LOCUS       c00124_gnlPro..         6823 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|ILMKIDCG_124.
ACCESSION   c00124_gnlPro..
VERSION     c00124_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 07:52:46
            Original ID  :: gnl|Prokka|ILMKIDCG_124
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6823
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6823
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[395:3372]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      396..3372
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..6823
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6823
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             396..2636
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg124_1"
                     /sec_met_domain="Gly_radical (E-value: 1.3e-45, bitscore:
                     142.8, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 4.2e-285, bitscore:
                     935.1, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENENWAQFKGEIWKREINVRDFIQNNYKPYEGDDSFLKDSSD
                     KTKKVWDKLTEMFKVEREKGVYDAETKYPQGIDTYGPGYIDKENEVIVGLQTDAPLKRG
                     IYPKCGTRMVENSLEAYGYKLDPMTKEIFTKYRKTHNEGVFSAYTDEMLAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTEILIAEKKHYLKRLDIQELTEEIIQSREEISEQIKAL
                     KAFEKMCAGYGFDVTKPAKDAREAVQFVYLGYLAAVKDQDGAAMSLGRTSTFLDIYIEK
                     DIREGKLTEEEAQELIDQFIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTKTSYRYLHTLYNLGPAPEPNLTVLWFNNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQYFGARANLAKCLLYAINGGRDERSGVQVAPRFEPITS
                     EYLDYNEVMEKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMALHDGSVERIRATGI
                     AGLSIVADSLAAIRDTKVKVIRDERGLAVGFEREGDYVPFGNNDDRTDSIAVDITEKFM
                     EYLRQHQTYRNATPTQSILTITSNVVYGKKTGATPDGREGGTPFAPGANPMNGRDTKGA
                     VAALASVAKLPFQHAHDGISYTFAVSPATLGKERDIQIANMVGLLDGYFTPDGGQHLNV
                     NVFDKELLLDAMEHPENYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             2644..3372
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.3;
                     E-value: 1.5e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg124_2"
                     /sec_met_domain="Radical_SAM (E-value: 4.6e-37, bitscore:
                     116.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LEIKGNIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWDS
                     KGLAKYQMTPEELLAEVLRYKNFIAKGGVTVTGGEPLLQAEFLREFFRLCREAGIHTAL
                     DTSGYICTPKALAVLEYADLVLLDIKTIDPDLHPRLTAVKLDNTLRFLDELEKRGVPVW
                     IRHVIVPGLTDNDEALDKLAEYISHYTVVQKAELLPYHTMGTYKYEAQGLDYKLKEVEP
                     LSAERLANAKEIFKKHGITI"
     CDS             3461..4363
                     /locus_tag="ctg124_3"
                     /transl_table=11
                     /translation="MEQHHHDHHHHEITSLNKAFIIGISLNLAFVVAEFTAGFWYNSLG
                     LLSDAGHNLGDVASLVLAMLAFRLAKVHPTCSYTYGYKKSTVLVSLLNAVILLVAVGII
                     IAESIGKLLHPRPVEGDAIAWVAGIGVVINALTAWLFMKDKDKDLNVKGAYLHMAADAL
                     VSVGVVVSGIVIAHTGWYIIDPIIGLLIAFVIIVSTWGLLRDSIRLSLDGVPAGTDIDK
                     VEKTIHSVEGVEGVHHLHIWAISTTETALTAHVSVADISEMERIKQEIKHDLQHVGIQH
                     ATLEFETSGFKCDGRCCSD"
     CDS             4524..5090
