LOCUS       c00261_gnlPro..         7469 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KGNEPMAM_261.
ACCESSION   c00261_gnlPro..
VERSION     c00261_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 08:59:09
            Original ID  :: gnl|Prokka|KGNEPMAM_261
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7469
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..2097
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg261_1"
                     /sec_met_domain="Gly_radical (E-value: 3.2e-46, bitscore:
                     145.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.7e-268, bitscore:
                     880.9, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LRLWDELKVLLKREIDNGGVLDADEEVVSSITSHKPGYIDKELEV
                     VVGLQTDAPLKRALMPFGGLRMAQQALESYGFKMCEKTADIFKKIRKTHNEGVFDAYTS
                     DIRAARSAGIITGLPDAYGRGRIIGDYRRVALYGTDKLIAERRKDLKNREHSPLTDELI
                     RLREEMSEQIRALEELAQLGASYGCDLTRPAANAREAVQWTYLGYLAAVKEQNGAAMSL
                     GRVSTFFDIYFTRDLEQGLITEEEVQEIIDQFVMKLRIVRFIRTPDYNNLFSGDPTWVT
                     ESIGGMGEDGRTLVTRSSFRMLQTLYNLGPAPEPNLTVLWSRNLPEAFKSFCAKVSIET
                     SSVQYENDDLMRPHWGDDYGIACCVSAMRIGKQMQFFGARANLAKCLLYALNGGVDELK
                     GKQVAPPSPRYTEEILNYDEVMALYDKMQDWLAKTYIDALNIIHYMHDKYCYERIEMAL
                     HDPEILRTMATGIAGLSVAADSLSAIKYATVKAIRNEEGLIVDFKTEGEFPCYGNNDPR
                     VDDIACSLVSNFMEKLRRLHTYRNSLPTQSILTITSNVVYGKKTGNTPDGRRAGEPFAP
                     GANPMHGRDRNGAVASMLSVAKLSYDDSLDGISYTFSIVPQALGKEERERRVKLVSLLD
                     AYFAATGHHINVNVLERETLLDAMDHPEKYPQLTIRVSGYAVNFIKLTREQQQEVINRT
                     FHTR"
     protocluster    1..7469
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:2893]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..2893
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7469
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7469
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2114..2893
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 53.6;
                     E-value: 9.1e-17)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg261_2"
                     /sec_met_domain="Radical_SAM (E-value: 5.5e-32, bitscore:
                     100.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQVFSQNHDPDGESSVTGLVHSVESCGTVDGPGIRFVLFLSGCS
                     LRCRYCHNPDTSYVRRGRLRSADDVLKEIARYRDFLQAAGGGVTLSGGDPLFQPDFAGA
                     VLKGCRKLGLHTCLDTSGHLGVNAGEEMLADTDLVLLDIKAWNPKRYRALTGGELRPTL
                     EFAERLASLRKPVWLRYVLVPGVTDNLEEIEELSRHAALLGNVERVDVLPFHQLGRFKW
                     DELKLDYTLKDVPEASAELAEQVRGIFRRGGFPDCVH"
     CDS             complement(3141..5066)
                     /locus_tag="ctg261_3"
                     /transl_table=11
                     /translation="MTDSSSPDSSPASRNTFAHHLGMKGFLLMEGLLKLVGMKTLYRLG
                     RAAGAVAWHLLPQRRNIVERNLRIVLDPALRGKELQKLSRENFKRTIANFLCSAKTATL
                     TDEQLKHCVTVGGHEQFAAPVLEGRGQVCAIAHSGNWEALARIRAFYPEVERYGSMYRQ
