LOCUS       c00331_gnlPro..         5221 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KGFAHJCF_331.
ACCESSION   c00331_gnlPro..
VERSION     c00331_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 08:23:03
            Original ID  :: gnl|Prokka|KGFAHJCF_331
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5221
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5221
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1969:5220]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1970..5220
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
     cand_cluster    1..5221
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /kind="single"
                     /product="TPP_AA_metabolism"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5221
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_AA_metabolism"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and not (PFL-like or PFL_6_pcresol or
                     PFL_9_tma or Gly_radical or Gly_430_pcre or Gly_8-9_TMA or
                     PFL_acetate1 or PFL_acetate2 or Gly_radical_scatole or
                     PFL_proline or PFL_toluene) and minimum(2, [ALS_ss_C,
                     AlaDh_PNT_C, Arginase, BKACE, BcrAD_BadFG, Beta_elim_lyase,
                     CPSase_L_D2, Cys_Met_Meta_PP, ELFV_dehydrog, GRDB,
                     Glu_syn_central, Glu_synthase, Gly_reductase, HAAA1, HG2B1,
                     HG2B2, HGD-D, HleuD1, HleuD2, HleuS1, HleuS2,
                     Homoserine_dh, ILVD_EDD, IlvC, Lactoyl-CoA1, Lactoyl-CoA2,
                     Lyase_aromatic, MAAL_C, OKR_DC_1_5_lysine,
                     OKR_DC_1_5_ornithine, OKR_DC_1_7_arginine, OKR_DC_1_C,
                     OTCace, PALP, Proline_reductase, Sacchrp_dh_C, TPL,
                     Thr_dehydrat_C, TnaA, Urocanase]) and not (POR or POR_N or
                     PFOR_II))"
                     /tool="gutsmash"
     CDS             complement(3..1469)
                     /locus_tag="ctg331_1"
                     /transl_table=11
                     /translation="MISVLLGAAGAIAANPTGPETMVVQQGNKVTGLVEDQMGPVAGAS
                     VVVKGTTIGTITDIDGKFTLDNVRRGAVIVISFVGLATQEITWNGQSILNVKLSDATQD
                     LDEVVVVAYGTAKKSTFTGSASVVKAEKLEKIMGSGFAEALQGMSAGVNVVNVQGNPGA
                     DARVEIRGIASMSGKADPLYIVDGMPYDGKLNQINPTDIESMTVLKDAAATSLYGSRAA
                     NGVVMITTKRGKSEKPRINFRGGWGTSDNAVKNPKKADPYQQLENTWYALYYDALLYDG
                     MNMQQAGDYASSQALTKQVKATKDSNGNLIYVTPFRHINENYVLHDGNGNPYMNPNLEY
                     VWDEDDWDVYKAIFSRKLRQDYSVDISGQANGGKTAYFFSGGYLDDQGYGNRQYYKRYS
                     FRTNLSTELFDWWKVAGSLSYSRHRQNLSGGASRAANFTTTLSSPWLRNEDNTGWVVSE
                     KTGERMYSYGKYTNNFFGAHILNNSGDYWDN"
     CDS             complement(1970..3016)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: IlvC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) IlvN"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg331_2"
                     /sec_met_domain="IlvN (E-value: 6.1e-49, bitscore: 154.2,
