LOCUS       c00131_gnlPro..         6956 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|MLBNNDOO_131.
ACCESSION   c00131_gnlPro..
VERSION     c00131_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 07:09:05
            Original ID  :: gnl|Prokka|MLBNNDOO_131
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6956
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6956
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:2045]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2..2045
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..6956
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6956
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..1318
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg131_1"
                     /sec_met_domain="Gly_radical (E-value: 9.4e-48, bitscore:
                     149.4, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 6e-185, bitscore:
                     604.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="HLRMQSYNDIFAGDPTWVTEAIGGRFNDGRVKVTKTSFRFLQTLY
                     NLGPSPEPNLTVLWSPELPEGFKDFCAKVSVDTSSIQYENDNLMREVRNCDDYGIACCV
                     SYQAIGKQIQFFGARANLAKALLLAINGGRCENTGTVMVKGIPVLTGETLKFEEVMANY
                     KKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMAFIDTNPRINLAYGVAGLSIAIDSLSA
                     IKYAKVTARRNEIGLTEGFDTEGSFPCFGNNDDRVDHLGVDLVYYFSEELKKLPVYKNA
                     RPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIASLSSVAKLRY
                     RDSQDGISNTFSIVPKSLGPTPEERVENLVTMMDGYFTKGAHHLNVNVLNREMLEDAME
                     HPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             1320..2045
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 75.5;
                     E-value: 1.7e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg131_2"
                     /sec_met_domain="Radical_SAM (E-value: 7.3e-32, bitscore:
                     99.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCPFRCLYCANPDTIDVKGG
                     TPTTPEEILQMAISQKAFFGKKGGITFSGGEPTLQAEALIPLFKELKANGIHICLDSNG
                     GIWNEKVEELFSLTDLVLLDIKQFNPERHRTLTGRSNEQTLRTAAWLEEHEHPFWLRYV
                     LVPGYSDSEEDIRLLGESLGKYRQIQRVEILPYHRLGVHKYEVMGWDYKLKEVKENTPE
                     QLERAEKLFKEYFTNVITN"
     CDS             complement(2114..4318)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fn3-like"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3_C"
                     /gene_functions="other (smcogs) SMCOG1041:Fibronectin type
                     III-like domain (Score: 87; E-value: 2.2e-27)"
                     /gene_kind="other"
                     /locus_tag="ctg131_3"
                     /sec_met_domain="Fn3-like (E-value: 1.4e-28, bitscore:
                     87.0, seeds: 729, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3 (E-value: 1.5e-75, bitscore:
                     242.6, seeds: 11, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3_C (E-value: 2.7e-53,
