LOCUS       c00071_gnlPro..         5739 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|FHKDPNIH_71.
ACCESSION   c00071_gnlPro..
VERSION     c00071_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 11:30:15
            Original ID  :: gnl|Prokka|FHKDPNIH_71
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5739
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5739
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1569:4545]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1570..4545
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5739
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5739
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..1399
                     /locus_tag="ctg71_1"
                     /transl_table=11
                     /translation="VNSQKFESYNDPINDYVSDRLLTVSEYMDVIYPEDRSVFYDAMQS
                     LIAGKNVTINFTCRIQTKYDETWQYCDFMGVPFDQDENGDIIRYTGFRQNIPKIQQLNR
                     ELKERNYKIELSFKTVGMSYWDFDVKSLKFSAFNDPINDFYSEKTITPEDYLNATHPDD
                     VVFVRKYMEHMFRGIDKDFNVKYRSKTKWDDEWQTLLVTGIQVERDKEGHVTRYTGITI
                     NNTKWEKMIQQLKELKEKAELSDRLKSAFLANMSHEIRTPLNAIVGFSELMVTCDDPEE
                     KKEYINIIQSNNELLLRLINDILDLSKIESGIIERRKENFNLAKVCNELFVTIQAKMTN
                     PNVELRLDGPNSECWVLLDRNRLKQVWMNFLTNAVKCTKSGYIKMGYGIERKGLRIYVE
                     DTGIGIPDDLHDKVFTRFEKLNEFSQGTGLGLTISRAIVEAAGGEVGFTSKLGVGSTFW
                     AWLPLRP"
     CDS             1570..3810
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg71_2"
                     /sec_met_domain="Gly_radical (E-value: 1.9e-46, bitscore:
                     145.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 2.9e-282, bitscore:
                     925.8, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENECWDHFKGETWKREINVRDFIQSNYTPYEGDDSFLVASSE
                     KTRRVWNKLTEMFKVEREKGVYDAETKLPQGIDVYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTEEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTAILIEEKKRFLNRLDIQEITEEIIQSREEISEQIKAL
                     KAFEKMCASYGFDVTRPAQNAREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEK
                     DIREGKLTEEEAQELVDQLIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTRTSYRYLHTLYNLGPAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRG
                     EYLEYDEVMAKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFM
                     EYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGA
                     IAALASVAKLPFQHAHDGISYTFAVSPATLGKEREVQINNLVSLLDGYFTPDGGQHLNV
                     NVFDKDLLIDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             3811..4545
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 67.6;
                     E-value: 4.7e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg71_3"
                     /sec_met_domain="Radical_SAM (E-value: 6.4e-34, bitscore:
                     106.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWI
                     RHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPL
                     SKERLDNAKAIFSRYKPENNKA"
     CDS             4744..5322
                     /locus_tag="ctg71_4"
                     /transl_table=11
                     /translation="MKTPQEIAEMISKRFYPLPDADLDSLASIIESSKAAKGELLLDNG
                     QISKYLYYVESGLLRQFYYKNKRDITEHFSCEGNIVICIASTFQREPTRLLIEALEPTV
                     YHRISFSEFSDLLKTSLAINRLYQKILEWALMLSQEKADSWRFESARKRYLRFQQEYPE
                     AAKRASVNHIASYLLMTPESLSRVRAGVL"
     CDS             5430..5738
                     /locus_tag="ctg71_5"
                     /transl_table=11
                     /translation="MKIEKITGREILDSRGNPTVEVDILLESGVMGRASVPSGASTGEN
