LOCUS       c00224_gnlPro..         6761 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KGFAHJCF_224.
ACCESSION   c00224_gnlPro..
VERSION     c00224_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 08:23:03
            Original ID  :: gnl|Prokka|KGFAHJCF_224
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6761
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6761
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:2321]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..2321
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..6761
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6761
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             3..1538
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 121.8; E-value:
                     1.3e-37)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg224_1"
                     /sec_met_domain="FAD_binding_2 (E-value: 1.3e-38, bitscore:
                     121.8, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 3e-39, bitscore:
                     122.7, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="REYGGLLDNRSFGGAQVSRTFYARGQTGQQLLLGAYSALSRMIGL
                     NKVKMYTRHEMLDVVKVDGRARGIIARNLITGQIERFSAHAVVVATGGYVNTFFLSTNA
                     MASNGSAAWQCYKKGAYFANPCMVQIHPTCVPVKGDYQSKLTLMSESLRNDGRIWVPKK
                     LEDAKALQAGTKKGKDIPEADRDYYLERRYPAFGNLVPRDVASRAAKERCDAGFGVNNT
                     GLAVFLDFSDAINRLGKDVVKAKYGNLFDMYEEITNDDPYETPMMIYPALHYSMGGLWV
                     DYELMTSIPGLFAIGEANFSDHGANRLGASALMQGLADGYFVLPYTIQNYLSDQIQVPR
                     FSTDLPEFAEAEKGIKDRIKKLMSIKGKESVDSIHKKLGHIMWEHVGMARDAKGLQEAI
                     QMLKELKKEFWSNVYIPGEADNLNVELDKALRLADFIEIGTLMAHDALDRAESCGGHFR
                     TEHQTEEGEALRHDDKFMYVSCWEYQGEDKDPVMLKEDLNYQFVVPQTRNYKK"
     CDS             1566..2321
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg224_2"
                     /sec_met_domain="Fer2_3 (E-value: 4.1e-27, bitscore: 83.2,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 1.3e-12, bitscore: 36.8,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 1.4e-06, bitscore: 17.5,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKDIKVTLKVWRQKSPKDKGQFETYQIDKINQSVSFLEMLDILN
                     ERLVSEGKEPIVFDHDCREGICGMCSLYVNGHPHGPATGATTCQLYMRRFNDGETITIE
                     PWRSAGFPVIRDLMVDRYAYDKIIQAGGFVSVNTGGVPDANSIAIPRADAEMAMDAAAC
                     IGCGACAAACKNGSAMLFVSAKVSQLALLPQGKVEAARRAKAMVAKMDELGFGNCTNTR
                     ACEMECPKNISVSNIARLNREFICAKLQD"
     CDS             complement(2620..3465)
                     /locus_tag="ctg224_3"
                     /transl_table=11
                     /translation="MEDNIPKFDLPVDFIVGNSITGEILNQYGRFPCKIKAGIFVLCVQ
                     GTVHATVNLTEFTIRKNDFITLPPGCFIQIHEVSDDVKLCFAGFSSVFVSHINYIKTIT
                     NYLPVIFDSPIMPLPEHISLLYQQAFDLLINAYSMPKTIENKEIIKAIFTIFMQGVIEL
                     YRNHTPYSNAPMTRNMEICREFVQLAMENYTKEHSVSYYAKRLNITLQHFCYVIKKVSG
                     RTALDILSNIIIMDAKAQLKSTDMPVKKIAFALGFDNLSFFNKYFRQHVGMTPQEYRES
                     "
     CDS             3706..4920
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Radical_SAM"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg224_4"
                     /sec_met_domain="Radical_SAM (E-value: 8.4e-15, bitscore:
