LOCUS       c00259_gnlPro..         4999 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|OGNFCOMF_259.
ACCESSION   c00259_gnlPro..
VERSION     c00259_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 06:16:17
            Original ID  :: gnl|Prokka|OGNFCOMF_259
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4999
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4999
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1230:4210]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1231..4210
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4999
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4999
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(2..82)
                     /locus_tag="ctg259_1"
                     /transl_table=11
                     /translation="MEPIINSQAPEFKVQAYHNGSFKTVSS"
     CDS             complement(243..1145)
                     /locus_tag="ctg259_2"
                     /transl_table=11
                     /translation="MEQHHHDHHHHEITSLNKAFIIGISLNLAFVVAEFTAGFWYNSLG
                     LLSDAGHNLGDVASLVLAMLAFRLAKVHPTCSYTYGYKKSTVLVSLLNAVILLVAVGII
                     IAESIGKLLHPRPVEGDAIAWVAGIGVVINALTAWLFMKDKDKDLNVKGAYLHMAADAL
                     VSVGVVVSGIVIAHTGWYIIDPIIGLLIAIVIIVSTWGLLRDSIRLSLDGVPAGTDIDK
                     VEKTIHSVEGVEGVHHLHIWAISTTETALTAHVSVADISEMERIKQEIKHDLQHVGIQH
                     ATLEFETSGFKCDGHCCSD"
     CDS             complement(1231..1962)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.3;
                     E-value: 1.6e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg259_3"
                     /sec_met_domain="Radical_SAM (E-value: 3.7e-37, bitscore:
                     116.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LEIKGNIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWDS
                     KGLAKYQMTPEELLAEVLRYKNFIAKGGVTVTGGEPLLQAEFLREFFRLCREAGIHTAL
                     DTSGYICTPKALAVLEYADLVLLDIKTIDPDLHPRLTAVKLDNTLRFLDELEKRGVPVW
                     IRHVIVPGLTDNDEALDKLAEYISHYTVVQKAELLPYHTMGAYKYEAQGLDYKLKEVEP
                     LSAERLANAKEIFKKHGITIW"
     CDS             complement(1970..4210)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg259_4"
                     /sec_met_domain="Gly_radical (E-value: 1.1e-45, bitscore:
                     142.8, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 3.4e-285, bitscore:
                     935.1, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENENWAQFKGEIWKREINVRDFIQNNYKPYEGDDSFLKDSSD
                     KTKKVWDKLTEMFKVEREKGVYDAETKYPQGIDTYGPGYIDKENEVIVGLQTDAPLKRG
                     IYPKCGTRMVENSLEAYGYKLDPMTKEIFTKYRKTHNEGVFSAYTDEMLAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTEILIAEKKHYLKRLDIQELTEEIIQSREEISEQIKAL
                     KAFEKMCAGYGFDVTKPAKDAREAVQFVYLGYLAAVKDQDGAAMSLGRTSTFLDIYIEK
                     DIREGKLTEEEAQELIDQFIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTKTSYRYLHTLYNLGPAPEPNLTVLWFNNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQYFGARANLAKCLLYAINGGRDERSGVQVAPRFEPITS
                     EYLDYNEVMEKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMALHDGSVERIRATGI
                     AGLSIVADSLAAIRDTKVKVIRDERGLAVGFEREGDYVPFGNNDDRTDSIAVDITEKFM
                     EYLRQHQTYRNATPTQSILTITSNVVYGKKTGATPDGREGGTPFAPGANPMNGRDTKGA
