LOCUS       c00091_gnlPro..         7400 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|JEEDFCII_91.
ACCESSION   c00091_gnlPro..
VERSION     c00091_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 08:52:17
            Original ID  :: gnl|Prokka|JEEDFCII_91
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7400
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7400
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1223:4179]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1224..4179
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7400
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7400
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(2..313)
                     /locus_tag="ctg91_1"
                     /transl_table=11
                     /translation="MKSTFKVLFYLKKGSEKKNGEVMIMARITIDGKLCQFSTKQSIQP
                     DNWNTAAGKAKGRDAGRINALLDDIRSSLNTIYHEMQRRDNYVTAEKVKNEFLGHSESH
                     "
     CDS             1224..3452
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg91_2"
                     /sec_met_domain="Gly_radical (E-value: 2.8e-47, bitscore:
                     148.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1171.1, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKTFTNGLWNKEINVSDFVSKNIAPYTGDASFLQGPTERTKR
                     IWDICLKALEEERNNNGVRSLDNTTVSTITSHKAGYIDKDNELIVGLQTDELLKRSIKP
                     FGGINVVAKACRENGVEVDDKVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGIDRLIEAKQEDLRNLTGPMTEARIRLREEVAEQIKALKDIK
                     VMGEYYGLDLSHPATSAQEAVQWVYMAYLAAIKEQDGAAMSLGNVSSFLDIFIEYDLAH
                     GKINETFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTEAIGGRFNDGRVKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKDFCAKVSVDTSSIQYENDNLMREVRNC
                     DDYGIACCVSYQAIGKQIQFFGARANLAKALLLAINGGRCENTGTVMVKGIPVLTGETL
                     KFEEVMANYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMAFIDTNPRINLAYGVAGL
                     SIAIDSLSAIKYAKVTARRNEIGLTEGFDTEGSFPCFGNNDDRVDHLGVDLVYYFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGPTPEERVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLEDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             3454..4179
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 75.5;
                     E-value: 1.7e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg91_3"
                     /sec_met_domain="Radical_SAM (E-value: 9.2e-32, bitscore:
                     99.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCPFRCLYCANPDTIDVKGG
                     TPTTPEEILQMAISQKAFFGKKGGITFSGGEPTLQAEALIPLFKELKANGIHICLDSNG
                     GIWNEKVEELFSLTDLVLLDIKQFNPERHRTLTGRSNEQTLRTAAWLEEHEHPFWLRYV
                     LVPGYSDSEEDIRLLGESLGKYRQIQRVEILPYHRLGVHKYEVMGWDYKLKEVKENTPE
                     QLERAEKLFKEYFTNVITN"
     CDS             complement(4248..6452)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fn3-like"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3_C"