                     seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="IlvC (E-value: 1.4e-44, bitscore: 140.3,
                     seeds: 295, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAVMNFGGVDENVVTREEFPLEKAREVLKDETIAVIGYGVQGPGQ
                     SLNLRDNGFNVIVGQRKGGKTWEKAVADGWVPGETLFDIEEACEKATIIQYLLSDAAQI
                     EVWPRIKKYLTPGKALYFSHGFGITYKERTNIIPPADVDVILVAPKGSGTSLRRMFLQG
                     RGLNSSYAIFQDATGKAWDRVIALGIGVGSGYLFETTFKKEVYSDLTGERGTLMGAIQG
                     LLLAQYETLRENGHEPSEAFNETVEELTQSLMPLFAENGMDWMYANCSTTAQRGALDWM
                     GPFHDATKPVFQKLYSEVACGNEAQRSIDTNSKPDYREGLEKELAALRESEMWRAGAVV
                     RKLRPENN"
     CDS             complement(3058..3789)
                     /locus_tag="ctg331_3"
                     /transl_table=11
                     /translation="MEKNKVGEFHFVTESYLTDFRGRITIPMIGTYLLHAASCHAADRG
                     FGYNDMTERHTAWVLSRLAIEMTSYPAMSESVTLYTWVDEVGKLFTSRCFELVNGEGKT
                     FGFARSIWAAIDMETRRPTLLDVEGLSAYITDRPCPIEKPGKIAPVENNTSGEPYRVKY
                     SDLDVNGHLNSIKYMEHLLDLFDISLFREKEISRFEIAYQSEGKYGMELTLHNQEVSLD
                     KYNMAICNDGKAISRAAVTWI"
     CDS             complement(3826..4380)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ALS_ss_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg331_4"
                     /sec_met_domain="ALS_ss_C (E-value: 5.6e-19, bitscore:
                     56.7, seeds: 685, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTKQLYTVIIFSENTVGLLNQITVIFTRRQLNIETLSVSPSAIK
                     GIHKFTITTFADEDTIDKVVKQIDKRVDILKAYYNTDEDLVYQEIALYKLSTELFIKMG
                     TVEDLIRKYNARILEMNEDCVVLEKSGHYDETQALFKELSETIGVLQFIRSGRVAITKS
                     KVERLSDMLSAMEKKLQEKHV"
     CDS             complement(4393..5220)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg331_5"
                     /sec_met_domain="TPP_enzyme_C (E-value: 3.5e-52, bitscore:
                     164.7, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LKTYAKQAKIIHLDIDNSEIGKNVPVDVKVLGNAKHTIPMITALL
                     EERKRPEWNAEFEPDEKEEYNKVIEKELYPTEGQLKMGEVVRKVSEATDNDAILVTDVG
                     QNQMMGVRYFKYKQTRSVVTSGGLGTMGFGLPAAIGAKLGAPDRTVCFFTGDGGIQMTI
                     QELGTIMQENLNVKIIVLNNNFLGMVRQWQELFFNERYSNTIMKNPDFVAIAKAFGIAS
                     RAVDKREDLDEAIAEMLNHDGAYMLVANVETCGMVYPMVPAGGSVTNMILGDK"
ORIGIN
        1 ggattatccc aataatcacc cgagttattc aggatatggg caccaaagaa gttattggta
       61 tatttaccgt aactgtacat ccgttcccct gttttttcag aaacgaccca accggtatta
      121 tcttcattac gtaaccaggg tgaagacaaa gtcgtcgtaa agttagccgc acggctggca
      181 ccaccggaaa ggttctggcg atgacgcgaa taagaaaggc taccggccac tttccaccaa
      241 tcgaacaact ctgtactcag gttggtacgg aaagaataac gtttataata ctgccggtta
      301 ccatatccct ggtcgtccag ataaccgccg gagaagaaat aggctgtttt tccaccattg
      361 gcctgaccac ttatatccac actataatcc tggcgcagtt tacgggagaa gatagctttg
      421 tagacatccc aatcatcttc atcccagaca tattccagat taggattcat gtaaggattc
