LOCUS       c00250_gnlPro..         5688 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|MKDPDFBL_250.
ACCESSION   c00250_gnlPro..
VERSION     c00250_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 07:48:59
            Original ID  :: gnl|Prokka|MKDPDFBL_250
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5688
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5688
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:3265]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..3265
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..5688
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5688
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             complement(3..185)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg250_1"
                     /sec_met_domain="OAD_beta (E-value: 7.7e-17, bitscore:
                     50.3, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGIFSLSWQMVGMWGIAILLLYLGVAKQYEPLLMVPIAFGALIAN
                     IPDNGMLITQQIGRAH"
     CDS             complement(303..704)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 59.8; E-value:
                     5.7e-19)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg250_2"
                     /sec_met_domain="Biotin_lipoyl (E-value: 7.4e-20, bitscore:
                     59.8, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKLRITVDGKVFDVSVELLDQVSSTTAAPAPAPVAAPAPAASAP
                     VAAPAPAPAPAPAPAAAGAGDVPSPLAGKVVSLDVAAGASVKAGDQILTLEAMKMNTII
                     YAPASGTLTAFCVNPGDTVQEGQALAKIG"
     CDS             complement(779..1225)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg250_3"
                     /sec_met_domain="OAD_gamma (E-value: 1.7e-11, bitscore:
                     33.8, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLLTTLAFAQGIANVMNNLNYQIVGIMVVMMCLGGLAVILTISGS
                     IAAAIQNRAKAKTAAPVAAPAAAPAAAGKPGMTPEMLAILSAAVDSAMTELTPEMVAVI
                     SAAVDAGLDGMSHRIVEIKQSPGSGYAAAGRAEIFASHRIRPSR"
     CDS             complement(1246..2811)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Vparvula"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg250_4"
                     /sec_met_domain="CT_Vparvula (E-value: 7.3e-217, bitscore:
                     709.8, seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAIDPKLIDDLNSRRKKVILSGGQEKIDKRHEKGEMTARDRMGYL
                     FEEGTFSEIGMHVRHNCHNFGMGKKEIPGDGVVSGFGLVDGRPVACAASDFLAQGGSLG
                     YMHAMKIADAQKYALKAGIPMVTVNDSGGARLQEGVAALSGYAQVFYNNVLASGVVPQI
                     SMILGPCAGGAAYSPALTDFIIMRNSGNAGMYITGPKVIEQVTYEKCTMDDIGSAAIHA
                     TVSGNVHFVADSDAHAMDILKRLLSFLPSNNTEEPPHKLDTPLDLSADEGMSDLIPGDN
                     RTPLDVQPIISRLVDNGDFLEVHKDFAKNVVVGFGRICGVVVGIIANQPNVKAGCLDID
                     SSDKAARFIRFCNAFNIPLVNLVDVPGFLPGKNQERGGIIRHGAKLIFAYSQATVPKVT
                     LIMRKAYGGAYIAMCCKDLGADAVFAWPGAEIAVMGAEGAVPVLYGRELKAVEDPAEKA
                     KRQGELLEEYREAFYNPYVAAGMGQITEVINPEETRAKIAFALRTLLNKKEVRPAKKHG
                     NIPL"
