LOCUS       c00169_gnlPro..         7654 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|HJNMANDC_169.
ACCESSION   c00169_gnlPro..
VERSION     c00169_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 13:25:07
            Original ID  :: gnl|Prokka|HJNMANDC_169
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7654
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..1560
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 108.8; E-value:
                     1.1e-33)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg169_1"
                     /sec_met_domain="FAD_binding_2 (E-value: 5.5e-35, bitscore:
                     108.8, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 3.8e-39,
                     bitscore: 121.4, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="QCVAQGVPFGREYGGLLDNRSFGGAQLKRTFYARGQTGQQLLLGA
                     YQALEKEVAKGHIEMFSRHEMMDLVLVDGEAKGIVTRNLVTGEIKSYAGDTVLLCTGGY
                     GNVFFLSTNAMGCNVTAAYKAYKRGAFFANPCFTQIHPTCIPVKGDYQSKLTLMSESLR
                     NSGRIWVPKSRETAEKIRKKEIKPSDVPEEERDYYLERKYPSFGNLAPRDISSRAAKEA
                     CDDGRGVAVTGKGVFLDFADEIKRMGREWIDGQYGNLFDMYEEITDDDPHETPMMIYPA
                     SHYTMGGLWVDYNLMSNVKGLHVLGEANFSDHGANRLGASALMQGLSDGYFVIPATLPA
                     YLTTTKPGAVTTAAPEFKAAEDEVKERINKLLNVKGTKTVDSFHRELGLIMWEDCGMAR
                     TEESLTHAIERIPQLRKEFWENVRVTGTADGINAELEKACRVADFLEFAQLLCYDALNR
                     QESCGAHFRVEYQLPDGEAKRNDDEYAYVAAWEYTGVDSLPVLHKEPLTFENVHLAIRS
                     YK"
     protocluster    1..7654
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:2368]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..2368
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..7654
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7654
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             1601..2368
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052:Succinate dehydrogenase-2Fe-2S iron-sulfur
                     cluster binding domain (Score: -1.5; E-value: 8.6)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg169_2"
                     /sec_met_domain="Fer2_3 (E-value: 1.1e-24, bitscore: 74.4,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 3.6e-12, bitscore: 34.5,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 2e-07, bitscore: 19.2,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKTLNLTLKVWRQENREAKGRIETYAAKDIPVDASFLEMLDIVN
                     EGLVKEGKEPIHFDHDCREGICGMCSLTINGIPHGPERQVTTCQLHMRKFKDGDTVWIE
                     PFRAKAFPILRDLMVDRSALDRIIAAGGYIDVRTGSAPNANNIAVEKVKADAAFDAAAC
                     IGCGACVASCKNASAMLFTSAKIAHLNLLPQGQPERSKRVLAMMKQMEAEGFGNCTNQY
                     ECEAACPKGISVDNIARLNRDFMRANAKEGLCF"
     CDS             2917..7653