                     44.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTKRTKSSFRTMSTNRKLESVFLFVTGRCNAKCKMCFYAEDMDK
                     KEKDLTFEEIKTISETAGQFNRLWVSGGEPTLREELPEIIEMFYKNNEIKDVNIPTNGL
                     KPDRIVAWVKQIRQNCPELNINLSLSLDGFGDTHDIQRGVPGNFYKAMGTLKLIEKNFA
                     DDGRVLKNLATVITKYNENEVEDLMIWMLGRFHLSNHIIEAARGMTRDDGVKVLTEPTL
                     RALQDKVVPIYNVYGERMAEETGGFRKPIARFFYLGFIRTLYNVRATNIDHPTPWGMDC
                     TAGETTLVIDYDGRFRACELREPLGNVKDYDCNIAKIMESDAMKQEIAAIGHGDKANCW
                     CTHGCWITSSLVFNPRKMITQVYKGYREMKKLNRPLNISEELLQSIERKYALDEQKLVE
                     LGVRN"
     CDS             5098..5682
                     /locus_tag="ctg224_5"
                     /transl_table=11
                     /translation="MTTKLKDLFDTFLNIAASSQKLGINLIRVAILIIFVWIGGLKFYN
                     YEAEGIVPFVANSPFMSFFYAKSAPEYKEYKLKEGEFNEAKHQWHVENNTYIFSHGLGI
                     MIMAIGILTFLGIFSPKIGLIGSGLAIIMTLGTLSFLVTTPEVWVPDLGSGEHGFPLLT
                     GAGRLVIKDTAIIAGAIVVLSDCAQRILKKK"
     CDS             6088..6759
                     /locus_tag="ctg224_6"
                     /transl_table=11
                     /translation="MNLRKNLFWAIPFIVVGVVWIMRNTGMITDDLFHIIVSWQMLVMY
                     IGIGSISRRHYIGGLITFLVGAAFLLPRLGWMDQLPGKLCHHIQDQKACRSWKRIAQCA
                     EKRPWKQRRSGSQHGQKIHQRNQRCQQHGIINLHEEKTRCQNESYDSHDLKLCAKISPY
                     GIFQVSPDHPKPFPCLFRYDMPQDSVNPLPLRNKKISKQKREDKGNQKRRNIFCCISRR
                     FY"
ORIGIN
        1 ctcgcgaata cggtggtttg ctggataacc gttcattcgg tggtgctcag gtatcccgta
       61 cgttctatgc ccgcggtcag accggacagc agttgttgct gggtgcttac tctgctttga
      121 gccgtatgat cggtttgaac aaagtaaaga tgtatacccg ccacgaaatg ctggacgttg
      181 tgaaggtgga tggccgtgcc cgcggtatca tcgcacgtaa cctgatcaca ggtcagatcg
      241 aacgtttttc tgctcacgca gtagtggtgg caacaggtgg ttatgtaaat actttcttcc
      301 tgtctactaa cgcaatggct tccaacggtt ctgctgcatg gcagtgctac aagaaaggtg
      361 cttacttcgc taacccttgt atggtgcaga tccacccgac ttgtgtaccg gtgaaaggtg
      421 actaccagtc taagctgact ttgatgtctg aatcattgcg taacgacggt cgtatctggg
      481 ttccgaagaa actggaagat gcaaaagcat tacaggccgg aactaagaaa gggaaagaca
      541 ttcctgaagc agaccgcgac tactatctgg aacgccgtta tccggcattc ggtaacctgg
      601 ttccgcgtga cgtggcttca cgtgccgcta aagaacgttg cgacgccggc ttcggtgtga
      661 acaacacagg tctggccgtg ttcctcgact tctccgacgc catcaaccgt ctgggtaaag
      721 atgttgtaaa agctaaatat ggtaacttgt tcgacatgta cgaagaaatt accaacgacg
      781 atccatacga aactccgatg atgatttatc ctgcattgca ctactcgatg ggtggtctgt
      841 gggttgacta tgaattgatg acttctattc cgggtctgtt cgctatcggt gaagctaact
      901 tctctgacca cggagctaac cgtctgggtg cttctgcgtt gatgcaggga ttggccgatg
      961 gctactttgt attgccttat acaattcaga actatctgtc agaccagatc caggttccac
     1021 gtttcagcac cgacctgcct gagtttgcag aagctgaaaa ggggatcaaa gaccgcatca
     1081 agaaattgat gagtatcaag ggtaaagagt cggtagattc tatccataag aaactgggtc
     1141 atatcatgtg ggaacatgta ggtatggcac gtgatgcaaa aggtttgcag gaagcgatcc
     1201 agatgttgaa agagctgaag aaagagttct ggagtaatgt atatattccg ggtgaagccg
     1261 ataacctgaa cgtagagttg gataaggcat tgcgtttggc tgactttatt gagattggta
     1321 cattgatggc acacgatgca ttggaccgtg ccgaatcttg tggaggacac ttccgtacag
     1381 agcaccagac cgaggaaggc gaagccctgc gtcatgacga taagttcatg tacgtatctt
     1441 gttgggaata ccagggtgaa gacaaagatc cggttatgct gaaggaagat ttgaactatc