                     bitscore: 169.1, seeds: 116, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKIIILMVCLWGLGNTFTSAQTTEKEKFISSLMKQMTLDEKIGQ
                     LAQCTYRGNFTGPDGGNIPKEEYVRQGRIGSMLNVIGVKDIRRYQELALQSRLRIPLIF
                     GLDVIHGYRTGFPLPLAEAASFDLAMIEEAARYNALESAADGLNWVFAPMVDISWDARW
                     GRVMEGAGEDPYYGSRVAEARVRGLQGDDLADTTTVMACMKHFAGYGAPIAGKEYNSVD
                     MSMGHFANFYMPPYKAAIKVGAATVMSAFNDFNNIPCTANDFLLNRLLREQWGFKGFVV
                     SDYNSVEELVNHRYAVDKKDAAAKALNAGLDMEMVSTCYLTYLKELVQEGKVKESVLDD
                     AVRRILEKKYELGLFEDPFRYCNPERAARILNAPEVRNASLRMAERSVVLLENRESVLP
                     LTAQTRKIALIGGLSKSQYDMAGAWAATTDRSKVVTLYDALVRKGLEVNYAEGYDMKSN
                     QLTGLQEALQAAEASDVIVVAVGERFGQSGEKASKVNIAIPEGQQKLVSELAKTGKPVI
                     ALVMCGRPVIFNEIREHADAVLCTWWLGSEAGNAICNVLWGEYNPSAKLPMTFPAHVGQ
                     IPIYYQNKSTGRPTALQQTYKASYIDASTEPAYPFGYGLSYTDFGYSDLKLLPGKDAGV
                     HTEVSVKVTNTGKCAGEEVVQLYVQDKVASVTRPIKELKGFRKIHLNAGESKTVAFQIT
                     DDALGFYDNEMNYMVEPGDFVFMVGGSSDDVQQITYNLKE"
     CDS             complement(4349..6811)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fn3-like"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3_C"
                     /gene_functions="other (smcogs) SMCOG1041:Fibronectin type
                     III-like domain (Score: 80.1; E-value: 3.4e-25)"
                     /gene_kind="other"
                     /locus_tag="ctg131_4"
                     /sec_met_domain="Fn3-like (E-value: 2.2e-26, bitscore:
                     80.1, seeds: 729, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3 (E-value: 2.3e-57, bitscore:
                     182.8, seeds: 11, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3_C (E-value: 1.5e-51,
                     bitscore: 163.4, seeds: 116, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIKKKVALSGLLLCCAITAKAQVCITPQAEQRARELVSQMTLEE
                     KIDYISGPKSFYIRAVPRLGIPEIRMADGPQGIRNNTQSTLYPCGILSASTWNRKLARE
                     LGHGLARDAKARGVSILLGPGVNIYRSPLCGRNYEYFGEDPYLTGETAKEYILGVQEEG
                     VMATVKHFAANNQEWSRHHASSDVDERTLQEIYFPAFRKAVQEAGVGAVMDSYNPLNGV
                     HATENSWLNIDVLRKQWGFKGILMSDWTSVYSGVGAANGGLDLEMPVGKFMTREILIPA
                     IENGIVKEETIDAKVRHILQTLIAFGALDTPREDKSIDKDNAQSKEIALDLAREGVVLL
                     KNDNGTLPYRNGRTLVIGPNADIIPTGGGSGFVTPYSVVSLYDGMVQLKGGRQIELLSD
                     SILYKDMSQQIYTDGSFTQNGFKGEYYNTRNLTGELFQAAIEPAIDHAWKYGAPFDGMP
                     VDQFSARWTGVYKADKDGTVKFQLAGDDGYRLFVNDKLITGDWGNHSFSTRSAFMQVSA
                     GEIYRLRIEYFDNVGEATVSFQAGMMDEERLASSLKHARNVIVCAGFNSSTEGEGFDRP
                     FALSYGQEYLINKVASLHDNVAVVVNAGGGIDFRNWGQSVQAILMAWYPGQEGGKALAE
                     IITGKLSPSGKLPVSIEEKWEDNPVYGNYYDNRNVPHKRVQYAEGVFVGYRGYDRNGKQ
                     PLYPFGYGLSYSSFEYSNLSVEKTGGSRVTVSFDIKNTGKMDAAEAAQVYVRDVECSVP