                     /locus_tag="ctg169_3"
                     /transl_table=11
                     /translation="MNPPHRSSSAHRSAVPAYRKWYFGAGGSPGRRGMEAAPERDALPF
                     EAIRIHEERNVGPPSTSADGYSAHGTFTIPEGAEGKKLYGTCSLFLGVDDWGILEVKDS
                     GGNVVAQVDLKENPQTAGEQGGHKYHTGTGGAQLPSGTYSWEVSQTNIDYNPASGNTSI
                     CNYSIDVVPTEPGGRKEPEPCPCEGDTCDNSGGTPPSPSQARSGPEAGMKSGALGNYSS
                     AGCSVTAESTATLMYWSCNFGAFRGLGGLPAGRVELRGEENVSGLERPSSLAYNHPLNS
                     RLDVPEGGIVPGARFNLVQGDRVIAMRCYTDGSVLPIGVDTSGGGRAALATVEGQSCLR
                     WVVEDGSQYLFSAETGTLLSYTTTDRQVISNASSYLDVRHAGDGSLRQIWNLWDGLLNV
                     ENVTSTGYVIALYTPGQITGTDGQGFYTVTGAPLKTFTLSLDAEEKFTITEQAPARQPY
                     AVTWWNDGLAWNMRQGTGEDALTTLRTRTELEPENSVWQLVTEISKNGIVAARTCAIYQ
                     TTDVGDLLLTLAEGYGSPEEQTTQYAYDQCGRLRTETAPDGSQIHYTYDLYGRLLSRDE
                     PWAESGRRITRYTYACSGEADFSNEPATETADLLPLEGHVKTLTSTTWKYTTANHIKRT
                     ERRVTGLGVAGTRLTAEEQWLAGAANIHARGRTRFSRDLDGVQTWHDYAATTEHGALYT
                     ETVETRINGEAVPGQSTRAVTWITAEGQRVREENYLLLSTGQWALTGSAVYEFDTQNRW
                     VKRTAGNGRLTERELMCDGGLLWEIDENGIRTDYAYDTARQLVEVTRSAVMDGETVITP
                     ETITTYARDAAGRVLSTRQDTGAMTTQESTEYDLLGRMTSSTDVLGRVTTYAYSQDGLT
                     VTQTVPSGATFITRSAPDGTVMEESGTGQRHVIYAIDLVSDGVRTFTKAVSGETQTELQ
                     RSIVNGAGETLRTGVPNTVGGVIYTRNTYNARGQLTKTQTDAGNAATTMAPTLWEYDAF
                     GNKTKETWKLADPATVSNSRITTWSYGVEQARDEVYRVVTATRNNSRGTTYNETQKTLA
                     SSLSSTLESKVISIDPRGNASEQWSEYGPGAVRTQKSSIPTSDITAAATVIDGFIISQT
                     DHAGVTATHTRAYTETGVIYASTDGRGNTTTTKTDIAGRTVSVTDAAGNTTSTAYGPWF
                     DQPAVVTNALGNTTCYGYDLRGRNTAQWGTGAQPLLFGYDEADRMISLTTFREDAGDIT
                     ADPTGRTDGDVTTWSYDDATGLLIRKTWADGTHEDTAYNALNFKSTLTDARGVVTTWGY
                     NLKKGVNNSVSYSDSTPGIQYAYNHLNQLTQVTDASGSRVLTYTPCNEPDTDSITIGGS
                     SYQLQEHYDTYGRSSGYTLKQGTDVLQEASQGYEADGRLASAGIRHGGTEQSFAYGYLA
                     GSSLLSSLAMPDGIVRELAYEQRRNLVTAINCRLGETVLVSRSQGYDALGRPVTRTQQR
                     GTEPARSDSFSYNGRNELTAATLGAAPYGYSYDNIGNRKTAREPAEELAYAANELNQYT
                     GIEESGETPFVPTYDASGNQTLIKTSTGVWTVVYNAANRAVSFTSRNGNTIIECGYDYQ
                     "
ORIGIN
        1 cagtgtgtgg cccagggcgt tcccttcggc cgtgaatacg gcggcctgct ggacaaccgc
       61 tccttcggtg gcgcccagct caagcgaacc ttctacgcac gcggacagac gggccagcag
      121 ctcctgctgg gtgcctacca ggccctggaa aaggaagtgg ccaagggaca cattgaaatg
      181 ttctcccgcc atgaaatgat ggatctggtg ctggtggacg gcgaagccaa gggcatcgtc
      241 acccgcaatc tggttaccgg cgaaatcaaa tcctacgcag gggataccgt cctgctctgc
      301 acgggcggct acggcaacgt gttcttcctg tccaccaacg cgatgggctg caacgtcact