                     /gene_functions="other (smcogs) SMCOG1041:Fibronectin type
                     III-like domain (Score: 87; E-value: 2.2e-27)"
                     /gene_kind="other"
                     /locus_tag="ctg91_4"
                     /sec_met_domain="Fn3-like (E-value: 1.8e-28, bitscore:
                     87.0, seeds: 729, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3 (E-value: 1.8e-75, bitscore:
                     242.6, seeds: 11, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3_C (E-value: 3.4e-53,
                     bitscore: 169.1, seeds: 116, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKIIILMVCLWGLGNTFTSAQTTEKEKFISSLMKQMTLDEKIGQ
                     LAQCTYRGNFTGPDGGNIPKEEYVRQGRIGSMLNVIGVKDIRRYQELALQSRLRIPLIF
                     GLDVIHGYRTGFPLPLAEAASFDLAMIEEAARYNALESAADGLNWVFAPMVDISWDARW
                     GRVMEGAGEDPYYGSRVAEARVRGLQGDDLADTTTVMACMKHFAGYGAPIAGKEYNSVD
                     MSMGHFANFYMPPYKAAIKVGAATVMSAFNDFNNIPCTANDFLLNRLLREQWGFKGFVV
                     SDYNSVEELVNHRYAVDKKDAAAKALNAGLDMEMVSTCYLTYLKELVQEGKVKESVLDD
                     AVRRILEKKYELGLFEDPFRYCNPERAARILNAPEVRNASLRMAERSVVLLENRESVLP
                     LTAQTRKIALIGGLSKSQYDMAGAWAATTDRSKVVTLYDALVRKGLEVNYAEGYDMKSN
                     QLTGLQEALQAAEASDVIVVAVGERFGQSGEKASKVNIAIPEGQQKLVSELAKTGKPVI
                     ALVMCGRPVIFNEIREHADAVLCTWWLGSEAGNAICNVLWGEYNPSAKLPMTFPAHVGQ
                     IPIYYQNKSTGRPTALQQTYKASYIDASTEPAYPFGYGLSYTDFGYSDLKLLPGKDAGV
                     HTEVSVKVTNTGKCAGEEVVQLYVQDKVASVTRPIKELKGFRKIHLNAGESKTVAFQIT
                     DDALGFYDNEMNYMVEPGDFVFMVGGSSDDVQQITYNLKE"
     CDS             complement(6483..7400)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fn3-like"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3_C"
                     /gene_functions="other (smcogs) SMCOG1041:Fibronectin type
                     III-like domain (Score: 82.1; E-value: 7.8e-26)"
                     /gene_kind="other"
                     /locus_tag="ctg91_5"
                     /sec_met_domain="Fn3-like (E-value: 6.3e-27, bitscore:
                     82.1, seeds: 729, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3_C (E-value: 6.8e-40,
                     bitscore: 125.6, seeds: 116, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="TEIYTLSLHDALPIYVGEATVSFQAGMMDEERLASSLKHARNVIV
                     CAGFNSSTEGEGFDRPFALSYGQEYLINKVASLHDNVAVVVNAGGGIDFRNWGQSVQAI
                     LMAWYPGQEGGKALAEIITGKLSPSGKLPVSIEEKWEDNPVYGNYYDNRNVPHKRVQYA
                     EGVFVGYRGYDRNGKQPLYPFGYGLSYSSFEYSNLSVEKTGGSRVTVSFDIKNTGKMDA
                     AEAAQVYVRDVECSVPRPLKELKGYDKVYLKKGETKRVRILLDEEAFAYYDVESHRFVV
                     EKGTFEILAGPSSADLPLKATVVL"
ORIGIN
        1 cgtggctctc gctatgacct aaaaactcgt tcttcacttt ctcggcggtc acgtagttgt
       61 cacgccgttg catttcgtgg taaatggtgt tcagggaaga acgtatgtcg tccaaaaggg
      121 cgtttatcct cccggcatcc ctgcctttgg ctttgcccgc agccgtgttc cagttgtcgg
      181 gctggatgct ttgtttcgta ctgaactggc aaagtttgcc gtctatggtg atgcgtgcca
