LOCUS       c00055_gnlPro..         5962 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|DEMIIICM_55.
ACCESSION   c00055_gnlPro..
VERSION     c00055_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 06:19:18
            Original ID  :: gnl|Prokka|DEMIIICM_55
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5962
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5962
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[4382:5960]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      4383..5960
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5962
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5962
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(3..1394)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg55_1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 1.2e-36, bitscore:
                     115.3, seeds: 65, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VLESTLKEQLKGILAGLNANYTFDISISPAYESRQELLDLLGDVA
                     SCSDKISMQVSDGEGLEFILLKDGAKTGIKFRGVPNGHEFTSLLLAILNSDGKGKNFPD
                     ESICNRVKALNGPIHLTTYVSLTCTNCPDVVQALNAMTTLNPQIHHEMVDGAINQAEVD
                     ALKIQGVPSVFADGKLIHVGRGEFGELLSKLEAQYGINESLTEKTVKRYDVVVVGGGPA
                     GASAAIYSARKGLSVAVVAERIGGQVKETVGIENLISVPETTGTQLADNLRLHMRQYPI
                     DLLEHRRIEKVTIEGNEKVLSTAGGEIFKAPAVIVATGASWRKLNVPGEAEYIGRGVAF
                     CPHCDGPFYKGKHVAVVGGGNSGIEAAIDLAGICSKVTVLEFMDELKADQVLQEKAKSL
                     PNVEIFVSSQTTEVVGNGDKVTGIRTKDRKTGEERVIHLDGIFVQIGLAANSGVFKEIV
                     ETNRPG"
     CDS             complement(1414..1980)
                     /locus_tag="ctg55_2"
                     /transl_table=11
                     /translation="MEPSINSQLPEFKVQAFQNGNFKTVTNEDVLGKWAIFFFYPADFT
                     FVCPTELVDMAEKYEQFKSMGVEIYSVSTDSHFVHKAWHDASETIRKIQYPMLADPTGA
                     LSRAFGVMIEEEGMAYRGTFLVNPEGKIKVAEIHDNNIGRDASELLRKVEAAQFVASHD
                     GEVCPAKWKKGEATLKPSIDLVGKI"
     CDS             complement(2201..3061)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MAAL_C"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg55_3"
                     /sec_met_domain="MAAL_C (E-value: 6.9e-08, bitscore: 20.8,
                     seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPVPMMNIINGGSHSDAPIAFQEFMIRPVGAASFKEGLRMGAEVF
                     HALKKVLHDRGLSTAVGDEGGFAPNLEGTEDALNSIIAAIKAAGYEPGKDVMIGMDCAS
                     SEFYKDGIYDYTKFEGEKGVKRTSDEQVDYLEKLINEYPIDSIEDGMSENDWAGWKKLT
                     DRIGNHCQLVGDDLFVTNVEFLSRGIKEGCGNSILIKVNQIGSLTETLNAIEMAHRHGY
                     TTVTSHRSGETEDATIADIAVATNSGQIKTGSLSRSDRMAKYNQLLRIEEELGDRAVYG
                     YKRIN"
     CDS             complement(3166..3498)
                     /locus_tag="ctg55_4"
                     /transl_table=11
                     /translation="MKIEKITGREILDSRGNPTVEVDILLESGVMGRASVPSGASTGEN
                     EALELRDGDKKRYGGKGVLKAVENINTIIAPALKGMSALDQIGIDHAMLALDGTKTKSK
                     LGXXXN"
     CDS             complement(3606..4184)
