LOCUS       c00032_gnlPro..         7541 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|FHKDPNIH_32.
ACCESSION   c00032_gnlPro..
VERSION     c00032_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 11:30:15
            Original ID  :: gnl|Prokka|FHKDPNIH_32
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7541
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..2439
                     /locus_tag="ctg32_1"
                     /transl_table=11
                     /translation="SMDSLLAVLSEKDPHRATAVMYYSWITEKPFLNHSLLYSTLYQGI
                     NGISRHPVFSLYDMGVEEGYTIGGYYNSAKTIETALIPLLQQVYNGEDMGKIPVSTVDD
                     PHKYLNYVSLISAISNEDNFPRDAIYLNAPPSFLEKYWMQLLGFLIFFLVVVFLAWHYV
                     YRSKQKMKEVELRLLSRYRDLFNNMPLPYIRQRLIREGTQIDVQVLDVKHAFEEKIAPK
                     DFVVNKHGKEIANLIGGSYPLLLSAVPTVLESGKSFTCEYYFEPTGLYYTIIIMPTSEE
                     NVVDAFFIDITDIHKFQTHLKAMNHKLAMALEAADLLPWRYNLAEGKIIYESKVHPGDN
                     IETAEVHTHEVSLKEYFSKIHPSFRACVEKAFDDLCNGKIKKVRKEYCLERLIPGEDRH
                     EWEEIQVMAEYDASGKPKALIGSTISITERKQLEHDLRMARDKAEESNKLKSAFLANMS
                     HEIRTPLNAIVGFSNILASTDDLEDKKEFADIIENNNSLLLQLINDILDLSKIEAGTLE
                     FTYDYVDINAVLRDLEQSAHLKNKNPDVQISFKDYLEPCVIYTDRNRLSQLMINLLNNA
                     MKFTQAGSILFGYKLRDDNTLWFYVEDTGCGIPENKRKDIFGRFVKLNTFAQGTGLGLS
                     ICEMIVTQMKGTIGVDSVEGKGSRFWFTLPFQPKKELLPNEEQKPVTLEKIARSEVTIL
                     IAEDNSSNYRLFESILKPEYKLIHAWNGKEAVELFHQHKPQLILMDIKMPVMDGYEATA
                     EIRKVSASVPIIAVTAYAFAQDEQRIINSGFDAYTAKPINGKILKDKISTLLTHRIILM
                     "
     protocluster    1..7541
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[5544:7539]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      5545..7539
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..7541
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7541
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             2538..2630
                     /locus_tag="ctg32_2"
                     /transl_table=11
                     /translation="MKKPRCKYTELLLFVNGLSAKLCFVTCNKI"
     CDS             complement(2645..3877)
                     /locus_tag="ctg32_3"
                     /transl_table=11
                     /translation="MKILLVTRGSQGDVLPYLAIAAELERRGHKVTINLPQIFEETVKP
                     YGFKYVLQQFDDIGGMIDSAAQNSHKFRPFLKWMRNVIDKQFDQLIPLLKEHDILVSTN
                     SEFAVASIAEYCKKPLIRTAYAPFLPGKKIPPAVLPFPKPNPIITPAILWKLMNRMTNF
                     MVKDTINKNRAKYGLAPIRNFGYHAGERSYNYLLFSQHLGNVDPDWTFEWSIGGYCFND
                     TFQYDEKAYEEMISFVDSAQSPIIFFTLGSCSSKDGNRFSKALVDICKKHDYRLIIGSG
                     WAKTGITLQADKHLFLMKQPVPHNLIFPHCDGVIHHGGCGTTHSVGRAGKPQLITPLII
                     DQPYWSYRIHQLGLGPEGVKIAKASEQEIERKVCDLVTNPLYKKNAAHIGEMIQKEGGI
                     KNICDSIERFQ"
     CDS             complement(3911..5122)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Radical_SAM"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg32_4"