                     RPLKELKGYDKVYLKKGETKRVRILLDEEAFAYYDVESHRFVVEKGTFEILAGPSSADL
                     PLKATVVL"
ORIGIN
        1 ccatctgcgt atgcagtcgt acaatgatat ctttgccggt gacccgactt gggtgaccga
       61 agccatcgga ggacgcttca atgacggacg cgtgaaagtc accaagactt ctttccgctt
      121 cttgcagacg ctatacaacc tcggtccttc tccggaacca aacttgaccg tactgtggag
      181 tcccgaactg ccggaaggct tcaaggattt ctgcgccaaa gtttccgtag ataccagctc
      241 catccagtac gaaaacgaca acctgatgcg cgaagtgcgc aactgcgacg attacggcat
      301 tgcctgctgt gtatcctacc aagccatcgg caagcaaatc cagttcttcg gcgcacgtgc
      361 caaccttgcc aaggccctgc tgcttgccat caacggtggc cgttgcgaaa acaccggaac
      421 tgtcatggtg aaaggtattc ccgtattgac cggcgagaca ctgaagtttg aggaagttat
      481 ggccaactac aagaaggtac tgactgaaat agcccgtgtc tacaacgagg ccatgaatat
      541 catccactac atgcatgata agtattatta tgagaaggcc cagatggcat tcatcgatac
      601 caacccacgc atcaatctgg catacggtgt tgccggcctt tccatcgcca tagactcact
      661 gtccgccatc aaatatgcca aagtcactgc acgccgcaac gaaatcggcc tgaccgaagg
      721 tttcgacact gaaggctcat tcccctgctt cggcaataat gacgaccgtg tggaccacct
      781 gggagtagac ctcgtttact atttcagcga agaactgaaa aagctgcctg tctacaagaa
      841 tgcccgtccc accctttcct tgctgactat cacctccaac gtgatgtacg gcaagaagac
      901 cggtgccact ccggacggac gcgccaaagg tgttgccttt gctccgggtg ccaacccaat
      961 gcacggacgt gacaagaacg gtgccatcgc ttccttgagc tccgtagcca aattgcgcta
     1021 ccgcgactcg caggacggca tcagcaatac gttctccatt gttcctaaat cactgggccc
     1081 gacaccggaa gaacgtgtag agaacctggt aaccatgatg gacggctact tcaccaaagg
     1141 tgcccaccat ttgaacgtaa acgtactgaa ccgcgaaatg ctggaagatg ccatggaaca
     1201 tcctgaaaag tatccgcaac tcaccatccg cgtttccggc tatgccgtga acttcgtgaa
     1261 gttgagccgc gagcatcagt tggaagtcat cagccgttca ttccacgaaa gaatgtaata
     1321 tgataaatgt acactcctac gaaagtatgg gaacattcga cgggccgggt ctccggctcg
     1381 tcgttttcct gcaaggatgt ccgttccgtt gtctgtattg tgccaatccg gatacgattg
     1441 atgtaaaagg aggcactccc accactccgg aagagattct gcaaatggct atcagccaaa
     1501 aggcattctt cgggaagaaa ggaggtatta ctttctccgg cggtgagcct accctgcaag
     1561 cagaagcgct gattcctctt ttcaaggagc tgaaggcgaa cggcatccat atctgtctgg
     1621 acagtaacgg aggcatctgg aatgaaaagg tggaagagct ctttagcctg actgacctcg
     1681 tgctgctgga catcaagcaa ttcaatcccg aacgccaccg tacgctgacg ggacgaagca
     1741 acgaacagac actccgcacc gctgcctggc tcgaagaaca tgaacacccc ttctggctgc
     1801 gttatgtact ggttcccggt tacagcgatt cggaagaaga catccgcctg ctgggtgaaa
     1861 gtctcggcaa ataccggcag atacaacggg tggaaatact tccttaccac cgtctgggcg
     1921 ttcacaaata cgaagtcatg ggatgggatt acaaattgaa agaagtgaag gaaaatactc
     1981 cggaacaact ggaacgggca gaaaaactgt tcaaagagta tttcacgaat gtgataacca
     2041 actaaaacag aaaagcaagg ctgtccggat atgattccag acagccttgc ttttcattta
     2101 taggacaaga atcctattct ttcaagttat aggtaatctg ctgtacatca tcactactgc
     2161 cacccaccat aaagacgaaa tcaccaggtt ctaccatata attcatctca ttgtcataga
     2221 atcccagtgc atcgtcagtt atctgaaaag ctacagtttt gctttcaccc gcattcagat