     1501 agttcgttgt tcctcaaaca cgtaactaca agaagtaata atcatcaatc acaaaaatta
     1561 gaatcatgga taaagatata aaagtaacac ttaaagtttg gcgtcagaaa agtccgaaag
     1621 ataaaggaca atttgagact taccaaatag acaaaatcaa tcagagtgtt tctttcctgg
     1681 aaatgttaga cattttgaat gaacgtttgg tcagtgaagg taaagagccg atcgtattcg
     1741 atcacgactg tcgtgaaggt atctgcggta tgtgttcttt gtatgtaaac ggacatccgc
     1801 acggacctgc aaccggcgca actacttgcc agctttatat gcgtcgcttc aatgatggcg
     1861 aaactattac aatcgaaccg tggcgttctg ccggtttccc ggtaatccgc gacttgatgg
     1921 tagaccgtta tgcttacgat aaaattatcc aggcaggtgg tttcgtatca gtgaacacag
     1981 gtggtgtacc tgatgccaat tcaatcgcta ttcctcgtgc agatgctgaa atggctatgg
     2041 atgctgctgc atgtatcggt tgcggtgctt gtgctgctgc ttgtaagaac ggttcggcta
     2101 tgttgttcgt ttctgcaaag gttagccagt tggctctgtt gcctcagggt aaggtagaag
     2161 ctgctcgtcg tgcgaaagca atggttgcca agatggacga actgggcttc ggtaactgta
     2221 ccaacacgcg tgcttgcgaa atggaatgtc cgaagaacat ctctgtcagc aacatcgctc
     2281 gtttgaaccg cgagttcatt tgtgctaaat tgcaagacta atatacatta tatatgtatg
     2341 tccggagagg cgttgcttat ggcagcgcct cttctttttt gcccctattt taacatttct
     2401 gaagtcatag ataaatatct tgaatgttgt tagtgtttct tatatgctat acaacactaa
     2461 ttagcatttt aactattgac aagttggaga atacctgtat ctttgtactg tggttttttc
     2521 ataatagtat tagatttaag gttaacaaag ttggttaggg cttgtcgtga gacaggcttt
     2581 aattgtttat aggggtttgc gtccggcata tccctttttt tagctctccc tgtattcctg
     2641 cggggtcatt cctacatgtt gccggaaata tttgttgaag aacgaaaggt tgtcgaaacc
     2701 cagggcgaat gcaattttct tgaccggcat atcggtcgat ttaagctgcg ctttggcatc
     2761 cataatgatg atattgctca ggatatccaa tgcagtccgt ccgctgacct tttttatcac
     2821 ataacagaaa tgctgcaagg tgatgttgag ccttttggcg taataagaca cgctatgctc
     2881 tttcgtgtag ttttccatcg ccagttgtac gaactcccgg catatttcca tgttacgcgt
     2941 catgggagca ttgctatatg gcgtatgatt cctgtataat tcaataaccc cttgcatgaa
     3001 gatagtaaag attgccttga taatctcctt gttctcgatg gttttaggca tggaataagc
     3061 attgatcagc aagtcgaaag cctgttggta gagtaaggat atatgttccg gcagaggcat
     3121 gatcggactg tcgaatatga caggcagata gttcgtaatc gtctttatat aattgatatg
     3181 gctgacgaat acggaagaaa agcctgcaaa acacagtttt acatcatcgg aaacctcatg
     3241 tatctgaatg aagcaacccg ggggtaaggt gataaagtcg ttcttccgga tggtaaactc
     3301 agttaagttt acagtcgcgt ggactgtccc ttgtacgcaa agcacgaata tgccagcctt
     3361 gatcttgcac gggaaacgtc cgtactggtt cagtatttca cctgtaatac tgtttcctac
     3421 aataaaatct acggggaggt cgaatttagg aatattgtct tccatacttt tatatacact
     3481 taattgaatg tacaaagata gtttttttga gacaagttag tcgataatct taaatataga
     3541 acatcgaatc ccacaaatgg agattgtttt gtgctgtata atagttgaaa tttgcgaatg
     3601 ttttcaagtc ttgctgatag atttgttggg atggtatata tttgtctatc tgctaaataa
     3661 gatatgggag ctaatactaa tagagaaatc taatcgataa taacaatgag tacgaaaaga
     3721 acaaagagct ctttcaggac aatgagcaca aacagaaaat tagaatctgt tttcctgttt
     3781 gtcacaggac ggtgtaatgc taaatgtaaa atgtgtttct atgcagaaga catggataaa
     3841 aaggagaaag acctgacttt tgaagaaatc aagacaatct ccgaaacagc cgggcagttc
     3901 aaccgtttgt gggtatccgg cggtgaaccg acactgcgtg aggagttacc tgaaattata
     3961 gagatgttct acaagaataa cgagataaag gacgtgaata tccctaccaa cggattgaag
     4021 cctgaccgga tcgtcgcctg ggtaaaacag atccgccaga attgtccgga actgaatatc
     4081 aacctgagcc tttctttgga cggtttcggt gatacgcatg atatacagcg cggcgttccc
