LOCUS       gnlProkkaLOFFKKHD_15   13057 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|LOFFKKHD_15.
ACCESSION   gnlProkkaLOFFKKHD_15
VERSION     gnlProkkaLOFFKKHD_15
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-12-01 23:57:25
            Original ID  :: gnl|Prokka|LOFFKKHD_15
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 13057
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..13057
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[4488:8057]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      4489..8057
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     cand_cluster    1..13057
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..13057
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="phosphonate"
                     /region_number="1"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     CDS             3..263
                     /locus_tag="ctg15_1"
                     /transl_table=11
                     /translation="MKGKDKVLHICKLLSADTYYNAIGGKQIYNKEDFMQNGINLKFVD
                     TIIKRYPQQSKEFVPYLSIIDVLMNNSKEETCMLLDSFSLQ"
     CDS             263..1225
                     /locus_tag="ctg15_2"
                     /transl_table=11
                     /translation="MSIGGYFELEIREGEHFHQGAIKLSTARQCFEYILKARRYTKVYI
                     PYYTCDVIIEPLMKLNVKYEYYHINSLLDPVFDKKIESSEAFLYTNYFGLKQDTVLYLS
                     RFYPNLIVDNAQAFFAKPIEGVDTFYSARKFFGVPDGAYLYTTALLDIDFPIYNPIDNI
                     NYLIGRITDSAEGYYKDFQESEERMSNQEIHRMSVLSDKILSSLDYVSIIERRRFNYHL
                     LYSALTAFNKFDFSISENQNVPLIYPFYTSKAGLRQQLILEHVYVPQYWQNVVNKIDAK
                     CLEYSIANNMIALPIDQRYDEKTMNILIEKISNLNNHLS"
     CDS             1222..2217
                     /NRPS_PKS="Domain: NAD_binding_4 (91-233). E-value: 3e-07.
                     Score: 21.7. Matches aSDomain:
                     nrpspksdomains_ctg15_3_NAD_binding_4.1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score:
                     114.5; E-value: 1.1e-34)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg15_3"
                     /transl_table=11
                     /translation="MKKKIIIFGATGNTGSYLLEYALNFFNKEEYEIIASGRRETTFFS
                     KRGIPYYSVDITKEEDFDKLPMENIHAVMLLSAQIPSYMDTYCPIKYLDSIVIGGFNVL
                     EYCRKCHVDRIIYTQTVFDISLYSHDTILKPDMKPNFSYTGDHAIYVISKNTALDLIEH
                     YYQEYGLKKFIFRLPTIYSYSPYPYYFPNGVKTKRPIYQMIENAQKGLPIEIWGDPHYA
                     KDMVHVNDFSQMLCKAVEVEQESGFYNVGTGMPITLEEQIRTIISVFSPKESPSEVIYR
                     PDKVCGGGYIMNIENAKKELGYVPQYDCRKLFEDYKSEMEIKRFAELRLK"
     aSDomain        1495..1920
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg15_3_NAD_binding_4.1"
                     /evalue="3.00E-07"
                     /label="ctg15_3_NAD_binding_4.1"
                     /locus_tag="ctg15_3"
                     /protein_end="233"
                     /protein_start="91"
                     /score="21.7"
                     /tool="antismash"
                     /translation="YLDSIVIGGFNVLEYCRKCHVDRIIYTQTVFDISLYSHDTILKPD
                     MKPNFSYTGDHAIYVISKNTALDLIEHYYQEYGLKKFIFRLPTIYSYSPYPYYFPNGVK
                     TKRPIYQMIENAQKGLPIEIWGDPHYAKDMVHVNDFSQ"
     CDS             2218..3150
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg15_4"
                     /sec_met_domain="Glycos_transf_2 (E-value: 3.6e-25,
                     bitscore: 80.8, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNGSITVSICCLTYNHEKYIRQCLDGLIMQKTEFDFEILVHDDAS
