LOCUS       c00469_gnlPro..         3181 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KNHNLHNI_469.
ACCESSION   c00469_gnlPro..
VERSION     c00469_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 06:41:50
            Original ID  :: gnl|Prokka|KNHNLHNI_469
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3181
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3181
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[67:3043]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      68..3043
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3181
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3181
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             68..2308
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg469_1"
                     /sec_met_domain="Gly_radical (E-value: 7.8e-47, bitscore:
                     145.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 8.7e-282, bitscore:
                     922.9, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENECWDHFKGETWKREINVRDFIQSNYTPYEGDDSFLVASSE
                     KTRRVWNKLTEMFKVEREKGVYDAETKLPQGIDVYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTEEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTAILIEEKKRFLNRLDIQEITEEIIQSREEISEQIKAL
                     KAFEKMCASYGFDVTRPAQNAREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEK
                     DIREGKLTEEEAQELVDQLIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTRTSYRSLHTLYNLGPAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRG
                     EYLEYDEVMAKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFM
                     EYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGA
                     IAALASVAKLPFQHAHDGISYTFAVSPATLGKEREVQINNLVSLLDGYFTPDGGQHLNV
                     NVFDKDLLIDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             2309..3043
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 67.6;
                     E-value: 4.7e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg469_2"
                     /sec_met_domain="Radical_SAM (E-value: 2.6e-34, bitscore:
                     106.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWI
                     RHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPL
                     SKERLDNAKAIFSRYKPENNKA"
ORIGIN
        1 acagaaatat gacgataatt ctcctacctt tgcggcggta aaaatgtggt attagataag
       61 attgtttatg aaatttgaga atgagtgctg ggatcacttt aaaggtgaga cctggaaaag
      121 agagatcaat gtaagagatt ttatccagag taactatacg ccttatgaag gggatgattc
      181 ctttttagtc gcttcttccg agaagacccg tagggtctgg aacaagctga ccgagatgtt
      241 taaggtcgag agggaaaagg gagtttatga cgccgagaca aaattacctc aagggatcga
      301 tgtatatggc ccgggctata ttgataagga gaatgaggtg atcgttgggt tacaaaccga
      361 tgctccgctg aaacgtggta tcttcccgaa gggcggtatc cgtatggttg agaatagctt
      421 ggaagcttat ggttatcatt tggacccgat gacgaaagag atcttcacga agtaccgtaa
      481 aactcataac gaaggcgttt tctccgcata tacggaagag atgattgccg cacgtcgttc
      541 ggctattatc accggtctgc ccgacgcgta tggtagagga cgtattattg gtgactatcg
      601 tcgtgtcgct ttatatggta cggcaatctt gatcgaggag aaaaagcgtt tcttgaatcg
      661 tttggatatc caagagatca cggaagagat catccagagc cgtgaggaga tttccgaaca
      721 gatcaaggct ttgaaagcgt tcgagaaaat gtgcgctagc tatggctttg atgtgactcg
      781 tccggcacaa aacgctcgtg aggcagttca gtttgtgtac ttagcttatc tggcggcggt
      841 aaaagaccaa gatggcgcgg cgatgtctat cggacgtact tctacgtttt tggatattta
      901 tatcgagaaa gacatccgtg agggtaagtt gaccgaagag gaggcgcaag aattggtaga
      961 ccagctgatc atcaagttgc gtatcgttcg tttcttacgt acgccggaat acaatgattt
     1021 gttctccggt gatccggttt gggtaacaga gtccttgggc ggccaaggag tggatggccg
     1081 ttctttggta acaagaactt cctatcgttc tctccatacc ttatataatc taggtccggc
     1141 accagaaccc aatttgacgg ttctgtggtt taagaacgct cccgagaact ggaaacgttt
     1201 ctgtgccaag gtctctatcg atacatcggc tatccaatat gagaacgacg atttgatgcg
     1261 tccggattat ggagatgatt atggtatcgc ttgttgcgtt tctcctatga agatcggtaa
     1321 gcagatgcag ttcttcggag cccgtgcgaa tttggcgaaa tgtttattat atgcgatcaa
     1381 cggaggacgg gatgagcgca gcggcgtaca ggtagccccg atgttcgagc cggttcgcgg
     1441 cgagtatctg gaatacgatg aggtgatggc taagtatgag caaatgatgc gctggttggc
     1501 gaaagtgtat gtgaacgcct tgaagataat ccattatatg catgataaat acgcatatga
     1561 ggcattcgag atgtcgcttc acgatggaga tgtagagcgt atccgtgcga caggtatcgc
     1621 cggtctttct atcgttgccg attcgttggc tgctatccgt gatacgaaag tacgtgtgat
     1681 ccgtgacgag cgtggcttgg cggttgattt cgaacgtgag ggcgaatatg ttcctttcgg
     1741 taataacgac gatcgtacgg atagtatcgc tgtagagatc accgagaagt tcatggaata
     1801 tttgcgtcag cacgagacct atcgccatgc gaaacctacg cagtccatcc ttacgatcac
     1861 ctctaatgtg gtatatggta agaagactgg tacgacaccg gatggccgta tcggtggaac
     1921 tcctttcgct ccgggcgcga accctatgaa cggtcgtgat acgaaaggtg ctatcgctgc
     1981 gctagcttcg gtagcgaagc ttccattcca gcatgcgcac gacggtatct cttatacttt
     2041 cgctgtttct ccggctacgt tgggaaagga gcgtgaagtg cagatcaata atctggttag
     2101 cttattggat ggctatttta ctccggatgg aggtcagcat ctgaatgtta acgtatttga
     2161 taaggatttg ttgatcgacg ctatggaaca tcctgagaaa tatccgcaac ttacgatccg
     2221 tgtatccgga tatgcggtaa acttcgtgaa actgactcgt gaacaacagt tggatgttat
     2281 ttcaagaacc attaaccatt cactgtaagt gataaaaggt aagatacatt cattagagag
     2341 tttcgggacc gtagatggtc ccggaattcg ctttgtcgtt tttatgcagg gctgcccgtt
     2401 gcgttgcctg tattgccata acccggatac ttggaacccg aaagggaagg tgaaatatca
     2461 gatgactccc ggcgagctat tgacggaagt cttacggtat aagagtttta tcgcccgtgg
     2521 gggggtgact gttaccggtg gggaaccttt attgcaacct gagtttctga aagagttttt
     2581 ccgtttgtgc caagagcaag gtcttcacac agctttggac acatctggtt tcgtatgtac
     2641 ctcaaaagct tgggaagtct tggattatgc ggacttagtg ttattggaca tcaaaacctt
     2701 aaacccggat ttacatccgt tactggcggg tgtcaagcag gataatacgc ttctgttctt
     2761 ggatgaattg gagagacgtg gcatagatac ttggattcgc catgttattg tccccggata
     2821 tacggataat gacgagtggc tggaggcgtt agctcggtat gtcagttcat ataaagtggt
     2881 tcgcaaagtg gaattattgc cttatcatac gatgggaacc tataaatatg agcaattagg
     2941 tctcgattat ccgttaaagg gggtggaacc cttatcaaaa gaacgtttag ataatgcgaa
     3001 agcgatattc tctcgctaca aaccagaaaa taacaaggcc tgatctctcg atcaagcctt
     3061 gcgattatgc aacagctaat cagggaaaac aatctaatcc gattagcttt taaacgatag
     3121 taataatttt ttgcctagac taaccttgac tatttaatat gtgtttgttt tgttatatgc
     3181 a
//
