LOCUS       c00373_gnlPro..         3082 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|DEMIIICM_373.
ACCESSION   c00373_gnlPro..
VERSION     c00373_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 06:19:18
            Original ID  :: gnl|Prokka|DEMIIICM_373
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3082
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..1074
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg373_1"
                     /sec_met_domain="FAD_binding_2 (E-value: 6.6e-16, bitscore:
                     46.4, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 1.1e-39,
                     bitscore: 123.6, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="DGRIWVPKKLEDAKALQAGTKKGKDIPEADRDYYLERRYPAFGNL
                     VPRDVASRAAKERCDAGFGVNSTGLAVFLDFSDAINRLGKEVVKQKYGNLFDMYEEITN
                     DNPYETPMMIYPALHYSMGGLWVDYELMTSIPGLFAIGEANFSDHGANRLGASALMQGL
                     ADGYFVLPYTIQNYLSDQIQVPRFSTDLPEFVEAEKAIKDRIQKLMNVKGKETVDTIHR
                     KLGHIMWEHIGMARDAKGLQEAIQMLKDLKKEFWSNVFIPGNADNLNTELEKALRVADF
                     IEIGTLMAHDALDRAESCGGHFRTEHQTEEGEALRHDDKFMYVSCWEYQGEDKDPVMLK
                     EDLNYEFVVPQTRNYKK"
     protocluster    1..3082
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:1860]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..1860
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..3082
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3082
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             1105..1860
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg373_2"
                     /sec_met_domain="Fer2_3 (E-value: 4.5e-27, bitscore: 82.5,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 8.9e-13, bitscore: 36.8,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 9.5e-07, bitscore: 17.5,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKDISVTLKVWRQRGPKEKGQFETYKIEKINQSVSFLEMLDILN
                     EQLVNEGKEPIVFDHDCREGICGMCSLYVNGHPHGPATGATTCQLYMRRFHDGETITIE
                     PWRSAGFPVIRDLMVDRYAYDKIIQAGGFTSVNTGGVPDANAIPIPKKDADLAMDAAAC
                     IGCGACAAACKNGSAMLFVSAKVSQLALLPQGKVEAARRAKAMVAKMDELGFGNCTNTR
                     ACEMECPKNISVSNIARLNREFICAKLQD"
     CDS             complement(1897..2007)
                     /locus_tag="ctg373_3"
                     /transl_table=11
                     /translation="MKKPRCKYTDFSIPVKGYNAKLCFTTYNKNKKAPFF"
     CDS             2283..3080
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Radical_SAM"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg373_4"
                     /sec_met_domain="Radical_SAM (E-value: 6.1e-17, bitscore:
                     50.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKKRTTSSFRAMSTERKLESIFLFVTGRCNAKCAMCFYAQEMDK
                     KQPDLTFDEIKKISETAGQFNRLWISGGEPTLREDLPEILELFYKNNHIKDVNIPTNGL
                     KPARIVEWVKRFRQNCPDCNINVSLSLDGFGTTHDTQRGVPGNFYKALETLRKIQENFG
                     DDGKVLKNIATVITKYNIDQVEDFMTWVYGRFRTSTHTIEAARGMTRDDGVKILTESTL
                     RKIQDDISPIYSAYADRMVADTTGLRKPITRFFYLGLIRTLYNV"
ORIGIN
        1 gacggccgta tctgggttcc gaagaaactg gaagacgcta aagctctaca ggccggaacg
       61 aagaaaggta aagatattcc cgaggcagac cgtgattatt atttggagcg tcgttatccg
      121 gcattcggaa acttggttcc tcgcgacgtg gcttctcgtg ccgctaaaga gcgttgcgac
      181 gcaggtttcg gcgtgaacag cacgggcttg gccgtattct tggatttctc cgacgcgatc
      241 aatcgcttgg gtaaagaggt cgttaagcag aaatacggta acttgttcga tatgtacgaa
