LOCUS       c00212_gnlPro..         2924 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|JOEEOPDO_212.
ACCESSION   c00212_gnlPro..
VERSION     c00212_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 05:03:40
            Original ID  :: gnl|Prokka|JOEEOPDO_212
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2924
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2924
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[151:2924]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      152..2924
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..2924
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2924
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(152..931)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 53.7;
                     E-value: 9e-17)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg212_1"
                     /sec_met_domain="Radical_SAM (E-value: 2.7e-32, bitscore:
                     99.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQVFPQNHDPDGESSVTGLVHSVESCGTVDGPGIRFVLFLSGCS
                     LRCRYCHNPDTSYVRRGRLRSADDVLKEIARYRDFLQAAGGGVTLSGGDPLFQPDFAGA
                     VLKGCRKLGLHTCLDTSGHLGVNAGEEMLADTDLVLLDIKAWNPKRYRALTGGELHPTL
                     EFAERLASLRKPVWLRYVLVPGVTDNLEEIEELSRHAALLGNVERVDVLPFHQLGRFKW
                     DELKLDYTLKDVPEASAELAEQVRGIFRQGGFPDCVH"
     CDS             complement(948..2924)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg212_2"
                     /sec_met_domain="Gly_radical (E-value: 1.9e-46, bitscore:
                     144.3, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 3.2e-257, bitscore:
                     841.7, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="KELEVVVGLQTDAPLKRALMPFGGLRMAQQALESYGFKMCEKTAD
                     IFKKIRKTHNEGVFDAYTSDIRAARSAGIITGLPDAYGRGRIIGDYRRVALYGTDKLIA
                     ERRKDLKNREHSPLTDELIRLREEMSEQIRALEELAQLGASYGCDLTRPAANAREAVQW
                     TYLGYLAAVKEQNGAAMSLGRVSTFFDIYFTRDLEQGLITEEEVQEIIDQFVMKLRIVR
                     FIRTPDYNNLFSGDPTWVTESIGGMGEDGRTLVTRSSFRMLQTLYNLGPAPEPNLTVLW
                     SRNLPEAFKSFCAKVSIETSSVQYENDDLMRPHWGDDYGIACCVSAMRIGKQMQFFGAR
                     ANLAKCLLYALNGGVDELKGKQVAPPSPRYTEEILNYDEVMALYDKMQDWLAKTYIDAL
                     NIIHYMHDKYCYERIEMALHDPEILRTMATGIAGLSVAADSLSAIKYATVKAIRNEEGL
                     IVDFKTEGEFPCYGNNDPRVDDIACSLVSNFMEKLRRLHTYRNSLPTQSILTITSNVVY
                     GKKTGNTPDGRRAGEPFAPGANPMHGRDRNGAVASMLSVAKLSYDDSLDGISYTFSIVP
                     QALGKEERERRVKLVSLLDAYFAATGHHINVNVLERKTLLDAMDHPEKYPQLTIRVSGY
                     AVNFIKLTREQQQEVINRTFHTR"
ORIGIN
        1 gcggaaatgc cgtattccgg agcaattcca gaaatgcgtc ttcctttttg catggattct
       61 gcagtggagg ggagagggcc cctcatccgg aagaggggga tgcctgcgtc cggtttgctg
      121 cggttggtgg cgccaaagcg cgaaaatccg gttaatgcac gcaatccgga aagccgccct
      181 gccggaaaat gccgcggacc tgttccgcca gttctgcgga cgcttccggg acgtccttca
      241 aggtatagtc cagcttcaat tcatcccatt tgaatctccc cagctggtgg aaagggagca
      301 cgtccacgcg ttccacgttg cccagaagcg ccgcatggcg ggagagttcc tcaatttctt
      361 ccagattgtc cgtaacgcca ggtaccagca catacctcag ccacacgggc ttgcggaggg