                     FDNPLMEEYVYQRRTERGTQMFSKEGGIKAPMKMLKEGGALGVLSDQFVWEGVYVPFFG
                     KVTGTTPLPALLRKRAGADMVAIAVRTDVPGHWIADMGNVVDFSRSDGSLAGDTIEVNR
                     GLETLIRESVLDVFWMHHRWKSVDRFAPQDKKTAALLENMELKPYRILVAVPRALDEAL
                     ATVPLIRALKTVRCDMQVNIVCPSAQLGIWKTVPEVTHVLPHDSLKQLREALVADEFYN
                     DGPLDLGVMLDGDMETLKALEPYGPMMFSGLDTHPGARKYKFRVKAPVLRAAPPAHRVQ
                     LYLQLGVLHGLDVATPSLFPVKKAAPAEDAPILIAPFSRLGSASEWSREQWRELVSLLP
                     GRTVLAALEEDRERAASLAEHLNVELAVGAPEALFPVMDVAAAAVAVDGDFPSLCSFRG
                     LPVVTLFSTRLPDVYRPMGTFNRSLYSHQCCSPCFWEECDRDVPCNRHIAVREVLEALR
                     EISGKE"
     CDS             5216..5518
                     /locus_tag="ctg261_4"
                     /transl_table=11
                     /translation="MLYCIFLIKASLRRRHLTFGKEMTLQFLTNRYIPSPEPNTKPELY
                     TMKVLSSLASMKRRHADCQIVKRKGTLYVICKSNPKFKARQGATAGTRLSKKGVK"
     CDS             5605..6744
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_1"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg261_5"
                     /sec_met_domain="Abhydrolase_1 (E-value: 1.9e-30, bitscore:
                     95.4, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQLPAVQTKFLDLPNSFSLRNGATLDKVRVAYEQYGTLTPNKDNA
                     ILLFHALSGSQHAYGYNPEVPGIGSLWKPENHEGWWNSIIGPGKPLDTNRFCIICANYL
                     GGCYGTTGPATPCPADGQPYGSRFPHVEAADQARLQALLLDSLGIERVHLMGPSVGGLI
                     ALSFACQFPERVRSFISIGSGYRASIEHRLSLFEQILAIELDPDFQGGDYYRGPVPKKG
                     LAFARIIGHKSFVYQEGLEQRARKEVGGNYGLLTWMTPTRSTQSYMLHQGTKFAERFDA
                     NAYIRIADMWAEFDIRDHTPDGTFQTALEGFRRAGIPALIFSIDTDCCFRPAEQQDFAA
                     QLEAAHIPTEFHTIASTKGHDSFLLEPELYAEPIRRALG"
     CDS             6835..7020
                     /locus_tag="ctg261_6"
                     /transl_table=11
                     /translation="MLLGNAWKPTDNHHRAFHDGCHIHCLSGKKDMSYYFIVTISLTGD
                     AHTPYDAYVREIKAHC"
     CDS             complement(6995..7207)
                     /locus_tag="ctg261_7"
                     /transl_table=11
                     /translation="MMAWLSTESSRSDLSCRYSSDAKQHSRLSLEGIRMTITRFVFQPE
                     INGMTSFGPDQVFSAVFFNNGLLSP"
     CDS             complement(7305..7469)
                     /locus_tag="ctg261_8"
                     /transl_table=11
                     /translation="DAKSGSYALAGGVCQENPFDFLRSLPGFLHVGKNGLLASVHAGIN
                     QIAGVSLNQ"
ORIGIN
        1 cttcgtttat gggacgagct gaaagtcctg ctgaaacggg aaatagacaa cggcggcgtg
       61 ctggatgctg atgaagaagt ggtatcctcc atcacatccc acaagcccgg ctacattgac
      121 aaggaactgg aagtggtcgt aggcctccag acggacgccc ccctgaaacg ggccctgatg
      181 ccctttggcg gcttgcgcat ggcccaacag gccctggaat cctacgggtt caagatgtgc
      241 gagaaaaccg cagacatttt caaaaagata cgcaagacgc acaatgaagg cgttttcgac
      301 gcctacactt ccgatatccg ggcggcccgt tccgccggga ttattaccgg ccttccggac
