LOCUS       c00396_gnlPro..         3202 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|GDFPIDOG_396.
ACCESSION   c00396_gnlPro..
VERSION     c00396_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 13:50:54
            Original ID  :: gnl|Prokka|GDFPIDOG_396
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3202
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..1407
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 109.8; E-value:
                     5.8e-34)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg396_1"
                     /sec_met_domain="FAD_binding_2 (E-value: 3.7e-35, bitscore:
                     109.8, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 1.9e-39,
                     bitscore: 122.8, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="GLGKVKMYTRHEMLDVVKVDGRARGIIARNLITGKIERYAAHAVV
                     VATGGYVNTFFLSTNAMASNGSAAWQCYKKGAYFANPCMVQIHPTCVPVKGDFQSKLTL
                     MSESLRNDGRIWVPKKLEDAKALQAGTKKGKDIPEADRDYYLERRYPAFGNLVPRDVAS
                     RAAKERCDAGFGVNSTGLAVFLDFSDAINRLGKEVVKQKYGNLFDMYEEITNDNPYETP
                     MMIYPALHYSMGGLWVDYELMTSIPGLFAIGEANFSDHGANRLGASALMQGLADGYFVL
                     PYTIQNYLSDQIQVPRFSTDLPEFVEAEKAIKDRIQKLMNVKGKETVDTIHRKLGHIMW
                     EHIGMARDAKGLQEAIQMLKDLKKEFWSNVFIPGNADNLNTELEKALRVADFIEIGTLM
                     AHDALDRAESCGGHFRTEHQTEEGEALRHDDKFMYVSCWEYQGEDKDPVMLKEDLNYEF
                     VVPQTRNYKK"
     protocluster    1..3202
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:2193]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..2193
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..3202
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3202
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             1438..2193
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg396_2"
                     /sec_met_domain="Fer2_3 (E-value: 4.5e-27, bitscore: 82.5,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 8.9e-13, bitscore: 36.8,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 9.5e-07, bitscore: 17.5,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKDISVTLKVWRQRGPKEKGQFETYKIEKINQSVSFLEMLDILN
                     EQLVNEGKEPIVFDHDCREGICGMCSLYVNGHPHGPATGATTCQLYMRRFHDGETITIE
                     PWRSAGFPVIRDLMVDRYAYDKIIQAGGFTSVNTGGVPDANAIPIPKKDADLAMDAAAC
                     IGCGACAAACKNGSAMLFVSAKVSQLALLPQGKVEAARRAKAMVAKMDELGFGNCTNTR
                     ACEMECPKNISVSNIARLNREFICAKLQD"
     CDS             complement(2230..2340)
                     /locus_tag="ctg396_3"
                     /transl_table=11
                     /translation="MKKPRCKYTDFSIPVKGYNAKLCFTTYNKNKKAPFF"
     CDS             2616..3200
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Radical_SAM"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg396_4"
                     /sec_met_domain="Radical_SAM (E-value: 2.3e-17, bitscore:
                     52.0, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKKRTTSSFRAMSTERKLESIFLFVTGRCNAKCAMCFYAQEMDK
                     KQPDLTFDEIKKISETAGQFNRLWISGGEPTLREDLPEILELFYKNNHIKDVNIPTNGL
                     KPARIVEWVKRFRQNCPDCNINVSLSLDGFGTTHDTQRGVPGNFYKALETLRKIQENFG
                     DDGKVLKNIATVITKYNIDQVEDFMTWVYGRF"
ORIGIN
        1 ggtttgggta aggttaagat gtacactcgt catgagatgt tggatgtagt taaggtagac
       61 ggtcgtgctc gtggtatcat cgcccgcaac ttgattacag gtaagatcga gcgttacgcc
      121 gctcacgctg tagttgtagc tacgggtggt tacgttaata cgttcttctt gtcaaccaac
      181 gctatggcat ctaacggttc cgccgcatgg caatgctaca agaaaggcgc ttacttcgct
      241 aatccttgta tggttcagat ccacccgact tgcgtaccgg tgaaaggtga cttccaatct