      421 aggccaggcg ttccgcaaac tccagtgtag ggtgcagttc cccgcctgtc agggcgcgat
      481 accgtttcgg attccatgcc ttgatatcca gcaggaccag atccgtatcc gccagcattt
      541 cttcgcccgc attcacgccc agatggccgg aggtgtccag gcaggtgtgc aggcccagtt
      601 tccggcagcc tttcagcacg gcaccggcaa aatccggctg gaagaggggg tccccgccgg
      661 agagggtgac gccgccgccc gccgcctgca aaaaatcccg gtaacgggct atttccttga
      721 ggacatcatc tgcgctcctg agccggcccc tccgcacgta ggaggtatcc ggattatggc
      781 agtagcggca gcgcaggctg caaccggaca ggaaaaggac gaagcggatg ccggggccgt
      841 ccacggtgcc gcaggattcc acggaatgaa ccaaaccggt gacggagctt tctccgtccg
      901 gatcatggtt ttgtggaaaa acctgcggca tccgcttgca gtccgggtta acgggtgtgg
      961 aaagtgcggt tgatgacctc ctgctgctgt tcccgggtca gcttgatgaa attcacggca
     1021 tagccggaaa cgcggatggt aagctgcggg tatttttccg ggtgatccat ggcgtcgaga
     1081 agcgttttcc gttccagtac gttcacatta atgtggtgac ctgtagcggc aaagtaggcg
     1141 tccagcaggg agacgagctt gacgcggcgt tcacgttctt ccttgcccag ggcctgagga
     1201 acgatggaga aggtgtagga gatgccgtcc agggagtcgt cataggacag cttggctacg
     1261 gagagcatgg aggccacggc tccgttcctg tcccttccgt gcatggggtt ggctccgggg
     1321 gcgaacggtt ccccggctcg gcggccgtcc ggcgtgttgc ccgtcttttt gccgtacacc
     1381 acgttggagg tgatcgtcag gatggactgg gtgggcaggg aattgcggta agtgtggagc
     1441 ctgcgcagtt tttccatgaa attgctgacc aggctgcatg ctatgtcgtc cacgcgcggg
     1501 tcattgttcc cgtaacaggg gaattcccct tccgtcttga agtccacaat cagcccttct
     1561 tcattgcgga tggcttttac ggtggcatac ttgatggcgg acagggagtc cgccgccacg
     1621 gaaagcccgg cgattcccgt agccatcgtg cgcagaattt ccggatcatg cagcgccatt
     1681 tcaatgcgtt catagcagta tttgtcgtgc atgtagtgga tgatgttcag cgcgtcaatg
     1741 taagttttgg cgagccagtc ctgcatcttg tcgtacaggg ccatcacctc atcatagttg
     1801 agaatttcct ccgtgtagcg gggggagggc ggggccacct gcttgccctt gagttcatcc
     1861 acgccgccgt tcagggcgta gagaaggcac ttggcgaggt tggcgcgcgc tccgaagaac
     1921 tgcatctgct tgccgatgcg catggcggac acgcagcatg cgatgccgta gtcgtcgccc
     1981 caatgcgggc gcatcaggtc gtcgttctcg tattggacgg aggacgtttc aatggaaacc
     2041 ttggcgcaga agcttttgaa ggcttccggc aggttccggg accacagaac ggtcaggttc
     2101 ggttccggag ccggccccag gttgtacagg gtctgcagca tgcggaagga actgcgggtg
     2161 acgagggtgc gcccgtcctc tcccatgccg ccgatggatt ccgtcaccca ggtggggtct
     2221 ccggagaaca ggttattgta atcaggcgtg cggatgaaac ggacgatgcg cagcttcatc
     2281 acgaactggt cgatgatttc ctgaacttcc tcttccgtga tgagcccctg ttccaggtcg
     2341 cgggtgaaat aaatgtcgaa gaaagtggaa acgcggccca gggacatggc cgctccattc
     2401 tgttccttca cggcggctag gtagcccagg tacgtccatt gcacggcttc gcgggcgttt
     2461 gcggcggggc gagtgaggtc gcagccgtag gaggcgccca gctgggcgag ttcttccagg
     2521 gcgcgtattt gttcgctcat ttcttcgcgc agacggatca gctcatccgt aaggggggaa
     2581 tgttcacggt ttttgaggtc cttgcggcgt tcggcaatca gcttatccgt gccgtacagg
     2641 gccacccggc ggtagtcccc gatgatgcgg ccgcggccat aggcgtccgg aaggccggta
     2701 ataatcccgg cggaacgggc cgcccggata tcggaagtgt aggcgtcgaa aacgccttca
     2761 ttgtgcgtct tgcgtatctt tttgaaaatg tctgcggttt tctcgcacat cttgaacccg
     2821 taggattcca gggcctgttg ggccatgcgc aagccgccaa agggcatcag ggcccgtttc
     2881 aggggggcgt ccgtctggag gcctacgacc acttccagtt cctt
//