                     /sec_met_domain="Radical_SAM (E-value: 3.1e-16, bitscore:
                     49.1, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKKRTTSSFRAMSTERKLESIFLFVTGRCNAKCAMCFYAQEMDK
                     KQPDLTFDEIKKISETAGQFNRLWISGGEPTLREDLPEILELFYKNNHIKDVNIPTNGL
                     KPARIVEWVKRFRQNCPDCNINVSLSLDGFGTTHDTQRGVPGNFYKALETLRKIQENFG
                     DDGKVLKNIATVITKYNIDQVEDFMTWVYGRFRTSTHTIEAARGMTRDDGVKILTESTL
                     RKIQDDISPIYSAYADRMVADTTGLRKPITRFFYLGLIRTLYNVRASNIDHPTPWGMDC
                     TAGETTLVIDYDGRFRACELRKPLGNIKEYGCDISKVMNSEAMKQEIAAIGHGYKANCW
                     CTHGCWITSSVIFNPRKMIRSVYKGYRETKRLNHPLAINEQKLQTMEAKYHLDIERLRQ
                     LNIR"
     CDS             5398..5508
                     /locus_tag="ctg32_5"
                     /transl_table=11
                     /translation="MKKPRCKYTDFSIPVKGYNAKLCFTTYNKNKKAPFF"
     CDS             complement(5545..6300)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg32_6"
                     /sec_met_domain="Fer2_3 (E-value: 7.8e-27, bitscore: 82.5,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 1.6e-12, bitscore: 36.8,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 1.7e-06, bitscore: 17.5,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKDISVTLKVWRQRGPKEKGQFETYKIEKINQSVSFLEMLDILN
                     EQLVNEGKEPIVFDHDCREGICGMCSLYVNGHPHGPATGATTCQLYMRRFHDGETITIE
                     PWRSAGFPVIRDLMVDRYAYDKIIQAGGFTSVNTGGVPDANAIPIPKKDADLAMDAAAC
                     IGCGACAAACKNGSAMLFVSAKVSQLALLPQGKVEAARRAKAMVAKMDELGFGNCTNTR
                     ACEMECPKNISVSNIARLNREFICAKLQD"
     CDS             complement(6331..7539)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 63.2; E-value:
                     7.8e-20)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg32_7"
                     /sec_met_domain="FAD_binding_2 (E-value: 8.8e-21, bitscore:
                     63.2, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 2.4e-39,
                     bitscore: 123.2, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="SAAWQCYKKGAYFANPCMVQIHPTCVPVKGDFQSKLTLMSESLRN
                     DGRIWVPKKLEDAKALQAGTKKGKDIPEADRDYYLERRYPAFGNLVPRDVASRAAKERC
                     DAGFGVNSTGLAVFLDFSDAINRLGKEVVKQKYGNLFDMYEEITNDNPYETPMMIYPAL
                     HYSMGGLWVDYELMTSIPGLFAIGEANFSDHGANRLGASALMQGLADGYFVLPYTIQNY
                     LSDQIQVPRFSTDLPEFVEAEKAIKDRIQKLMNVKGKETVDTIHRKLGHIMWEHIGMAR
                     DAKGLQEAIQMLKDLKKEFWSNVFIPGNADNLNTELEKALRVADFIEIGTLMAHDALDR
                     AESCGGHFRTEHQTEEGEALRHDDKFMYVSCWEYQGEDKDPVMLKEDLNYEFVVPQTRN
                     YKK"
ORIGIN
        1 tccatggata gtttattggc ggttctgtcg gagaaagatc cgcatcgagc gacagccgtg
       61 atgtattact cgtggattac cgagaaacct ttcttgaatc actcgttatt atatagtact
      121 ttatatcaag gtataaacgg tatttctagg catcctgttt tttctttata tgatatgggt
      181 gtggaagaag gatatacgat tggtggatat tataatagcg ctaaaacgat tgagactgct
      241 ctaatcccct tgctacaaca agtttataac ggtgaagata tgggcaagat cccggtttca
