LOCUS       c00176_gnlPro..         4312 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|JAOAAGEL_176.
ACCESSION   c00176_gnlPro..
VERSION     c00176_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 07:41:17
            Original ID  :: gnl|Prokka|JAOAAGEL_176
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4312
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4312
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1815:4312]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1816..4312
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4312
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4312
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..1747
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fn3-like"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3_C"
                     /gene_functions="other (smcogs) SMCOG1041:Fibronectin type
                     III-like domain (Score: 87.5; E-value: 1.6e-27)"
                     /gene_kind="other"
                     /locus_tag="ctg176_1"
                     /sec_met_domain="Fn3-like (E-value: 7.8e-29, bitscore:
                     87.5, seeds: 729, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3 (E-value: 1.8e-52, bitscore:
                     166.3, seeds: 11, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3_C (E-value: 1.2e-53,
                     bitscore: 169.7, seeds: 116, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVDISWDARWGRVMEGAGEDPYYGSRVAEARVRGLQGDDLADTTT
                     VMACMKHFAGYGAPIAGKEYNSVDMSMGHFANFYMPPYKAAIKVGAATVMSAFNDFNNI
                     PCTANDFLLNRLLREQWGFKGFVVSDYNSVEELVNHRYAVDKKDAAAKALNAGLDMEMV
                     STCYLTYLKELVQEGKVKESVLDDAVRRILEKKYELGLFEDPFRYCNPERAARILNAPE
                     VRNASLRMAERSVVLLENRESVLPLTAQTRKIALIGGLSKSQYDMAGAWAATTDRSKVV
                     TLYDALVRKGLEVNYAEGYDMKSNQLTGLQEALQAAEASDVIVVAVGERFGQSGEKASK
                     VNIAIPEGQQKLVSELAKTGKPVIALVMCGRPVIFNEIREHADAVLCTWWLGSEAGNAI
                     CNVLWGEYNPSAKLPMTFPAHVGQIPIYYQNKSTGRPTALQQTYKASYIDASTEPAYPF
                     GYGLSYTDFGYSDLKLLPGKDAGVHTEVSVKVTNTGKCAGEEVVQLYVQDKVASVTRPI
                     KELKGFRKIHLNAGESKTVAFQITDDALGFYDNEMNYMVEPGDFVFMVGGSSDDVQQIT
                     YNLKE"
     CDS             complement(1816..2541)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 75.5;
                     E-value: 1.7e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg176_2"
                     /sec_met_domain="Radical_SAM (E-value: 5.5e-32, bitscore:
                     99.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCPFRCLYCANPDTIDVKGG
                     TPTTPEEILQMAISQKAFFGKKGGITFSGGEPTLQAEALIPLFKELKANGIHICLDSNG
                     GIWNEKVEELFSLTDLVLLDIKQFNPERHRTLTGRSNEQTLRTAAWLEEHEHPFWLRYV
                     LVPGYSDSEEDIRLLGESLGKYRQIQRVEILPYHRLGVHKYEVMGWDYKLKEVKENTPE
                     QLERAEKLFKEYFTNVITN"
     CDS             complement(2543..4312)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg176_3"
                     /sec_met_domain="Gly_radical (E-value: 1.2e-47, bitscore:
                     148.7, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 8.5e-280, bitscore:
