LOCUS       c00048_gnlPro..         3567 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PLHFILAD_48.
ACCESSION   c00048_gnlPro..
VERSION     c00048_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 07:40:46
            Original ID  :: gnl|Prokka|PLHFILAD_48
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3567
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3567
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:3261]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..3261
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..3567
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3567
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             3..473
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: SucD_FumR"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg48_1"
                     /sec_met_domain="SucD_FumR (E-value: 2.3e-12, bitscore:
                     34.6, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="IHVWLTIQLKLENHAAREPYVFKNTIKATLSSRYMIYTGLTVLVF
                     LIYHLYQYTLRVGYDPAQFTTFISDGTVETFDVYKMIVTGFSNVWCSAFYILAVLMLFS
                     HLRHGVQSIFQTVGVDSRKIRPFYNFIAIAYGAIICLGFISVPVSVLLGIIK"
     CDS             495..2453
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 176.1; E-value:
                     4.2e-54)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg48_2"
                     /sec_met_domain="FAD_binding_2 (E-value: 2e-55, bitscore:
                     176.1, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 5.7e-39,
                     bitscore: 120.8, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINFPVSDWMPDAKIPSGPIQDKWSKYKLESKLINPNNKRKYTVL
                     VVGSGLAGASAAATLAELGYNVKCFCYQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRL
                     FYDTVKGGDFRAREANVYRLAEVSSNIIDQCVAQGVPFGREYGGLLDNRSFGGAQLKRT
                     FYARGQTGQQLLLGAYQALEKEVAKGHIEMFSRHEMMDLVLVDGEAKGIVTRNLVTGEI
                     KSYAGDTVLLCTGGYGNVFFLSTNAMGCNVTAAYKAYKRGAFFANPCFTQIHPTCIPVK
                     GDYQSKLTLMSESLRNSGRIWVPKSRETAEKIRKKEIKPSDVPEEERDYYLERKYPSFG
                     NLAPRDISSRAAKEACDDGRGVAVTGKGVFLDFADEIKRMGREWIDGQYGNLFDMYEEI
                     TDDDPHETPMMIYPASHYTMGGLWVDYNLMSNVKGLHVLGEANFSDHGANRLGASALMQ
                     GLSDGYFVIPATLPAYLTTTKPGAVTTAAPEFKAAEDEVKERINKLLNVKGTKTVDSFH
                     RELGLIMWEDCGMARTEESLTHAIERIPQLRKEFWENVRVTGTADGINAELEKACRVAD
                     FLEFAQLLCYDALNRQESCGAHFRVEYQLPDGEAKRNDDEYAYVAAWEYTGVDSLPVLH
                     KEPLTFENVHLAIRSYK"
     CDS             2494..3261
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052:Succinate dehydrogenase-2Fe-2S iron-sulfur
                     cluster binding domain (Score: -1.5; E-value: 8.6)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg48_3"
                     /sec_met_domain="Fer2_3 (E-value: 1.1e-24, bitscore: 74.4,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 3.6e-12, bitscore: 34.5,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 2e-07, bitscore: 19.2,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKTLNLTLKVWRQENREAKGRIETYAAKDIPVDASFLEMLDIVN
                     EGLVKEGKEPIHFDHDCREGICGMCSLTINGIPHGPERQVTTCQLHMRKFKDGDTVWIE
                     PFRAKAFPILRDLMVDRSALDRIIAAGGYIDVRTGSAPNANNIAVEKVKADAAFDAAAC
                     IGCGACVASCKNASAMLFTSAKIAHLNLLPQGQPERSKRVLAMMKQMEAEGFGNCTNQY
                     ECEAACPKGISVDNIARLNRDFMRANAKEGLCF"
ORIGIN
        1 tcatccacgt ctggctgacc atccagctga agctggaaaa ccatgcggcc cgcgaaccct
       61 acgtgttcaa gaacaccatc aaggcgacgc tctcctcccg ctacatgatc tataccggcc
      121 tgaccgtcct ggtgttcctg atctaccacc tgtaccagta caccctgcgc gtgggatacg
      181 accccgccca gttcaccacc ttcatttccg acggtacggt ggaaaccttt gacgtgtaca
      241 agatgatcgt gaccggattc tccaacgtat ggtgctccgc cttctacatc ctggccgttc
      301 tgatgctgtt cagccatctg cgccacgggg tacagtccat cttccagact gtgggcgtcg
      361 attcccgcaa aatccggccc ttctacaact ttatcgccat cgcctacggc gcgattatct