                     SDATQSIINEYVVAYPNLIKPILQIRNQYSQGISPLRQILFPLVKGKYVALCEGDDYWT
                     DPLKLQKQVNAMESDHSLAGCAHQSLVFYENGTPSHMFHHVKKQILKTKDLLDVRLFHT
                     ASFLFKKEVLDYIKDFNSSLYAGDRFLFLACSLKGNILYMPDVMCCYRKNAMGVTSRVS
                     YSLHVLDLKVPEYFHNKDLKFPYYRYLAFVHRAIFSESLDISLKKYIYHFTLFVKYSFS
                     YFPKNLKDIYKALYVSFWWLTKGQKYRIK"
     CDS             3324..4526
                     /locus_tag="ctg15_5"
                     /transl_table=11
                     /translation="MLLLINVWAFNWGFLCFGKIKKIRLKVTTDNSFYNLVIGNNKMIL
                     LLMLACIVTYYYAMKFALVIELYGAGEGRMMRFEAGELFTSNLEVYLYDFLIKPFTQFV
                     MLIVACMLLSKIKRRILLLSVLFLFLYASVGNGRGGLIVFLFYCFIIYYLRRELYYINL
                     LASKRKKWNFGVVSFVCLFVGVGIIVVVISYMTAQRMGFDTFSAEVVKIGFEELKSQAV
                     WYFLGPFRALDYSFRANYESITGFWYGRATMGGIDNLLKLVIHYLGGSYTIAHDEIGHL
                     LQNSKISVGNGIIFNYAYTNIIFHYLDFGYVGVLLFPFLYGFIVRYSIYKFTQKPTLLT
                     LGIMIYLVYISMFNIFSWEFQKPSSIIYVLLLLFFQYVYSHKFVILKLNGKKKGLCRHE
                     C"
     CDS             4489..5793
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: CTP_transf_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231:carboxyvinyl-carboxyphosphonate (Score: 202.6;
                     E-value: 1.3e-61)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg15_6"
                     /sec_met_domain="CTP_transf_like (E-value: 1.6e-12,
                     bitscore: 39.8, seeds: 65, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 4.2e-119, bitscore:
                     388.9, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 1.4e-63, bitscore:
                     206.6, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKKKVYVGMSADIIHPGHLNIIHEASKLGHVTVGVLTDSAIASY
                     KRLPYLNFDQRSLIVLNLKGVDEVIPQTTLDYVPNLELIRPDFVVHGDDWKEGIQQETR
                     QRVIDTISKWGGKVIDVPYTKGISSTLLNTRLKEIGTTPEIRLKRLKRLIAAKSIVRIC
                     ESHSGLTGLIIENTSVEVNGVKREFDGMWSSSLTDSTSKGKPDIEAVDLTTRLHDLNDA
                     LECTTKPVIFDGDTGGKIEHFVFTVRTLERLGISAVIIEDKIGLKKNSLFGTDAVQTQD
                     TIEGFCDKIQAGKRAQITDDFMIIARIESFIAGKGLDDALGRALAYIKAGADGIMIHSK
                     DKSGEDIKNFCLALRKIHKSIPIVVVPTTYNHISEDELASWGVNVVIYANHMLRSAYPA
                     MLNTAKSILANARSYEANDLCMPIKEILELIPGTK"
     CDS             5825..6955
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055:pyruvate oxidase/decarboxylase (Score: 69;
                     E-value: 6e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg15_7"
                     /sec_met_domain="TPP_enzyme_N (E-value: 9.3e-17, bitscore:
                     53.2, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 1.5e-20, bitscore:
                     65.5, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MISPNIFVDSLGAMQMDFFTGVPDSLLKNICAYLTDHLASEHNII
                     AANEGAAIGLATGYYLATRKIPVVYMQNSGLGNAVNPLMSLTDKEVYNIPLLLLIGWRG
                     EPGVKDEPQHIKQGKVTLPLLEAMGIRYAVLSQDEKKFISQLNVAKSYMDETREPFAFV
                     IQKDTFLEYSSENCEDERLSLKREAAIHFIAASLTEKDIIISTTGMISRELFEYRESTS
                     LEHERDFLTVGSMGHASQIALGIALKKRDCKVYCFDGDGSTLMHMGSLAIVGSLHPENY
                     VHVVFNNGAHDSVGGQPTVALDIDLCKIAQACGYEIVLSVSDNEGLCEALKKIQQIRGP
                     ILLEVKVRKGARKNLGRPANTPIQNKEAFMLFLDNL"
     CDS             6963..8057
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Fe-ADH"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg15_8"