      361 gcctatggcc gcggccgcat catcggggac taccgccggg tggccctgta cggcacggat
      421 aagctgattg ccgaacgccg caaggacctc aaaaaccgtg aacattcccc ccttacggat
      481 gagctgatcc gtctgcgcga agaaatgagc gaacaaatac gcgccctgga agaactcgcc
      541 cagctgggcg cctcctacgg ctgcgacctc actcgccccg ccgcaaacgc ccgcgaagcc
      601 gtgcaatgga cgtacctggg ctacctggcc gccgtgaagg aacagaacgg agcggccatg
      661 tccctgggcc gcgtttccac tttcttcgac atttatttca cccgcgacct ggaacagggg
      721 ctcatcacgg aagaggaagt tcaggaaatc atcgaccagt tcgtgatgaa gctgcgcatc
      781 gtccgtttca tccgcacgcc tgattacaat aacctgttct ccggagaccc cacctgggtg
      841 acggaatcca tcggcggcat gggagaggac gggcgcaccc tcgtcacccg cagttccttc
      901 cgcatgctgc agaccctgta caacctgggg ccggctccgg aaccgaacct gaccgttctg
      961 tggtcccgga acctgccgga agccttcaaa agcttctgcg ccaaggtttc cattgaaacg
     1021 tcctccgtcc aatacgagaa cgacgacctg atgcgcccgc attggggcga cgactacggc
     1081 atcgcatgct gcgtgtccgc catgcgcatc ggcaagcaga tgcagttctt cggagcgcgc
     1141 gccaacctcg ccaagtgcct tctctacgcc ctgaacggcg gcgtggatga actcaagggc
     1201 aagcaggtgg ccccgccctc cccccgctac acggaggaaa ttctcaacta tgatgaggtg
     1261 atggccctgt acgacaagat gcaggactgg ctcgccaaaa cttacattga cgcgctgaac
     1321 atcatccact acatgcacga caaatactgc tatgaacgca ttgaaatggc gctgcatgat
     1381 ccggaaattc tgcgcacgat ggctacggga atcgccgggc tttccgtggc ggcggactcc
     1441 ctgtccgcca tcaagtatgc caccgtaaaa gccatccgca atgaagaagg gctgattgtg
     1501 gacttcaaga cggaagggga attcccctgt tacgggaaca atgacccgcg cgtggacgac
     1561 atagcatgca gcctggtcag caatttcatg gaaaaactgc gcaggctcca cacttaccgc
     1621 aattccctgc ccacccagtc catcctgacg atcacctcca acgtggtgta cggcaaaaag
     1681 acgggcaaca cgccggacgg ccgccgagcc ggggaaccgt tcgcccccgg agccaacccc
     1741 atgcacggaa gggacaggaa cggagccgtg gcctccatgc tctccgtagc caagctgtcc
     1801 tatgacgact ccctggacgg catctcctac accttctcca tcgttcctca ggccctgggc
     1861 aaggaggaac gtgaacgccg cgtcaagctc gtctccctgc tggacgccta ctttgccgct
     1921 acaggtcacc acattaatgt gaacgtactg gaacgggaaa cgcttctcga cgccatggat
     1981 cacccggaaa aatacccgca gcttaccatc cgcgtttccg gctatgccgt gaatttcatc
     2041 aagctgaccc gggaacagca gcaggaggtc atcaaccgca ctttccacac ccgttaaccc
     2101 ggactgcaag cggatgccgc aggttttttc acaaaaccat gatccggacg gagaaagctc
     2161 cgtcaccggt ttggttcatt ccgtggaatc ctgcggcacc gtggacggcc ccggcatccg
     2221 cttcgtcctt ttcctgtccg gctgcagcct gcgctgccgc tactgccata atccggatac
     2281 ctcctacgtg cggaggggcc ggctcaggag cgcggatgat gtcctcaagg aaatagcccg
     2341 ttaccgggat tttttgcagg cggcgggcgg cggcgtcacc ctctccggcg gggatcccct
     2401 cttccagccg gattttgccg gtgccgttct gaaaggctgc cggaaactgg gcctgcacac
     2461 ctgcctggac acctccggcc atctgggcgt gaatgcgggc gaagaaatgc tggcggatac
     2521 ggacctggtc ctgctggata tcaaggcatg gaatccgaaa cggtatcgcg ccctgacagg
     2581 cggggaactg cgccctacac tggagtttgc ggaacgcctg gcctccctcc gcaagcccgt
     2641 gtggctgagg tatgtgctgg tacctggcgt tacggacaat ctggaagaaa ttgaggaact
     2701 ctcccgccat gcggcgcttc tgggcaacgt ggaacgcgtg gacgtgctcc ctttccacca
