LOCUS       c00227_gnlPro..         5791 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|HKOPHDEH_227.
ACCESSION   c00227_gnlPro..
VERSION     c00227_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 07:00:57
            Original ID  :: gnl|Prokka|HKOPHDEH_227
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5791
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5791
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[599:3555]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      600..3555
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5791
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5791
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             600..2828
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg227_1"
                     /sec_met_domain="Gly_radical (E-value: 1.7e-47, bitscore:
                     148.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1171.1, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKTFTNGLWNKEINVSDFVSKNIAPYTGDASFLQGPTERTKR
                     IWDICLKALEEERNNNGVRSLDNTTVSTITSHKAGYIDKDNELIVGLQTDELLKRSIKP
                     FGGINVVAKACRENGVEVDDKVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGIDRLIEAKQEDLRNLTGPMTEARIRLREEVAEQIKALKDIK
                     VMGEYYGLDLSHPATSAQEAVQWVYMAYLAAIKEQDGAAMSLGNVSSFLDIFIEYDLAH
                     GKINETFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTEAIGGRFNDGRVKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKDFCAKVSVDTSSIQYENDNLMREVRNC
                     DDYGIACCVSYQAIGKQIQFFGARANLAKALLLAINGGRCENTGTVMVKGIPVLTGETL
                     KFEEVMANYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMAFIDTNPRINLAYGVAGL
                     SIAIDSLSAIKYAKVTARRNEIGLTEGFDTEGSFPCFGNNDDRVDHLGVDLVYYFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGPTPEERVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLEDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             2830..3555
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 75.5;
                     E-value: 1.7e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg227_2"
                     /sec_met_domain="Radical_SAM (E-value: 5.5e-32, bitscore:
                     99.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCPFRCLYCANPDTIDVKGG
                     TPTTPEEILQMAISQKAFFGKKGGITFSGGEPTLQAEALIPLFKELKANGIHICLDSNG
                     GIWNEKVEELFSLTDLVLLDIKQFNPERHRTLTGRSNEQTLRTAAWLEEHEHPFWLRYV
                     LVPGYSDSEEDIRLLGESLGKYRQIQRVEILPYHRLGVHKYEVMGWDYKLKEVKENTPE
                     QLERAEKLFKEYFTNVITN"
     CDS             complement(3624..5789)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fn3-like"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3_C"
                     /gene_functions="other (smcogs) SMCOG1041:Fibronectin type
                     III-like domain (Score: 87.1; E-value: 2.2e-27)"
                     /gene_kind="other"