      301 aaattgactt tgatgtccga atccttgcgt aacgacggcc gtatctgggt tccgaagaaa
      361 ctggaagacg ctaaagctct acaggccgga acgaagaaag gtaaagatat tcccgaggca
      421 gaccgtgatt attatttgga gcgtcgttat ccggcattcg gaaacttggt tcctcgcgac
      481 gtggcttctc gtgccgctaa agagcgttgc gacgcaggtt tcggcgtgaa cagcacgggc
      541 ttggccgtat tcttggattt ctccgacgcg atcaatcgct tgggtaaaga ggtcgttaag
      601 cagaaatacg gtaacttgtt cgatatgtac gaagagatca cgaacgataa tccatacgag
      661 actccgatga tgatctaccc ggcattgcac tactcaatgg gtggattgtg ggttgactat
      721 gagttgatga cctccattcc gggtctgttc gctatcggtg aggcaaactt ctccgatcac
      781 ggagcgaacc gtttaggtgc ttccgctttg atgcagggat tggccgatgg ttatttcgta
      841 ttaccataca cgatccagaa ctatttgtcc gaccaaatcc aagttcctcg cttcagcacg
      901 gatctgccgg agttcgtcga ggcagaaaag gccatcaagg atcgtatcca aaaattgatg
      961 aacgtcaagg gtaaagaaac cgtagacact atccaccgca aactaggtca tatcatgtgg
     1021 gaacatatcg gtatggcacg cgacgcaaaa ggtttgcaag aagctatcca gatgctgaaa
     1081 gacttgaaga aagagttctg gagcaacgta ttcatcccag gcaacgcaga caacttgaat
     1141 accgagttag agaaagcatt acgtgtagcc gactttatcg agatcggtac attgatggca
     1201 cacgatgctt tggaccgcgc tgagtcttgt ggtggtcact tccgtacgga gcaccagaca
     1261 gaagaaggtg aggccttgcg tcatgacgat aagttcatgt acgtatcttg ctgggagtac
     1321 caaggcgagg acaaagatcc tgtcatgttg aaagaggatt tgaactatga gttcgttgtt
     1381 cctcaaacac gtaactacaa gaagtaataa tcatcaatct cagaaaaaat tagaatcatg
     1441 gataaagata taagtgtaac acttaaggtt tggcgtcaga gaggtccgaa ggagaaagga
     1501 cagttcgaga catacaaaat tgagaagatc aatcagagtg tctctttctt ggaaatgttg
     1561 gatatcttga acgagcagtt ggtcaacgag ggtaaggaac cgatcgtatt cgatcacgac
     1621 tgccgcgagg gtatctgtgg catgtgttca ttgtatgtaa acggacaccc gcacggaccg
     1681 gcaactggag ctacgacttg ccagttatat atgcgtcgtt tccacgatgg agagacgatc
     1741 acgatcgaac cgtggcgttc cgcaggtttc cctgttatcc gcgacttgat ggtagaccgc
     1801 tacgcttacg acaaaatcat ccaagcgggt ggtttcacat ctgtcaacac aggtggtgta
     1861 cccgacgcta atgcgatacc tatcccaaag aaggacgcag acttagccat ggacgctgcc
     1921 gcttgtatcg gttgtggcgc ttgtgctgcc gcttgtaaga atggttccgc tatgttattc
     1981 gtatccgcta aggttagcca gctggctttg ttgcctcaag gtaaagtcga ggctgctcgc
     2041 cgtgccaaag ctatggtcgc taaaatggat gagcttggct tcggtaactg tacgaacaca
     2101 cgtgcttgcg agatggaatg tccgaagaat atctccgtta gcaacatcgc tcgtttgaac
     2161 cgcgagttca tttgcgctaa attacaagac taatatttga ttagtatatc atggaaaagg
     2221 cgtctttctc taaaagaaag gcgccttttt gtttttgtta tatgtcgtaa aacataattt
     2281 agcattatag cccttgacag gaattgaaaa gtccgtatat ttgcatcgtg gttttttcat
     2341 aatagtatta gatttaaggt taacaaagtt tggttagggc ttgtcgtgag acaggcttta
     2401 attgtttata gacctacctt ttaaacatgg accataaaat actgcaaata gagattgttt
     2461 agcgtcattt tatcgtcgat ctttgccttt aaagatcata tagtatatgc taataagtat
     2521 tttatttggc ttcttatgtt attttctccg ggtgttgatc aaacacaagg aaaaaatcat
     2581 gcgccagata agacaaatgg taaaacaaaa ataaaatgga caaaaaacga accaccagtt
     2641 ctttcagggc aatgagcacg gaaagaaaac tcgagtcgat cttccttttc gtaacaggcc
     2701 gatgcaacgc caaatgcgcg atgtgtttct atgcccaaga aatggataag aagcaacccg
     2761 acctcacttt cgatgaaatc aagaagatat cagaaaccgc cgggcaattc aaccgattat
     2821 ggatatccgg aggtgagcct accctgcgtg aggatctacc ggaaatattg gagttattct
     2881 acaaaaacaa ccatatcaaa gatgtgaaca tccctacgaa cggcttaaaa ccagcccgga
     2941 tcgtcgaatg ggtaaaacgg ttccgccaga actgcccgga ttgtaatatt aacgtaagcc
     3001 tttccttgga tggtttcggt acgacccacg atacacagag aggcgtaccg ggaaacttct
     3061 ataaggcctt ggaaaccctg cgtaagatac aggagaattt cggagacgac ggcaaggtcc
     3121 tgaaaaatat agcaaccgtt atcacgaaat acaatataga ccaggtagaa gacttcatga
     3181 cttgggtata cggacgtttc cg
//