      301 acggtggatg atcctcataa atacttgaat tatgtttcgt tgataagtgc gatatctaat
      361 gaagataatt tcccgcgtga tgcgatctac ttgaatgcgc ctccctcttt cttggaaaaa
      421 tattggatgc aactgttagg ctttctgata ttctttctgg tagtcgtgtt tttagcgtgg
      481 cattacgtgt atcgttctaa gcaaaagatg aaggaagtgg agttgaggct attatcccgt
      541 taccgtgatt tatttaataa tatgccattg ccttatatac ggcagcggct gattcgggaa
      601 ggtacgcaaa tcgatgtgca ggttttggat gtaaagcatg ctttcgagga aaaaatcgct
      661 cctaaagatt tcgtggtaaa taagcatgga aaagagatcg ctaatttaat tgggggaagt
      721 tatcctttgc ttctatcggc tgtacctaca gttctggaga gtgggaaatc ctttacttgt
      781 gagtattatt ttgagccgac agggctttac tatacgatta tcatcatgcc tacctcggaa
      841 gagaatgtgg ttgatgcgtt ttttattgat attacggata tccataaatt ccagacgcat
      901 ttgaaagcca tgaatcataa gctggctatg gcgttagagg cggcagatct tttaccgtgg
      961 cgatataatc tggctgaagg gaagattatc tatgagtcga aagtacatcc gggagataat
     1021 atagagacag cggaggtgca tacccatgag gtttcgttga aggaatattt cagcaagata
     1081 cacccgtctt tcagggcttg cgtggaaaag gctttcgatg atttgtgtaa tgggaaaata
     1141 aagaaagtac gaaaagaata ttgtctggag cggttgattc cgggagaaga tcgccatgag
     1201 tgggaggaga tacaggtaat ggccgaatat gatgcttccg gtaaaccgaa agcgttgatc
     1261 gggtctacga tatctattac cgagcgtaag caattggaac atgaccttcg aatggctagg
     1321 gataaggcgg aagagtcgaa taaattgaaa tcagcgtttt tagcaaatat gagccatgag
     1381 atacgtacac cgttaaatgc gatcgtaggt ttctctaata tccttgcctc gaccgatgac
     1441 cttgaggata agaaggaatt cgctgatatt atcgagaata ataattccct attgttgcag
     1501 ttgataaacg atatcttgga tttgtcaaag attgaggcgg gtaccttgga gtttacttat
     1561 gactatgtgg atattaacgc tgtattgagg gatttggagc aatcggctca tttgaagaat
     1621 aagaatccgg atgtacagat ctcatttaag gattatctgg agccttgcgt gatttatacg
     1681 gatcgaaatc gtctgtctca actgatgatc aatctgttga acaatgccat gaaatttacg
     1741 caggcaggat cgatcttatt tggctataaa ctgcgggatg ataatacgtt gtggttctat
     1801 gtagaggata cgggctgcgg aatcccggag aataaacgga aagatatttt cggacgtttt
     1861 gtgaaactga atactttcgc tcaaggaaca ggattgggtt taagcatctg tgagatgatt
     1921 gttacccaga tgaaaggaac gataggggtt gattccgtgg aagggaaagg ctctaggttc
     1981 tggtttacat tgccttttca gccgaagaaa gaattattgc ctaatgaaga gcagaagcct
     2041 gttactttgg agaagatcgc ccgaagtgag gttacgatct tgatcgctga ggataattcc
     2101 agtaattatc gcctgtttga gtctatattg aaacctgaat ataaattgat tcatgcatgg
     2161 aatggaaagg aggctgtgga gctatttcat cagcacaaac cgcagttgat cttgatggat
     2221 ataaagatgc cggtcatgga tggatacgag gctaccgcag agattcgtaa ggtttctgct
     2281 agcgtgccta ttattgcggt gacggcttat gctttcgccc aagatgagca acggatcatt
     2341 aatagtggat ttgatgccta tacggcgaaa ccgattaatg ggaagatctt aaaagacaag
     2401 atatcaactt tattgacgca tcggattatt ctgatgtaat cgaagggctt tgcgggaagc
     2461 agaccttata aaacaattaa agcctgtctc acgacaagcc ctaaccaaac tttgttaacc
     2521 ttaaatctaa tactattatg aaaaaaccac gatgcaaata tacggagtta ctgttatttg
     2581 tcaatggttt aagtgctaaa ttgtgttttg taacatgtaa taaaatctaa taggaactta
     2641 tgcctcattg aaatctttcg atagagtcgc atatattctt tatacctccc tctttttgga
     2701 tcatctcgcc tatatgagcg gcatttttct tataaagcgg attggtgacc agatcacaaa