                     /sec_met_domain="Fe-ADH (E-value: 7.8e-76, bitscore: 247.2,
                     seeds: 218, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEQRFFLGESSIKELIPILQEYTPDHIFLVRGKKSYEKCGAQAVL
                     ESVFVKLACGVTEFFDFEANPKIEDMEKGLSLLKEYPTNMIIAIGGGSVLDMAKLLRFF
                     YSYSGNPTGGEFEKKRQLIPLITLPTTAGTGSEATHFSVLYKDKVKYSVEHDAILPDVA
                     IVYPKFTYGNSKYLTSCTGFDALAQAIEAYWNVNATEESDKYAAKAIKLLWPNLPLVVN
                     SPTAEARNKVSEGSYWAGRAINITKTTAPHAFSYSFTTDYGYPHGHAVALTFPFFMRYN
                     IGNSDILLNPNINPYLYKIKMQKMLQWLNMECVDVAIAMKEYIRNLGLDIDISQIDISK
                     IVSSVNIQRLINNPYKVEAICLEE"
     CDS             8042..8758
                     /locus_tag="ctg15_9"
                     /transl_table=11
                     /translation="MSRGISEKEQRWLLVLSQVIEVLDKNNLLYFIDTGTLLGAVRDKM
                     FIPWDNDIDLAVIGGDTSTTNLLKVSKLLYALGFYVTLTKKEIAICSRDGVNLGIKLYK
                     KQASHYEACMGKVYRHRLFYSINLYLSGEVLYKKGFSFKYKAISLFVKFSQVFKLKLSC
                     IWQMKLLFWAKYDEKTVIIPFEFVDAIIDYNFYNIIVKVPNKPERYLAYRYGENWKVPK
                     QDYDYMIDDKSIINID"
     CDS             9141..10028
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg15_10"
                     /sec_met_domain="Glycos_transf_1 (E-value: 1.8e-12,
                     bitscore: 39.3, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDNLVTRHNIGGVLTIFGPARWKPKCKHLCGFARSHLVLNDSPYF
                     QRMSWKELMIEKARNLYLYYYFKRGVTAFWTENPYISEKVKHLFPTIKAYTVSNYYNQV
                     FDDENTQIEHALPSFDGCTLLSINAPYPHKNLAIAIEVARYLKKHYSEFSFRFVFTVTE
                     NDLPPIPSYLREHFCLIDKVSVAECPSLYRQADIMFQPTLLECFTATYVEAMRMQVPIL
                     TTDLPFAHGLCGNAAVYYSPLDAEDCAKSIYKMAVDTSFQDYLVGEGIKQLKSFDNYNR
                     RAEKLIDLISNLDD"
     CDS             10043..12289
                     /NRPS_PKS="Domain: NAD_binding_4 (467-556). E-value:
                     6.7e-09. Score: 27.0. Matches aSDomain:
                     nrpspksdomains_ctg15_11_NAD_binding_4.1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score: 74.2;
                     E-value: 2e-22)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg15_11"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 1.8e-107,
                     bitscore: 350.8, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="RmlD_sub_bind (E-value: 2.5e-07, bitscore:
                     22.0, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVFKDKVLLITGGTGSFGNAVLRRFLDSDIKEIRIFSRDEKKQD
                     DMRHHLQNPKVKFYIGNVRDRQSVDGAMMGVDYVFAAAALKQVPSCEFFPLQAVQTNVI
                     GTDNVLESAIAHGVKNVVVLSTDKAAYPINAMGISKAMMEKVAIAKGRALGEEAGTTIC
                     CTRYGNVMASRGSVIPLWVEQIKNGRPITVTDPNMTRFMMTLDDAVDLVIYAFEHGHNG
                     DLFVQKAPAATLDVLAQALKETYAQVDPKYGETEVKTIGTRHGEKLYETLVTREEMAKA
                     VDMGDYYRIPCDTRDLNYDKFFTEGNEDIARIEDYHSHNTARLDVEGMKKLLLKLRFIR
                     EDLGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVGRNLVSQLHNIRDGKARNYGI
                     ASEELAIYEYDMDSDPAELDVYCRQADFVFNLAGVNRPQDASEFMKGNFGFASTLLDTL
                     RKHGNTCPVMISSSTQAALDNPYGESKRAGEQLLFEYSRETGAKVLVYRFPNVFGKWCR
                     PNYNSAVATFCHNIAHDQPIRVNDPSVVMNLVYIDDVVDELVSAMGGREHRNGDYCEVP
                     VVHTITLGGIVELIRSFRQMPGTLSVPDLSDAFTKKLYSTYLSYLPEERFSYPLKMNVD
                     DRGSFTEIIRTSDRGQFSVNISKPGVTKGQHWHHTKNEKFVVVSGHGLIQLRKIGTEEV
                     VSFEVNGGRMEVVEMIPGYTHNIVNLSETEDLVTFMWCNECFDPARPDTYFEKV"