                     /locus_tag="ctg55_5"
                     /transl_table=11
                     /translation="MKTPQEIAEMISKRFYPLPDADLDSLASIIESSKAAKGELLLDNG
                     QISKYLYYVESGLLRQFYYKNKRDITEHFSCEGNIVICIASTFQREPTRLLIEALEPTV
                     YHRISFSEFSDLLKTSLAINRLYQKILEWALMLSQEKADSWRFESARKRYLRFQQEYPE
                     AAKRASVNHIASYLLMTPESLSRVRAGVL"
     CDS             complement(4383..5117)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 67.6;
                     E-value: 4.7e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg55_6"
                     /sec_met_domain="Radical_SAM (E-value: 9e-34, bitscore:
                     106.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWI
                     RHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPL
                     SKERLDNAKAIFSRYKPENNKA"
     CDS             complement(5118..5960)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg55_7"
                     /sec_met_domain="Gly_radical (E-value: 4.7e-47, bitscore:
                     147.9, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 3.2e-57, bitscore:
                     183.1, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMSLHDGDVER
                     IRATGIAGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVE
                     ITEKFMEYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNG
                     RDTKGAIAALASVAKLPFQHAHDGISYTFAVSPATLGKEREVQINNLVSLLDGYFTPDG
                     GQHLNVNVFDKDLLIDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
ORIGIN
        1 tcaccgggac gatttgtttc gacaatctcc ttaaagacac cactattagc ggctaagccg
       61 atttgaacaa aaataccgtc taaatgaata acacgctctt cccctgtctt acgatccttt
      121 gtacggatac cggttacctt gtcgccattt ccaaccacct cggttgtttg tgaactaaca
      181 aagatctcga cattcggcaa actcttcgct ttctcttgca agacttgatc cgcttttaac
      241 tcatccataa actccaaaac ggttactttc gaacagatcc ccgccaaatc aatagccgcc
      301 tcgataccgg aatttccacc gccaaccacg gcaacatgtt ttcccttata aaacggaccg
      361 tcgcaatgag gacaaaaagc cactccacgc cctatatact ctgcctcacc cggtacattc
      421 aatttacgcc agctagcgcc cgtagcgaca ataaccgccg gagccttgaa tatctcacct
      481 ccagccgtag ataaaacctt ctcgtttcct tctatcgtta ccttctctat acgacgatgc
      541 tccagcaggt caatcggata ttgccgcata tgcaagcgca gattgtcagc caactgggta
      601 cctgttgtct ccggaactga aatcagattc tcaattccta ccgtctcctt cacctgcccg
      661 ccgatacgct ccgccacgac agcgacactc agcccctttc gtgcggaata aatagcggca
      721 gaagcaccag ccggaccccc accgaccaca accacgtcat atcgttttac cgtcttctcc
      781 gttaggctct cattgattcc atactgagct tccagcttcg ataacaattc tccgaattcc
      841 ccacgtccta catggatcag ttttccatcc gcaaagacag aggggacgcc ttgtatcttc
      901 aaggcatcaa cttccgcttg attgatagcc ccatctacca tctcatgatg gatctgagga
      961 tttaaagtcg tcatagcgtt taatgcctgt accacatccg ggcaattcgt acaagtcaaa
     1021 gagacatagg tcgtcagatg tatcgggcca ttcaaggctt taacacgatt acaaatgctc
     1081 tcgtccggaa aattcttgcc tttcccatct gaattcaaga tcgccaacag taaggaagta