     2761 ccttgcgttc gatctcctgc tcgctcgctt tcgctatctt aaccccttcc ggccctaacc
     2821 ctaattggtg gattcgatag gaccaatagg gttgatcgat aatcaacgga gtgattaatt
     2881 ggggctttcc cgcccttcct acgctatggg tcgtgccgca acctccatga tggataacgc
     2941 cgtcgcagtg cgggaatatc aaattatgcg gaaccggctg cttcattaag aataaatgtt
     3001 tatccgcttg taaggtgatt cctgtctttg cccaaccgga accgatgatg agccgataat
     3061 catgtttctt gcaaatatcg accaaggctt tgctgaaacg gttaccatcc ttggaactac
     3121 aactcccgag ggtaaaaaag ataatagggc tttgagcgct gtcgacgaat gatatcattt
     3181 cctcgtaagc cttctcatca tattgaaaag tgtcattgaa gcaatagcct ccgatcgacc
     3241 attcgaacgt ccagtccgga tctacgtttc ctaggtgctg gctgaaaagc aagtaattat
     3301 atgaacgctc tcccgcgtga tagccaaaat tgcggatcgg agcgagtccg tatttagccc
     3361 ggttcttgtt gatcgtgtct ttgaccatga aattggtcat ccggttcatc aacttccata
     3421 ggatggcagg ggtgatgatc gggttgggtt tgggaaatgg caatacggcc gggggaatct
     3481 tcttacccgg caagaacggg gcgtaggcgg tacgtatcaa gggcttcttg caatactcgg
     3541 cgatacttgc gacagcaaat tccgagttgg tcgatactaa aatatcatgc tcttttagta
     3601 ggggaatcaa ttgatcgaac tgtttatcga tcacgttcct catccattta aggaatggcc
     3661 ggaatttatg tgagttttgc gctgcgctat cgatcatgcc gccgatatcg tcaaactgct
     3721 gcaatacgta tttaaatccg tagggtttaa cggtctcctc gaatatttga gggagattga
     3781 tggtcacctt atggccacgt ctttccaatt cggccgctat ggccaaataa ggaaggacgt
     3841 cgccttgtga gcctctggtt actaatagta ttttcatgct acgtttattt atgggctatg
     3901 agggtcttgt tcaccggata ttcagttgac gcaaacgctc gatgtccaga tggtatttgg
     3961 cttccatcgt ttgtagtttc tgctcattga tggctagggg atgattcaat cgtttcgttt
     4021 cccggtatcc tttatatacg gagcgaatca tcttacgtgg attgaaaatg acggatgagg
     4081 tgatccagca gccgtgcgta caccaacagt tggctttata cccatgtccg atagcggcta
     4141 tttcctgttt catcgcttcg ctattcatca ctttacttat atcgcaacca tactctttta
     4201 tatttccgag cggtttccgt agctcgcagg cacgaaaacg tccgtcgtag tcgattacca
     4261 aggtagtctc tccggccgta caatccatcc cccaaggggt cgggtgatct atgttgctgg
     4321 cacgcacatt atataaggta cgtataaggc cgagatagaa gaagcgtgtg atcggtttcc
     4381 ttaagccggt ggtatccgct accatccgat ccgcataagc agaatagata ggggatatgt
     4441 cgtcttgtat tttgcgtagc gttgactcgg ttaggatttt cacgccatcg tcacgggtca
     4501 tgcctctggc ggcctcaatc gtatgcgtgg aggtgcggaa acgtccgtat acccaagtca
     4561 tgaagtcttc tacctggtct atattgtatt tcgtgataac ggttgctata tttttcagga
     4621 ccttgccgtc gtctccgaaa ttctcctgta tcttacgcag ggtttccaag gccttataga
     4681 agtttcccgg tacgcctctc tgtgtatcgt gggtcgtacc gaaaccatcc aaggaaaggc
     4741 ttacgttaat attacaatcc gggcagttct ggcggaaccg ttttacccat tcgacgatcc
     4801 gggctggttt taagccgttc gtagggatgt tcacatcttt gatatggttg tttttgtaga
     4861 ataactccaa tatttccggt agatcctcac gcagggtagg ctcacctccg gatatccata
     4921 atcggttgaa ttgcccggcg gtttctgata tcttcttgat ttcatcgaaa gtgaggtcgg
     4981 gttgcttctt atccatttct tgggcataga aacacatcgc gcatttggcg ttgcatcggc
     5041 ctgttacgaa aaggaagatc gactcgagtt ttctttccgt gctcattgcc ctgaaagaac
     5101 tggtggttcg ttttttgtcc attttatttt tgttttacca tttgtcttat ctggcgcatg