     CDS             complement(2843..3265)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Glyoxalase_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg250_5"
                     /sec_met_domain="Glyoxalase_4 (E-value: 4.1e-26, bitscore:
                     80.6, seeds: 51, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIQQIDHIGIAVKSLDATVPYYRDALGLGEPHIEEVPTQKVRVAM
                     FDVAGVHIELLEPTSPESAIAKAIEKKGEGIHHIAFKTNDIAGNISQAKEAGLTVLNDP
                     PVPGAHNTQVTFLHPKCTFGVLTEFCQHPGGCECGK"
     CDS             3432..4970
                     /locus_tag="ctg250_6"
                     /transl_table=11
                     /translation="MFKSGLAVLFLLASWFSAACAYELLPAHVAVVYNGKSELSRRMAR
                     EYARVRGVPEGNLVSLDCPMTGEISRKEYEETIRVPLLETARKQRWWTPSGIASSPLMN
                     RKIFVLALMADLPMKIRHETPAPLPGKGVNQMQTDRAAVDSELALLAVGGYERKSWQVN
                     PYFNKREDFVGSGLPSFLVCRLDGLTPDTCMRLVTEPANVEKKGLWGWAVVDRGGPYAQ
                     GDRWMDDVFERVREAGIPAYLDDWPQTLPEKFPLSRDTALYCGWYAGNANGPFSDPSFR
                     FRPGAIAMHLHSFSAADFKVPGRGWSSALLEKGAAVTVGNVYEPFLGACHRFDIFVDRL
                     LDGYTVAEASWMSMPVLSWQGVVFGDPLYRPYARMKDMDVEPTEEDRYFQGWWASSVQF
                     GDRWKERSARLMESARKAPFSCLYEALALECLYRKEPARAGELLSSALDGAADARTRAR
                     LLLEILMAERARGGNKAFLQRADGIRGLMSSSAFLPALEEWLARVAPPPAPPKK"
     CDS             5080..5427
                     /locus_tag="ctg250_7"
                     /transl_table=11
                     /translation="MNIINKIEQEQLKADVTPFNVGDTIKVHTRVIEGGKERIQIFQGI
                     VIAKRGSGINEAFTVRKISYGEGVERVFPLHTPRIAKIEVVNRGKVRRAKLHYLRGRIG
                     KDAMIVKSANR"
     CDS             complement(5543..5686)
                     /locus_tag="ctg250_8"
                     /transl_table=11
                     /translation="HSPEAIQARTEENPKNVFAPDRKQPPLSTFPTPEKRSHPHMAPGE
                     IS"
ORIGIN
        1 acgtgtgctc ttccgatctg ctgggtgatg agcattccgt tgtccggaat gttggcgatg
       61 agggcgccga aagcaatggg aaccatcagg agaggttcgt attgcttggc tacacccaga
      121 tacaggagca ggatggcaat gccccacatc cctaccattt gccatgatag ggagaagatg
      181 cccatcccct gtaaaaaggt gattaatgaa tcaaaaagac tcatacggaa tgttattgta
      241 gcggtttgtt atctgagtcg gagccaggaa ctgttggagg ttccgggcgg ttgccgccgg
      301 gattaaccaa ttttggccag agcctgtcct tcctgaaccg tatcacccgg gtttacgcag
      361 aaagcggtga gcgtgccgga ggcgggagcg tagatgatgg tattcatctt catggcttcc
      421 agcgtcagaa tctggtcgcc ggccttcacg gaggcgccag cggccacgtc cagggaaaca
      481 accttgccgg caagcgggct gggcacgtct ccggcgccgg cggcggccgg agcgggcgca
      541 ggtgcgggag ccggagccgg ggcggccacg ggtgcggatg cggcaggagc gggagcggcc
      601 acaggtgcgg gggccggggc tgcggtagtg gaggatacct ggtccaggag ttctacggat
      661 acgtcaaata ccttgccgtc tacggtgatg cgaagtttct tcatgataat gtatggtatg
      721 gagtattaag ttaaatggtt cccgccgggg cgggggtaag ggtgaataat gctgtagttc
      781 aacgggaagg ccgaatgcgg tgggaggcga agatttccgc cctgcctgcg gccgcatagc
      841 cggagcccgg ggattgcttg atttccacaa tgcggtggct catgccgtcc aggcccgcgt