     2761 gctggggaga ttcaaatggg atgaattgaa gctggactat actttgaagg acgtcccgga
     2821 agcgtccgca gaactggcgg aacaggtccg cggcattttc cggcggggcg gctttccgga
     2881 ttgcgtgcat taaccggatt ttcgcgcttt ggcgccacca accgcagcaa accggacgca
     2941 ggcatccccc tcttccggat gaggggccct cttccctcca ctacagaatc catgcaaaaa
     3001 ggaagacgca tttctggaat tgctccggaa tacggcattt ccgcaattcc tttcgttatt
     3061 ccatcattct ttacggcaac ggcagcattc ccgccgtttt tccgccaacg ccggggggcg
     3121 gcattccgtc ccttcccaga ttattctttc cctgaaattt cccgcaaggc ttccagtact
     3181 tcccgcacgg caatgtggcg gttgcaggga acatcccggt cacattcctc ccagaagcag
     3241 ggagaacagc actggtggct gtaaagggaa cggttgaatg tgcccatagg gcggtaaaca
     3301 tccggaagcc gggtggaaaa cagagtcacc accggaagcc cccggaaaga acagagggac
     3361 ggaaagtcgc catccacagc tactgcagcc gcagcgacat ccatgacggg aaagagagcc
     3421 tccggcgcac ccaccgccag ctccacattc aaatgttccg ccagggaggc ggcacgctcc
     3481 cggtcttctt ccagagccgc cagcaccgtc cgtcccggca gaagggaaac cagctccctc
     3541 cactgctccc tgctccattc actggcgctc cccaggcggg aaaacggagc gattaaaatg
     3601 ggggcgtctt ccgccggggc cgcttttttc acagggaaca aagacggggt ggcgacgtcc
     3661 agcccatgca gcacccccaa ttgcagataa agctgcaccc ggtgcgccgg gggggcggcg
     3721 cgcaggacgg gggcctttac ccggaacttg tacttccggg caccgggatg cgtgtccagc
     3781 ccggaaaaca tcatggggcc atacggttcc agcgctttca gggtttccat atctccatcc
     3841 agcatgacgc ccagatccag cggtccgtca ttgtaaaatt catctgctac caaagcttcc
     3901 cgaagctgct tcagggagtc gtgcggcagc acatgggtaa cttccggcac cgtcttccat
     3961 atccccagct gggcagacgg gcagacgatg ttcacctgca tgtcgcaccg gacggttttc
     4021 aaagctcgaa tcaggggaac agtcgccagg gcttcatcca gcgccctggg aacggctacc
     4081 agaatgcggt acggcttcag ctccatattt tccaggagcg cagccgtttt tttatcctgc
     4141 ggggcaaatc ggtcaacgga tttccagcga tggtgcatcc agaacacatc cagcacggat
     4201 tcccggatca acgtttccag ccccctgttc acttcaatcg tgtcccccgc cagagagccg
     4261 tccgacctgg aaaaatccac cacattcccc atatccgcta tccagtggcc gggaacatcc
     4321 gtgcgcaccg ctatggccac catgtccgcg ccagcccttt ttctcagcag agcgggcagc
     4381 ggagtcgtgc cggtcacctt gccaaaaaag gggacataca ccccctccca tacaaactgg
     4441 tcgctcagca cgcccagggc ccccccttcc ttgagcattt tcatgggagc cttgatgcct
     4501 ccctccttgg agaacatctg agttccacgt tccgtgcgcc gctggtacac gtattcctcc
     4561 atcagcggat tgtcaaactg gcggtacatg gacccgtaac gctccacctc cgggtaaaat
     4621 gcgcgtattc tcgccagcgc ctcccagttg ccggagtggg ctatggcgca aacctgcccg
     4681 cgcccttcca gcacaggcgc ggcaaactgt tcgtgccctc cgaccgtcac gcaatgcttc
     4741 aactgctcat ccgtcagggt ggccgtcttc gcggaacaca aaaaattggc aatggtcctt
     4801 ttgaaatttt cccggcttaa tttctgcagt tcctttccgc gcagggccgg atccaaaacg
     4861 atgcgcagat tcctttccac aatgttccgg cgctggggaa gcagatgcca cgccacggcc
     4921 ccagccgcgc gccccagccg gtacagcgtt ttcatgccca cgagcttcaa cagcccttcc
     4981 atgagcagaa accctttcat tcccaaatga tgggcaaacg tattccggga agccggggag
     5041 gaatcaggag aggaagaatc agtcataagg aaaaccggaa aagatgttat acgaatcccc
