LOCUS       c00358_gnlPro..         2804 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|MNGAOHEH_358.
ACCESSION   c00358_gnlPro..
VERSION     c00358_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 07:03:17
            Original ID  :: gnl|Prokka|MNGAOHEH_358
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2804
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2804
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:2087]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2..2087
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..2804
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2804
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..1360
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg358_1"
                     /sec_met_domain="Gly_radical (E-value: 7.5e-48, bitscore:
                     149.3, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 1.7e-195, bitscore:
                     638.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="ELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTEAIGGRFNDGRV
                     KVTKTSFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKDFCAKVSVDTSSIQYENDNLMR
                     EVRNCDDYGIACCVSYQAIGKQIQFFGARANLAKALLLAINGGRCENTGTVMVKGIPVL
                     TGETLKFEEVMANYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMAFIDTNPRINLAY
                     GVAGLSIAIDSLSAIKYAKVTARRNEIGLTEGFDTEGSFPCFGNNDDRVDHLGVDLVYY
                     FSEELKKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKN
                     GAIASLSSVAKLRYRDSQDGISNTFSIVPKSLGPTPEERVENLVTMMDGYFTKGAHHLN
                     VNVLNREMLEDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             1362..2087
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 75.5;
                     E-value: 1.7e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg358_2"
                     /sec_met_domain="Radical_SAM (E-value: 5.5e-32, bitscore:
                     99.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCPFRCLYCANPDTIDVKGG
                     TPTTPEEILQMAISQKAFFGKKGGITFSGGEPTLQAEALIPLFKELKANGIHICLDSNG
                     GIWNEKVEELFSLTDLVLLDIKQFNPERHRTLTGRSNEQTLRTAAWLEEHEHPFWLRYV
                     LVPGYSDSEEDIRLLGESLGKYRQIQRVEILPYHRLGVHKYEVMGWDYKLKEVKENTPE
                     QLERAEKLFKEYFTNVITN"
     CDS             complement(2156..2803)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fn3-like"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3_C"
                     /gene_functions="other (smcogs) SMCOG1041:Fibronectin type
                     III-like domain (Score: 89.8; E-value: 3.1e-28)"
                     /gene_kind="other"
                     /locus_tag="ctg358_3"
                     /sec_met_domain="Fn3-like (E-value: 1.5e-29, bitscore:
                     89.8, seeds: 729, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3_C (E-value: 4.8e-26,
                     bitscore: 79.6, seeds: 116, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VMCGRPVIFNEIREHADAVLCTWWLGSEAGNAICNVLWGEYNPSA
                     KLPMTFPAHVGQIPIYYQNKSTGRPTALQQTYKASYIDASTEPAYPFGYGLSYTDFGYS
                     DLKLLPGKDAGVHTEVSVKVTNTGKCAGEEVVQLYVQDKVASVTRPIKELKGFRKIHLN
                     AGESKTVAFQITDDALGFYDNEMNYMVEPGDFVFMVGGSSDDVQQITYNLKE"
ORIGIN
        1 agaactgata gaccagttcg tcatcaagct gcgcatggta cgccatctgc gtatgcagtc
       61 gtacaatgat atctttgccg gtgacccgac ttgggtgacc gaagccatcg gaggacgctt