                     VAALASVAKLPFQHAHDGISYTFAVSPATLGKERDIQIANMVGLLDGYFTPDGGQHLNV
                     NVFDKELLLDAMEHPENYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
ORIGIN
        1 cgctgcttac agttttgaaa ctaccgttgt ggtatgcctg aactttaaac tcgggtgctt
       61 gtgaattgat aataggttcc atagaatttt tattttaatc gttattacta ttttgttttt
      121 gttactggtg caaagataag ttgccgattc gaggtaagaa caaaaaatcc aatcgaaagg
      181 ttttatgcag tcatagacaa aatctttgag tatcagtaaa tcaggtttat aatatacctg
      241 tttcagtctg agcaacagtg cccatcgcac ttgaatcccg acgtttcaaa ttcgagcgta
      301 gcatgctgga ttcccacatg ctgaaggtcg tgttttatct cctgtttgat acgttccatt
      361 tctgaaatat ccgcaacgga aacatgggcg gtaagtgccg tttcggtcgt actgatcgcc
      421 cagatatgaa gatggtggac accttcgacg ccttcaaccg aatgtatcgt cttttccacc
      481 ttatcgatgt ccgttcccgc cggcacaccg tccagagaga gacggatact gtcgcgcagc
      541 agtccccagg tagaaacaat gataacgatc gcaatcaaca ggccgataat cgggtcgatg
      601 atataccatc cggtatgggc aataacaatt ccggagacaa ccacgccgac cgataccagt
      661 gcatcggcag ccatatgtaa gtaagcacct ttcacattca agtctttatc cttatccttc
      721 ataaaaagcc atgccgtaag tgcatttata acgactccga tacctgccac ccaagcgatc
      781 gcatcaccct cgaccggacg cggatgcagc agttttccta tactttcagc aataataatc
      841 ccgacggcca ccaagaggat gaccgcattc agaagggata cgagtacagt gcttttctta
      901 tatccatagg tatagctgca agtcgggtgc accttagcca gccggaaagc cagcatagcc
      961 agtaccaggc tggctacatc tcccaggtta tgccctgcgt ccgacaaaag tcctaatgaa
     1021 ttataccaga agccagccgt aaattcggct acgacaaaag ccaggttcag ggaaatacct
     1081 ataatgaaag ccttgttcaa agaggttatt tcgtggtggt gatgatcgtg atgatgttgt
     1141 tccatattca ttcctacttt tatttgagta acaatatggg acaaaactat gttttttaga
     1201 gcaataaaaa tatacctgat tataggtaaa ttaccagatg gttataccgt gcttcttaaa
     1261 tatttctttg gcatttgcca gacgttcggc cgataaaggt tctacctctt tcagtttgta
     1321 gtcgagacct tgtgcctcgt atttataagc gcccatcgtg tgataaggca agagttccgc
     1381 cttctgtaca accgtatagt ggctgatgta ttcggccagt ttatccaagg cttcgtcatt
     1441 atccgtcagt ccgggcacaa tgacatggcg tatccaaacc gggacaccgc gtttttccag
     1501 ttcgtccagg aaacgtaacg tattatccag tttcacggcc gttagacggg gatgcaggtc
     1561 gggatcgatg gtttttatat ccaataagac caggtcggca tattccagaa ccgccagggc
     1621 tttcggtgtg cagatatagc cggatgtatc gagcgccgta tgaataccgg cttcccggca
     1681 aagacggaaa aactccctga gaaattcagc ttgcaataag ggttctcccc cggtgacagt
     1741 cacgccaccc ttggcgataa agttcttata tcgtaaaacc tcagccaata attcttcagg
     1801 cgtcatctga tatttggcaa gccctttgct atcccaggta tccggattgt ggcaatacag
     1861 gcaacggagc ggacacccct gcataaagac aacaaaacga attccgggac catcaacggt
     1921 cccgaaactc tctagtgaat gtatgtttcc tttaatttcc aactttcaat tacaaagaat
     1981 gattgattgt tcttgaaatc acatccagct gctgttcgcg tgtcagtttc acaaagttta
     2041 ctgcataacc cgatacacgg attgtcaact gcggatagtt ttccggatgc tccatagcat
     2101 cgagcaacag ctccttgtcg aatacgttca cattcagatg ctgtccgccg tcaggagtaa
     2161 agtaaccgtc cagcaggccg accatattgg cgatctgaat atcacgttct tttcctaacg
     2221 ttgcaggaga aacagcgaat gtataagaga taccgtcgtg tgcatgctgg aacggcagtt
     2281 tagcaacaga agccagtgca gcaacagcgc ctttggtatc acgtccgttc atcgggttcg
     2341 cacccggagc aaacggcgta ccaccttcgc gaccgtcggg agttgcacct gtcttctttc