      241 taatcataac ctcgccgttt ttcttttcag aacctttctt caaataaaaa agaaccttga
      301 atgtcgattt cataactcac tttttttagt tgcaaaatta atttttacct ctcgttttcg
      361 agttatgcag actatggact aatcaagtca ttttcaggtc atttgaggac aaatcgttac
      421 cgtttttgtc cggcagggaa taggtaacga tttagtaacg aaactttgtc ttttaccgta
      481 cttttgaagt cttggaagca ggactttcgg gcaataaaaa aagccacaaa tcgttgattt
      541 gtagcttttt gtccgtttgc tgaccatttc tgtccagcac gttcagcgga gagacaggga
      601 ttcgaacccc gggtgcctcg cagcacaacg gttttcaaga ccgccgcaat cgaccactct
      661 gccacctctc caaaacttct ttttcagaag tgcttttctt ttaaagcggt gcaaagatac
      721 aaatcatttt ttattctaca aacttttctg cacaaaaaca gagcaattat accaaaattc
      781 ttgtttatac ccactacgga acgaacataa cggcatttag gctatttgtt gaaatttatc
      841 aagggcaaga cagagtttta taacttactg gctatcagta tatttacaaa gaaccacatt
      901 cagtatacga ttgaacccta ttcagtatac gactgaatgg cattcactat acgactgaat
      961 taggttcaga atacgactgt tccttaaacc tacatcattt gtaaataatg cagattatta
     1021 ttcggttctt gtttcaattc ataaaccaca tatagaaagt attaacatct ctactccccc
     1081 ggacattcac tacctaataa tagttatttt atgctgttca acataaaaag agaagtatct
     1141 cttataaaat tacggaattt attcagtatt tttgtgcgct ctaaaggata acattatcta
     1201 acctactaac aaaaacaaat tttatggaat tgaacaaaac ctttaccaat gggctttgga
     1261 ataaagaaat caatgtcagt gatttcgtca gtaaaaacat cgcaccctat accggggatg
     1321 catctttctt gcagggacct accgaacgta ccaaacgtat ctgggacatc tgtctgaagg
     1381 ctctcgaaga agaaaggaac aacaatggcg tccgttcact ggacaatacc accgtatcta
     1441 ccatcacttc tcataaagcc gggtatattg ataaggacaa tgaacttatt gtcggtttgc
     1501 agactgatga actgttgaaa cgatccatca aacctttcgg aggcatcaac gtggtagcaa
     1561 aagcatgccg tgaaaacgga gtggaagtgg acgataaagt aaaagacatc ttcacccatt
     1621 accgcaaaac acataacgac ggcgtattcg atgtatatac cgaagaaatc cgttccttcc
     1681 gctcactggg attccttacc ggattgcccg acaactatgc ccgcggacgt atcatcggcg
     1741 actaccgccg attggctctc tacggcatcg accgtctgat tgaagccaag caggaggact
     1801 tgcgcaacct caccggcccg atgaccgaag cacgcatccg tctacgcgaa gaagtggcgg
     1861 aacaaatcaa agctttgaag gacatcaagg ttatgggcga atattacgga ctcgacctca
     1921 gccatccggc tacatcagct caagaggccg tgcaatgggt atacatggca tatcttgctg
     1981 ccatcaaaga gcaagacggt gctgccatgt cattgggtaa cgtatcttcg ttcctcgata
     2041 tattcattga atacgatctg gctcacggaa aaatcaatga gacgtttgcg caagaactga
     2101 tagaccagtt cgtcatcaag ctgcgcatgg tacgccatct gcgtatgcag tcgtacaatg
     2161 atatctttgc cggtgacccg acttgggtga ccgaagccat cggaggacgc ttcaatgacg
     2221 gacgcgtgaa agtcaccaag acttctttcc gcttcttgca gacgctatac aacctcggtc
     2281 cttctccgga accaaacttg accgtactgt ggagtcccga actgccggaa ggcttcaagg
     2341 atttctgcgc caaagtttcc gtagatacca gctccatcca gtacgaaaac gacaacctga
     2401 tgcgcgaagt gcgcaactgc gacgattacg gcattgcctg ctgtgtatcc taccaagcca
     2461 tcggcaagca aatccagttc ttcggcgcac gtgccaacct tgccaaggcc ctgctgcttg
     2521 ccatcaacgg tggccgttgc gaaaacaccg gaactgtcat ggtgaaaggt attcccgtat
     2581 tgaccggcga gacactgaag tttgaggaag ttatggccaa ctacaagaag gtactgactg