     4141 ggcaatttct acaaagcgat ggggacactg aagctgattg aaaaaaactt tgctgacgac
     4201 ggcagggtgt tgaaaaacct ggccaccgta atcaccaaat ataacgagaa tgaggtcgag
     4261 gatctgatga tatggatgct gggacgtttc catctttcca atcatattat tgaagctgca
     4321 cgcggtatga cccgcgacga cggagtgaag gttcttaccg agcctactct acgcgccttg
     4381 caggataagg ttgttcctat ttataatgta tatggggagc gtatggcgga ggaaaccggt
     4441 gggttcagaa aacctatcgc ccgtttcttc tacctgggtt tcatacgtac attatataat
     4501 gtacgtgcga ccaatatcga tcatccgaca ccctggggta tggattgtac ggccggcgaa
     4561 acaacactgg taatagacta tgacggtcgt ttccgtgcct gtgaacttcg cgaaccgctc
     4621 ggtaatgtca aggattatga ctgcaatatc gcaaagatta tggagagcga tgccatgaaa
     4681 caagagattg ccgctatcgg tcatggtgac aaggctaact gctggtgtac gcatgggtgt
     4741 tggatcactt catcgcttgt tttcaatccc cgcaagatga tcacgcaggt ctataaagga
     4801 taccgggaga tgaagaagct gaaccgtcct ctgaatatca gtgaagagct tttgcagagt
     4861 atcgaacgca aatatgcctt ggacgaacag aaactggtgg agctgggagt tcgtaattaa
     4921 aatagttccg ataacggatc atttataaag ccggttgacg ggaaatatac ctctgttaac
     4981 cggctttatt gtgtttaagg gaataattgt caaattatcc ggtagaattg ccatttccca
     5041 taggggaaaa atgtcggatc tttgcatcat aagttaaact gataaacaaa gaaagacatg
     5101 actactaaat taaaagattt attcgacaca tttctgaaca ttgcagcttc ttcacagaaa
     5161 ctgggtatta acctgatccg cgtagctatc ctgattatat tcgtatggat cggcgggctg
     5221 aaattctata attatgaagc ggaagggatc gttccttttg tggcaaacag tccgtttatg
     5281 agctttttct atgcaaagag tgcaccggaa tacaaagaat ataagttgaa agaaggtgaa
     5341 ttcaatgaag ccaagcatca gtggcatgta gaaaacaata cctatatttt ttcacatgga
     5401 ctaggcatta tgatcatggc gatcggtatc ctgactttcc tgggtatctt ttctccgaaa
     5461 attggcttga taggttccgg actggcgatc ataatgacat tgggtacgct ttcattcctg
     5521 gtgacaacac ctgaagtgtg ggtgcccgat ttgggaagcg gagaacatgg tttcccctta
     5581 ctgaccggag caggtcggct ggtaataaaa gatacggcaa tcatagccgg agcgatcgtg
     5641 gtattgtccg actgtgcaca acgtatatta aagaagaaat aggaataaaa agtgtttatt
     5701 ttaacacttt ggaagcaaaa aacaaacata ttgaaccttg ttagcttttc ttatagtgta
     5761 tgaaacacca tttagcataa aacctcttga caggtttgga ttaagcagta tatttgcatc
     5821 gtggtttttt cataatagta ttagatttaa ggttaacaaa gttggttagg gcttgtcgtg
     5881 agacaggctt taattgttta taggggtttg cattcggaaa tgcaagcctt ttttctttta
     5941 aatatggcat tctctgaaaa ataactccct tttttatcgg taatattcgg caattcaccg
     6001 atatttgact gtaataggac gtatttctat tgccggaagg ctgacaggag catacttttg
     6061 ctctcaaaca attaaactgt atatcgtatg aatcttagga aaaacttatt ttgggccatc
     6121 ccgtttattg tagtgggggt cgtatggatc atgaggaaca caggcatgat cacggatgat
     6181 ctttttcata tcattgtatc ctggcagatg cttgtaatgt atataggtat agggagtatt
     6241 tcccggagac attatatagg cggtttgatt acctttttgg ttggtgcggc atttttactg
     6301 ccacgactgg ggtggatgga tcagctgcct ggtaagctgt gtcaccacat acaggaccag
     6361 aaggcctgcc gctcttggaa gcgcatcgct caatgcgcag aaaagcgccc atggaagcag
     6421 cgccgttccg gttcccagca tgggcagaaa atccatcagc gcaatcagcg ctgccagcag
     6481 cacggcataa tcaatctgca tgaagagaaa acccgctgcc agaatgagtc ctacgactcc
     6541 catgatctta aactgtgcgc gaaaatatcc ccctacggca tatttcaggt ttccccagac
     6601 catccgaagc ccttcccttg tctgttccgg tatgacatgc cgcaggattc ggtgaacccg
     6661 ctcccgctgc gcaataaaaa aataagcaaa cagaagcgag aagataaagg aaatcagaag
     6721 cgacggaata ttttttgctg catttcccgc cgcttctacc g
//
