LOCUS       c00213_gnlPro..         3655 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NCIMCMEO_213.
ACCESSION   c00213_gnlPro..
VERSION     c00213_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 06:58:00
            Original ID  :: gnl|Prokka|NCIMCMEO_213
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3655
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3655
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[306:3655]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      307..3655
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..3655
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3655
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             complement(307..1074)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052:Succinate dehydrogenase-2Fe-2S iron-sulfur
                     cluster binding domain (Score: -1.5; E-value: 8.6)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg213_1"
                     /sec_met_domain="Fer2_3 (E-value: 1.1e-24, bitscore: 74.4,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 3.6e-12, bitscore: 34.5,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 2e-07, bitscore: 19.2,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKTLNLTLKVWRQENREAKGRIETYAAKDIPVDASFLEMLDIVN
                     EGLVKEGKEPIHFDHDCREGICGMCSLTINGIPHGPERQVTTCQLHMRKFKDGDTVWIE
                     PFRAKAFPILRDLMVDRSALDRIIAAGGYIDVRTGSAPNANNIAVEKVKADAAFDAAAC
                     IGCGACVASCKNASAMLFTSAKIAHLNLLPQGQPERSKRVLAMMKQMEAEGFGNCTNQY
                     ECEAACPKGISVDNIARLNRDFMRANAKEGLCF"
     CDS             complement(1115..3073)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 176.1; E-value:
                     4.2e-54)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg213_2"
                     /sec_met_domain="FAD_binding_2 (E-value: 2e-55, bitscore:
                     176.1, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 5.7e-39,
                     bitscore: 120.8, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINFPVSDWMPDAKIPSGPIQDKWSKYKLESKLINPNNKRKYTVL
                     VVGSGLAGASAAATLAELGYNVKCFCYQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRL
                     FYDTVKGGDFRAREANVYRLAEVSSNIIDQCVAQGVPFGREYGGLLDNRSFGGAQLKRT
                     FYARGQTGQQLLLGAYQALEKEVAKGHIEMFSRHEMMDLVLVDGEAKGIVTRNLVTGEI
                     KSYAGDTVLLCTGGYGNVFFLSTNAMGCNVTAAYKAYKRGAFFANPCFTQIHPTCIPVK
                     GDYQSKLTLMSESLRNSGRIWVPKSRETAEKIRKKEIKPSDVPEEERDYYLERKYPSFG
                     NLAPRDISSRAAKEACDDGRGVAVTGKGVFLDFADEIKRMGREWIDGQYGNLFDMYEEI
                     TDDDPHETPMMIYPASHYTMGGLWVDYNLMSNVKGLHVLGEANFSDHGANRLGASALMQ
                     GLSDGYFVIPATLPAYLTTTKPGAVTTAAPEFKAAEDEVKERINKLLNVKGTKTVDSFH
                     RELGLIMWEDCGMARTEESLTHAIERIPQLRKEFWENVRVTGTADGINAELEKACRVAD
                     FLEFAQLLCYDALNRQESCGAHFRVEYQLPDGEAKRNDDEYAYVAAWEYTGVDSLPVLH
                     KEPLTFENVHLAIRSYK"
     CDS             complement(3095..3655)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: SucD_FumR"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg213_3"
                     /sec_met_domain="SucD_FumR (E-value: 5.8e-15, bitscore:
                     43.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="EWINTYAHGLHSMPEAALWGIRAGLAVIFIIHVWLTIQLKLENHA
                     AREPYVFKNTIKATLSSRYMIYTGLTVLVFLIYHLYQYTLRVGYDPAQFTTFISDGTVE
                     TFDVYKMIVTGFSNVWCSAFYILAVLMLFSHLRHGVQSIFQTVGVDSRKIRPFYNFIAI
                     AYGAIICLGFISVPVSVLLGIIK"
ORIGIN
        1 cttgacagga tgccatccct tccgggcatc agtccaattc ctgtaatcaa tgtttctttc
       61 tctcgaagaa agatgggggc cgtcgctgtc ggacgggggc ttgcttaggt ttcgtccggc
      121 atttccaagt acggtctcct ggattactgg aacccttccg gtgtaacaat tttttacgct
      181 gcgcgacgat gaggaagcaa ctgtctggta ttttattcct ggttcaagga aacctggtaa
      241 tgaaaaaact cccgacagcc gatgccgccg ggagttttta cagagcgtct taagaaggaa
      301 tcttctttag aaacagaggc cttccttggc gttggcccgc atgaagtcgc ggttgaggcg
      361 ggcgatgttg tccacgctga tgcctttcgg gcaggcggct tcacattcat actggttcgt