                     /locus_tag="ctg227_3"
                     /sec_met_domain="Fn3-like (E-value: 1.1e-28, bitscore:
                     87.1, seeds: 729, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3 (E-value: 1e-75, bitscore:
                     242.6, seeds: 11, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3_C (E-value: 1.9e-53,
                     bitscore: 169.1, seeds: 116, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LGNTFTSAQTTEKEKFISSLMKQMTLDEKIGQLAQCTYRGNFTGP
                     DGGNIPKEEYVRQGRIGSMLNVIGVKDIRRYQELALQSRLRIPLIFGLDVIHGYRTGFP
                     LPLAEAASFDLAMIEEAARYNALESAADGLNWVFAPMVDISWDARWGRVMEGAGEDPYY
                     GSRVAEARVRGLQGDDLADTTTVMACMKHFAGYGAPIAGKEYNSVDMSMGHFANFYMPP
                     YKAAIKVGAATVMSAFNDFNNIPCTANDFLLNRLLREQWGFKGFVVSDYNSVEELVNHR
                     YAVDKKDAAAKALNAGLDMEMVSTCYLTYLKELVQEGKVKESVLDDAVRRILEKKYELG
                     LFEDPFRYCNPERAARILNAPEVRNASLRMAERSVVLLENRESVLPLTAQTRKIALIGG
                     LSKSQYDMAGAWAATTDRSKVVTLYDALVRKGLEVNYAEGYDMKSNQLTGLQEALQAAE
                     ASDVIVVAVGERFGQSGEKASKVNIAIPEGQQKLVSELAKTGKPVIALVMCGRPVIFNE
                     IREHADAVLCTWWLGSEAGNAICNVLWGEYNPSAKLPMTFPAHVGQIPIYYQNKSTGRP
                     TALQQTYKASYIDASTEPAYPFGYGLSYTDFGYSDLKLLPGKDAGVHTEVSVKVTNTGK
                     CAGEEVVQLYVQDKVASVTRPIKELKGFRKIHLNAGESKTVAFQITDDALGFYDNEMNY
                     MVEPGDFVFMVGGSSDDVQQITYNLKE"
ORIGIN
        1 acaacggttt tcaagaccgc cgcaatcgac cactctgcca cctctccaaa acttcttttt
       61 cagaagtgct tttcttttaa agcggtgcaa agatacaaat cattttttat tctacaaact
      121 tttctgcaca aaaacagagc aattatacca aaattcttgt ttatacccac tacggaacga
      181 acataacggc atttaggcta tttgttgaaa tttatcaagg gcaagacaga gttttataac
      241 ttactggcta tcagtatatt tacaaagaac cacattcagt atacgattga accctattca
      301 gtatacgact gaatggcatt cactatacga ctgaattagg ttcagaatac gactgttcct
      361 taaacctaca tcatttgtaa ataatgcaga ttattattcg gttcttgttt caattcataa
      421 accacatata gaaagtatta acatctctac tcccccggac attcactacc taataatagt
      481 tattttatgc tgttcaacat aaaaagagaa gtatctctta taaaattacg gaatttattc
      541 agtatttttg tgcgctctaa aggataacat tatctaacct actaacaaaa acaaatttta
      601 tggaattgaa caaaaccttt accaatgggc tttggaataa agaaatcaat gtcagtgatt
      661 tcgtcagtaa aaacatcgca ccctataccg gggatgcatc tttcttgcag ggacctaccg
      721 aacgtaccaa acgtatctgg gacatctgtc tgaaggctct cgaagaagaa aggaacaaca
      781 atggcgtccg ttcactggac aataccaccg tatctaccat cacttctcat aaagccgggt
      841 atattgataa ggacaatgaa cttattgtcg gtttgcagac tgatgaactg ttgaaacgat
      901 ccatcaaacc tttcggaggc atcaacgtgg tagcaaaagc atgccgtgaa aacggagtgg
      961 aagtggacga taaagtaaaa gacatcttca cccattaccg caaaacacat aacgacggcg
     1021 tattcgatgt atataccgaa gaaatccgtt ccttccgctc actgggattc cttaccggat
     1081 tgcccgacaa ctatgcccgc ggacgtatca tcggcgacta ccgccgattg gctctctacg