     5161 attttttcct tgtgtttgat caacacccgg agaaaataac ataagaagcc aaataaaata
     5221 cttattagca tatactatat gatctttaaa ggcaaagatc gacgataaaa tgacgctaaa
     5281 caatctctat ttgcagtatt ttatggtcca tgtttaaaag gtaggtctat aaacaattaa
     5341 agcctgtctc acgacaagcc ctaaccaaac tttgttaacc ttaaatctaa tactattatg
     5401 aaaaaaccac gatgcaaata tacggacttt tcaattcctg tcaagggcta taatgctaaa
     5461 ttatgtttta cgacatataa caaaaacaaa aaggcgcctt tcttttagag aaagacgcct
     5521 tttccatgat atactaatca aatattagtc ttgtaattta gcgcaaatga actcgcggtt
     5581 caaacgagcg atgttgctaa cggagatatt cttcggacat tccatctcgc aagcacgtgt
     5641 gttcgtacag ttaccgaagc caagctcatc cattttagcg accatagctt tggcacggcg
     5701 agcagcctcg actttacctt gaggcaacaa agccagctgg ctaaccttag cggatacgaa
     5761 taacatagcg gaaccattct tacaagcggc agcacaagcg ccacaaccga tacaagcggc
     5821 agcgtccatg gctaagtctg cgtccttctt tgggataggt atcgcattag cgtcgggtac
     5881 accacctgtg ttgacagatg tgaaaccacc cgcttggatg attttgtcgt aagcgtagcg
     5941 gtctaccatc aagtcgcgga taacagggaa acctgcggaa cgccacggtt cgatcgtgat
     6001 cgtctctcca tcgtggaaac gacgcatata taactggcaa gtcgtagctc cagttgccgg
     6061 tccgtgcggg tgtccgttta catacaatga acacatgcca cagataccct cgcggcagtc
     6121 gtgatcgaat acgatcggtt ccttaccctc gttgaccaac tgctcgttca agatatccaa
     6181 catttccaag aaagagacac tctgattgat cttctcaatt ttgtatgtct cgaactgtcc
     6241 tttctccttc ggacctctct gacgccaaac cttaagtgtt acacttatat ctttatccat
     6301 gattctaatt ttttctgaga ttgatgatta ttacttcttg tagttacgtg tttgaggaac
     6361 aacgaactca tagttcaaat cctctttcaa catgacagga tctttgtcct cgccttggta
     6421 ctcccagcaa gatacgtaca tgaacttatc gtcatgacgc aaggcctcac cttcttctgt
     6481 ctggtgctcc gtacggaagt gaccaccaca agactcagcg cggtccaaag catcgtgtgc
     6541 catcaatgta ccgatctcga taaagtcggc tacacgtaat gctttctcta actcggtatt
     6601 caagttgtct gcgttgcctg ggatgaatac gttgctccag aactctttct tcaagtcttt
     6661 cagcatctgg atagcttctt gcaaaccttt tgcgtcgcgt gccataccga tatgttccca
     6721 catgatatga cctagtttgc ggtggatagt gtctacggtt tctttaccct tgacgttcat
     6781 caatttttgg atacgatcct tgatggcctt ttctgcctcg acgaactccg gcagatccgt
     6841 gctgaagcga ggaacttgga tttggtcgga caaatagttc tggatcgtgt atggtaatac
     6901 gaaataacca tcggccaatc cctgcatcaa agcggaagca cctaaacggt tcgctccgtg
     6961 atcggagaag tttgcctcac cgatagcgaa cagacccgga atggaggtca tcaactcata
     7021 gtcaacccac aatccaccca ttgagtagtg caatgccggg tagatcatca tcggagtctc
     7081 gtatggatta tcgttcgtga tctcttcgta catatcgaac aagttaccgt atttctgctt
     7141 aacgacctct ttacccaagc gattgatcgc gtcggagaaa tccaagaata cggccaagcc
     7201 cgtgctgttc acgccgaaac ctgcgtcgca acgctcttta gcggcacgag aagccacgtc
     7261 gcgaggaacc aagtttccga atgccggata acgacgctcc aaataataat cacggtctgc
     7321 ctcgggaata tctttacctt tcttcgttcc ggcctgtaga gctttagcgt cttccagttt
     7381 cttcggaacc cagatacggc cgtcgttacg caaggattcg gacatcaaag tcaatttaga
     7441 ttggaagtca cctttcaccg gtacgcaagt cgggtggatc tgaaccatac aaggattagc
     7501 gaagtaagcg cctttcttgt agcattgcca tgcggcggaa c
//