     1141 aactcgtgtc cattaggtac tccacgaaac ttaatccccg ttttcgctcc atcctttaac
     1201 aaaatgaatt ccaatccctc cccatcagat acttgcatag atattttatc cgaacaagag
     1261 gcaacatcac ctaataaatc caacaattcc tgtctacttt catacgcagg agagatcgat
     1321 atatcaaagg tataattcgc atttaaccct gcgagtatac ctttcaattg ttcttttaat
     1381 gttgattcta acacgatcta tactcttaat tatttagatt ttccctacca agtcaatgct
     1441 aggtttcaag gtagcctccc ctttcttcca tttagcggga caaacctcac catcatgtga
     1501 agcgacaaac tgagcggctt caactttacg aagtaactcg gaagcgtcac gaccgatatt
     1561 gttatcatga atctcggcca ctttgatctt tccttccgga tttaccaaga aagtgccacg
     1621 gtatgccata ccctcttcct caatcatcac accaaacgca cgactcagtg ctccagtggg
     1681 atccgccaac atcggatatt ggatcttacg gatagtctcg gaagcgtcat gccaagcttt
     1741 atgtacaaaa tgagagtctg tacttacaga gtaaatttct actcccattg atttaaactg
     1801 ctcatacttc tcagccatat cgaccaactc tgttggacaa acaaatgtaa aatcggccgg
     1861 gtagaagaaa aagatagccc acttacccaa gacgtcctcg ttggttacag tcttgaaatt
     1921 accattttga aaagcttgta ctttaaactc cggaagttga gaattaatac tcggttccat
     1981 acttgttcct atttttaaat gactactatt tgtttttatt tccgatacaa aaatataaca
     2041 tatttattca ttaaaacaat ttttctaatc gatatatttt atgaggttat agataatact
     2101 ttatcaatca ataaaaaggg cctatcctca cggataagcc cttcaatatc ttgggttttg
     2161 gattactaca taattagcga ttataattta ctttatatac ttaattgatt cttttgtatc
     2221 cgtatacggc acggtctccc aattcctcct cgatacgaag cagctggtta tatttagcca
     2281 tacggtccga acgacttaat gatcccgtct tgatctgtcc gctattggta gcgacagcga
     2341 tatcggcgat cgtagcgtct tccgtctcac cggaacggtg tgaagtaacg gtcgtgtatc
     2401 catgacggtg agccatctcg atagcgttca gtgtctccgt caaggaaccg atctgattta
     2461 ccttgatcaa gattgagtta ccgcaacctt ctttaatacc ccttgaaagg aactctacat
     2521 ttgtcacgaa caaatcatct ccgactaatt ggcaatggtt accaatacga tctgtcagtt
     2581 tcttccaacc agcccaatca ttctcactca taccatcctc aatggaatcg atcgggtatt
     2641 cgttgatcag cttttccaga taatccactt gttcgtcgga agtacgtttc actccttttt
     2701 ctccttcaaa cttcgtgtaa tcgtagattc catccttata aaactcagaa gaagcgcaat
     2761 ccataccgat catcacatcc ttgcccggtt cataaccagc cgccttgata gcggcgataa
     2821 ttgagttcaa ggcatcttcc gtaccttcca aattcggggc gaaaccaccc tcatcaccaa
     2881 cagcggtact taacccacga tcgtgcaata cctttttcaa cgcatggaat acctcagcgc
     2941 ccatacgtaa gccttccttg aaagaagcgg cacctaccgg acgaatcata aactcttgga
     3001 aagcgatcgg agcgtcgcta tgcgaaccgc cattgattat attcatcatc ggaacaggca
     3061 tcacgtaggt attcgttcca ccaatatatc tatataaagg aatatccaaa taatttgcgg
     3121 cagctttcgc tacagctaaa gaaacgccaa ggatagcgtt cgcacctagt tnnnnnnnnn
     3181 ncctagtttc gatttagttt tcgtaccgtc caaagccaac atcgcatgat cgatacctat
     3241 ctgatccaag gcagacatcc ccttcaatgc cggagcgata atcgtattga tattctcaac
     3301 cgctttcaat acgcctttac caccataacg cttcttatcg ccatcacgca actccaaagc
     3361 ctcgttctcg ccggtggacg caccggaagg cacagacgca cggcccataa cgccggactc
     3421 tagtaaaata tcaacctcta ctgtcgggtt acctcttgaa tctaggatct cacgtcctgt
     3481 aatcttttct attttcattg cttctacgtt ttttaaactg acgcaaatat agatggatta