                     EALELRDGDKKRYGGKGVLKAVENINTIIAPALKGMSALDQIGIDHAMLALDGTKTKS"
ORIGIN
        1 tgtaaattcc cagaaattcg aatcttataa tgatccgatc aacgattacg tttcagatcg
       61 attattgacc gtttcggagt atatggatgt tatatacccc gaggaccggt ctgtttttta
      121 cgacgcaatg caatccctga tagctggaaa gaatgtcacc atcaatttta cctgtcgcat
      181 acaaaccaag tatgatgaga cttggcagta ttgtgatttt atgggtgtcc ctttcgatca
      241 agatgaaaat ggagatataa tacgctatac gggattcagg caaaacattc ctaagatcca
      301 gcaattgaat cgggaactta aagaaagaaa ttacaagata gagctttctt tcaagacggt
      361 tggtatgtcg tattgggatt ttgatgtaaa aagcctgaaa ttcagtgctt ttaatgatcc
      421 tattaatgat ttttattcgg aaaagacaat cactcccgag gattatttga acgctacaca
      481 tccggatgat gtggtttttg tgcggaaata tatggagcat atgttccggg gcatagataa
      541 agatttcaac gttaaatatc gttccaaaac caaatgggat gacgagtggc agaccttatt
      601 ggttactgga atccaggttg aaagagataa agaaggccat gtgactcgct ataccggtat
      661 tacgatcaat aatacgaagt gggagaagat gattcagcaa ttgaaggaat taaaggagaa
      721 agcggaactt tcggatcgtc tcaagtctgc gtttctcgct aatatgagtc acgagatacg
      781 taccccgtta aacgctattg ttggtttttc agagttaatg gtaacttgtg atgatccgga
      841 agaaaaaaaa gaatatataa atatcatcca atccaataat gagttgttat tgcgattgat
      901 caacgatatc cttgatcttt ccaagataga gtcaggcata attgaacgta ggaaggagaa
      961 tttcaatctg gctaaggttt gtaatgagtt atttgttacg attcaggcga agatgacaaa
     1021 tccgaatgtc gagttacgtt tagatggtcc caactcggag tgctgggtac ttctggatag
     1081 aaaccggctt aagcaagtat ggatgaattt cctcactaat gcggtaaaat gcaccaaatc
     1141 cggatatatt aaaatgggat atggcattga aaggaaaggt cttcgtatct atgttgagga
     1201 tacgggaatc ggtataccgg atgatttgca tgacaaagta tttacccgtt ttgagaaatt
     1261 gaatgagttc tcgcagggta ccggacttgg cttgacgatc tccagagcta tcgtagaagc
     1321 ggcaggggga gaagtgggct ttacttcaaa acttggtgtc ggatctactt tttgggcatg
     1381 gttgcctctt cgcccttagg tctgactccc catttattgg taatgtcctt ttaaaatagt
     1441 gaaggatgag aagctttttt atcgcaattt atagggattt tagaactggt gtagcatatg
     1501 tgacagaaat atgacgataa ttctcctacc tttgcggcgg taaaaatgtg gtattagata
     1561 agattgttta tgaaatttga gaatgagtgc tgggatcact ttaaaggtga gacctggaaa
     1621 agagagatca atgtaagaga ttttatccag agtaactata cgccttatga aggggatgat
     1681 tcctttttag tcgcttcttc cgagaagacc cgtagggtct ggaacaagct gaccgagatg
     1741 tttaaggtcg agagggaaaa gggagtttat gacgccgaga caaaattacc tcaagggatc
     1801 gatgtatatg gcccgggcta tattgataag gagaatgagg tgatcgttgg gttacaaacc
     1861 gatgctccgc tgaaacgtgg tatcttcccg aagggcggta tccgtatggt tgagaatagc
     1921 ttggaagctt atggttatca tttggacccg atgacgaaag agatcttcac gaagtaccgt
     1981 aaaactcata acgaaggcgt tttctccgca tatacggaag agatgattgc cgcacgtcgt
     2041 tcggctatta tcaccggtct gcccgacgcg tatggtagag gacgtattat tggtgactat
     2101 cgtcgtgtcg ctttatatgg tacggcaatc ttgatcgagg agaaaaagcg tttcttgaat
     2161 cgtttggata tccaagagat cacggaagag atcatccaga gccgtgagga gatttccgaa
     2221 cagatcaagg ctttgaaagc gttcgagaaa atgtgcgcta gctatggctt tgatgtgact
     2281 cgtccggcac aaaacgctcg tgaggcagtt cagtttgtgt acttagctta tctggcggcg
     2341 gtaaaagacc aagatggcgc ggcgatgtct atcggacgta cttctacgtt tttggatatt
     2401 tatatcgaga aagacatccg tgagggtaag ttgaccgaag aggaggcgca agaattggta
     2461 gaccagctga tcatcaagtt gcgtatcgtt cgtttcttac gtacgccgga atacaatgat
     2521 ttgttctccg gtgatccggt ttgggtaaca gagtccttgg gcggccaagg agtggatggc
     2581 cgttctttgg taacaagaac ttcctatcgt tatctccata cattatataa tctaggtccg
     2641 gcaccagaac ccaatttgac ggttctgtgg tttaagaacg ctcccgagaa ctggaaacgt
     2701 ttctgtgcca aggtctctat cgatacatcg gctatccaat atgagaacga cgatttgatg
     2761 cgtccggatt atggagatga ttatggtatc gcttgttgcg tttctcctat gaagatcggt