      421 gcctgggctt catctccgtg ccggtctcgg ttcttctggg tatcatcaaa taaccatcaa
      481 ctcctctcaa agtcatgatt aattttcccg taagcgactg gatgccggat gccaaaattc
      541 cctccggccc tattcaggac aaatggtcca agtacaaact tgagtccaaa ctgatcaatc
      601 cgaacaacaa gcgcaagtac acggtgcttg tggtaggttc cggcctggct ggcgcctccg
      661 ctgccgccac gcttgccgaa cttggctaca acgtcaaatg cttctgctac caggacagcc
      721 cccgccgcgc ccactccatt gcggcccagg gcggcattaa cgcagccaag aactaccaga
      781 acgacggcga ctccgtttac cgcctgtttt acgacaccgt taaaggcggc gacttccgcg
      841 cccgtgaagc caatgtctac cgtctggcgg aagtctcctc caacatcatt gaccagtgtg
      901 tggcccaggg cgttcccttc ggccgtgaat acggcggcct gctggacaac cgctccttcg
      961 gcggcgccca gctcaagcga accttctacg cacgcggaca gacgggccag cagctactgc
     1021 tgggtgccta ccaggccctg gaaaaggaag tggccaaggg acacattgaa atgttctccc
     1081 gccatgaaat gatggatctg gtgctggtgg acggcgaagc caagggcatc gtcacccgca
     1141 atctggttac cggcgaaatc aaatcctacg caggggatac cgtcctgctc tgcacgggcg
     1201 gctacggcaa cgtgttcttc ctgtccacca acgcgatggg ctgcaacgtc actgccgcct
     1261 acaaggccta caagcgcggt gccttcttcg ccaacccctg cttcacgcaa atccacccca
     1321 cctgcattcc ggtcaagggc gactatcagt ccaaactgac gctgatgtcg gaatccctcc
     1381 gcaacagtgg ccgcatctgg gtgcccaagt cccgtgaaac cgcagaaaaa atccgtaaaa
     1441 aggaaatcaa gccttccgat gttcccgaag aagaacggga ctactacctg gaacgcaagt
     1501 atccctcctt cggcaacctg gctccgcgcg acatttcctc ccgcgccgcc aaggaagcct
     1561 gcgacgacgg ccgcggcgtg gccgtcaccg gcaagggcgt cttcctagat tttgcggatg
     1621 aaatcaagcg catgggccgc gaatggattg acggccaata cggcaatctg tttgacatgt
     1681 acgaagaaat cacggacgac gacccccatg aaacgcccat gatgatctac cccgcctccc
     1741 actacaccat gggtggcctg tgggtggact acaacctcat gtccaatgtg aaggggctgc
     1801 acgtcctcgg tgaagccaac ttctccgacc acggcgccaa ccgcctggga gcctccgccc
     1861 tgatgcaggg cctgtctgac ggctacttcg tcattcccgc cacgctgccc gcttacctga
     1921 cgaccaccaa acccggcgcc gtcaccaccg ccgccccgga attcaaggct gcggaagacg
     1981 aagtgaagga acgcatcaac aagctcctga atgtgaaggg caccaagacg gtggactcct
     2041 tccaccggga actgggcctc attatgtggg aagactgcgg catggcccgc acggaagaaa
     2101 gcctgaccca cgccattgaa cgcattcccc agctccgcaa ggaattctgg gaaaacgtgc
     2161 gcgtaaccgg cacggcggac ggcatcaacg cggaactgga aaaagcctgc cgcgtggccg
     2221 acttcctgga attcgcccag ctgctctgct atgacgccct gaaccgccag gaatcctgcg
     2281 gcgcccactt ccgcgtggaa taccagctgc cggacggcga agctaagcgc aacgacgatg
     2341 aatacgccta tgtagccgcc tgggaataca ctggcgtgga cagcctgcct gttctccaca
     2401 aggaaccgct gaccttcgag aacgtgcacc ttgccatccg cagctacaag taaagatagc
     2461 caatacatta acactcaaaa gaaagattat aacatggcta aaacactcaa tctcactctc
     2521 aaggtctggc gccaggaaaa ccgggaagcc aagggccgca ttgagaccta tgccgccaag
     2581 gacattccgg tggacgcctc cttcctggaa atgctggaca tcgtcaatga aggcctggtc
     2641 aaggaaggca aggaacccat ccattttgac cacgactgcc gggaaggcat ctgcggcatg
     2701 tgctccctga ccatcaacgg cattccccac gggccggaac gccaggtgac cacttgccag
     2761 cttcacatgc gcaagttcaa ggatggcgat accgtctgga ttgaaccctt ccgcgcgaag
     2821 gctttcccaa tcctgcggga cctgatggtg gaccgctccg ccctggaccg catcatcgcc
     2881 gctggaggct atattgacgt gcgtaccggt tccgcgccga acgccaacaa catcgccgtg
     2941 gaaaaagtga aagcggacgc cgcctttgac gcggccgcct gcatcggctg cggcgcctgc
     3001 gtggcctcct gtaaaaacgc ctccgccatg ctgttcacct ccgccaagat cgcccatctc
     3061 aatctgctgc cgcagggcca gccggaacgt tccaagcgcg tcctggccat gatgaagcag
     3121 atggaagcgg aaggctttgg caactgcacg aaccagtatg aatgtgaagc cgcctgcccg
     3181 aaaggcatca gcgtggacaa catcgcccgc ctcaaccgcg acttcatgcg ggccaacgcc
     3241 aaggaaggcc tctgtttcta aagaagattc cttcttaaga cgctctgtaa aaactcccgg
     3301 cggcatcggc tgtcgggagt tttttcatta ccaggtttcc ttgaaccagg aataaaatac
     3361 cagacagttg cttcctcatc gtcgcgcagc gtaaaaaatt gttacaccgg aagggttcca
     3421 gtaatccagg agaccgtact tggaaatgcc ggacgaaacc taagcaagcc cccgtccgac
     3481 agcgacggcc cccatctttc ttcgagagaa agaaacattg attacaggaa ttggactgat
     3541 gcccggaagg gatggcatcc tgtcaag
//