     3541 cttgaaccgt gaaatcacca caaattgcta ttttcacctc aataatacct gaatgtaggt
     3601 agagactaca acactcccgc ccgcacccgg ctcaaagatt cgggtgtcat caataaatac
     3661 gaggcgatat gattgacaga agcacgtttc gctgcttccg gatattcctg ttgaaaacgc
     3721 agataacgtt ttcttgccga ctcaaaacgc caagagtccg ctttctcttg cgaaagcatc
     3781 aaagcccact ccagtatttt ctggtacaat ctattaatag ctaaagatgt tttcaataaa
     3841 tcgctaaatt ccgaaaaaga gatccggtga taaaccgtcg gttccaatgc ctcgattaat
     3901 aaacgcgtcg gttctctttg gaatgtgctg gctatacaaa taacgatatt tccctcacag
     3961 gagaaatgct cggtaatatc acgtttattc ttatagtaaa actggcgtaa taatcccgat
     4021 tccacataat ataaatattt agatatttgt ccgttatcca acaataattc tcctttcgca
     4081 gccttacttg attctatgat cgaggccaga gaatccaaat ccgcatcggg taatggataa
     4141 aaacgtttgg atatcatttc ggctatttct tgtggtgtct tcatactatt catcattttc
     4201 tgatctatat cattttttat ctccttacaa aaatagatat ttgttgcata taacaaaaca
     4261 aacacatatt aaatagtcaa ggttagtcta ggcaaaaaat tattactatc gtttaaaagc
     4321 taatcggatt agattgtttt ccctgattag ctgttgcata atcgcaaggc ttgatcgaga
     4381 gatcaggcct tgttattttc tggtttgtag cgagagaata tcgctttcgc attatctaaa
     4441 cgttcttttg ataagggttc cacccccttt aacggataat cgagacctaa ttgctcatat
     4501 ttataggttc ccatcgtatg ataaggcaat aattccactt tgcgaaccac tttatatgaa
     4561 ctgacatacc gagctaacgc ctccagccac tcgtcattat ccgtatatcc ggggacaata
     4621 acatggcgaa tccaagtatc tatgccacgt ctctccaatt catccaagaa cagaagcgta
     4681 ttatcctgct tgacacccgc cagtaacgga tgtaaatccg ggtttaaggt tttgatgtcc
     4741 aataacacta agtccgcata atccaagact tcccaagctt ttgaggtaca tacgaaacca
     4801 gatgtgtcca aagctgtgtg aagaccttgc tcttggcaca aacggaaaaa ctctttcaga
     4861 aactcaggtt gcaataaagg ttccccaccg gtaacagtca cccccccacg ggcgataaaa
     4921 ctcttatacc gtaagacttc cgtcaatagc tcgccgggag tcatctgata tttcaccttc
     4981 cctttcgggt tccaagtatc cgggttatgg caatacaggc aacgcaacgg gcagccctgc
     5041 ataaaaacga caaagcgaat tccgggacca tctacggtcc cgaaactctc taatgaatgt
     5101 atcttacctt ttatcactta cagtgaatgg ttaatggttc ttgaaataac atccaactgt
     5161 tgttcacgag tcagtttcac gaagtttacc gcatatccgg atacacggat cgtaagttgc
     5221 ggatatttct caggatgttc catagcgtcg atcaacaaat ccttatcaaa tacgttaaca
     5281 ttcagatgct gacctccatc cggagtaaaa tagccatcca ataagctaac cagattattg
     5341 atctgcactt cacgctcctt tcccaacgta gccggagaaa cagcgaaagt ataagagata
     5401 ccgtcgtgcg catgctggaa tggaagcttc gctaccgaag ctagcgcagc gatagcacct
     5461 ttcgtatcac gaccgttcat agggttcgcg cccggagcga aaggagttcc accgatacgg
     5521 ccatccggtg tcgtaccagt cttcttacca tataccacat tagaggtgat cgtaaggatg
     5581 gactgcgtag gtttcgcatg gcgataggtc tcgtgctgac gcaaatattc catgaacttc
     5641 tcggtgatct ctacagcgat actatccgta cgatcgtcgt tattaccgaa aggaacatat
     5701 tcgccctcac gttcgaaatc aaccgccaag ccacgctcgt cacggatcac acgtactttc
     5761 gtatcacgga tagcagccaa cgaatcggca acgatagaaa gaccggcgat acctgtcgca
     5821 cggatacgct ctacatctcc atcgtgaagc gacatctcga atgcctcata tgcgtattta
     5881 tcatgcatat aatggattat cttcaaggcg ttcacataca ctttcgccaa ccagcgcatc
     5941 atttgctcat acttagccat ca
//