      901 ccacggcggc ggagatgacg gccaccattt ccggcgtgag ctccgtcatg gcggagtcca
      961 ccgctgcgga gaggatggcg agcatttccg gcgtcatgcc cggtttgccc gccgcagcgg
     1021 gggcggcggc aggcgcggcc acgggagcgg cggttttggc tttagccctg ttctgaatgg
     1081 cggcggcgat ggagccgctg atggtcagaa tgacggccag gccacccagg cacatcatga
     1141 ccaccataat gccgacaatc tgatagttca gattgttcat gacgttggcg atgccctgtg
     1201 caaaggcgag tgttgtgagt aacatatgat ccgttgagat ggatgttaga gcggaatgtt
     1261 gccgtgtttc ttggccgggc gcacttcctt cttgttcagc agggtgcgca gggcgaaggc
     1321 gattttggcg cgggtttctt ccggattgat gacttccgtg atctgcccca tgccggccgc
     1381 cacatacggg ttgtagaagg cttcccggta ttcttcaagg agttctccct ggcgtttggc
     1441 tttttccgcc gggtcttcca cagccttcaa ttcacggccg tagagcaccg gaacggcgcc
     1501 ttccgcgccc atgacggcga tttccgcgcc gggccaggcg aagacggcgt cagcgcccag
     1561 gtccttgcag cacatggcga tgtaggcgcc gccgtaggct ttccgcatga tcagggtgac
     1621 tttgggcacc gtggcctggg aataggcgaa gatgagtttg gcgccgtggc ggataatgcc
     1681 gccccgttcc tgattcttgc cgggcaggaa gccgggcacg tccaccaggt tcaccaacgg
     1741 aatattgaac gcgttgcaga agcggatgaa gcgcgcggcc ttgtcggagg agtcgatatc
     1801 caggcagccg gctttcacgt tgggctggtt ggcgatgatg ccgactacta cgccgcagat
     1861 gcggccgaag ccgacgacga catttttggc gaagtcctta tgcacttcca ggaagtcccc
     1921 gttgtccacc aggcggctga tgatgggctg gacgtccagc ggcgtgcggt tgtcgccggg
     1981 aatcaggtcg ctcatgcctt cgtctgcgct caggtcaagc ggggtatcca gtttgtgggg
     2041 cggttcttcc gtgttgttgg aaggaaggaa ggaaagcagc ctcttcagga tgtccatggc
     2101 gtgcgcgtcg ctgtccgcta cgaaatggac gttgccggac acggtggcgt gaatggcggc
     2161 ggagccgatg tcgtccatcg tgcatttttc atacgtgacc tgttcaatca ccttggggcc
     2221 ggtgatgtac atgccggcgt tgccggaatt gcgcatgatg atgaagtccg tcagggcggg
     2281 ggagtaggcc gcgccgcccg cgcagggacc cagaatcatg gagatttgcg gaaccacgcc
     2341 ggaggcaagc acattgttgt agaacacctg ggcatagccg gaaagggccg ccacgccttc
     2401 ctggaggcgc gcgccgccgg agtcgttcac ggtcaccatc gggatgccgg ctttcagggc
     2461 gtacttctgg gcatccgcaa ttttcatggc gtgcatgtag ccgagggacc cgccctgggc
     2521 caggaagtcg gaggccgcgc aggccaccgg acggccgtcc accaggccga agccggaaac
     2581 gacgccgtcg ccggggattt ccttcttgcc catgccgaag ttgtggcagt tgtggcgcac
     2641 atgcatgccg atttccgaga aggtgccttc ctcaaagagg taccccatgc ggtcgcgggc
     2701 tgtcatttcg cccttttcat gtctcttgtc gatcttttcc tgaccgccgc tgaggattac
     2761 cttcttgcgg cggctgttca gatcgtcaat caatttggga tcaatagcca ttggtatagt
     2821 ggtatggtga atagggagaa tgttacttgc cgcattcgca gccgccgggg tgctggcaga
     2881 attcggtcag gacgccgaag gtgcatttgg gatgcaggaa ggtgacctgc gtgttgtggg
     2941 cgccggggac gggcgggtcg ttcagcacgg taaggcccgc ttccttggcc tgggagatat
     3001 ttccggcgat gtcattggtc ttgaaagcga tgtggtggat gccttcccct tttttctcaa
     3061 tagccttggc gatagcgctt tccggagagg tgggttcgag cagttcgatg tggacgccgg
     3121 caacgtcaaa catggcgacg cggacctttt gagtggggac ttcctcaatg tggggctcgc
     3181 cgagcccgag cgcatcgcgg taatagggaa cggtggcgtc aagactcttg acggcgatgc
     3241 cgatatggtc aatttgctga atcatatgcg tgtgatggtt ataggggaat gcagggggga
