LOCUS       c00383_gnlPro..         3040 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|IHCBOJKA_383.
ACCESSION   c00383_gnlPro..
VERSION     c00383_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 12:40:16
            Original ID  :: gnl|Prokka|IHCBOJKA_383
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3040
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3040
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[25:3001]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      26..3001
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3040
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3040
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(26..760)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 67.6;
                     E-value: 4.7e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg383_1"
                     /sec_met_domain="Radical_SAM (E-value: 2.6e-34, bitscore:
                     106.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWI
                     RHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPL
                     SKERLDNAKAIFSRYKPENNKA"
     CDS             complement(761..3001)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg383_2"
                     /sec_met_domain="Gly_radical (E-value: 7.8e-47, bitscore:
                     145.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.2e-282, bitscore:
                     925.8, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENECWDHFKGETWKREINVRDFIQSNYTPYEGDDSFLVASSE
                     KTRRVWNKLTEMFKVEREKGVYDAETKLPQGIDVYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTEEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTAILIEEKKRFLNRLDIQEITEEIIQSREEISEQIKAL
                     KAFEKMCASYGFDVTRPAQNAREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEK
                     DIREGKLTEEEAQELVDQLIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTRTSYRYLHTLYNLGPAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRG
                     EYLEYDEVMAKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFM
                     EYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGA
                     IAALASVAKLPFQHAHDGISYTFAVSPATLGKEREVQINNLVSLLDGYFTPDGGQHLNV
                     NVFDKDLLIDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
ORIGIN
        1 ataatcgcaa ggcttgatcg agagatcagg ccttgttatt ttctggtttg tagcgagaga
       61 atatcgcttt cgcattatct aaacgttctt ttgataaggg ttccaccccc tttaacggat
      121 aatcgagacc taattgctca tatttatagg ttcccatcgt atgataaggc aataattcca
      181 ctttgcgaac cactttatat gaactgacat accgagctaa cgcctccagc cactcgtcat
      241 tatccgtata tccggggaca ataacatggc gaatccaagt atctatgcca cgtctctcca
      301 attcatccaa gaacagaagc gtattatcct gcttgacacc cgccagtaac ggatgtaaat
      361 ccgggtttaa ggttttgatg tccaataaca ctaagtccgc ataatccaag acttcccaag
      421 cttttgaggt acatacgaaa ccagatgtgt ccaaagctgt gtgaagacct tgctcttggc
      481 acaaacggaa aaactctttc agaaactcag gttgcaataa aggttcccca ccggtaacag
      541 tcaccccccc acgggcgata aaactcttat accgtaagac ttccgtcaat agctcgccgg
      601 gagtcatctg atatttcacc ttccctttcg ggttccaagt atccgggtta tggcaataca
      661 ggcaacgcaa cgggcagccc tgcataaaaa cgacaaagcg aattccggga ccatctacgg
      721 tcccgaaact ctctaatgaa tgtatcttac cttttatcac ttacagtgaa tggttaatgg
      781 ttcttgaaat aacatccaac tgttgttcac gagtcagttt cacgaagttt accgcatatc
      841 cggatacacg gatcgtaagt tgcggatatt tctcaggatg ttccatagcg tcgatcaaca
      901 aatccttatc aaatacgtta acattcagat gctgacctcc atccggagta aaatagccat
      961 ccaataagct aaccagatta ttgatctgca cttcacgctc ctttcccaac gtagccggag
     1021 aaacagcgaa agtataagag ataccgtcgt gcgcatgctg gaatggaagc ttcgctaccg
     1081 aagctagcgc agcgatagca cctttcgtat cacgaccgtt catagggttc gcgcccggag
     1141 cgaaaggagt tccaccgata cggccatccg gtgtcgtacc agtcttctta ccatatacca
     1201 cattagaggt gatcgtaagg atggactgcg taggtttcgc atggcgatag gtctcgtgct
     1261 gacgcaaata ttccatgaac ttctcggtga tctctacagc gatactatcc gtacgatcgt
     1321 cgttattacc gaaaggaaca tattcgccct cacgttcgaa atcaaccgcc aagccacgct
     1381 cgtcacggat cacacgtact ttcgtatcac ggatagcagc caacgaatcg gcaacgatag
     1441 aaagaccggc gatacctgtc gcacggatac gctctacatc tccatcgtga agcgacatct
     1501 cgaatgcctc atatgcgtat ttatcatgca tataatggat tatcttcaag gcgttcacat
     1561 acactttcgc caaccagcgc atcatttgct catacttagc catcacctca tcgtattcca
     1621 gatactcgcc gcgaaccggc tcgaacatcg gggctacctg tacgccgctg cgctcatccc
     1681 gtcctccgtt gatcgcatat aataaacatt tcgccaaatt cgcacgggct ccgaagaact
     1741 gcatctgctt accgatcttc ataggagaaa cgcaacaagc gataccataa tcatctccat
     1801 aatccggacg catcaaatcg tcgttctcat attggatagc cgatgtatcg atagagacct
     1861 tggcacagaa acgtttccag ttctcgggag cgttcttaaa ccacagaacc gtcaaattgg
     1921 gttctggtgc cggacctaga ttatataatg tatggagata acgataggaa gttcttgtta
     1981 ccaaagaacg gccatccact ccttggccgc ccaaggactc tgttacccaa accggatcac
     2041 cggagaacaa atcattgtat tccggcgtac gtaagaaacg aacgatacgc aacttgatga
     2101 tcagctggtc taccaattct tgcgcctcct cttcggtcaa cttaccctca cggatgtctt
     2161 tctcgatata aatatccaaa aacgtagaag tacgtccgat agacatcgcc gcgccatctt
     2221 ggtcttttac cgccgccaga taagctaagt acacaaactg aactgcctca cgagcgtttt
     2281 gtgccggacg agtcacatca aagccatagc tagcgcacat tttctcgaac gctttcaaag
     2341 ccttgatctg ttcggaaatc tcctcacggc tctggatgat ctcttccgtg atctcttgga
     2401 tatccaaacg attcaagaaa cgctttttct cctcgatcaa gattgccgta ccatataaag
     2461 cgacacgacg atagtcacca ataatacgtc ctctaccata cgcgtcgggc agaccggtga
     2521 taatagccga acgacgtgcg gcaatcatct cttccgtata tgcggagaaa acgccttcgt
     2581 tatgagtttt acggtacttc gtgaagatct ctttcgtcat cgggtccaaa tgataaccat
     2641 aagcttccaa gctattctca accatacgga taccgccctt cgggaagata ccacgtttca
     2701 gcggagcatc ggtttgtaac ccaacgatca cctcattctc cttatcaata tagcccgggc
     2761 catatacatc gatcccttga ggtaattttg tctcggcgtc ataaactccc ttttccctct
     2821 cgaccttaaa catctcggtc agcttgttcc agaccctacg ggtcttctcg gaagaagcga
     2881 ctaaaaagga atcatcccct tcataaggcg tatagttact ctggataaaa tctcttacat
     2941 tgatctctct tttccaggtc tcacctttaa agtgatccca gcactcattc tcaaatttca
     3001 taaacaatct tatctaatac cacattttta ccgccgcaaa
//
