LOCUS       c00395_gnlPro..         3370 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|HKOPHDEH_395.
ACCESSION   c00395_gnlPro..
VERSION     c00395_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 07:00:57
            Original ID  :: gnl|Prokka|HKOPHDEH_395
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3370
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3370
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:2461]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2..2461
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..3370
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3370
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             2..1672
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 152.2; E-value:
                     7.4e-47)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg395_1"
                     /sec_met_domain="FAD_binding_2 (E-value: 3.6e-48, bitscore:
                     152.2, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 1.8e-38,
                     bitscore: 119.2, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="SVYRLFYDTVKGGDYRAREANVYRLAEVSNNIIDQCVAQGVPFAR
                     EYGGTLDNRSFGGAQVSRTFYAKGQTGQQLLLGAYSALSRQVNVGTVKLYTRYEMEDVV
                     LIDGRARGIIAKNLVTGKLERFAAHAVVIATGGYGNAYFLSTNAMACNCSAAMACYRKG
                     AWFANPAYVQIHPTCIPVHGDKQSKLTLMSESLRNDGRIWVPKKLEDAKKLQEGTLQGK
                     DIPEEDRDYYLERRYPAFGNLVPRDVASRAAKERCDKGFGVNNTGLAVFLDFSEAINRL
                     GKDVVAQRYGNLFDMYEEITDVSPYENPMMIYPAIHYTMGGIWVDYELMTSIKGLFAIG
                     ECNFSDHGANRLGASALMQGLADGYFVLPYTIQNYLADQITVPRFSTDLPEFAAAEKAV
                     QEKIDWMMNIKGKKSVDSIHKELGHIMWEYVGMGRTAEGLKEGLKKLKEVRKEFEKELF
                     IPGDKEGMNVELDKAIRLYDFITMGELVAYDALNRNESCGGHFREEYQTEEGEAKRDDE
                     NYFYVACWEYQGSDEKEPVLLKEPLVYEAIKVQTRNYKS"
     CDS             1706..2461
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer4_7"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg395_2"
                     /sec_met_domain="Fer4_7 (E-value: 2.6e-13, bitscore: 38.3,
                     seeds: 208, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_3 (E-value: 8.2e-27, bitscore: 81.2,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 9.7e-08, bitscore: 20.2,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKNISFTLKVWRQKGPKAKGAFETYQMKDIPGDTSFLEMLDILN
                     EQLISEGKEPVVFDHDCREGICGMCSLYINGHPHGPATGATTCQIYMRRFNDGDTITVE
                     PWRSAGFPVIKDLMVDRTAYDKIMQAGGYVSVRTGAPQDANAILIPKPIADEAMDAASC
                     IGCGACVAACKNGSAMLFVSAKVSQLNLLPQGKPEALRRAKAMLSKMDELGFGNCTNTR
                     ACEAECPKNVSISNIARLNRDFIIAKLKD"
     CDS             complement(2600..3370)
                     /locus_tag="ctg395_3"
                     /transl_table=11
                     /translation="GFHLPARKCEENIIIFLLKGEVLVNSKEYAGTVLHAGEFILQAIG
                     SKYEILAMTDVECVCYRFSKPEFFCEDRYNHIMKEVTPPLIFYPLTITPELQLFLESSK
                     AYLSEEKICREMLCFKRKELAFILGNYYSDYELSMLIHPLAQYTNSFHYFVLQNHAKVK
                     TVEELAQLGGYTVATFRRIFNSVFHQPVYEWMMERRKESVVYELRYTDASISEICYKYG
                     FESLPHFSNFCKKNFGASPRSLRAGNLSEVNGMS"
ORIGIN
        1 ctctgtatac cgtctgttct acgatacagt aaagggtggc gactaccgtg cccgcgaagc
       61 caacgtttac cgtctggctg aagtatccaa caatattatc gaccaatgcg ttgcacaagg
      121 tgttcctttc gcccgcgaat acggcggtac actggacaac cgttctttcg gtggtgctca
      181 ggtatcccgt actttctatg ccaagggtca gaccggacag cagctgttgc tgggtgcata
      241 ctctgcattg agccgccagg tgaacgtagg cactgtgaaa ctgtataccc gctacgaaat
      301 ggaagacgtt gtcctcattg acggacgtgc ccgcggtatc attgcaaaga acctcgttac
      361 cggtaaattg gaacgttttg ccgcccacgc tgtggtaatc gctaccggtg gttacggcaa
