LOCUS       c00277_gnlPro..         2724 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|AKOBPHOC_277.
ACCESSION   c00277_gnlPro..
VERSION     c00277_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 11:46:56
            Original ID  :: gnl|Prokka|AKOBPHOC_277
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2724
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2724
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[745:2722]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      746..2722
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..2724
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2724
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(2..547)
                     /locus_tag="ctg277_1"
                     /transl_table=11
                     /translation="MKTPQEIAEMISKRFYPLPDADLDSLASIIESSKAAKGELLLDNG
                     QISKYLYYVESGLLRQFYYKNKRDITEHFSCEGNIVICIASTFQREPTRLLIEALEPTV
                     YHRISFSEFSDLLKTSLAINRLYQKILEWALMLSQEKADSWRFESARKRYLRFQQEYPE
                     AAKRASVNHIASYLLMTPE"
     CDS             complement(746..1480)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 67.6;
                     E-value: 4.7e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg277_2"
                     /sec_met_domain="Radical_SAM (E-value: 3.8e-34, bitscore:
                     106.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWI
                     RHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPL
                     SKERLDNAKAIFSRYKPENNKA"
     CDS             complement(1481..2722)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg277_3"
                     /sec_met_domain="Gly_radical (E-value: 4.4e-47, bitscore:
                     146.9, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 7.3e-130, bitscore:
                     421.8, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="GVDGRSLVTRTSYRYLHTLYNLGPAPEPNLTVLWFKNAPENWKRF
                     CAKVSIDTSAIQYENDDLMRPDYGDDYGIACCVSPMKIGKQMQFFGARANLAKCLLYAI
                     NGGRDERSGVQVAPMFEPVRGEYLEYDEVMAKYEQMMRWLAKVYVNALKIIHYMHDKYA
                     YEAFEMSLHDGDVERIRATGIAGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYV
                     PFGNNDDRTDSIAVEITEKFMEYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGR
                     IGGTPFAPGANPMNGRDTKGAIAALASVAKLPFQHAHDGISYTFAVSPATLGKEREVQI
                     NNLVSLLDGYFTPDGGQHLNVNVFDKDLLIDAMEHPEKYPQLTIRVSGYAVNFVKLTRE
                     QQLDVISRTINHSL"
ORIGIN
        1 attcgggtgt catcaataaa tacgaggcga tatgattgac agaagcacgt ttcgctgctt
       61 ccggatattc ctgttgaaaa cgcagataac gttttcttgc cgactcaaaa cgccaagagt
      121 ccgctttctc ttgcgaaagc atcaaagccc actccagtat tttctggtac aatctattaa
      181 tagctaaaga tgttttcaat aaatcgctaa attccgaaaa agagatccgg tgataaaccg
      241 tcggttccaa tgcctcgatt aataaacgcg tcggttctct ttggaatgtg ctggctatac
      301 aaataacgat atttccctca caggagaaat gctcggtaat atcacgttta ttcttatagt
      361 aaaactggcg taataatccc gattccacat aatataaata tttagatatt tgtccgttat
      421 ccaacaataa ttctcctttc gcagccttac ttgattctat gatcgaggcc agagaatcca
      481 aatccgcatc gggtaatgga taaaaacgtt tggatatcat ttcggctatt tcttgtggtg
      541 tcttcatact attcatcatt ttctgatcta tatcattttt tatctcctta caaaaataga
      601 tatttgttgc atataacaaa acaaacacat attaaatagt caaggttagt ctaggcaaaa
      661 aattattact atcgtttaaa agctaatcgg attagattgt tttccctgat tagctgttgc
      721 ataatcgcaa ggcttgatcg agagatcagg ccttgttatt ttctggtttg tagcgagaga
      781 atatcgcttt cgcattatct aaacgttctt ttgataaggg ttccaccccc tttaacggat
      841 aatcgagacc taattgctca tatttatagg ttcccatcgt atgataaggc aataattcca
      901 ctttgcgaac cactttatat gaactgacat accgagctaa cgcctccagc cactcgtcat
      961 tatccgtata tccggggaca ataacatggc gaatccaagt atctatgcca cgtctctcca
     1021 attcatccaa gaacagaagc gtattatcct gcttgacacc cgccagtaac ggatgtaaat
     1081 ccgggtttaa ggttttgatg tccaataaca ctaagtccgc ataatccaag acttcccaag
     1141 cttttgaggt acatacgaaa ccagatgtgt ccaaagctgt gtgaagacct tgctcttggc
     1201 acaaacggaa aaactctttc agaaactcag gttgcaataa aggttcccca ccggtaacag
     1261 tcaccccccc acgggcgata aaactcttat accgtaagac ttccgtcaat agctcgccgg
     1321 gagtcatctg atatttcacc ttccctttcg ggttccaagt atccgggtta tggcaataca
     1381 ggcaacgcaa cgggcagccc tgcataaaaa cgacaaagcg aattccggga ccatctacgg
     1441 tcccgaaact ctctaatgaa tgtatcttac cttttatcac ttacagtgaa tggttaatgg
     1501 ttcttgaaat aacatccaac tgttgttcac gagtcagttt cacgaagttt accgcatatc
     1561 cggatacacg gatcgtaagt tgcggatatt tctcaggatg ttccatagcg tcgatcaaca
     1621 aatccttatc aaatacgtta acattcagat gctgacctcc atccggagta aaatagccat
     1681 ccaataagct aaccagatta ttgatctgca cttcacgctc ctttcccaac gtagccggag
     1741 aaacagcgaa agtataagag ataccgtcgt gcgcatgctg gaatggaagc ttcgctaccg
     1801 aagctagcgc agcgatagca cctttcgtat cacgaccgtt catagggttc gcgcccggag
     1861 cgaaaggagt tccaccgata cggccatccg gtgtcgtacc agtcttctta ccatatacca
     1921 cattagaggt gatcgtaagg atggactgcg taggtttcgc atggcgatag gtctcgtgct
     1981 gacgcaaata ttccatgaac ttctcggtga tctctacagc gatactatcc gtacgatcgt
     2041 cgttattacc gaaaggaaca tattcgccct cacgttcgaa atcaaccgcc aagccacgct
     2101 cgtcacggat cacacgtact ttcgtatcac ggatagcagc caacgaatcg gcaacgatag
     2161 aaagaccggc gatacctgtc gcacggatac gctctacatc tccatcgtga agcgacatct
     2221 cgaatgcctc atatgcgtat ttatcatgca tataatggat tatcttcaag gcgttcacat
     2281 acactttcgc caaccagcgc atcatttgct catacttagc catcacctca tcgtattcca
     2341 gatactcgcc gcgaaccggc tcgaacatcg gggctacctg tacgccgctg cgctcatccc
     2401 gtcctccgtt gatcgcatat aataaacatt tcgccaaatt cgcacgggct ccgaagaact
     2461 gcatctgctt accgatcttc ataggagaaa cgcaacaagc gataccataa tcatctccat
     2521 aatccggacg catcaaatcg tcgttctcat attggatagc cgatgtatcg atagagacct
     2581 tggcacagaa acgtttccag ttctcgggag cgttcttaaa ccacagaacc gtcaaattgg
     2641 gttctggtgc cggacctaga ttatataatg tatggagata acgataggaa gttcttgtta
     2701 ccaaagaacg gccatccact cctt
//