     2401 cgtatacgac attagacgta atagtcagga tagactgtgt cggagttgca ttacggtatg
     2461 tctggtgctg acgcagatat tccatgaact tttcagtgat gtcgacagca atactatctg
     2521 tacgatcgtc attgttaccg aaaggaacat aatcgccttc acgttcgaaa ccgacagcca
     2581 ggccacgttc gtcgcggatc acttttactt tcgtatcgcg gatagcagcc aatgagtcgg
     2641 caacgataga aaggccggca atacctgttg cgcggatacg ttctaccgaa ccgtcatgca
     2701 aagccatctc gaatgcttca tacgcatatt tatcgtgcat gtaatggatg atcttcagtg
     2761 cgttcacata aactttagcc agccagcgca tcatctgttc gtatttctcc atcacttcgt
     2821 tgtaatccag gtattcgctg gtgatcggtt caaacctcgg agccacctgt acacctgatc
     2881 tttcatcgcg cccgccgttg atggcataca gcaaacattt agccaggttg gcacgtgcgc
     2941 cgaaatactg catctgctta ccgatcttca tcggagacac gcagcaggca ataccataat
     3001 catctccata atccggacgc atcagatcgt cgttctcata ctggatagcg gaagtatcga
     3061 tagaaacctt cgcacagaaa cgtttccagt tttcaggagc gttgttgaac cacagtaccg
     3121 ttaagttggg ttccggtgcc gggccgaggt tatataatgt atgcagataa cggtaagatg
     3181 tcttcgtcac caatgaacgg ccgtcgacac cctgtccgcc caaagactcg gttacccaaa
     3241 cagggtcacc ggagaacagg tcgttatatt ccggagtacg aaggaaacgg acgatacgca
     3301 gtttgataat aaactggtcg atcagctcct gagcttcttc ttccgtcagc ttgccttcgc
     3361 ggatatcttt ttcaatataa atatcgagga aagtagaagt acgtcccaac gacatggcag
     3421 ccccatcctg gtccttcacc gcagccagat aacccagata gacaaactga accgcttcgc
     3481 gtgcatcctt tgcaggcttg gtcacgtcga aaccataacc ggcacacatc ttttcgaaag
     3541 ccttcagcgc cttgatctgt tcggatattt cctcacggct ctggatgatc tcttcggtca
     3601 gttcctgaat atcgagtctt ttcagataat gtttcttttc agcgatcagg atttccgtac
     3661 cgtaaagtgc tacacggcgg tagtcaccga taatacgtcc gcgtccgtaa gcatccggca
     3721 ggccggtgat gatagcggaa cggcgcgctg caagcatttc atctgtataa gcggagaata
     3781 cgccttcgtt atgcgtcttt ctgtatttcg tgaagatttc ttttgtcatc gggtccagtt
     3841 tgtagccata tgcttccaga ctgttttcca ccatgcgggt accacatttc gggtaaatac
     3901 cgcgtttcag gggggcatcg gtttgcaggc cgacaattac ttcgttttct ttgtcaatat
     3961 aacctggtcc gtaagtatct ataccttgag gatattttgt ttcagcgtcg taaacaccct
     4021 tctccctttc aaccttaaac atttcggtca atttatccca aactttcttc gtcttatctg
     4081 aggagtcttt caagaaagag tcgtcccctt cgtaaggttt gtagttgttc tggataaaat
     4141 cccttacgtt aatctctctt ttccaaattt cgcctttgaa ctgcgcccag ttctcgtttt
     4201 caaacttcat accttatttt gttttcttcg gttttaattt cggtgcaaag gtacgttatt
     4261 tatatggcat gcgaaacaat caacaaaaac atcatgtatt cccaacggcc aaaatcccta
     4321 caaatagtta ctaaaacggt agatatagtg cagcgtatcg cctgatatag gtatcaaaca
     4381 ggaaggatta taaataatat caaaaaaata aaaaaggaac catctgtcgc caaatgatcc
     4441 cgaatcgtct ctgtagctct tatttctccg ttacggtgct gtgtgcactg tcatatacnn
     4501 nnnnnnnnac catccttcgc aggagagtcc cttttttatg ccggaaacaa ccaataaaaa
     4561 atttccgaca cacgtttaat catcttatat gcttacataa ttagcaaaag gttttgatat
     4621 agcgattata ctaatagtgt tacttttttc tatgttgcaa agatagagcc gtcaagggga
     4681 gtacacaata cctattatat agtaaaaaaa gagcttccta ctacctattc tgtgttataa
     4741 tcgggaaaat tgatcattgg aataaacaat atttgcaaaa attgctttct ttgataaata
     4801 aaaaagagcc attcttcaca ggatggctcc cttttatata tgaaagggac aaccaataaa
     4861 aaaactccct tccatgggta tttaccttaa ataataggtg tacataaaaa tcaacaaatc
     4921 ggattttggt caggacgatt tatactaata gtaaaactta attgatacgg caaaggtaaa
     4981 accacggaaa ggcacaggc
//