     5101 tccggtccac aaacccttta ttcccccggc agagatccct tcccgcattt tattttctcg
     5161 gataaatgac gtcttcccta aatttttccg ccggcgcgga atccaaagcc atcccatgct
     5221 ttactgcatc ttcctcataa aagcatctct gcggagacgg catcttactt ttggcaaaga
     5281 aatgacttta caattcttaa ctaaccggta tattccgtct cccgaaccga acaccaaacc
     5341 ggagttatac accatgaaag ttctttcttc cttagcctct atgaagcgcc gccacgcaga
     5401 ctgccaaatc gtgaagcgca agggcacgct gtatgtgatc tgcaagagca atcccaagtt
     5461 caaggcccgc cagggcgcta cggcaggcac gcgcctttcc aaaaagggcg tcaagtaatc
     5521 cctggcccag cacagatttc aagaagaccg ccgcccgccg ggtgccggtc tttttttgct
     5581 gaccaagccc ctttccgtac atccatgcag cttcctgccg tccaaaccaa attcctggat
     5641 cttcccaact ccttctccct gagaaatgga gctacgctgg acaaggttcg cgtcgcctac
     5701 gaacagtacg gaaccctgac gccgaataaa gacaacgcca tcctgctgtt ccatgctctt
     5761 tccggcagcc agcacgccta cggttacaat ccggaagtcc cgggcatcgg ttccctctgg
     5821 aaaccggaaa accacgaggg ctggtggaac agcatcatcg gccccggcaa accgctggac
     5881 acaaaccgct tctgcatcat ctgcgccaat tacctgggag gctgctacgg caccacaggc
     5941 cccgctaccc cctgccctgc ggacggccaa ccctacggct cccgcttccc gcatgtggag
     6001 gccgcagacc aggcacgtct ccaggccctt ctgctggaca gcctgggcat agaacgcgtt
     6061 catcttatgg gcccctccgt gggcggcctg atcgccctca gcttcgcgtg ccagttcccg
     6121 gaacgggtcc ggagcttcat ctccatcggc tccggctacc gggcttccat tgaacaccgc
     6181 ctgtccctgt ttgaacaaat cctggccatt gagcttgacc cggatttcca aggcggggat
     6241 tactaccggg gaccggtgcc aaaaaaaggg ctggcgttcg cccgtatcat cggccacaaa
     6301 tcatttgttt accaggaagg gctggaacag cgcgccagaa aagaggtggg aggcaactac
     6361 ggcctgctca cgtggatgac ccccacccgc agcacgcaaa gctacatgct tcaccaggga
     6421 accaagttcg cggagcgctt tgacgccaac gcctatatcc gtattgcgga tatgtgggcg
     6481 gagttcgaca tccgcgacca caccccggac ggaacatttc aaaccgccct ggaaggcttc
     6541 cgccgcgcag ggattcccgc gcttatcttt tccattgata cagactgctg tttccgcccg
     6601 gcggaacagc aggatttcgc ggcgcagctt gaagccgccc atattcccac ggagttccat
     6661 accatcgctt ccaccaaggg gcacgattcc ttcctgctgg agccggaact ttatgcggaa
     6721 cccatccgaa gggctctggg gtgagcgtgc ggttctccag aaggttttcc ccctgatcgt
     6781 cccggcagat tgacgcagga tatccctgct ttaatcttcc cgtcaaggct atcaatgctt
     6841 ctggggaatg catggaaacc aaccgacaac catcaccgcg catttcatga cggctgccac
     6901 atccactgct tatcaggaaa aaaggatatg agttattatt ttatcgtcac gatttcatta
     6961 accggagatg cgcatacccc ttatgatgcc tacgtcaggg agataaaagc ccattgttga
     7021 aaaacacggc ggagaatacc tggtcaggac caaatgaggt cattccgttg atttctggct
     7081 ggaagacgaa ccgtgtgatt gtcatccgga ttccctccag ggaaagccta gaatgctgct
     7141 ttgcatcgga agagtaccgg cagctcaaat cgctgcggga ggattccgtg gacagccatg
     7201 ccatcatcgt gaaaggaacg gattatgatg aaactttgtg aaggactgca ccagctcagg
     7261 gttgatttcc gggtgacacc ggaaatcagg aggtttgcgt atacttattg attgagggag
     7321 acgcctgcta tctgattgat tccggcatgg acggaagcga gaaggccgtt ttttcctaca
     7381 tggaggaaac cgggcaaaga ccggaggaaa tcaaagggat tttcctgaca cacgcccccc
     7441 gcgagggcat aagaaccgct cttcgcatc
//
