LOCUS       c00486_gnlPro..         2639 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|GJLHDPKL_486.
ACCESSION   c00486_gnlPro..
VERSION     c00486_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 07:38:53
            Original ID  :: gnl|Prokka|GJLHDPKL_486
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2639
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2639
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:1304]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2..1304
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..2639
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2639
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..577
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg486_1"
                     /sec_met_domain="Gly_radical (E-value: 1.1e-48, bitscore:
                     152.0, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 3.9e-23, bitscore:
                     69.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="PCFGNNDDRVDHLGVDLVYYFSEELKKLPVYKNARPTLSLLTITS
                     NVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIASLSSVAKLRYRDSQDGISNTF
                     SIVPKSLGPTPEERVENLVTMMDGYFTKGAHHLNVNVLNREMLEDAMEHPEKYPQLTIR
                     VSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             579..1304
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 75.5;
                     E-value: 1.7e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg486_2"
                     /sec_met_domain="Radical_SAM (E-value: 5.5e-32, bitscore:
                     99.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCPFRCLYCANPDTIDVKGG
                     TPTTPEEILQMAISQKAFFGKKGGITFSGGEPTLQAEALIPLFKELKANGIHICLDSNG
                     GIWNEKVEELFSLTDLVLLDIKQFNPERHRTLTGRSNEQTLRTAAWLEEHEHPFWLRYV
                     LVPGYSDSEEDIRLLGESLGKYRQIQRVEILPYHRLGVHKYEVMGWDYKLKEVKENTPE
                     QLERAEKLFKEYFTNVITN"
     CDS             complement(1373..2638)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fn3-like"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3_C"
                     /gene_functions="other (smcogs) SMCOG1041:Fibronectin type
                     III-like domain (Score: 88.2; E-value: 1e-27)"
                     /gene_kind="other"
                     /locus_tag="ctg486_3"
                     /sec_met_domain="Fn3-like (E-value: 4.9e-29, bitscore:
                     88.2, seeds: 729, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3_C (E-value: 5.6e-54,
                     bitscore: 170.9, seeds: 116, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="EMVSTCYLTYLKELVQEGKVKESVLDDAVRRILEKKYELGLFEDP
                     FRYCNPERAARILNAPEVRNASLRMAERSVVLLENRESVLPLTAQTRKIALIGGLSKSQ
                     YDMAGAWAATTDRSKVVTLYDALVRKGLEVNYAEGYDMKSNQLTGLQEALQAAEASDVI
                     VVAVGERFGQSGEKASKVNIAIPEGQQKLVSELAKTGKPVIALVMCGRPVIFNEIREHA
                     DAVLCTWWLGSEAGNAICNVLWGEYNPSAKLPMTFPAHVGQIPIYYQNKSTGRPTALQQ
                     TYKASYIDASTEPAYPFGYGLSYTDFGYSDLKLLPGKDAGVHTEVSVKVTNTGKCAGEE
                     VVQLYVQDKVASVTRPIKELKGFRKIHLNAGESKTVAFQITDDALGFYDNEMNYMVEPG
                     DFVFMVGGSSDDVQQITYNLKE"
ORIGIN
        1 cccctgcttc ggcaataatg acgaccgtgt ggaccacctg ggagtagacc tcgtttacta