     2281 gaattttcct gaagcccttc aactccttta tcgggcgcgt aacagacgct accttatcct
     2341 gcacatacaa ctggactact tcctctcctg cacacttacc ggtattagtc acttttaccg
     2401 acacctccgt atggacgcct gcatccttgc ccggcagcag tttcaaatca gaatatccaa
     2461 aatccgtata cgaaagccca tatccgaaag gataggcagg ctcagtggaa gcatcgatat
     2521 aggatgcctt ataagtctgt tgcaatgcgg taggacgacc ggtactctta ttctggtaat
     2581 aaatgggaat ctgccctacg tgtgcaggaa atgtcatggg caatttagcc gaaggattat
     2641 actctcccca caacacattg catatggcat tgccggcctc actccccagc caccaagtac
     2701 acagtaccgc atcagcatgt tcccttatct cattgaagat taccggacgc ccacacatga
     2761 caagagcaat gacaggtttt ccagtctttg ccagttccga caccagcttc tgctgcccct
     2821 cgggtatggc tatattcact ttggaagcct tctcgccact ctgaccgaat ctctcgccta
     2881 ccgccacaac aatgacatcc gaagcctcag cagcctgcaa tgcttcctgc agtccggtca
     2941 attgattgct cttcatatca tagccttcag catagttcac ctccagtcct ttccggacca
     3001 gtgcatcata caaggtcacc actttgctcc ggtccgtagt ggcggcccaa gcgcctgcca
     3061 tatcgtattg ggattttgag agtcctccta tcaaggctat cttcctcgtc tgtgccgtca
     3121 acggaagcac agattcccta ttctccagca atacaacgga acgctcggcc atacggagcg
     3181 aagcattacg gacttcgggc gcattgagta ttctggccgc tctttccgga ttacagtagc
     3241 gaaaagggtc ctcaaacaat cccaactcat atttcttctc caatatacgc cgtactgcat
     3301 catcaagcac actctccttt actttccctt cttgaacaag ctccttcaga taagtcaagt
     3361 agcaggtact taccatctcc atgtccagac cagcattcag cgctttggct gcggcatctt
     3421 tcttgtcaac agcataacgg tggttcacca attcctctac cgaattataa tcggacacaa
     3481 caaatccctt aaatccccac tgctctctca atagccggtt gagcaagaaa tcattggcag
     3541 tacaaggaat attattaaaa tcattgaaag cactcatcac ggtagctgct cctaccttta
     3601 tggccgcctt atagggaggc atataaaaat tggcaaagtg gcccattgac atatccacac
     3661 tattatactc ctttcctgct atcggtgcac catatccggc aaaatgtttc atacatgcca
     3721 tgacagtcgt cgtgtctgcc aaatcgtctc cttgcaagcc tctgacacgc gcctccgcta
     3781 cccgagaacc ataataaggg tcctcaccgg ccccttccat gacgcgtccc caacgtgcgt
     3841 cccacgaaat atccaccatc ggggcaaaaa cccagttcaa accatcggcg gcactttcca
     3901 aagcattgta gcgtgcagct tcctcaatca tggccaaatc gaaacttgca gcctcggcaa
     3961 gcggaagcgg aaatccggta cgatagccgt ggataacatc cagtccgaaa attaaaggaa
     4021 tgcgcaaacg cgattgcaaa gcaagctctt gataacggcg tatatccttt actcctatca
     4081 cattcagcat agagcctatt ctgccttgcc ggacatactc ttctttagga atatttccac
     4141 cgtcgggtcc ggtaaaattc cctcggtagg tgcattgtgc aagctggcct atcttttcat
     4201 ccagcgtcat ctgcttcatc aagcttgaga tgaatttctc cttctccgta gtttgtgccg
     4261 aggtaaaggt gttaccaagt ccccacagac aaaccatcag tattattatc tttttcatac
     4321 tgccattctt gttttgatat ttattttatc ataaaacaac agttgccttc aatggtaaat
     4381 cagctgaaga aggacctgcc agaatctcaa aagtcccctt ttccactaca aaccgatgtg
     4441 attccacatc atagtaggca aaggcttcct catccagcaa tatccgaacg cgcttggttt
     4501 cacccttctt caagtaaacc ttatcgtagc ctttcaactc cttcaatgga cgcggaacag
     4561 aacattccac atcccgcaca tatacctgag ccgcttccgc agcatccatc tttccggtat