     CDS_motif       10070..10126
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg15_11_0001"
                     /evalue="4.10E-03"
                     /label="PKSI-KR_m1"
                     /locus_tag="ctg15_11"
                     /protein_end="28"
                     /protein_start="9"
                     /score="9.8"
                     /tool="antismash"
                     /translation="LITGGTGSFGNAVLRRFLD"
     aSDomain        11444..11710
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg15_11_NAD_binding_4.1"
                     /evalue="6.70E-09"
                     /label="ctg15_11_NAD_binding_4.1"
                     /locus_tag="ctg15_11"
                     /protein_end="556"
                     /protein_start="467"
                     /score="27.0"
                     /tool="antismash"
                     /translation="MISSSTQAALDNPYGESKRAGEQLLFEYSRETGAKVLVYRFPNVF
                     GKWCRPNYNSAVATFCHNIAHDQPIRVNDPSVVMNLVYIDDVVD"
ORIGIN
        1 ctttaaaagg aaaggataaa gttcttcata tttgtaagtt actatcggca gatacttatt
       61 ataatgctat aggtggaaaa caaatctata ataaggaaga ctttatgcaa aatggtatta
      121 atttgaaatt tgtagatacg ataataaaaa ggtatcccca gcaatctaaa gaatttgttc
      181 catatttatc aataatagat gtactaatga ataattctaa agaagaaact tgtatgttat
      241 tagattcatt tagtttacaa tgatgtcaat aggtggctat tttgaactgg aaatacgtga
      301 aggagaacat tttcatcaag gtgcaataaa actgagtact gctcgtcaat gctttgagta
      361 tatattaaaa gctagacggt atacaaaagt gtatattcca tattacactt gtgatgtgat
      421 aatagaacct ctcatgaaat taaatgtaaa gtatgaatat tatcatataa atagtttact
      481 ggatcctgtg tttgataaaa aaatagaaag ctcagaggct tttctatata ctaattattt
      541 cggcttaaaa caagatactg ttctttattt atctcggttt tatcctaact taatagttga
      601 taatgcacaa gctttttttg caaaaccaat tgaaggggta gatacatttt attctgcccg
      661 taaatttttt ggagttccag atggcgctta tttgtacaca actgcgttac tggatattga
      721 tttcccgatt tataatccta ttgataatat taactacttg atagggcgta taactgactc
      781 tgcagagggg tattataaag attttcaaga atctgaagaa aggatgtcaa atcaagagat
      841 acatagaatg tctgtattgt ctgataaaat tttgtcttct ttggattacg tctcaataat
      901 tgaaaggcgt cgttttaatt atcatcttct ctacagtgca ttaactgcat ttaataaatt
      961 tgattttagt atttctgaaa atcaaaatgt cccgttgata tatccttttt atacgagcaa
     1021 agctgggctt cgccaacaat taatattaga gcacgtatat gtacctcaat attggcaaaa
     1081 cgtagtgaat aagatagacg ctaaatgtct ggaatactca atcgcgaata atatgattgc
     1141 attgcctata gatcagagat atgatgaaaa aacaatgaat atattaattg aaaaaatatc
     1201 taacttaaat aaccatttat catgaaaaag aaaattatta tttttggagc aaccggtaat
     1261 acagggtctt atttgttaga atacgcatta aatttcttta ataaggaaga gtatgaaata
     1321 atagcttcag gacgtcgcga aactactttt ttttccaaaa ggggaatacc atattattct
     1381 gtggacatta cgaaggaaga agattttgat aagcttccaa tggaaaatat acatgcagtt
     1441 atgctactct ctgctcaaat accttcttat atggatactt attgccccat aaagtattta
     1501 gattctattg taataggagg atttaatgta ttagagtatt gccgcaaatg tcatgtagat
     1561 aggataattt atactcaaac cgtttttgat atttcattgt attctcatga tactatttta
     1621 aaacccgata tgaaaccaaa ttttagctat acgggtgatc atgctatata tgtaatttcc
     1681 aaaaatactg ctttagactt gatagagcat tattatcaag aatacggttt gaaaaaattc
     1741 atttttaggc ttccaacaat atatagctat agcccttatc cgtactactt tcctaatgga
     1801 gtcaagacga agcgacctat ttaccaaatg attgaaaatg ctcagaaagg actacctata
     1861 gagatatggg gagatcctca ttatgcaaag gatatggttc atgttaacga ttttagtcaa
     1921 atgttgtgta aagctgttga ggtggaacaa gaaagtggct tttataatgt gggtacagga