                     /locus_tag="ctg124_4"
                     /transl_table=11
                     /translation="MEPIINSQAPEFKVQAYHNGSFKTVSSDDIKGKWAIFFFYPADFT
                     FVCPTELVDMADKYDQFQAMGVEVYSVSTDSHFVHKAWHDASETIRKIKYPMLADPTGV
                     LSRAFGVMIEEDGMAYRGTFLVNPEGKIKLAEINDNGIGRNADELLRKVEAAQFIAEHP
                     SEVCPAKWKKGGETLKPSIDLVGKI"
     CDS             5184..6590
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg124_5"
                     /sec_met_domain="Pyr_redox_2 (E-value: 5.2e-35, bitscore:
                     109.4, seeds: 65, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLDSSMRNQLSGIFSGLTNEYIFDIQVSPEHESRKELLELLNDVA
                     SCSDKITVQVADGEGLEFSLLKNGEKTGIKFRGVPNGHEFTSLLLAILNSDGKGKNIPD
                     ESICNRVKALNGPVKLTTYVSLTCTNCPDVVQALNAMATLNPEISHEMVDGAINQEEVA
                     AMKVQGVPSVFADGKLIHVGRGDFGELLGKLEAQYGVKEENLPVVEKNYDVIIVGGGPA
                     GSASAIYTARKGLAVAVVADRIGGQVKETVGIENMISVSQTTGQQLALDLMSHMKDYPI
                     DILEHRRIEKVEVEGREKLLTTSTGEKLIAPAVIVATGASWRRLNVPGESEYIGRGVAF
                     CPHCDGPFYKGKHVAVVGGGNSGVEAAIDLAGICSKVTLLEFADELKADQVLQDKARSL
                     PNVEIFMHSQTMEVLGNGEKVTGIRIKDRKTEEVRNIELDGIFVQIGLAANSGPFXXXX
                     XXXXXXXXDR"
ORIGIN
        1 ataggtattg tgtactcccc ttgacggctc tatctttgca acatagaaaa aagtaacact
       61 attagtataa tcgctatatc aaaacctttt gctaattatg taagcatata agatgattaa
      121 acgtgtgtcg gaaatttttt attggttgtt tccggcataa aaaagggact ctcctgcgaa
      181 ggatggttcc ttttttattt ttttgatatt atttataatc cttcctgttt gatacctata
      241 tcaggcgata cgctgcacta tatctaccgt tttagtaact atttgtaggg attttggccg
      301 ttgggaatac atgatgtttt tgttgattgt ttcgcatgcc atataaataa cgtacctttg
      361 caccgaaatt aaaaccgaag aaaacaaaat aaggtatgaa gtttgaaaac gagaactggg
      421 cgcagttcaa aggcgaaatt tggaaaagag agattaacgt aagggatttt atccagaaca
      481 actacaaacc ttacgaaggg gacgactctt tcttgaaaga ctcctcagat aagacgaaga
      541 aagtttggga taaattgacc gaaatgttta aggttgaaag ggagaagggt gtttacgacg
      601 ctgaaacaaa atatcctcaa ggtatagata cttacggacc aggttatatt gacaaagaaa
      661 acgaagtaat tgtcggcctg caaaccgatg cccccctgaa acgcggtatt tacccgaaat
      721 gtggtacccg catggtggaa aacagtctgg aagcatatgg ctacaaactg gacccgatga
      781 caaaagaaat cttcacgaaa tacagaaaga cgcataacga aggcgtattc tccgcttata
      841 cagatgaaat gcttgcagcg cgccgttccg ctatcatcac cggcctgccg gatgcttacg
      901 gacgcggacg tattatcggt gactaccgcc gtgtagcact ttacggtact gaaatcctga
      961 tcgctgaaaa gaaacattat ctgaaaagac tcgatattca ggaactgacc gaagagatca
     1021 tccagagccg tgaggaaata tccgaacaga tcaaggcgct gaaggctttc gaaaagatgt
     1081 gtgccggtta tggtttcgac gtgaccaagc ctgcaaagga tgcacgcgaa gcggttcagt
     1141 ttgtctatct gggttatctg gctgcggtga aggaccagga tggggctgcc atgtcgttgg
     1201 gacgtacttc tactttcctc gatatttata ttgaaaaaga tatccgcgaa ggcaagctga
     1261 cggaagaaga agctcaggag ctgatcgatc agtttattat caaactgcgt atcgtccgtt
     1321 tccttcgtac tccggaatat aacgacctgt tctccggtga ccctgtttgg gtaaccgagt
     1381 ctttgggcgg acagggtgtc gacggccgtt cattggtgac gaagacgtct taccgttatc
     1441 tgcatacatt atataacctc ggcccggcac cggaacccaa cttaacggta ctgtggttca
     1501 acaacgctcc tgaaaactgg aaacgtttct gtgcgaaggt ttctatcgat acttccgcta
     1561 tccagtatga gaacgacgat ctgatgcgtc cggattatgg agatgattat ggtattgcct