     4621 tcttgatgtc aaagcttacc gtaacccggc ttccccccgt cttctcaaca ctcagattac
     4681 tatattcaaa actactgtat gacagtccat acccgaaagg ataaagcggc tgctttccgt
     4741 tccggtcata tcccctataa cctacgaaaa caccttcggc atactgcacc cgtttatgag
     4801 gtacattgcg attgtcataa taattaccat ataccggatt atcttcccat ttttcctcga
     4861 tgctgactgg tagtttgcca cttggcgaaa gcttgcccgt aatgatttcg gcaagggcct
     4921 ttccaccctc ctgccccgga taccatgcca tcaaaatagc ttgtacactc tgcccccaat
     4981 tgcggaaatc tattccacct ccggcattca ccaccacagc cacattatca tgaagtgaag
     5041 ccactttatt aatgagatat tcctgcccgt atgacaatgc aaacggacgg tcgaaccctt
     5101 caccttctgt actgctgttg aacccggcac atacaataac attcctggca tgcttcagtg
     5161 aagaggccaa acgctcctca tccatcattc ctgcctggaa actgacagtc gcctcaccca
     5221 cattatcaaa atattcaata cgcaaccggt atatctcccc tgcactgacc tgcataaatg
     5281 cactgcgggt actgaatgaa tggttccccc aatctccggt aatcagctta tcgtttacaa
     5341 acagacggta tccgtcatcg ccagccagct ggaacttgac agttccatcc ttgtcagcct
     5401 tgtaaactcc cgtccaacgg gcagaaaact gatctaccgg catgccgtcg aacggtgcac
     5461 cgtatttcca ggcatggtct atggcaggct ctatggcagc ttgaaagagt tctccggtaa
     5521 gattacgagt attataatat tcgcccttaa aaccgttttg cgtaaaactg ccatccgtat
     5581 aaatctgttg ggacatatcc ttataaagaa tgctgtcaga cagcagttct atctgtctgc
     5641 cgcccttcaa ctgaaccata ccgtcataga gcgacactac cgaataagga gtgacaaatc
     5701 cgcttcctcc tcctgtggga ataatatcgg cattaggccc gattaccaat gttctaccgt
     5761 tccggtaagg caacgtaccg ttgtcatttt tcaataacac tactccttcc ctcgccaagt
     5821 caagtgcaat ttccttagac tgtgcattgt ctttatcgat agatttatct tctctcggag
     5881 tatcaagtgc tccaaaagca atcaatgtct ggagtatatg ccggactttg gcgtcaatgg
     5941 tctcttcctt cacgatacca ttctctattg ccggaataag aatctccctg gtcatgaatt
     6001 taccgacggg catttccaaa tccaaccctc cattagcggc tcccacaccg gaatatacag
     6061 atgtccagtc agacatcagg atacctttaa atccccactg cttgcgtaat acatcaatgt
     6121 tcagccagct gttttcggtg gcatgcaccc cgttcagcgg attatagcta tccatgaccg
     6181 ctcctactcc tgcttcctgc acagcttttc ggaacgcggg aaaataaatt tcctgcaacg
     6241 tgcgttcatc cacatccgag ctggcatgat gcctgctcca ctcctggtta ttggctgcaa
     6301 aatgcttcac cgtagccatg actccctctt cctgcactcc caagatatat tctttggctg
     6361 tttcgcccgt cagataaggg tcttcaccaa aatactcata gtttcgcccg catagaggag
     6421 aacggtagat attgactccg ggccccaaca gaatacttac cccacgggcc tttgcatccc
     6481 gggccaaacc atgtcccagt tctctagcca acttacggtt ccatgtcgaa gccgacaaga
     6541 taccacaagg atacagggta ctttgagtat tgttacgtat gccttgcgga ccatcggcca
     6601 tcctgatttc gggtatgcca agccggggca cagcccggat ataaaaagat ttgggaccgg
     6661 aaatgtaatc gattttttct tccagagtca tttgagaaac caactccctg gcacgctgct
     6721 ccgcctgggg agtaatacac acttgtgctt ttgccgtgat tgcacaacaa agcaatagcc
     6781 cgcttaaggc tactttcttt ttaatcttca ttttgtctat tttactacgt gtttgttata
     6841 ttgccgggca tgtcccgacc tactttcttt cttctcaatc aataaaaaac cgtgtccctt
     6901 catccaccaa cgaagaaaca cggccgaaag ttattaagaa aattctgaat ctacaa
//