      421 tgcctacttc ctttctacca atgcaatggc atgtaactgc tctgcagcaa tggcttgcta
      481 ccgtaaaggt gcctggtttg ccaaccctgc ttacgtacag attcacccca cttgtatccc
      541 ggttcacggt gacaagcagt ctaagctgac tttgatgtcc gaatctctgc gtaacgatgg
      601 tcgtatctgg gttccgaaga agctggaaga tgccaagaaa ctgcaggaag gtacactgca
      661 aggaaaagat attcccgagg aagaccgcga ctactacctg gaacgccgtt atccggcatt
      721 cggtaacctc gttccgcgtg acgttgccag ccgcgctgcc aaagaacgct gcgacaaggg
      781 cttcggcgtg aacaacaccg gtcttgccgt attccttgac ttctccgaag ctatcaaccg
      841 tctgggtaaa gatgtcgttg cacaacgtta cggcaacctc ttcgatatgt atgaagaaat
      901 cactgatgtc agcccatacg aaaacccgat gatgatttat ccggctatcc actataccat
      961 gggtggtatc tgggttgact acgaactgat gacctccatc aagggactgt tcgccatcgg
     1021 tgaatgtaac ttctccgacc acggtgcaaa ccgcctcggt gcttccgccc tgatgcaagg
     1081 tctggccgat ggttacttcg tattgcctta cactatccag aactatctgg ccgaccagat
     1141 tacagttccc cgtttctcta ccgacctgcc cgaatttgca gctgcagaaa aggccgttca
     1201 ggagaagatt gactggatga tgaacatcaa gggtaagaag tctgtagact ctatccacaa
     1261 ggaactgggc cacattatgt gggaatatgt aggtatggga cgtacggccg aaggcttgaa
     1321 ggaaggtctg aagaagctga aagaagtccg caaggagttc gagaaagaac tgttcatccc
     1381 gggcgacaag gaaggtatga atgtagaact cgacaaggct atccgtctgt acgacttcat
     1441 cactatgggc gagctggttg cttacgatgc actgaaccgt aacgaaagct gcggcggtca
     1501 cttccgtgaa gaatatcaga ctgaagaagg cgaagccaag cgtgacgatg aaaactactt
     1561 ctacgtggct tgctgggaat atcagggttc tgacgaaaag gaaccggtat tgctgaaaga
     1621 gccgctggtt tacgaagcaa tcaaggtaca gactcgtaac tacaaaagct aatcgggaag
     1681 ttgaacattt aaagaattaa agaaaatgga taaaaatata tcatttacgc tcaaggtatg
     1741 gcgccagaag ggtccgaaag caaaaggcgc ttttgaaaca taccaaatga aagatatccc
     1801 gggcgatact tcattcctcg aaatgctgga tatcttgaac gaacagctga tttctgaagg
     1861 taaagaaccg gttgtattcg accacgactg ccgcgaaggt atctgcggta tgtgttcgct
     1921 ctacatcaac ggacaccccc acggtcctgc aaccggcgct acgacttgcc agatttatat
     1981 gcgtcgtttc aacgacggcg ataccatcac tgttgaaccg tggcgctctg ccggtttccc
     2041 ggttatcaag gacttgatgg tcgaccgtac ggcctacgac aagattatgc aggcaggcgg
     2101 ctacgtcagc gtacgtaccg gtgctcccca ggatgccaac gctatcctga ttccgaagcc
     2161 catcgctgac gaggctatgg atgctgccag ctgtatcggt tgtggtgctt gtgtggctgc
     2221 ttgtaagaac ggttctgcta tgctgttcgt ttctgccaag gtgagccagc tgaacctgtt
     2281 gccccaaggc aaaccggaag cattgcgccg tgccaaggct atgttgagca agatggacga
     2341 actcggtttc ggtaactgta caaacactcg tgcgtgcgaa gctgaatgcc cgaagaatgt
     2401 ttccatcagc aacattgctc gcctgaaccg cgactttatc attgcgaaac tgaaagacta
     2461 atccggtctt tctgcaaata atataggatg cgcttcggat aaaccttttg agcaagctca
     2521 gatttatcac tcagctttca ctatatttga ttaatatcac aagagactcc tctgccggga
     2581 aaccgccgga ggagtttttt tatgacatgc cgtttacttc agataaattg ccggcacgca
     2641 agctacgggg agaagccccg aaattctttt tgcagaaatt ggaaaaatga ggcagcgatt
     2701 caaagccata tttatagcaa atctctgata tagaagcatc cgtataacga agttcataaa
     2761 ccacgctttc cttcctccgt tccatcatcc actcgtacac cggttgatga aaaacagaat
     2821 tgaatatacg ccggaaagta gctactgtat atcctcccaa ctgggcaagt tcctctaccg
     2881 tcttgacttt ggcatgattc tgcaagacaa agtagtggaa ggaattagta tactgggcaa
     2941 gaggatgtat cagcatggaa agctcatagt cggagtagta atttccaaga atgaaagcaa
     3001 gttctttccg tttgaaacaa agcatttccc ggcaaatctt ctcctcggac aaataggcct
     3061 tggaactttc cagaaagagc tgcaactcgg gagtaatggt caaaggatag aatatcaatg
     3121 gaggggtaac ctctttcata atatggttat accggtcctc acagaagaat tcaggcttac
     3181 tgaaccggta acaaacgcat tccacatccg tcatggcaag tatttcgtac ttggaaccaa
     3241 tagcttgaag gatgaactca cctgcatgca acacagttcc cgcatactcc ttactgttga
     3301 caagcacttc tcctttcagc aggaaaatta taatattttc ctcacacttt cgcgccggca
     3361 gatggaatcc
//