     1621 gctgcgtgtc tccgatgaag atcggtaagc agatgcagta tttcggcgca cgtgccaacc
     1681 tggctaaatg tttgctgtat gccatcaacg gcgggcgcga cgaaagatca ggtgtacagg
     1741 tggctccgag gtttgaaccg atcaccagcg aatacctgga ttacaacgaa gtgatggaga
     1801 aatacgaaca gatgatgcgc tggctggcta aagtttatgt gaatgcactg aagatcatcc
     1861 attacatgca cgataaatat gcgtatgaag cattcgagat ggctttgcat gacggttcgg
     1921 tagaacgtat ccgcgcaaca ggtattgccg gcctttctat cgttgccgac tcattggctg
     1981 ctatccgcga tacgaaagta aaagtgatcc gcgacgaacg tggcctggct gtcggtttcg
     2041 aacgtgaagg agattatgtt cctttcggta acaatgacga tcgtacagat agtattgctg
     2101 tcgacatcac tgaaaagttc atggaatatc tgcgtcagca ccagacatac cgcaacgcca
     2161 ctccgacaca gtctatcctg actatcacat ccaatgtggt gtatggaaag aagacaggtg
     2221 caactcccga cggtcgcgaa ggtggtacgc cgtttgctcc gggtgcgaac ccgatgaacg
     2281 gacgtgatac caaaggcgct gttgctgcac tggcttctgt tgctaaactg ccgttccagc
     2341 atgcacacga cggtatctct tatacattcg ctgtttctcc tgcaacgtta ggaaaagaac
     2401 gtgatattca gatcgccaat atggtcggcc tgctggacgg ttactttact cctgacggcg
     2461 gacagcatct gaatgtgaac gtattcgaca aggagctgtt gctcgatgct atggagcatc
     2521 cggaaaacta tccgcagttg acaatccgcg tatcgggtta tgcagtaaac tttgtgaaac
     2581 tgacacgcga acagcagctg gatgtgattt caagaacaat caatcattct ttgtaattga
     2641 aagttggaaa ttaaaggaaa catacattca ctagagagtt tcgggaccgt tgatggtccc
     2701 ggaattcgtt ttgttgtctt tatgcagggt tgtccgctcc gttgcctgta ttgccacaat
     2761 ccggatacct gggatagcaa aggtcttgcc aaatatcaga tgacgcctga agaattattg
     2821 gctgaggttt tacgatataa gaactttatc gccaagggag gcgtgactgt caccggggga
     2881 gaacctttat tgcaagctga atttctcagg gagtttttcc gtctttgccg ggaagccggt
     2941 attcatacgg cgctcgatac atccggctat atctgcacac cgaaagccct ggcggttctg
     3001 gaatatgccg acctggtctt attggatata aaaaccatcg atcccgacct gcatccccgt
     3061 ctaacggccg tgaaactgga taatacgtta cgtttcctgg acgaactgga aaaacgcggt
     3121 gttccggttt ggatacgcca tgtcattgtg cccggactga cggataatga cgaagccttg
     3181 gataaactgg ccgaatacat cagccactat acggttgtac agaaggcgga actcttgcct
     3241 tatcacacga tgggcacata taaatacgag gctcaaggtc tcgactacaa actgaaagag
     3301 gtagaacctt tatcggccga acgtctggca aatgccaaag aaatatttaa gaagcacggt
     3361 ataaccatct gataatttac ctataatcag gtatattttt attgctctaa aaaacatagt
     3421 tttgtcccat attgttactc aaataaaagt aggaatgaat atggaacaac atcatcatga
     3481 tcatcaccac cacgaaataa cctctttgaa caaggctttc attataggta tctccctgaa
     3541 cctggctttt gtcgtagccg aatttacggc tggcttctgg tataattcat taggacttct
     3601 gtcggatgca gggcataacc tgggagatgt ggccagcctg gtactggcta tgctggcttt
     3661 ccggctggct aaggtgcacc cgacttgcag ctatacctat ggatataaga aaagcactgt
     3721 actcgtatcc ctcctgaatg cggtcatcct cttggtggcc gtcgggatta ttattgctga
     3781 aagtatagga aaattgctgc atccgcgtcc ggtcgagggt gatgcgatcg cttgggtggc
     3841 aggtatcgga gtcgttataa atgcacttac ggcatggctt ttcatgaagg ataaggataa
     3901 agacttgaat gtgaaaggtg cttacttgca tatggctgcc gatgcactgg tatcggtcgg
     3961 cgtggttgtc tccggaattg ttattgccca taccggatgg tatatcatcg acccgattat
     4021 cggcctgttg attgcgttcg ttatcattgt ttctacctgg ggactgctac gcgacagtat
     4081 ccgtctctct ctggacggtg tgccggcggg aacggacatc gataaggtgg aaaagacgat
     4141 acattcggtc gaaggcgttg aaggcgtcca tcatctccat atctgggcga tcagtacgac
