacggacttc atcatcatgc gcaattctgg
     1921 caacgcgggc atgtacatca ccggtcccaa agtgattgaa caggtcacgt atgaaaaatg
     1981 cacgatggac gacatcggct ccgccgccat tcacgccacc gtgtccggca acgtccattt
     2041 cgtagcggac agcgacgcgc acgccatgga catcctgaag aggcttcttt ccttccttcc
     2101 ttccaacaac acggaagaac cgccccacaa actggacact ccgctggacc tgagcgctga
     2161 cgaaggcatg agcgacctga ttcccggcga caaccgcacg ccgctggacg tccagcccat
     2221 catcagccgt ctggtggaca acggggactt cctggaagta cacaaggact tcgccaaaaa
     2281 tgtcgtcgtc ggattcggcc gcatctgcgg cgtggtggtc ggcatcatcg ccaaccagcc
     2341 caatgtgaaa gccggctgcc tggatatcga ctcctccgac aaggccgcgc gcttcatccg
     2401 tttctgcaac gcgttcaata ttccgttggt gaacctggtg gacgtgcccg gcttcctgcc
     2461 cggcaagaac caggaacggg gcggcattat ccgccacggc gccaaactta tcttcgccta
     2521 ttcccaggcc acggtgccca aagtcaccct gatcatgcgg aaagcctacg gcggcgccta
     2581 catcgccatg tgctgcaagg acctgggcgc tgacgccgtc ttcgcctggc ctggtgcgga
     2641 aattgccgtt atgggtgcgg aaggcgccgt cccggtactc tacggccgcg aactgaaggc
     2701 tgtggaagac ccggcggaaa aagccaagcg ccagggagaa cttctggaag aataccggga
     2761 agccttttac aacccgtatg tggcggccgg catgggacag attacggaag tcatcaatcc
     2821 ggaagaaacc cgcgccaaaa tcgccttcgc cctgcgcacc ctgctgaaca agaaggaagt
     2881 gcgcccggcc aagaaacacg gcaacattcc gctctaacat ccatcccaac ggattcatat
     2941 gttacttaca acactcgctt ttgccttcgc acagggcatc gccaacgtta tgaacaatct
     3001 gaactatcag gttgtcggca tcatggtggt tatgctgtgc ctgggaggca tggccgtcat
     3061 tctgaccgtc agcggctcca tcgccgccgc cattcagaac agggccaaag ccaaaacagc
     3121 cgctcccgta gccgtgtctg ccgccgctcc cgctgcggcg ggcaaaccgg gcatgactcc
     3181 ggaaatgctc gccatccttt ccgccgccgt ggattccgcc atgacggagc ttacgccgga
     3241 aatggtggcc gtcatctccg ccgccgtgga cgcaggcctg gacggcatga gccaccgcat
     3301 cgtggaaatc aagcaatccc cgggttccgg ctatgcggct gcgggcaggg cggaaatttt
     3361 cgcctcccac cgcatccggc cttcccgctg aactactgca ttattcaccc ttaccccgcc
     3421 caggcggaat catttaactt aatatcccat accacacact attatgaaga aacttcgcat
     3481 caccgtagac ggcaaagtat ttgacgtatc cgtagaactt ctggaccagg tgtcctccac
     3541 cactgcggcg ccggcccccg cacctgtggc cgctcccgct cctgcggctt ccgcccccgt
     3601 ggccgccccg gccccggctc ccgcacctgc gccggctccg gccgccgccg gcgcaggaga
     3661 cgttcccagc ccgcttgccg ggaaggtcgt ctccctggac gtggcagcag gcgcacccgt
     3721 gaaggccggt gaccagattc tgacgctgga agccatgaag atgaacacca tcatctacgc
     3781 tcccgcctcc ggcacgctca ccgctttctg cgtgaacccg ggcgacacgg ttcaggaagg
     3841 acaggcactg gccaaaattg gttaatcccg gcggcggcag cccggaaacg ccaacagtcc
     3901 cgggctccgg ctcagataat aaaccgctac aaaaatattc cctatgagtc tttttgattc
     3961 attaatcacc tttttacagg ggatgggcgt cttctcccta tcatggcaga tggtagggat
     4021 gtggggcatt gccattctcc tcctgtatct gggtgtagcc aagcaatacg aaccgctcct
     4081 gatggttccc attgctttcg gcgccctcat tgccaacatt ccggataacg gaatgctcat
     4141 cacccagctg aaccagcagg tcatctcctc caatgaacag ggggaagtaa ccgccacgtc
     4201 tctgaacaac gtggggtacc tgcgcgttca cgtggctccg cttcagcagg ctccctccaa
     4261 ggtgccggcc aacctgacca cgcctgaatc ccgtgcccag tatcaggaaa tcatgcagca
     4321 gcccatgcag gtttaccccg gcagccagct gaccgtttcc aagatcaagt ccgtgcggga
     4381 atcccaggaa aaagcaaaag ctgacgcggc ccgcctgggc gacgacagcc tgacggtgga
     4441 ccccaacctg aaggatttcc agaacgtgac ggaagacaac ggcaatgaac cggtcttcct
     4501 gctcacgaac ggagaaggga ccaccgtcgt ccgccagcag ggcgtcaatt actttgacac
     4561 cagcggcaac cggattccgg tggatctgaa aacccagaag ctggagcccc tggtggtttc
     4621 cgccgcaggc aaatacgtgg ccgtaggcca gcatacccag gaactgctgg tcacctccat
     4681 ccacgggggc ctgtatgact ggatcgggct gggcatcaag gctgaaattt tcccgcccat
     4741 catctttctg ggcgtaggag ccctgacgga cttcggcccg ctgcttgccg ctccgcgcac
     4801 cctgcttctg ggcgcagcgg cccaggtggg cgtcgccgcc acgttcttca tggccctgtt
     4861 catggggttc accccgaatg aatccgcgtc catcggcatc attggcggcg ctgacggccc
     4921 cacctccatc ttcttgacga tgaagctggc cccgcacctg ctgggcgccg tggccgtggc
     4981 cgcatacacg tacatgtccc tggttccgct gatacagccg cccatcatgg cgttgctgac
     5041 gaccaagaag gaacgcctc
//