     1141 gcatcgaccg tctgattgaa gccaagcagg aggacttgcg caacctcacc ggcccgatga
     1201 ccgaagcacg catccgtcta cgcgaagaag tggcggaaca aatcaaagct ttgaaggaca
     1261 tcaaggttat gggcgaatat tacggactcg acctcagcca tccggctaca tcagctcaag
     1321 aggccgtgca atgggtatac atggcatatc ttgctgccat caaagagcaa gacggtgctg
     1381 ccatgtcatt gggtaacgta tcttcgttcc tcgatatatt cattgaatac gatctggctc
     1441 acggaaaaat caatgagacg tttgcgcaag aactgataga ccagttcgtc atcaagctgc
     1501 gcatggtacg ccatctgcgt atgcagtcgt acaatgatat ctttgccggt gacccgactt
     1561 gggtgaccga agccatcgga ggacgcttca atgacggacg cgtgaaagtc accaagactt
     1621 ctttccgctt cttgcagacg ctatacaacc tcggtccttc tccggaacca aacttgaccg
     1681 tactgtggag tcccgaactg ccggaaggct tcaaggattt ctgcgccaaa gtttccgtag
     1741 ataccagctc catccagtac gaaaacgaca acctgatgcg cgaagtgcgc aactgcgacg
     1801 attacggcat tgcctgctgt gtatcctacc aagccatcgg caagcaaatc cagttcttcg
     1861 gcgcacgtgc caaccttgcc aaggccctgc tgcttgccat caacggtggc cgttgcgaaa
     1921 acaccggaac tgtcatggtg aaaggtattc ccgtattgac cggcgagaca ctgaagtttg
     1981 aggaagttat ggccaactac aagaaggtac tgactgaaat agcccgtgtc tacaacgagg
     2041 ccatgaatat catccactac atgcatgata agtattatta tgagaaggcc cagatggcat
     2101 tcatcgatac caacccacgc atcaatctgg catacggtgt tgccggcctt tccatcgcca
     2161 tagactcact gtccgccatc aaatatgcca aagtcactgc acgccgcaac gaaatcggcc
     2221 tgaccgaagg tttcgacact gaaggctcat tcccctgctt cggcaataat gacgaccgtg
     2281 tggaccacct gggagtagac ctcgtttact atttcagcga agaactgaaa aagctgcctg
     2341 tctacaagaa tgcccgtccc accctttcct tgctgactat cacctccaac gtgatgtacg
     2401 gcaagaagac cggtgccact ccggacggac gcgccaaagg tgttgccttt gctccgggtg
     2461 ccaacccaat gcacggacgt gacaagaacg gtgccatcgc ttccttgagc tccgtagcca
     2521 aattgcgcta ccgcgactcg caggacggca tcagcaatac gttctccatt gttcctaaat
     2581 cactgggccc gacaccggaa gaacgtgtag agaacctggt aaccatgatg gacggctact
     2641 tcaccaaagg tgcccaccat ttgaacgtaa acgtactgaa ccgcgaaatg ctggaagatg
     2701 ccatggaaca tcctgaaaag tatccgcaac tcaccatccg cgtttccggc tatgccgtga
     2761 acttcgtgaa gttgagccgc gagcatcagt tggaagtcat cagccgttca ttccacgaaa
     2821 gaatgtaata tgataaatgt acactcctac gaaagtatgg gaacattcga cgggccgggt
     2881 ctccggctcg tcgttttcct gcaaggatgt ccgttccgtt gtctgtattg tgccaatccg
     2941 gatacgattg atgtaaaagg aggcactccc accactccgg aagagattct gcaaatggct
     3001 atcagccaaa aggcattctt cgggaagaaa ggaggtatta ctttctccgg cggtgagcct
     3061 accctgcaag cagaagcgct gattcctctt ttcaaggagc tgaaggcgaa cggcatccat
     3121 atctgtctgg acagtaacgg aggcatctgg aatgaaaagg tggaagagct ctttagcctg
     3181 actgacctcg tgctgctgga catcaagcaa ttcaatcccg aacgccaccg tacgctgacg
     3241 ggacgaagca acgaacagac actccgcacc gctgcctggc tcgaagaaca tgaacacccc
     3301 ttctggctgc gttatgtact ggttcccggt tacagcgatt cggaagaaga catccgcctg
     3361 ctgggtgaaa gtctcggcaa ataccggcag atacaacggg tggaaatact tccttaccac
     3421 cgtctgggcg ttcacaaata cgaagtcatg ggatgggatt acaaattgaa agaagtgaag