     2821 aagcagatgc agttcttcgg agcccgtgcg aatttggcga aatgtttatt atatgcgatc
     2881 aacggaggac gggatgagcg cagcggcgta caggtagccc cgatgttcga gccggttcgc
     2941 ggcgagtatc tggaatacga tgaggtgatg gctaagtatg agcaaatgat gcgctggttg
     3001 gcgaaagtgt atgtgaacgc cttgaagata atccattata tgcatgataa atacgcatat
     3061 gaggcattcg agatgtcgct tcacgatgga gatgtagagc gtatccgtgc gacaggtatc
     3121 gccggtcttt ctatcgttgc cgattcgttg gctgctatcc gtgatacgaa agtacgtgtg
     3181 atccgtgacg agcgtggctt ggcggttgat ttcgaacgtg agggcgaata tgttcctttc
     3241 ggtaataacg acgatcgtac ggatagtatc gctgtagaga tcaccgagaa gttcatggaa
     3301 tatttgcgtc agcacgagac ctatcgccat gcgaaaccta cgcagtccat ccttacgatc
     3361 acctctaatg tggtatatgg taagaagact ggtacgacac cggatggccg tatcggtgga
     3421 actcctttcg ctccgggcgc gaaccctatg aacggtcgtg atacgaaagg tgctatcgct
     3481 gcgctagctt cggtagcgaa gcttccattc cagcatgcgc acgacggtat ctcttatact
     3541 ttcgctgttt ctccggctac gttgggaaag gagcgtgaag tgcagatcaa taatctggtt
     3601 agcttattgg atggctattt tactccggat ggaggtcagc atctgaatgt taacgtattt
     3661 gataaggatt tgttgatcga cgctatggaa catcctgaga aatatccgca acttacgatc
     3721 cgtgtatccg gatatgcggt aaacttcgtg aaactgactc gtgaacaaca gttggatgtt
     3781 atttcaagaa ccattaacca ttcactgtaa gtgataaaag gtaagataca ttcattagag
     3841 agtttcggga ccgtagatgg tcccggaatt cgctttgtcg tttttatgca gggctgcccg
     3901 ttgcgttgcc tgtattgcca taacccggat acttggaacc cgaaagggaa ggtgaaatat
     3961 cagatgactc ccggcgagct attgacggaa gtcttacggt ataagagttt tatcgcccgt
     4021 gggggggtga ctgttaccgg tggggaacct ttattgcaac ctgagtttct gaaagagttt
     4081 ttccgtttgt gccaagagca aggtcttcac acagctttgg acacatctgg tttcgtatgt
     4141 acctcaaaag cttgggaagt cttggattat gcggacttag tgttattgga catcaaaacc
     4201 ttaaacccgg atttacatcc gttactggcg ggtgtcaagc aggataatac gcttctgttc
     4261 ttggatgaat tggagagacg tggcatagat acttggattc gccatgttat tgtccccgga
     4321 tatacggata atgacgagtg gctggaggcg ttagctcggt atgtcagttc atataaagtg
     4381 gttcgcaaag tggaattatt gccttatcat acgatgggaa cctataaata tgagcaatta
     4441 ggtctcgatt atccgttaaa gggggtggaa cccttatcaa aagaacgttt agataatgcg
     4501 aaagcgatat tctctcgcta caaaccagaa aataacaagg cctgatctct cgatcaagcc
     4561 ttgcgattat gcaacagcta atcagggaaa acaatctaat ccgattagct tttaaacgat
     4621 agtaataatt ttttgcctag actaaccttg actatttaat atgtgtttgt tttgttatat
     4681 gcaacaaata tctatttttg taaggagata aaaaatgata tagatcagaa aatgatgaat
     4741 agtatgaaga caccacaaga aatagccgaa atgatatcca aacgttttta tccattaccc
     4801 gatgcggatt tggattctct ggcctcgatc atagaatcaa gtaaggctgc gaaaggagaa
     4861 ttattgttgg ataacggaca aatatctaaa tatttatatt atgtggaatc gggattatta
     4921 cgccagtttt actataagaa taaacgtgat attaccgagc atttctcctg tgagggaaat
     4981 atcgttattt gtatagccag cacattccaa agagaaccga cgcgtttatt aatcgaggca
     5041 ttggaaccga cggtttatca ccggatctct ttttcggaat ttagcgattt attgaaaaca
     5101 tctttagcta ttaatagatt gtaccagaaa atactggagt gggctttgat gctttcgcaa
     5161 gagaaagcgg actcttggcg ttttgagtcg gcaagaaaac gttatctgcg ttttcaacag
     5221 gaatatccgg aagcagcgaa acgtgcttct gtcaatcata tcgcctcgta tttattgatg
     5281 acacccgaat ctttgagccg ggtgcgggcg ggagtgttgt agtctctacc tacattcagg
     5341 tattattgag gtgaaaatag caatttgtgg tgatttcacg gttcaagtaa tccatctata
     5401 tttgcgtcag tttaaaaaac gtagaagcaa tgaaaataga aaagattaca ggacgtgaga
     5461 tcctagattc aagaggtaac ccgacagtag aggttgatat tttactagag tccggcgtta
     5521 tgggccgtgc gtctgtgcct tccggtgcgt ccaccggcga gaacgaggct ttggagttgc
     5581 gtgatggcga taagaagcgt tatggtggta aaggcgtatt gaaagcggtt gagaatatca
     5641 atacgattat cgctccggca ttgaagggga tgtctgcctt ggatcagata ggtatcgatc
     5701 atgcgatgtt ggctttggac ggtacgaaaa ctaaatcga
//