      481 ccattgccgt catgcaatac gtaattttca ttgatatgtc tgaacggagt gacataaatc
      541 agattaccat tcgaatcttt tgtagccttt acctgtttgg tcaatgcctg cgacgaagca
      601 taatcgcccg cctgttgcat attcatgccg tcataaagaa gggcatcata atacaaggca
      661 taccaggtat tttccagctg ctggtacgga tcggcttttt tcggattctt gacagcatta
      721 tccgaagtac cccaacctcc gcggaagtta atacgcggtt tttctgattt accccgcttg
      781 gttgtaatca ttacgacgcc gtttgctgca cgcgaaccgt agagcgaagt ggccgccgca
      841 tccttcagca ccgtcatcga ttctatgtcc gtaggattga tctgattcag ttttccatcg
      901 taaggcatcc cgtccacaat atataacgga tcggcctttc cggacatgga agcaatacca
      961 cggatctcca cacgggcatc agctccgggg ttaccctgta cattcacaac attcacaccg
     1021 gcactcatcc cctgcaatgc ttccgcaaag ccggagccca tgatcttttc gagtttttca
     1081 gcttttacaa cggaggccga tccggtgaag gttgatttct tagccgttcc ataggctacg
     1141 acaacgactt catccaggtc ctgtgtcgca tcacttaatt ttacatttaa aatagattgt
     1201 ccgttccaag tgatctcctg agtggccagg ccgacaaagg aaataacaat aaccgctccc
     1261 cgccgaacat tatccagcgt aaactttcca tcgatatcgg ttatcgtacc gatagtggtt
     1321 cctttcacta caacagaagc tccggcgaca ggtcccatct ggtcctctac caggccggtt
     1381 accttattac cctgttgtac taccatcgtt tccggacctg taggattggc agcaatcgca
     1441 cctgccgcac ctaataatac agaaatcatt agtgatctaa ttagttttga gttggtccac
     1501 atagctaaat tcaagtcttt aattaaataa aattaatcac aatagcgttc atttcattag
     1561 aggttacact tttctaagat aaaagtgact ggcatacatt tcaataaaat aaagttaact
     1621 aaaacaaaga tatactgatc attacacaca acatccttct atatttccac tctacaaact
     1681 acagtatctc catcagcaaa tataacttat cattatttaa tacaagcata attatgtata
     1741 tttattgctc taataaatat attttaacca ccatttaatt gattttttta tctaaaatcc
     1801 acattcaaac aatgacatac atattgggat gaaaaatcaa taacagttgc aatcaaaaac
     1861 attttttaac cattaacgca ttacataaac cataaaaaac ggcaacccga aaagccggat
     1921 tgccgttttt atatcacaaa atgtttaact attaatgtat caccatttat tagttgtttt
     1981 caggacgaag tttacgaaca acagcacctg cacgccacat ttcactttcg cgaagagcag
     2041 ccagttcttt ttccagacct tcacgatagt caggcttgct gtttgtgtcg atagaacgct
     2101 gtgcttcgtt accgcaggca acttcgctat acaacttctg gaacaccggt tttgtagcat
     2161 cgtggaacgg gcccatccag tccaaagcac cacgctgtgc agttgttgaa cagtttgcat
     2221 acatccagtc cataccgttt tctgcgaaca acggcatcaa tgactgagtc agttcttcta
     2281 ctgtttcatt gaatgcttca gaaggttcgt gaccgttttc acgcaaagtt tcgtactgag
     2341 ccaacagtaa tccctggata gctcccatca aagtaccacg ttcgccggtc aggtcagaat
     2401 aaacttcttt cttgaatgtt gtttcaaaca agtaaccaga accaacaccg atacccagtg
     2461 caatcacgcg atcccatgct ttgcctgttg catcctggaa gattgcgtag cttgagttca
     2521 gaccacgacc ctgcaagaac atacggcgca gtgaagtacc tgaacctttc ggagcaacca
     2581 ggattacgtc tacatcagca ggagggatga tgtttgtacg ttctttgtaa gtgataccga
     2641 atccgtgaga gaaatacaaa gccttacccg gagtcaggta ttttttgatg cgcggccaaa
     2701 cttcaatctg agcagcatct gaaagcagat actggatgat agttgctttt tcgcatgctt
     2761 cttcgatatc gaacagtgtt tcgcccggta cccaaccgtc agcaacggcc ttttcccagg
     2821 tcttaccacc tttacgttgt cctacaatta cgttaaaacc gttgtcgcgc aggttcaggc