      421 gcagttgcca aagccttccg cttccatctg cttcatcatg gccaggacgc gcttggaacg
      481 ttccggctgg ccctgcggca gcagattgag atgggcgatc ttggcggagg tgaacagcat
      541 ggcggaggcg tttttacagg aggccacgca ggcgccgcag ccgatgcagg cggccgcgtc
      601 aaaggcggcg tccgctttca ctttttccac ggcgatgttg ttggcgttcg gcgcggaacc
      661 ggtacgcacg tcaatatagc ctccagcggc gatgatgcgg tccagggcgg agcggtccac
      721 catcaggtcc cgcaggattg ggaaagcctt cgcgcggaag ggttcaatcc agacggtatc
      781 gccatccttg aacttgcgca tgtgaagctg gcaagtggtc acctggcgtt ccggcccgtg
      841 gggaatgccg ttgatggtca gggagcacat gccgcagatg ccttcccggc agtcgtggtc
      901 aaaatggatg ggttccttgc cttccttgac caggccttca ttgacgatgt ccagcatttc
      961 caggaaggag gcgtccaccg gaatgtcctt ggcggcatag gtctcaatgc ggcccttggc
     1021 ttcccggttt tcctggcgcc agaccttgag agtgagattg agtgttttag ccatgttata
     1081 atctttcttt tgagtgttaa tgtattggct atctttactt gtagctgcgg atggcaaggt
     1141 gcacgttctc gaaggtcagc ggttccttgt ggagaacagg caggctgtcc acgccagtgt
     1201 attcccaggc ggctacatag gcgtattcat cgtcgttgcg cttagcttcg ccgtccggca
     1261 gctggtattc cacgcggaag tgggcgccgc aggattcctg gcggttcagg gcgtcatagc
     1321 agagcagctg ggcgaattcc aggaagtcgg ccacgcggca ggctttttcc agttccgcgt
     1381 tgatgccgtc cgccgtgccg gttacgcgca cgttttccca gaattccttg cggagctggg
     1441 gaatgcgttc aatggcgtgg gtcaggcttt cttccgtgcg ggccatgccg cagtcttccc
     1501 acataatgag gcccagttcc cggtggaagg agtccaccgt cttggtgccc ttcacattca
     1561 ggagcttgtt gatgcgttcc ttcacttcgt cttccgcagc cttgaattcc ggggcggcgg
     1621 tggtgacggc gccgggtttg gtggtcgtca ggtaagcggg cagcgtggcg ggaatgacga
     1681 agtagccgtc agacaggccc tgcatcaggg cggaggctcc caggcggttg gcgccgtggt
     1741 cggagaagtt ggcttcaccg aggacgtgca gccccttcac attggacatg aggttgtagt
     1801 ccacccacag gccacccatg gtgtagtggg aggcggggta gatcatcatg ggcgtttcat
     1861 gggggtcgtc gtccgtgatt tcttcgtaca tgtcaaacag attgccgtat tggccgtcaa
     1921 tccattcgcg gcccatgcgc ttgatttcat ccgcaaaatc taggaagacg cccttgccgg
     1981 tgacggccac gccgcggccg tcgtcgcagg cttccttggc ggcgcgggag gaaatgtcgc
     2041 gcggagccag gttgccgaag gagggatact tgcgttccag gtagtagtcc cgttcttctt
     2101 cgggaacatc ggaaggcttg atttcctttt tacggatttt ttctgcggtt tcacgggact
     2161 tgggcaccca gatgcggcca ctgttgcgga gggattccga catcagcgtc agtttggact
     2221 gatagtcgcc cttgaccgga atgcaggtgg ggtggatttg cgtgaagcag gggttggcga
     2281 agaaggcacc gcgcttgtag gccttgtagg cggcagtgac gttgcagccc atcgcgttgg
     2341 tggacaggaa gaacacgttg ccgtagccgc ccgtgcagag caggacggta tcccctgcgt
     2401 aggatttgat ttcgccggta accagattgc gggtgacgat gcccttggct tcgccgtcca
     2461 ccagcaccag atccatcatt tcatggcggg agaacatttc aatgtgtccc ttggccactt
     2521 ccttttccag ggcctggtag gcacccagca gtagctgctg gcccgtctgt ccgcgtgcgt
     2581 agaaggttcg cttgagctgg gcgccgccga aggagcggtt gtccagcagg ccgccgtatt
     2641 cacggccgaa gggaacgccc tgggccacac actggtcaat gatgttggag gagacttccg
     2701 ccagacggta gacattggct tcacgggcgc ggaagtcgcc gcctttaacg gtgtcgtaaa
     2761 acaggcggta aacggagtcg ccgtcgttct ggtagttctt ggctgcgtta atgccgccct
     2821 gggccgcaat ggagtgggcg cggcgggggc tgtcctggta gcagaagcat ttgacgttgt
     2881 agccaagttc ggcaagcgtg gcggcagcgg aggcgccagc caggccggaa cctaccacaa
     2941 gcaccgtgta cttgcgcttg ttgttcggat tgatcagttt ggactcaagt ttgtacttgg
     3001 accatttgtc ctgaataggg ccggagggaa ttttggcatc cggcatccag tcgcttacgg
     3061 gaaaattaat catgactttg agaggagttg atggttattt gatgataccc agaagaaccg
     3121 agaccggcac ggagatgaag cccaggcaga taatcgcgcc gtaggcgatg gcgataaagt
     3181 tgtagaaggg ccggattttg cgggaatcga cgcccacagt ctggaagatg gactgtaccc
     3241 cgtggcgcag atggctgaac agcatcagaa cggccaggat gtagaaggcg gagcaccata
     3301 cgttggagaa tccggtcacg atcatcttgt acacgtcaaa ggtttccacc gtaccgtcgg
     3361 aaatgaaggt ggtgaactgg gcggggtcgt atcccacgcg cagggtgtac tggtacaggt
     3421 ggtagatcag gaacaccagg acggtcaggc cggtatagat catgtagcgg gaggagagcg
     3481 tcgccttgat ggtgttcttg aacacgtagg gttcgcgggc cgcatggttt tccagcttca
     3541 gctggatggt cagccagacg tggatgatga agatcaccgc aagccccgcg cggattcccc
     3601 agagggctgc ttccggcatg gagtgcaggc cgtgggcgta ggtgttgatc cattc
//