       61 tttcagcgaa gaactgaaaa agctgcctgt ctacaagaat gcccgtccca ccctttcctt
      121 gctgactatc acctccaacg tgatgtacgg caagaagacc ggtgccactc cggacggacg
      181 cgccaaaggt gttgcctttg ctccgggtgc caacccaatg cacggacgtg acaagaacgg
      241 tgccatcgct tccttgagct ccgtagccaa attgcgctac cgcgactcgc aggacggcat
      301 cagcaatacg ttctccattg ttcctaaatc actgggcccg acaccggaag aacgtgtaga
      361 gaacctggta accatgatgg acggctactt caccaaaggt gcccaccatt tgaacgtaaa
      421 cgtactgaac cgcgaaatgc tggaagatgc catggaacat cctgaaaagt atccgcaact
      481 caccatccgc gtttccggct atgccgtgaa cttcgtgaag ttgagccgcg agcatcagtt
      541 ggaagtcatc agccgttcat tccacgaaag aatgtaatat gataaatgta cactcctacg
      601 aaagtatggg aacattcgac gggccgggtc tccggctcgt cgttttcctg caaggatgtc
      661 cgttccgttg tctgtattgt gccaatccgg atacgattga tgtaaaagga ggcactccca
      721 ccactccgga agagattctg caaatggcta tcagccaaaa ggcattcttc gggaagaaag
      781 gaggtattac tttctccggc ggtgagccta ccctgcaagc agaagcgctg attcctcttt
      841 tcaaggagct gaaggcgaac ggcatccata tctgtctgga cagtaacgga ggcatctgga
      901 atgaaaaggt ggaagagctc tttagcctga ctgacctcgt gctgctggac atcaagcaat
      961 tcaatcccga acgccaccgt acgctgacgg gacgaagcaa cgaacagaca ctccgcaccg
     1021 ctgcctggct cgaagaacat gaacacccct tctggctgcg ttatgtactg gttcccggtt
     1081 acagcgattc ggaagaagac atccgcctgc tgggtgaaag tctcggcaaa taccggcaga
     1141 tacaacgggt ggaaatactt ccttaccacc gtctgggcgt tcacaaatac gaagtcatgg
     1201 gatgggatta caaattgaaa gaagtgaagg aaaatactcc ggaacaactg gaacgggcag
     1261 aaaaactgtt caaagagtat ttcacgaatg tgataaccaa ctaaaacaga aaagcaaggc
     1321 tgtccggata tgattccaga cagccttgct tttcatttat aggacaagaa tcctattctt
     1381 tcaagttata ggtaatctgc tgtacatcat cactactgcc acccaccata aagacgaaat
     1441 caccaggttc taccatataa ttcatctcat tgtcatagaa tcccagtgca tcgtcagtta
     1501 tctgaaaagc tacagttttg ctttcacccg cattcagatg aattttcctg aagcccttca
     1561 actcctttat cgggcgcgta acagacgcta ccttatcctg cacatacaac tggactactt
     1621 cctctcctgc acacttaccg gtattagtca cttttaccga cacctccgta tggacgcctg
     1681 catccttgcc cggcagcagt ttcaaatcag aatatccaaa atccgtatac gaaagcccat
     1741 atccgaaagg ataggcaggc tcagtggaag catcgatata ggatgcctta taagtctgtt
     1801 gcaatgcggt aggacgaccg gtactcttat tctggtaata aatgggaatc tgccctacgt
     1861 gtgcaggaaa tgtcatgggc aatttagccg aaggattata ctctccccac aacacattgc
     1921 atatggcatt gccggcctca ctccccagcc accaagtaca cagtaccgca tcagcatgtt
     1981 cccttatctc attgaagatt accggacgcc cacacatgac aagagcaatg acaggttttc
     2041 cagtctttgc cagttccgac accagcttct gctgcccctc gggtatggct atattcactt
     2101 tggaagcctt ctcgccactc tgaccgaatc tctcgcctac cgccacaaca atgacatccg
     2161 aagcctcagc agcctgcaat gcttcctgca gtccggtcaa ttgattgctc ttcatatcat
     2221 agccttcagc atagttcacc tccagtcctt tccggaccag tgcatcatac aaggtcacca
     2281 ctttgctccg gtccgtagtg gcggcccaag cgcctgccat atcgtattgg gattttgaga
     2341 gtcctcctat caaggctatc ttcctcgtct gtgccgtcaa cggaagcaca gattccctat
     2401 tctccagcaa tacaacggaa cgctcggcca tacggagcga agcattacgg acttcgggcg
     2461 cattgagtat tctggccgct ctttccggat tacagtagcg aaaagggtcc tcaaacaatc
     2521 ccaactcata tttcttctcc aatatacgcc gtactgcatc atcaagcaca ctctccttta
     2581 ctttcccttc ttgaacaagc tccttcagat aagtcaagta gcaggtactt accatctcc
//