     3301 ggcctgcaat acatcaagag ttattaaagc ggattttttt aagatgtgaa gcgaaaagcg
     3361 gaaaaatggc gaattcgtgg ccggcctttt cacctgcttg attgccccgg aagatgaggt
     3421 atggtagggg catgttcaag agtggattag ccgtgttatt tctgctggcg tcatggtttt
     3481 ccgccgcgtg tgcctatgag ctgctgcccg ctcatgtggc cgtggtttat aacggaaaat
     3541 ccgagctgag ccgccgcatg gccagggaat atgcgcgggt gcgcggcgtt ccggaaggga
     3601 atctcgtttc cctggattgc cccatgaccg gcgagatttc gcggaaggag tatgaggaga
     3661 cgatccgcgt cccgctgctg gagacggcgc ggaaacaacg gtggtggacg ccttccggca
     3721 ttgcgtcctc ccccttgatg aaccggaaga tttttgtgct ggcgctgatg gcggacctgc
     3781 cgatgaagat acggcatgaa acgcccgctc cccttcccgg gaaaggcgtc aaccagatgc
     3841 agacggatcg cgctgcggtg gattccgaac tggctctgct ggcggtgggc ggttatgaaa
     3901 ggaagtcctg gcaggtgaat ccctatttta ataaaaggga ggattttgtg ggttccggcc
     3961 ttccttcgtt tctggtatgc cgcctggacg gactgacgcc cgatacctgc atgcggctgg
     4021 tgacggaacc ggcgaacgtg gagaaaaaag gtttatgggg gtgggccgtt gtggaccggg
     4081 gcggccccta tgcccagggg gaccggtgga tggacgacgt gttcgagcgc gtgagggagg
     4141 cgggcattcc cgcttatctg gatgactggc cccagaccct gccggaaaaa ttccccttga
     4201 gccgggatac ggctctttac tgcgggtggt atgcggggaa tgcgaacgga ccgttttcgg
     4261 atccttcgtt ccgcttccgg cccggggcga tagccatgca tctgcattcc ttcagcgccg
     4321 ccgatttcaa ggtccccggc agggggtgga gttccgccct gctggagaag ggggctgctg
     4381 tgacggtggg aaacgtgtat gagccgttcc tgggcgcctg ccaccgcttt gatatttttg
     4441 tggaccgcct cctggatggc tataccgtgg cggaggcttc ctggatgagc atgcctgtgc
     4501 tgtcctggca gggggtggtg ttcggagacc ccctgtaccg tccatacgcc cgcatgaagg
     4561 atatggacgt ggagccgacg gaagaagacc gttatttcca ggggtggtgg gcttcctccg
     4621 tgcagttcgg agaccgctgg aaggagcgtt ccgcgcgttt gatggagtcc gcccggaagg
     4681 cccctttttc ctgcctttat gaagctctgg cgctggaatg cctgtaccgg aaggaaccgg
     4741 cccgcgccgg agagctcctt tcctccgctc tggacggcgc ggcagacgcc cgcacccgcg
     4801 cgcgtctgct gctggaaatc ctgatggcgg aacgggcccg gggcggtaac aaggcctttc
     4861 tgcagcgtgc ggacggcatc cggggcctca tgtcttcctc cgcttttctt ccggcgctgg
     4921 aggaatggct tgccagggtg gctccgccgc cggccccgcc gaaaaaataa attaaggacg
     4981 tacggcaaat ttggattgcc ttttagggga ttatggctca tattctgcgt cccgtaagtt
     5041 ttccttttac tgatattcac cagcaataga taacacaaga tgaacattat caacaaaatc
     5101 gagcaagagc aactcaaggc ggatgtgacc ccatttaacg taggtgatac catcaaggtt
     5161 cacactcgtg ttattgaagg cggcaaggaa cgtatccaga ttttccaggg cattgtgatc
     5221 gccaagcgcg gttccggcat caatgaagct ttcaccgtcc gcaaaatttc ctacggtgaa
     5281 ggcgtggaac gcgtattccc cctgcatact ccccgcattg ccaagattga agtcgtgaac
     5341 cgcggcaaag tccgccgcgc caaacttcac tatctgcgcg gccgcatcgg caaggatgcc
     5401 atgattgtga agtctgccaa ccggtaatcg gaagaattgc tattttgctg gcccggcccg
     5461 ccgtgaggcg gcgccgggtt ttttatttcc ggagtattgt ttttcctgaa tgggaaagag
     5521 gtccacagga acaacagcca cttcaagata tttctccggg agccatatgg ggatgagagc
     5581 gtttttccgg ggtggggaag gtggagaggg gcggctgttt ccggtccgga gcaaatacgt
     5641 ttttcggatt ttcctccgtc cgggcctgga tggcttccgg agaatgga
//