     2641 aaatagcccg tgtctacaac gaggccatga atatcatcca ctacatgcat gataagtatt
     2701 attatgagaa ggcccagatg gcattcatcg ataccaaccc acgcatcaat ctggcatacg
     2761 gtgttgccgg cctttccatc gccatagact cactgtccgc catcaaatat gccaaagtca
     2821 ctgcacgccg caacgaaatc ggcctgaccg aaggtttcga cactgaaggc tcattcccct
     2881 gcttcggcaa taatgacgac cgtgtggacc acctgggagt agacctcgtt tactatttca
     2941 gcgaagaact gaaaaagctg cctgtctaca agaatgcccg tcccaccctt tccttgctga
     3001 ctatcacctc caacgtgatg tacggcaaga agaccggtgc cactccggac ggacgcgcca
     3061 aaggtgttgc ctttgctccg ggtgccaacc caatgcacgg acgtgacaag aacggtgcca
     3121 tcgcttcctt gagctccgta gccaaattgc gctaccgcga ctcgcaggac ggcatcagca
     3181 atacgttctc cattgttcct aaatcactgg gcccgacacc ggaagaacgt gtagagaacc
     3241 tggtaaccat gatggacggc tacttcacca aaggtgccca ccatttgaac gtaaacgtac
     3301 tgaaccgcga aatgctggaa gatgccatgg aacatcctga aaagtatccg caactcacca
     3361 tccgcgtttc cggctatgcc gtgaacttcg tgaagttgag ccgcgagcat cagttggaag
     3421 tcatcagccg ttcattccac gaaagaatgt aatatgataa atgtacactc ctacgaaagt
     3481 atgggaacat tcgacgggcc gggtctccgg ctcgtcgttt tcctgcaagg atgtccgttc
     3541 cgttgtctgt attgtgccaa tccggatacg attgatgtaa aaggaggcac tcccaccact
     3601 ccggaagaga ttctgcaaat ggctatcagc caaaaggcat tcttcgggaa gaaaggaggt
     3661 attactttct ccggcggtga gcctaccctg caagcagaag cgctgattcc tcttttcaag
     3721 gagctgaagg cgaacggcat ccatatctgt ctggacagta acggaggcat ctggaatgaa
     3781 aaggtggaag agctctttag cctgactgac ctcgtgctgc tggacatcaa gcaattcaat
     3841 cccgaacgcc accgtacgct gacgggacga agcaacgaac agacactccg caccgctgcc
     3901 tggctcgaag aacatgaaca ccccttctgg ctgcgttatg tactggttcc cggttacagc
     3961 gattcggaag aagacatccg cctgctgggt gaaagtctcg gcaaataccg gcagatacaa
     4021 cgggtggaaa tacttcctta ccaccgtctg ggcgttcaca aatacgaagt catgggatgg
     4081 gattacaaat tgaaagaagt gaaggaaaat actccggaac aactggaacg ggcagaaaaa
     4141 ctgttcaaag agtatttcac gaatgtgata accaactaaa acagaaaagc aaggctgtcc
     4201 ggatatgatt ccagacagcc ttgcttttca tttataggac aagaatccta ttctttcaag
     4261 ttataggtaa tctgctgtac atcatcacta ctgccaccca ccataaagac gaaatcacca
     4321 ggttctacca tataattcat ctcattgtca tagaatccca gtgcatcgtc agttatctga
     4381 aaagctacag ttttgctttc acccgcattc agatgaattt tcctgaagcc cttcaactcc
     4441 tttatcgggc gcgtaacaga cgctacctta tcctgcacat acaactggac tacttcctct
     4501 cctgcacact taccggtatt agtcactttt accgacacct ccgtatggac gcctgcatcc
     4561 ttgcccggca gcagtttcaa atcagaatat ccaaaatccg tatacgaaag cccatatccg
     4621 aaaggatagg caggctcagt ggaagcatcg atataggatg ccttataagt ctgttgcaat
     4681 gcggtaggac gaccggtact cttattctgg taataaatgg gaatctgccc tacgtgtgca
     4741 ggaaatgtca tgggcaattt agccgaagga ttatactctc cccacaacac attgcatatg
     4801 gcattgccgg cctcactccc cagccaccaa gtacacagta ccgcatcagc atgttccctt
     4861 atctcattga agattaccgg acgcccacac atgacaagag caatgacagg ttttccagtc
     4921 tttgccagtt ccgacaccag cttctgctgc ccctcgggta tggctatatt cactttggaa
     4981 gccttctcgc cactctgacc gaatctctcg cctaccgcca caacaatgac atccgaagcc