     4201 cgaaacggca cttaccgccc atgtttccgt tgcggatatt tcagaaatgg aacgtatcaa
     4261 acaggagata aaacacgacc ttcagcatgt gggaatccag catgctacgc tcgaatttga
     4321 aacgtcggga ttcaagtgcg atgggcgctg ttgctcagac tgaaacaggt atattataaa
     4381 cctgatttac tgatactcaa agattttgtc tatgactgca taaaaccttt cgattggatt
     4441 ttttgttctt acctcgaatc ggcaacttat ctttgcacca gtaacaaaaa caaaatagta
     4501 ataacgatta aaataaaaat tctatggaac ctattatcaa ttcacaagca cccgagttta
     4561 aagttcaggc ataccataac ggtagtttca aaactgtaag cagcgacgac atcaaaggca
     4621 aatgggcgat tttctttttc tacccggctg actttacatt tgtttgtccg accgagttgg
     4681 tagatatggc tgacaaatat gaccagttcc aggcaatggg tgtagaagtt tactctgtaa
     4741 gtacagattc acacttcgta cacaaagcat ggcacgatgc ttctgaaacg attcgtaaga
     4801 tcaaataccc gatgttggca gacccgacag gcgtattgag ccgtgcattc ggtgtaatga
     4861 tcgaagaaga cggtatggct taccgcggta cattcctggt aaatccggaa ggaaagatca
     4921 aactggcaga aatcaacgat aacggtatcg gccgtaacgc cgacgaactg cttcgtaaag
     4981 tagaagctgc ccagttcatc gccgaacatc cgagcgaagt ttgccccgcc aaatggaaaa
     5041 aaggtggcga aacattgaaa ccgagcatcg atctggtcgg taagatctaa tagtaaaact
     5101 ggaggccggc aacatattgt cggccttttt tatctaaaca aatatcagtt gttctattca
     5161 taattaaaat aaaggattca actatgttag attcatcaat gagaaatcag ttgtccggta
     5221 tctttagcgg attgacgaac gaatatatat ttgatataca agtttctcct gaacatgaaa
     5281 gcaggaagga actattggaa ttgctgaacg atgtggcttc ctgttccgat aagatcaccg
     5341 tacaggtggc agacggcgaa ggcctggagt tctctctgct gaaaaacggg gaaaaaaccg
     5401 gtattaaatt ccggggcgtt cccaacggac acgaatttac ttcgttgctg ctggctatcc
     5461 tgaacagcga cggcaaaggc aaaaatattc ccgacgaaag tatttgtaac cgtgtgaagg
     5521 ccctgaatgg cccagtcaaa ctgactacat atgtttcttt gacctgtacc aactgtcctg
     5581 acgttgtaca ggctttgaat gccatggcta cgctgaaccc ggaaatctct cacgaaatgg
     5641 tggacggtgc gatcaaccag gaagaggttg ctgccatgaa agtgcaggga gtaccttccg
     5701 tatttgccga cggtaaactg atccatgtag gtcgtggaga ctttggcgaa ctgcttggca
     5761 agctcgaagc gcagtatggc gtgaaagagg agaacctgcc ggtggtagaa aagaactacg
     5821 atgttatcat cgtcggcgga ggtcctgccg gaagtgcgtc agctatctat actgcccgta
     5881 aaggtttggc ggtggctgtt gtggccgacc gtatcggcgg acaggtgaag gaaactgtcg
     5941 gtatcgagaa tatgatttct gtttcgcaga cgacggggca gcagctggct ctcgacctga
     6001 tgtcgcatat gaaagattat ccgatcgata tccttgaaca ccgccgtatc gagaaggtgg
     6061 aagtcgaagg acgcgaaaag ctgctgacaa cctctaccgg cgaaaaactg atagccccgg
     6121 ccgttatcgt agcaaccgga gctagctggc gcaggttgaa tgtccccgga gaatccgaat
     6181 atatcggacg cggtgtcgct ttctgcccgc attgcgacgg tccgttctat aaaggcaagc
     6241 atgtggctgt tgtaggcggc ggaaattcag gcgttgaggc ggctatcgac ctggcaggta
     6301 tctgttcaaa agtgacgctt ctcgaatttg ccgatgagtt gaaagcggat caggtcttgc
     6361 aggataaggc aagaagcctt ccgaacgtgg agatctttat gcattcccag acgatggagg
     6421 tactgggtaa cggtgaaaaa gtgaccggta tccgtataaa ggaccgtaag acagaggaag
     6481 tccgcaacat cgaactggat ggcatctttg tccagatcgg cctggcagcc aacagcggtc
     6541 cgttccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgatcgatag ccattgtcgt
     6601 acaagcgttc caggtatcta tgcggccggc gacgtatcga ctgtccctta taaacagatc
     6661 attatttcga tgggcgaagg ggccaaagca gccctgacag ctttcgaaga ccggatgcgg
     6721 ggaatgttat aagccgcaga actttccgga aggcataaaa aataataccc gggtataaat
     6781 cgattcagaa gcgtttctat gtcggtttat atccgggtat tgc
//