      301 gagatcacga acgataatcc atacgagact ccgatgatga tctacccggc attgcactac
      361 tcaatgggtg gattgtgggt tgactatgag ttgatgacct ccattccggg tctgttcgct
      421 atcggtgagg caaacttctc cgatcacgga gcgaaccgtt taggtgcttc cgctttgatg
      481 cagggattgg ccgatggtta tttcgtatta ccatacacga tccagaacta tttgtccgac
      541 caaatccaag ttcctcgctt cagcacggat ctgccggagt tcgtcgaggc agaaaaggcc
      601 atcaaggatc gtatccaaaa attgatgaac gtcaagggta aagaaaccgt agacactatc
      661 caccgcaaac taggtcatat catgtgggaa catatcggta tggcacgcga cgcaaaaggt
      721 ttgcaagaag ctatccagat gctgaaagac ttgaagaaag agttctggag caacgtattc
      781 atcccaggca acgcagacaa cttgaatacc gagttagaga aagcattacg tgtagccgac
      841 tttatcgaga tcggtacatt gatggcacac gatgctttgg accgcgctga gtcttgtggt
      901 ggtcacttcc gtacggagca ccagacagaa gaaggtgagg ccttgcgtca tgacgataag
      961 ttcatgtacg tatcttgctg ggagtaccaa ggcgaggaca aagatcctgt catgttgaaa
     1021 gaggatttga actatgagtt cgttgttcct caaacacgta actacaagaa gtaataatca
     1081 tcaatctcag aaaaaattag aatcatggat aaagatataa gtgtaacact taaggtttgg
     1141 cgtcagagag gtccgaagga gaaaggacag ttcgagacat acaaaattga gaagatcaat
     1201 cagagtgtct ctttcttgga aatgttggat atcttgaacg agcagttggt caacgagggt
     1261 aaggaaccga tcgtattcga tcacgactgc cgcgagggta tctgtggcat gtgttcattg
     1321 tatgtaaacg gacacccgca cggaccggca actggagcta cgacttgcca gttatatatg
     1381 cgtcgtttcc acgatggaga gacgatcacg atcgaaccgt ggcgttccgc aggtttccct
     1441 gttatccgcg acttgatggt agaccgctac gcttacgaca aaatcatcca agcgggtggt
     1501 ttcacatctg tcaacacagg tggtgtaccc gacgctaatg cgatacctat cccaaagaag
     1561 gacgcagact tagccatgga cgctgccgct tgtatcggtt gtggcgcttg tgctgccgct
     1621 tgtaagaatg gttccgctat gttattcgta tccgctaagg ttagccagct ggctttgttg
     1681 cctcaaggta aagtcgaggc tgctcgccgt gccaaagcta tggtcgctaa aatggatgag
     1741 cttggcttcg gtaactgtac gaacacacgt gcttgcgaga tggaatgtcc gaagaatatc
     1801 tccgttagca acatcgctcg tttgaaccgc gagttcattt gcgctaaatt acaagactaa
     1861 tatttgatta gtatatcatg gaaaaggcgt ctttctctaa aagaaaggcg cctttttgtt
     1921 tttgttatat gtcgtaaaac ataatttagc attatagccc ttgacaggaa ttgaaaagtc
     1981 cgtatatttg catcgtggtt ttttcataat agtattagat ttaaggttaa caaagtttgg
     2041 ttagggcttg tcgtgagaca ggctttaatt gtttatagac ctacctttta aacatggacc
     2101 ataaaatact gcaaatagag attgtttagc gtcattttat cgtcgatctt tgcctttaaa
     2161 gatcatatag tatatgctaa taagtatttt atttggcttc ttatgttatt ttctccgggt
     2221 gttgatcaaa cacaaggaaa aaatcatgcg ccagataaga caaatggtaa aacaaaaata
     2281 aaatggacaa aaaacgaacc accagttctt tcagggcaat gagcacggaa agaaaactcg
     2341 agtcgatctt ccttttcgta acaggccgat gcaacgccaa atgcgcgatg tgtttctatg
     2401 cccaagaaat ggataagaag caacccgacc tcactttcga tgaaatcaag aagatatcag
     2461 aaaccgccgg gcaattcaac cgattatgga tatccggagg tgagcctacc ctgcgtgagg
     2521 atctaccgga aatattggag ttattctaca aaaacaacca tatcaaagat gtgaacatcc
     2581 ctacgaacgg cttaaaacca gcccggatcg tcgaatgggt aaaacggttc cgccagaact
     2641 gcccggattg taatattaac gtaagccttt ccttggatgg tttcggtacg acccacgata
     2701 cacagagagg cgtaccggga aacttctata aggccttgga aaccctgcgt aagatacagg
     2761 agaatttcgg agacgacggc aaggtcctga aaaatatagc aaccgttatc acgaaataca
     2821 atatagacca ggtagaagac ttcatgactt gggtatacgg acgtttccgc acctccacgc
     2881 atacgattga ggccgccaga ggcatgaccc gtgacgatgg cgtgaaaatc ctaaccgagt
     2941 caacgctacg caaaatacaa gatgacatat cccctatcta ttctgcttat gcggatcgga
     3001 tggtagcgga taccaccggc ttaaggaaac cgatcacacg cttcttctat ctcggcctta
     3061 tacgtacctt atataatgtg cg
//