     1981 atgccaatta cattagaaga acaaattcgc actattatta gtgttttttc gcctaaggag
     2041 tcaccatctg aagttatata tagaccagac aaagtttgtg gtggtgggta cataatgaac
     2101 attgaaaatg caaagaaaga attaggatat gtgcctcaat atgattgtcg taagttgttt
     2161 gaggattata aaagtgaaat ggaaataaag cgttttgctg aattaagatt aaaataaatg
     2221 aacggttcaa taacagttag catatgttgc ttgacgtata atcatgaaaa atatataagg
     2281 cagtgtttag atggccttat aatgcaaaaa acagaatttg attttgaaat tcttgttcat
     2341 gatgatgcgt cttctgatgc tactcagagt attattaatg aatatgttgt ggcatatcct
     2401 aatcttatta aaccaattct tcagattcga aaccagtatt ctcaaggtat ttctccgtta
     2461 cgtcaaatat tatttccttt agtaaaaggt aaatatgttg cgctttgtga gggggatgat
     2521 tattggactg atccgttgaa gttacaaaaa caagtaaatg caatggagag tgatcattcg
     2581 ttagcaggtt gtgcgcatca atctttagtt ttttatgaga atggaactcc atcccatatg
     2641 tttcatcatg tgaaaaaaca gattttaaaa actaaagact tattggatgt aagacttttt
     2701 catacagcgt ctttcctttt taaaaaagaa gtacttgatt atattaaaga tttcaatagt
     2761 agtttatatg ctggagatcg ttttctgttt ttggcttgtt cgttgaaagg aaatatttta
     2821 tatatgccag atgtaatgtg ttgttatagg aagaatgcaa tgggagttac ttctagagtt
     2881 tcttattcat tacatgtatt agacctaaaa gttcctgaat attttcataa taaagatttg
     2941 aaatttcctt attacagata tttggcattt gttcatagag ctatattttc agaatcactt
     3001 gacatttcct taaagaaata tatatatcat tttactttat ttgtgaaata ttctttttct
     3061 tattttccta agaatctgaa agatatctat aaggctttat atgtttcttt ctggtggttg
     3121 acaaagggac aaaagtatag aatcaaatga ttgctataat ttttcttatt ctattcttaa
     3181 ttcagatttt gatatctaat aggcttataa ttgattcttt ttcaagaaaa ataagtttca
     3241 tatatttatt gtggtgggga actgctattg ttatttctac ttttgatcca gctgaactat
     3301 attctgtgtc aatgaaaaca tatatgttat tattgatcaa tgtttgggcg tttaattggg
     3361 gatttctttg ttttggaaaa ataaaaaaaa tacgattgaa ggttacaaca gataatagtt
     3421 tttataatct ggttattggc aataataaga tgatattact tcttatgttg gcatgtattg
     3481 ttacttatta ttacgcaatg aagtttgctc tagttattga actatatgga gcgggcgaag
     3541 gtcggatgat gagatttgag gctggtgaac tttttacgag taatttagaa gtatatctat
     3601 atgattttct aataaaacct tttacacagt ttgttatgct aattgtagcg tgtatgctac
     3661 tttctaagat taaacgtaga attctgttgc tatcggtgct ttttctgttt ttatacgcat
     3721 ctgttggtaa tgggcgtggg ggattgattg tttttctatt ttattgtttt atcatttatt
     3781 atttaagaag agaattgtac tatatcaatt tgttagccag caaaaggaaa aaatggaatt
     3841 ttggtgtagt ttcttttgta tgcttgttcg ttggcgttgg aataatagtt gtggttattt
     3901 catatatgac agctcaaaga atgggatttg atacatttag tgctgaggtc gtaaaaatag
     3961 gttttgaaga actaaaaagt caggccgtat ggtatttttt aggtccattt agagctttag
     4021 actattcatt tagagctaat tatgaatcga taaccgggtt ttggtatgga agggcaacaa
     4081 tgggtggaat tgataattta ttaaaacttg taatccatta tttaggagga agttatacca
     4141 ttgctcatga tgaaataggt catcttttgc aaaattcaaa aatttcagtt gggaatggaa
     4201 tcatctttaa ttatgcatac actaatataa tatttcatta tttggatttt ggttacgttg
     4261 gcgttctttt attcccattt ttatatggct ttatcgtacg atatagtatt tataaattta
     4321 ctcaaaaacc aacattactg actcttggta ttatgatata tttggtctat atatcaatgt
     4381 tcaatatttt ttcgtgggaa tttcagaagc cttcctcaat tatttatgtt ttgttgttgc
     4441 tattttttca atatgtttat tctcataaat tcgtaatatt aaaattaaat ggaaaaaaaa
     4501 aaggtttatg tcggcatgag tgctgatatt attcatccag gtcatttgaa tatcattcat
     4561 gaggcttcaa aattggggca tgtaacggtt ggtgtattaa cagattcagc tattgccagt
     4621 tacaagcgtt tgccttactt gaattttgat cagcgctcat tgatagttct gaatttgaag
     4681 ggtgttgatg aagtaatacc tcaaactact cttgattatg ttccgaattt ggaattgatt