                     916.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="SLGFLTGLPDNYARGRIIGDYRRLALYGIDRLIEAKQEDLRNLTG
                     PMTEARIRLREEVAEQIKALKDIKVMGEYYGLDLSHPATSAQEAVQWVYMAYLAAIKEQ
                     DGAAMSLGNVSSFLDIFIEYDLAHGKINETFAQELIDQFVIKLRMVRHLRMQSYNDIFA
                     GDPTWVTEAIGGRFNDGRVKVTKTSFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKDFC
                     AKVSVDTSSIQYENDNLMREVRNCDDYGIACCVSYQAIGKQIQFFGARANLAKALLLAI
                     NGGRCENTGTVMVKGIPVLTGETLKFEEVMANYKKVLTEIARVYNEAMNIIHYMHDKYY
                     YEKAQMAFIDTNPRINLAYGVAGLSIAIDSLSAIKYAKVTARRNEIGLTEGFDTEGSFP
                     CFGNNDDRVDHLGVDLVYYFSEELKKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGR
                     AKGVAFAPGANPMHGRDKNGAIASLSSVAKLRYRDSQDGISNTFSIVPKSLGPTPEERV
                     ENLVTMMDGYFTKGAHHLNVNVLNREMLEDAMEHPEKYPQLTIRVSGYAVNFVKLSREH
                     QLEVISRSFHERM"
ORIGIN
        1 gatggtggat atttcgtggg acgcacgttg gggacgcgtc atggaagggg ccggtgagga
       61 cccttattat ggttctcggg tagcggaggc gcgtgtcaga ggcttgcaag gagacgattt
      121 ggcagacacg acgactgtca tggcatgtat gaaacatttt gccggatatg gtgcaccgat
      181 agcaggaaag gagtataata gtgtggatat gtcaatgggc cactttgcca atttttatat
      241 gcctccctat aaggcggcca taaaggtagg agcagctacc gtgatgagtg ctttcaatga
      301 ttttaataat attccttgta ctgccaatga tttcttgctc aaccggctat tgagagagca
      361 gtggggattt aagggatttg ttgtgtccga ttataattcg gtagaggaat tggtgaacca
      421 ccgttatgct gttgacaaga aagatgccgc agccaaagcg ctgaatgctg gtctggacat
      481 ggagatggta agtacctgct acttgactta tctgaaggag cttgttcaag aagggaaagt
      541 aaaggagagt gtgcttgatg atgcagtacg gcgtatattg gagaagaaat atgagttggg
      601 attgtttgag gacccttttc gctactgtaa tccggaaaga gcggccagaa tactcaatgc
      661 gcccgaagtc cgtaatgctt cgctccgtat ggccgagcgt tccgttgtat tgctggagaa
      721 tagggaatct gtgcttccgt tgacggcaca gacgaggaag atagccttga taggaggact
      781 ctcaaaatcc caatacgata tggcaggcgc ttgggccgcc actacggacc ggagcaaagt
      841 ggtgaccttg tatgatgcac tggtccggaa aggactggag gtgaactatg ctgaaggcta
      901 tgatatgaag agcaatcaat tgaccggact gcaggaagca ttgcaggctg ctgaggcttc
      961 ggatgtcatt gttgtggcgg taggcgagag attcggtcag agtggcgaga aggcttccaa
     1021 agtgaatata gccatacccg aggggcagca gaagctggtg tcggaactgg caaagactgg
     1081 aaaacctgtc attgctcttg tcatgtgtgg gcgtccggta atcttcaatg agataaggga
     1141 acatgctgat gcggtactgt gtacttggtg gctggggagt gaggccggca atgccatatg
     1201 caatgtgttg tggggagagt ataatccttc ggctaaattg cccatgacat ttcctgcaca
     1261 cgtagggcag attcccattt attaccagaa taagagtacc ggtcgtccta ccgcattgca
     1321 acagacttat aaggcatcct atatcgatgc ttccactgag cctgcctatc ctttcggata
     1381 tgggctttcg tatacggatt ttggatattc tgatttgaaa ctgctgccgg gcaaggatgc
     1441 aggcgtccat acggaggtgt cggtaaaagt gactaatacc ggtaagtgtg caggagagga
     1501 agtagtccag ttgtatgtgc aggataaggt agcgtctgtt acgcgcccga taaaggagtt
     1561 gaagggcttc aggaaaattc atctgaatgc gggtgaaagc aaaactgtag cttttcagat
     1621 aactgacgat gcactgggat tctatgacaa tgagatgaat tatatggtag aacctggtga
     1681 tttcgtcttt atggtgggtg gcagtagtga tgatgtacag cagattacct ataacttgaa
     1741 agaataggat tcttgtccta taaatgaaaa gcaaggctgt ctggaatcat atccggacag