     2881 tctggccagg accttgtacg ccgtaaccta ttacagcgat tgtttcgtct ttcaatactt
     2941 ctcttgcctt ttccaacgga aattcttcgc gggttactac gttttcgtca acgccgccaa
     3001 aattcattac tgccattttc tttttactat taaaatgtat ataaaataat ttgtttttca
     3061 gatccaggta acggcagcgc ggctgatggc tttgccgtcg ttacagattg ccatattata
     3121 tttatcgagg gatacttcct ggttatggag agtcagctcc atcccatact ttccttcgga
     3181 ctgataagct atttcgaaac ggcttatctc tttttctcgg aacagactga tatcgaacaa
     3241 gtcgagcagg tgttccatgt atttgatact gttcaggtgg ccgtttacgt ccaggtcact
     3301 atacttcaca cggtaaggtt cgccggaagt gttgttttcc accggagcaa ttttacccgg
     3361 tttttcaatc ggacagggac ggtcggtgat ataagcactc aggccctcca catcgagcaa
     3421 ggtcgggcgg cgggtttcca tatcgatagc cgcccagata gagcgggcaa agccaaaggt
     3481 cttgccttcg ccgttcacca gttcaaagca acggctggta aacaacttac cgacctcatc
     3541 cacccaggta tataaagtta cggattcaga catggcagga taagaagtca tctcgatagc
     3601 cagacgggaa agaacccagg cggtatgcct ttcggtcata tcgttatatc cgaatccacg
     3661 gtcggcggca tggcaagaag cagcatgcaa caaataagtt ccgatcatag ggattgtaat
     3721 acgtccccgg aagtccgtca gataagactc tgttacgaaa tgaaattctc ctactttgtt
     3781 cttctccata atttacttta tttacaaaat gtttccttat tattctcaga catgtttttc
     3841 ctgcaacttc ttttccattg cagataacat gtcgctcaga cgttctacct tactcttcgt
     3901 tatagcaacg cgtccggaac ggataaattg caggacaccg atcgtctcac tcaattcttt
     3961 aaacaaagcc tgtgtctcat cataatgacc actcttttcc aacaccacac aatcctcatt
     4021 catttcaagg atacgggcat tgtacttacg gatcagatcc tcaaccgtcc ccatcttgat
     4081 aaagagttcg gtactcagtt tataaagagc gatttcctgg taaaccaggt cttcatccgt
     4141 attataatag gctttcagga tatccacacg cttgtcgatc tgttttacga ccttgtcgat
     4201 cgtatcctcg tcggcaaagg tagtaattgt aaacttatgt attcccttta tagcagaggg
     4261 agaaacagaa agcgtctcaa tgttcagttg ccggcgcgta aagataacag ttatctgatt
     4321 cagaagacca acagtgtttt ccgaaaagat aataacggta tataattgtt ttgtattcat
     4381 agtttctcct ccttacttat cacccaaaat catgtttgtc acgcttcctc cggccggaac
     4441 catcggatat accataccac aagtttccac attggcaacc aacatatagg caccatcgtg
     4501 attcaacatt tcagcaatcg cctcatccaa gtcttcccgt ttatctaccg cacgggaagc
     4561 aataccaaaa gccttggcta tcgctacaaa atccggattc ttcatgatgg tattcgaata
     4621 ccgttcgttg aagaacagct cctgccactg gcgtaccatt cccaggaaat tgttgttaag
     4681 aacgatgatc tttacattca gattttcctg catgatagtt cccaactcct ggatagtcat
     4741 ctggatacca ccgtcgccgg taaagaaaca gaccgtacgg tcaggcgctc ccagcttcgc
     4801 tccaatggcg gcgggaaggc caaagcccat cgttcccaaa cctcctgaag taactacgct
     4861 gcgtgtttgt ttatatttga aatagcgaac acccatcatc tggttctgac ctacatccgt
     4921 aaccagaatc gcatcattat cggttgcttc ggaaacctta cggactactt cgcccatttt
     4981 cagttggcct tcggtaggat agagttcttt ttctattacc ttattatatt cctctttttc
     5041 atccggttcg aattcagcat tccattccgg acgcttccgt tcttccagta aagccgtaat
     5101 catcggaatc gtatgcttgg catttcccag cactttcacg tcgaccggca cattctttcc
     5161 gatctccgaa ttgtcgatat ccaggtggat aatctttgcc tgcttggcat aagtcttcag
     5221 g
//