     4741 aggcctgact ttgtagtaca tggtgatgac tggaaagagg gtattcaaca agaaactcgc
     4801 cagcgtgtga ttgatactat ctctaaatgg ggtggaaaag tgatagatgt tccttatacg
     4861 aaaggtattt cttccacttt attgaatact aggttgaagg aaataggaac cactcccgaa
     4921 attcgtttga agcgacttaa acgcttaatt gctgctaaaa gtattgttcg tatctgtgaa
     4981 tctcatagtg ggttaacagg tttgataata gaaaatacca gtgtagaagt caacggagtt
     5041 aagcgtgaat ttgatgggat gtggtccagt agtcttactg attcgacttc taaaggtaag
     5101 cctgatattg aagccgttga cttgacaact cgtcttcatg atttgaatga tgctttggag
     5161 tgtactacca aaccagtaat ttttgatggt gatacaggtg gtaaaataga acattttgta
     5221 tttactgttc gcacgctgga acgtttaggg atatcagctg ttattatcga ggataaaata
     5281 ggtctgaaga agaactcgct ttttggaaca gacgctgttc agacccagga tacaattgaa
     5341 gggttttgtg ataaaattca agcgggtaaa cgtgctcaga taacggatga ttttatgata
     5401 atagctcgta ttgaaagttt tattgctgga aaaggtttag atgatgcttt aggacgtgca
     5461 ttagcatata ttaaagcagg tgcggatggg attatgatcc atagcaaaga taagtctggt
     5521 gaggatataa agaacttctg tcttgcttta cgcaaaatac ataaatccat tccgatagtg
     5581 gttgttccaa ctacttataa ccatatatca gaagatgaat tggcttcttg gggagtaaat
     5641 gttgtaatat atgcaaatca tatgcttaga agtgcatatc ccgctatgct taatacggcg
     5701 aagtccattc ttgccaatgc acgctcttat gaagccaatg acttatgcat gcctattaaa
     5761 gaaattctgg aattgattcc agggactaag taaatagact ctttaaaatt aaattcttat
     5821 tattatgatt tctcctaata tttttgtaga ctctcttggc gctatgcaga tggatttttt
     5881 tactggagtt cccgattctt tattgaagaa tatatgtgca tatttaacag atcaccttgc
     5941 ttctgaacat aacattattg ccgctaatga aggtgctgct ataggtttgg ctacaggata
     6001 ttatttggct acgaggaaaa ttccagttgt ttatatgcag aattcaggtc ttggaaatgc
     6061 agtaaatcct ttaatgtcat tgacggataa agaagtttat aatattccat tgttattgct
     6121 aattggttgg cgtggtgaac ctggtgtgaa agatgagcca caacatatta agcagggaaa
     6181 agtcacactt ccattacttg aggcaatggg tatcagatat gctgttttat ctcaagatga
     6241 aaagaagttt atatctcaac ttaatgttgc aaaatcttac atggatgaga caagagagcc
     6301 ttttgctttt gtaattcaga aagacacttt cttagagtat agttctgaaa attgtgaaga
     6361 tgaacggttg tctttgaaac gcgaagctgc tattcatttc atagcagctt cattaacaga
     6421 aaaagatatt ataatatcga caacaggtat gatatcacgt gaattatttg aatatcgtga
     6481 atctacttct ttagaacatg agagagattt tttgactgta ggttctatgg ggcacgcgtc
     6541 gcagatagca ttgggaatag ctcttaaaaa gagagactgt aaagtttatt gctttgatgg
     6601 agatggttcc actttgatgc atatgggttc gttagcaatt gtcggttctt tacatccgga
     6661 aaactatgta cacgtagtct ttaataatgg cgctcacgat tctgtggggg gacaaccaac
     6721 agtcgcttta gatattgatt tatgtaaaat tgctcaagct tgtggttatg aaatagtatt
     6781 gtctgtttct gataacgaag gtttatgtga agctcttaaa aagatacagc aaatacgcgg
     6841 ccctattttg ttggaagtaa aggttcgaaa gggagctcgt aagaatttgg gaagaccagc
     6901 caacactcct atacaaaata aagaggcatt catgcttttc ttagataatt tatagagtaa
     6961 ttatggaaca gcgttttttt ttaggagagt cttctataaa agaattgatt cctatcttgc
     7021 aagaatatac tcctgatcat atatttcttg taagaggaaa aaagtcttat gagaaatgtg
     7081 gtgcacaggc tgtgttagaa tctgtttttg ttaaattagc ttgtggcgtg acggaattct
     7141 ttgattttga agcaaatcct aaaattgaag atatggaaaa aggactttca ttattgaaag
     7201 aatatccgac aaatatgatt attgctatag gtggaggtag tgtattagat atggcaaaat
     7261 tgcttcgttt tttttattcc tattctggta atcctacggg gggggagttt gaaaagaagc
     7321 gacaattaat tccattaata acacttccta ctacagctgg tacaggtagt gaagcaactc
     7381 atttttctgt tttatataaa gataaagtaa aatattcagt agaacatgat gctattcttc
     7441 cggatgtagc gattgtatat ccaaaattta cttatggaaa ttcaaaatat ctaacatctt