     3481 gaaaatactc cggaacaact ggaacgggca gaaaaactgt tcaaagagta tttcacgaat
     3541 gtgataacca actaaaacag aaaagcaagg ctgtccggat atgattccag acagccttgc
     3601 ttttcattta taggacaaga atcctattct ttcaagttat aggtaatctg ctgtacatca
     3661 tcactactgc cacccaccat aaagacgaaa tcaccaggtt ctaccatata attcatctca
     3721 ttgtcataga atcccagtgc atcgtcagtt atctgaaaag ctacagtttt gctttcaccc
     3781 gcattcagat gaattttcct gaagcccttc aactccttta tcgggcgcgt aacagacgct
     3841 accttatcct gcacatacaa ctggactact tcctctcctg cacacttacc ggtattagtc
     3901 acttttaccg acacctccgt atggacgcct gcatccttgc ccggcagcag tttcaaatca
     3961 gaatatccaa aatccgtata cgaaagccca tatccgaaag gataggcagg ctcagtggaa
     4021 gcatcgatat aggatgcctt ataagtctgt tgcaatgcgg taggacgacc ggtactctta
     4081 ttctggtaat aaatgggaat ctgccctacg tgtgcaggaa atgtcatggg caatttagcc
     4141 gaaggattat actctcccca caacacattg catatggcat tgccggcctc actccccagc
     4201 caccaagtac acagtaccgc atcagcatgt tcccttatct cattgaagat taccggacgc
     4261 ccacacatga caagagcaat gacaggtttt ccagtctttg ccagttccga caccagcttc
     4321 tgctgcccct cgggtatggc tatattcact ttggaagcct tctcgccact ctgaccgaat
     4381 ctctcgccta ccgccacaac aatgacatcc gaagcctcag cagcctgcaa tgcttcctgc
     4441 agtccggtca attgattgct cttcatatca tagccttcag catagttcac ctccagtcct
     4501 ttccggacca gtgcatcata caaggtcacc actttgctcc ggtccgtagt ggcggcccaa
     4561 gcgcctgcca tatcgtattg ggattttgag agtcctccta tcaaggctat cttcctcgtc
     4621 tgtgccgtca acggaagcac agattcccta ttctccagca atacaacgga acgctcggcc
     4681 atacggagcg aagcattacg gacttcgggc gcattgagta ttctggccgc tctttccgga
     4741 ttacagtagc gaaaagggtc ctcaaacaat cccaactcat atttcttctc caatatacgc
     4801 cgtactgcat catcaagcac actctccttt actttccctt cttgaacaag ctccttcaga
     4861 taagtcaagt agcaggtact taccatctcc atgtccagac cagcattcag cgctttggct
     4921 gcggcatctt tcttgtcaac agcataacgg tggttcacca attcctctac cgaattataa
     4981 tcggacacaa caaatccctt aaatccccac tgctctctca atagccggtt gagcaagaaa
     5041 tcattggcag tacaaggaat attattaaaa tcattgaaag cactcatcac ggtagctgct
     5101 cctaccttta tggccgcctt atagggaggc atataaaaat tggcaaagtg gcccattgac
     5161 atatccacac tattatactc ctttcctgct atcggtgcac catatccggc aaaatgtttc
     5221 atacatgcca tgacagtcgt cgtgtctgcc aaatcgtctc cttgcaagcc tctgacacgc
     5281 gcctccgcta cccgagaacc ataataaggg tcctcaccgg ccccttccat gacgcgtccc
     5341 caacgtgcgt cccacgaaat atccaccatc ggggcaaaaa cccagttcaa accatcggcg
     5401 gcactttcca aagcattgta gcgtgcagct tcctcaatca tggccaaatc gaaacttgca
     5461 gcctcggcaa gcggaagcgg aaatccggta cgatagccgt ggataacatc cagtccgaaa
     5521 attaaaggaa tgcgcaaacg cgattgcaaa gcaagctctt gataacggcg tatatccttt
     5581 actcctatca cattcagcat agagcctatt ctgccttgcc ggacatactc ttctttagga
     5641 atatttccac cgtcgggtcc ggtaaaattc cctcggtagg tgcattgtgc aagctggcct
     5701 atcttttcat ccagcgtcat ctgcttcatc aagcttgaga tgaatttctc cttctccgta
     5761 gtttgtgccg aggtaaaggt gttaccaagt c
//