      121 caatgacgga cgcgtgaaag tcaccaagac ttctttccgc ttcttgcaga cgctatacaa
      181 cctcggtcct tctccggaac caaacttgac cgtactgtgg agtcccgaac tgccggaagg
      241 cttcaaggat ttctgcgcca aagtttccgt agataccagc tccatccagt acgaaaacga
      301 caacctgatg cgcgaagtgc gcaactgcga cgattacggc attgcctgct gtgtatccta
      361 ccaagccatc ggcaagcaaa tccagttctt cggcgcacgt gccaaccttg ccaaggccct
      421 gctgcttgcc atcaacggtg gccgttgcga aaacaccgga actgtcatgg tgaaaggtat
      481 tcccgtattg accggcgaga cactgaagtt tgaggaagtt atggccaact acaagaaggt
      541 actgactgaa atagcccgtg tctacaacga ggccatgaat atcatccact acatgcatga
      601 taagtattat tatgagaagg cccagatggc attcatcgat accaacccac gcatcaatct
      661 ggcatacggt gttgccggcc tttccatcgc catagactca ctgtccgcca tcaaatatgc
      721 caaagtcact gcccgccgca acgaaatcgg cctgaccgaa ggtttcgaca ctgaaggctc
      781 attcccctgc ttcggcaata atgacgaccg tgtggaccac ctgggagtag acctcgttta
      841 ctatttcagc gaagaactga aaaagctgcc tgtctacaag aatgcccgtc ccaccctttc
      901 cttgctgact atcacctcca acgtgatgta cggcaagaag accggtgcca ctccggacgg
      961 acgcgccaaa ggtgttgcct ttgctccggg tgccaaccca atgcacggac gtgacaagaa
     1021 cggtgccatc gcttccttga gctccgtagc caaattgcgc taccgcgact cgcaggacgg
     1081 catcagcaat acgttctcca ttgttcctaa atcactgggc ccgacaccgg aagaacgtgt
     1141 agagaacctg gtaaccatga tggacggcta cttcaccaaa ggtgcccacc atttgaacgt
     1201 aaacgtactg aaccgcgaaa tgctggaaga tgccatggaa catcctgaaa agtatccgca
     1261 actcaccatc cgcgtttccg gctatgccgt gaacttcgtg aagttgagcc gcgagcatca
     1321 gttggaagtc atcagccgtt cattccacga aagaatgtaa tatgataaat gtacactcct
     1381 acgaaagtat gggaacattc gacgggccgg gactccggct cgtcgttttc ctgcaaggat
     1441 gtccgttccg ttgtctgtat tgtgccaatc cggatacgat tgatgtaaaa ggaggcactc
     1501 ccaccactcc ggaagagatt ctgcaaatgg ctatcagcca aaaggcattc ttcgggaaga
     1561 aaggaggtat tactttctcc ggcggtgagc ctaccctgca agcagaagcg ctgattcctc
     1621 ttttcaagga gctgaaggcg aacggcatcc atatctgtct ggacagtaac ggaggcatct
     1681 ggaatgaaaa ggtggaagag ctctttagcc tgactgacct cgtgctgctg gacatcaagc
     1741 aattcaatcc cgaacgccac cgtacgctga cgggacgaag caacgaacag acactccgca
     1801 ccgctgcctg gctcgaagaa catgaacacc ccttctggct gcgttatgta ctggttcccg
     1861 gttacagcga ttcggaagaa gacatccgcc tgctgggtga aagtctcggc aaataccggc
     1921 agatacaacg ggtggaaata cttccttacc accgtctggg cgttcacaaa tacgaagtca
     1981 tgggatggga ttacaaattg aaagaagtga aggaaaatac tccggaacaa ctggaacggg
     2041 cagaaaaact gttcaaagag tatttcacga atgtgataac caactaaaac agaaaagcaa
     2101 ggctgtccgg atatgattcc agacagcctt gcttttcatt tataggacaa gaatcctatt
     2161 ctttcaagtt ataggtaatc tgctgtacat catcactact gccacccacc ataaagacga
     2221 aatcaccagg ttctaccata taattcatct cattgtcata gaatcccagt gcatcgtcag
     2281 ttatctgaaa agctacagtt ttgctttcac ccgcattcag atgaattttc ctgaagccct
     2341 tcaactcctt tatcgggcgc gtaacagacg ctaccttatc ctgcacatac aactggacta
     2401 cttcctctcc tgcacactta ccggtattag tcacttttac cgacacctcc gtatggacgc
     2461 ctgcatcctt gcccggcagc agtttcaaat cagaatatcc aaaatccgta tacgaaagcc
     2521 catatccgaa aggataggca ggctcagtgg aagcatcgat ataggatgcc ttataagtct
     2581 gttgcaatgc ggtaggacga ccggtactct tattctggta ataaatggga atctgcccta
     2641 cgtgtgcagg aaatgtcatg ggcaatttag ccgaaggatt atactctccc cacaacacat
     2701 tgcatatggc attgccggcc tcactcccca gccaccaagt acacagtacc gcatcagcat
     2761 gttcccttat ctcattgaag attaccggac gcccacacat gaca
//