     7501 gtacaggctt tgatgctcta gcgcaggcga tagaagctta ttggaatgtg aatgctacgg
     7561 aagagtcgga taaatatgcg gcgaaagcaa ttaaactctt atggcctaat ttgccattgg
     7621 ttgtaaattc tcctacagcg gaggctagga ataaggtttc ggaaggctct tattgggctg
     7681 gtcgtgcaat taatataact aagactacag ctccacatgc tttttcgtat tcatttacaa
     7741 ctgattatgg atatccgcat ggacatgcag tagcattaac atttcccttt tttatgagat
     7801 ataatattgg aaattcggat atcttattga atcctaatat aaatccttac ttatataaga
     7861 ttaaaatgca aaaaatgtta cagtggttaa atatggaatg cgtagatgtt gcaatcgcta
     7921 tgaaagagta tataagaaac ttgggattag atattgacat atctcaaatt gacatatcga
     7981 aaatcgtatc ttctgttaat attcaaagat taataaacaa tccatataaa gttgaagcga
     8041 tatgtctaga ggaataagtg aaaaggagca acgatggttg ttagtattat cacaagttat
     8101 tgaagttctt gataaaaata atttattata ttttattgat actggtactc ttttaggagc
     8161 tgttagagat aaaatgttta ttccttggga taatgatatt gatttagcag ttattggagg
     8221 agatacatct actactaatt tgcttaaagt gtcaaaattg ttatatgctt taggctttta
     8281 cgtcactttg acaaagaaag aaattgctat ctgttctaga gacggggtaa atttaggaat
     8341 taaattatat aaaaagcaag caagtcatta tgaagcatgt atgggaaaag tttatagaca
     8401 tagactattt tattctataa atttatacct ctctggagag gtcctgtata aaaaaggctt
     8461 ttcttttaag tataaagcta tctcactctt tgtgaagttt agtcaagtct ttaagttaaa
     8521 attgtcttgt atttggcaaa tgaagttatt gttttgggct aagtatgatg aaaagacggt
     8581 gattattcct tttgagtttg tagatgcaat tattgattat aatttttata atataattgt
     8641 gaaagtgcca aataagccag aaagatatct agcatataga tatggtgaaa attggaaggt
     8701 tcccaaacaa gactatgatt atatgattga tgacaagtct attataaata ttgattaaaa
     8761 aaatatgaaa acaaatttca acataaggag tattattgta attaataaaa ttataatcaa
     8821 ctttttcttt agacaatata gatggtattt ataaatagca ctttcattta tacaccaaaa
     8881 ttgggcactt atatattata ttctcggaac aaatgaacat tctaataaat tgctccaatc
     8941 tcaagaaagg tggagggctt caagtcgctg attcggtttg ttcttgtttg aatacaattc
     9001 cccaacatga ttttttttta gtattatcct catgtatgga tgatactgct gatagggtat
     9061 cggaatacaa aaatgtcact gtagtgcgtc atgatatacg aaacagttgg tatacattgt
     9121 tttggggacg tgataaggct atggataatt tggtaaccag acacaatatt ggtggagttc
     9181 ttaccatatt cggacctgca agatggaagc cgaaatgtaa gcatctatgt ggatttgcca
     9241 gatcccatct cgttttgaac gattctccct attttcaacg tatgtcttgg aaggaactaa
     9301 tgatagaaaa ggcgagaaat ctttatttat attattactt caaacgtggt gtgactgctt
     9361 tttggactga gaatccttat atttcagaga aagtaaagca cttgttccct accataaaag
     9421 cctatactgt atcgaattat tataatcagg ttttcgatga tgaaaatact caaatagaac
     9481 atgccttacc gtcttttgac ggttgtactt tactgtccat aaatgctcct tatcctcata
     9541 agaatttagc aattgctatc gaagtcgctc gatatcttaa aaaacattat tccgaattta
     9601 gttttcgatt cgtttttacg gtgacagaga atgacttgcc acctattcct tcttatttgc
     9661 gagagcattt ctgtctgatt gataaagtta gtgtagcaga atgcccttcc ttatatcgtc
     9721 aggcagatat aatgtttcaa ccaactttac ttgaatgttt cactgccaca tatgtcgaag
     9781 cgatgcgaat gcaagtgcca attttaacta cagatttgcc atttgctcat ggattatgtg
     9841 gaaatgcagc tgtgtactat tcgccgttag atgcagaaga ctgtgccaaa tcaatttata
     9901 aaatggctgt agacacatcc tttcaagatt atctggtcgg agaaggaata aaacaactta
     9961 agtccttcga taattataat cggagagcag aaaaattaat cgatttgata tcgaatttgg
    10021 atgattaata catattatat ttatgtcagt atttaaagac aaagtcctcc ttataaccgg
    10081 aggtaccggc agttttggta acgctgtatt gcgtcgtttc cttgattctg atatcaagga
    10141 gattcgtatt tttagtcgtg acgagaaaaa gcaggatgac atgcgtcatc acctgcaaaa
    10201 tcccaaagta aaattttaca ttggtaatgt ccgtgaccgc cagtctgtag acggtgccat