     5041 tcagcagcct gcaatgcttc ctgcagtccg gtcaattgat tgctcttcat atcatagcct
     5101 tcagcatagt tcacctccag tcctttccgg accagtgcat catacaaggt caccactttg
     5161 ctccggtccg tagtggcggc ccaagcgcct gccatatcgt attgggattt tgagagtcct
     5221 cctatcaagg ctatcttcct cgtctgtgcc gtcaacggaa gcacagattc cctattctcc
     5281 agcaatacaa cggaacgctc ggccatacgg agcgaagcat tacggacttc gggcgcattg
     5341 agtattctgg ccgctctttc cggattacag tagcgaaaag ggtcctcaaa caatcccaac
     5401 tcatatttct tctccaatat acgccgtact gcatcatcaa gcacactctc ctttactttc
     5461 ccttcttgaa caagctcctt cagataagtc aagtagcagg tacttaccat ctccatgtcc
     5521 agaccagcat tcagcgcttt ggctgcggca tctttcttgt caacagcata acggtggttc
     5581 accaattcct ctaccgaatt ataatcggac acaacaaatc ccttaaatcc ccactgctct
     5641 ctcaatagcc ggttgagcaa gaaatcattg gcagtacaag gaatattatt aaaatcattg
     5701 aaagcactca tcacggtagc tgctcctacc tttatggccg ccttataggg aggcatataa
     5761 aaattggcaa agtggcccat tgacatatcc acactattat actcctttcc tgctatcggt
     5821 gcaccatatc cggcaaaatg tttcatacat gccatgacag tcgtcgtgtc tgccaaatcg
     5881 tctccttgca agcctctgac acgcgcctcc gctacccgag aaccataata agggtcctca
     5941 ccggcccctt ccatgacgcg tccccaacgt gcgtcccacg aaatatccac catcggggca
     6001 aaaacccagt tcaaaccatc ggcggcactt tccaaagcat tgtagcgtgc agcttcctca
     6061 atcatggcca aatcgaaact tgcagcctcg gcaagcggaa gcggaaatcc ggtacgatag
     6121 ccgtggataa catccagtcc gaaaattaaa ggaatgcgca aacgcgattg caaagcaagc
     6181 tcttgataac ggcgtatatc ctttactcct atcacattca gcatagagcc tattctgcct
     6241 tgccggacat actcttcttt aggaatattt ccaccgtcgg gtccggtaaa attccctcgg
     6301 taggtgcatt gtgcaagctg gcctatcttt tcatccagcg tcatctgctt catcaagctt
     6361 gagatgaatt tctccttctc cgtagtttgt gccgaggtaa aggtgttacc aagtccccac
     6421 agacaaacca tcagtattat tatctttttc atactgccat tcttgttttg atatttattt
     6481 tatcataaaa caacagttgc cttcaatggt aaatcagctg aagaaggacc tgccagaatc
     6541 tcaaaagtcc ccttttccac tacaaaccga tgtgattcca catcatagta ggcaaaggct
     6601 tcctcatcca gcaatatccg aacgcgcttg gtttcaccct tcttcaagta aaccttatcg
     6661 tagcctttca actccttcaa tggacgcgga acagaacatt ccacatcccg cacatatacc
     6721 tgagccgctt ccgcagcatc catctttccg gtattcttga tgtcaaagct taccgtaacc
     6781 cggcttcccc ccgtcttctc aacactcaga ttactatatt caaaactact gtatgacagt
     6841 ccatacccga aaggataaag cggctgcttt ccgttccggt catatcccct ataacctacg
     6901 aaaacacctt cggcatactg cacccgttta tgaggtacat tgcgattgtc ataataatta
     6961 ccatataccg gattatcttc ccatttttcc tcgatgctga ctggtagttt gccacttggc
     7021 gaaagcttgc ccgtaatgat ttcggcaagg gcctttccac cctcctgccc cggataccat
     7081 gccatcaaaa tagcttgtac actctgcccc caattgcgga aatctattcc acctccggca
     7141 ttcaccacca cagccacatt atcatgaagt gaagccactt tattaatgag atattcctgc
     7201 ccgtatgaca atgcaaacgg acggtcgaac ccttcacctt ctgtactgct gttgaacccg
     7261 gcacatacaa taacattcct ggcatgcttc agtgaagagg ccaaacgctc ctcatccatc
     7321 attcctgcct ggaaactgac agtcgcctca cccacataga tcggaagagc gtcgtgtagg
     7381 gaaagagtgt agatctcggt
//