     1801 ccttgctttt ctgttttagt tggttatcac attcgtgaaa tactctttga acagtttttc
     1861 tgcccgttcc agttgttccg gagtattttc cttcacttct ttcaatttgt aatcccatcc
     1921 catgacttcg tatttgtgaa cgcccagacg gtggtaagga agtatttcca cccgttgtat
     1981 ctgccggtat ttgccgagac tttcacccag caggcggatg tcttcttccg aatcgctgta
     2041 accgggaacc agtacataac gcagccagaa ggggtgttca tgttcttcga gccaggcagc
     2101 ggtgcggagt gtctgttcgt tgcttcgtcc cgtcagcgta cggtggcgtt cgggattgaa
     2161 ttgcttgatg tccagcagca cgaggtcagt caggctaaag agctcttcca ccttttcatt
     2221 ccagatgcct ccgttactgt ccagacagat atggatgccg ttcgccttca gctccttgaa
     2281 aagaggaatc agcgcttctg cttgcagggt aggctcaccg ccggagaaag taatacctcc
     2341 tttcttcccg aagaatgcct tttggctgat agccatttgc agaatctctt ccggagtggt
     2401 gggagtgcct ccttttacat caatcgtatc cggattggca caatacagac aacggaacgg
     2461 acatccttgc aggaaaacga cgagccggag acccggcccg tcgaatgttc ccatactttc
     2521 gtaggagtgt acatttatca tattacattc tttcgtggaa tgaacggctg atgacttcca
     2581 actgatgctc gcggctcaac ttcacgaagt tcacggcata gccggaaacg cggatggtga
     2641 gttgcggata cttttcagga tgttccatgg catcttccag catttcgcgg ttcagtacgt
     2701 ttacgttcaa atggtgggca cctttggtga agtagccgtc catcatggtt accaggttct
     2761 ctacacgttc ttccggtgtc gggcccagtg atttaggaac aatggagaac gtattgctga
     2821 tgccgtcctg cgagtcgcgg tagcgcaatt tggctacgga gctcaaggaa gcgatggcac
     2881 cgttcttgtc acgtccgtgc attgggttgg cacccggagc aaaggcaaca cctttggcgc
     2941 gtccgtccgg agtggcaccg gtcttcttgc cgtacatcac gttggaggtg atagtcagca
     3001 aggaaagggt gggacgggca ttcttgtaga caggcagctt tttcagttct tcgctgaaat
     3061 agtaaacgag gtctactccc aggtggtcca cacggtcgtc attattgccg aagcagggga
     3121 atgagccttc agtgtcgaaa ccttcggtca ggccgatttc gttgcggcgt gcagtgactt
     3181 tggcatattt gatggcggac agtgagtcta tggcgatgga aaggccggca acaccgtatg
     3241 ccagattgat gcgtgggttg gtatcgatga atgccatctg ggccttctca taataatact
     3301 tatcatgcat gtagtggatg atattcatgg cctcgttgta gacacgggct atttcagtca
     3361 gtaccttctt gtagttggcc ataacttcct caaacttcag tgtctcgccg gtcaatacgg
     3421 gaataccttt caccatgaca gttccggtgt tttcgcaacg gccaccgttg atggcaagca
     3481 gcagggcctt ggcaaggttg gcacgtgcgc cgaagaactg gatttgcttg ccgatggctt
     3541 ggtaggatac acagcaggca atgccgtaat cgtcgcagtt gcgcacttcg cgcatcaggt
     3601 tgtcgttttc gtactggatg gagctggtat ctacggaaac tttggcgcag aaatccttga
     3661 agccttccgg cagttcggga ctccacagta cggtcaagtt tggttccgga gaaggaccga
     3721 ggttgtatag cgtctgcaag aagcggaaag aagtcttggt gactttcacg cgtccgtcat
     3781 tgaagcgtcc tccgatggct tcggtcaccc aagtcgggtc accggcaaag atatcattgt
     3841 acgactgcat acgcagatgg cgtaccatgc gcagcttgat gacgaactgg tctatcagtt
     3901 cttgcgcaaa cgtctcattg atttttccgt gagccagatc gtattcaatg aatatatcga
     3961 ggaacgaaga tacgttaccc aatgacatgg cagcaccgtc ttgctctttg atggcagcaa
     4021 gatatgccat gtatacccat tgcacggcct cttgagctga tgtagccgga tggctgaggt
     4081 cgagtccgta atattcgccc ataaccttga tgtccttcaa agctttgatt tgttccgcca
     4141 cttcttcgcg tagacggatg cgtgcttcgg tcatcgggcc ggtgaggttg cgcaagtcct
     4201 cctgcttggc ttcaatcaga cggtcgatgc cgtagagagc caatcggcgg tagtcgccga
     4261 tgatacgtcc gcgggcatag ttgtcgggca atccggtaag gaatcccagt ga
//