    10261 gatgggagtg gattatgtat ttgccgctgc cgccttgaag caggttccca gttgcgagtt
    10321 cttcccgttg caggctgtgc agaccaatgt gatcggtacg gacaatgtac tggaatctgc
    10381 catagcgcat ggtgtaaaga atgtggtggt gctttctacc gacaaggccg cctatcccat
    10441 caatgccatg ggtatcagca aggccatgat ggagaaggtt gccatagcca agggccgtgc
    10501 gttgggtgag gaagccggta cgaccatctg ctgtacccgt tacggcaatg tgatggcaag
    10561 ccgcggttct gtaattccct tgtgggtgga gcagataaag aacggcaggc ccattacggt
    10621 aacggacccc aacatgaccc gcttcatgat gacgctggac gatgccgtgg atctggtaat
    10681 ctatgcgttt gagcacggcc ataacggtga cctgttcgtg cagaaggctc ctgccgccac
    10741 cttggatgta ctggcgcagg ccttgaagga aacatacgcg caggttgatc cgaaatacgg
    10801 tgaaacggag gtgaagacca tcggcacgcg ccacggcgag aaactgtatg agacactggt
    10861 aacccgcgaa gagatggcaa aggctgtcga catgggcgac tactaccgca tcccttgcga
    10921 cacccgtgac ctgaactatg acaagttctt cacggaaggc aatgaggata ttgcccgtat
    10981 agaggactat cacagccata acaccgcccg cctggatgtg gagggcatga aaaagcttct
    11041 tttgaaactc cgcttcatcc gcgaagacct cggnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11161 nnnnnnnnnn nnngtcggcc ggaaccttgt atcccagctg cacaacatcc gtgacgggaa
    11221 agcccgcaat tacggcattg caagtgagga acttgccatt tacgagtatg acatggacag
    11281 tgaccctgcc gaactggatg tctattgcag gcaggctgac tttgtcttca atctggcggg
    11341 cgtgaaccgt ccccaggatg cgtctgagtt tatgaaaggg aacttcggct tcgcctccac
    11401 gctgctcgac acattgagga aacacggcaa tacctgtccg gtaatgatct cctcttccac
    11461 gcaggccgca ctcgacaacc cttacggtga atcgaagcgg gcaggggagc aactgttgtt
    11521 cgaatactcc agggaaacgg gggcgaaggt actggtgtac cgcttcccga atgttttcgg
    11581 caaatggtgc cgccccaact ataacagcgc ggtggccact ttctgccata acatcgccca
    11641 tgaccagccc atccgggtga acgacccttc ggtggtgatg aacctggttt acatagacga
    11701 tgtggtggac gagcttgtct ctgccatggg cggcagggaa caccgcaacg gtgattattg
    11761 cgaagtgccc gtagtacata ccattacctt gggcggcatt gtggaactga taaggtcttt
    11821 cagacaaatg cccggtacct tgtccgtccc ggatttgagc gatgccttta caaagaaact
    11881 ctattccaca tacctctcat acctgcctga agaacggttc agctatcccc tgaaaatgaa
    11941 tgtggacgac cgcggcagtt ttacggagat tatccgtact tccgaccgcg ggcagttctc
    12001 ggtgaacatc tccaagccgg gcgttacgaa aggccagcac tggcaccata ccaagaacga
    12061 gaagtttgtg gtggtgagcg ggcacggtct gatacagctc cgcaagatag gtacggagga
    12121 ggttgtcagc ttcgaggtaa acggcgggag gatggaggtc gtggagatga ttcccggcta
    12181 tacgcacaac attgtcaacc tttcggaaac ggaagacctg gtgaccttca tgtggtgcaa
    12241 cgagtgtttc gacccggcac gtccggacac ttattttgaa aaagtctgaa acgagttaaa
    12301 gaatatagga acagatggat ataaaattgg attattcgca agtcaggttt caggagaacg
    12361 gcagactgaa acttctgatt atagtgggca cccgtccgga aatcatccgc ctggcggctg
    12421 tcatcaacaa atgccgcagt tattttgact gtcttctggc ccacaccggc cagaattacg
    12481 actataactt gaacggtgtc ttcttccgtg acctgaagct gaaagccccc gatgtgtata
    12541 tggatgccgt cggcgatgac ctcggtgcca cgatggggaa tattatcaac gccagctaca
    12601 agctgatggt gcaggtgaaa cctgatgccg tgctggtgtt gggagacacg aactcctgcc
    12661 tgagcgtgat aggcgcgaaa cgtctgcata tccccatctt ccatatggag gcaggcaacc
    12721 gctgctttga cgagtgcttg cctgaggaga ccaaccgccg tattgtggac gtcatttccg
    12781 atgtgaacct ctgctattcg gagcatgccc gcagatacct gaacgcttcg ggcgtggcga
    12841 aagaacgtac atacgtaacc ggttcgccca tggcggaagt cctgcatgag aatttgtccg
    12901 aaatagagtc cagcgatatt caccagagat tgcatctcca gaaaggtaaa tacatcctgc
    12961 tttccgcgca ccgggaagag aacattgaca cggagaagaa cttcctgtcc cttttcagcg
    13021 cggttaacgc tatggccgag aaatatgaca tgcccat
//