      361 gccgcctaca aggcctacaa gcgcggtgct ttcttcgcca acccctgctt cacgcaaatc
      421 caccccacct gcattccggt caagggcgac taccagtcca aactgacgct gatgtcggaa
      481 tccctccgca acagcggccg catctgggtg cccaagtccc gtgaaaccgc agaaaaaatc
      541 cgtaaaaagg aaatcaagcc ttccgatgtt cccgaagaag aacgggacta ctacctggaa
      601 cgcaagtatc cctccttcgg caacctggcc ccgcgcgaca tttcctcccg cgccgccaag
      661 gaagcctgcg acgacggccg cggcgtggcc gtcaccggca agggcgtctt cctggatttt
      721 gcggatgaaa tcaagcgcat gggtcgcgaa tggattgacg gccaatacgg caatctgttt
      781 gacatgtacg aagaaatcac ggacgacgac ccccatgaaa cgcccatgat gatctacccc
      841 gcctcccact acaccatggg cggcctgtgg gtggactaca acctcatgtc caatgtgaag
      901 gggctgcacg tcctcggtga agccaacttc tccgaccacg gcgccaaccg cctgggagcc
      961 tccgccctga tgcagggcct gtctgacggc tacttcgtca ttcccgccac gctgcccgct
     1021 tacctgacga ccaccaaacc cggcgccgtc accaccgccg ccccggaatt caaggctgcg
     1081 gaagacgaag tgaaggaacg catcaacaag ctcctgaatg tgaagggcac caagacggtg
     1141 gactccttcc accgggaact gggcctcatt atgtgggaag actgcggcat ggcccgcacg
     1201 gaagaaagcc tgacccacgc cattgaacgc attccccagc tccgcaagga attctgggaa
     1261 aacgtgcgcg taaccggcac ggcggacggc atcaacgcgg aactggaaaa agcctgccgc
     1321 gtggccgact tcctggaatt cgcccagctg ctctgctatg acgccctgaa ccgccaggaa
     1381 tcctgcggcg cccacttccg cgtggaatac cagctgccgg acggcgaagc taagcgcaac
     1441 gacgatgaat acgcctatgt agccgcctgg gaatacactg gcgtggacag cctgcctgtt
     1501 ctccacaagg aaccgctgac cttcgagaac gtgcaccttg ccatccgcag ctacaagtaa
     1561 agatagccaa tacattaaca ctcaaaagaa agattataac atggctaaaa cactcaatct
     1621 cactctcaag gtctggcgcc aggaaaaccg ggaagccaag ggccgcattg agacctatgc
     1681 cgccaaggac attccggtgg acgcctcctt cctggaaatg ctggacatcg tcaatgaagg
     1741 cctggtcaag gaaggcaagg aacccatcca ttttgaccac gactgccggg aaggcatctg
     1801 tggcatgtgc tccctgacca tcaacggcat tccccacggg ccggaacgcc aggtgaccac
     1861 ttgccagctt cacatgcgca agttcaagga cggcgatacc gtctggattg aacccttccg
     1921 cgcgaaggct ttcccaatcc tgcgggacct gatggtggac cgctccgccc tggaccgcat
     1981 catcgccgct ggaggctata ttgacgtgcg taccggttcc gcgccgaacg ccaacaacat
     2041 cgccgtggaa aaagtgaagg cggacgccgc ctttgacgcg gccgcctgca tcggctgcgg
     2101 cgcctgcgtg gcctcctgta aaaacgcctc cgccatgctg ttcacctccg ccaagatcgc
     2161 ccatctcaat ctgctgccgc agggccagcc ggaacgttcc aagcgcgtcc tggccatgat
     2221 gaagcagatg gaagcggaag gctttggcaa ctgcacgaac cagtatgaat gtgaagccgc
     2281 ctgcccgaaa ggcatcagcg tggacaacat cgcccgcctc aaccgcgact tcatgcgggc
     2341 caacgccaag gaaggcctct gtttctaaag aagattcctt cttaagacgc tctgtaaaaa
     2401 ctcccggcgg catcggctgt cgggagtttt ttcattacca ggtttccttg aaccaggaat
     2461 aaaataccag acagttgctt cctcatcgtc gcgcagcgta aaaaattgtt acaccggaag
     2521 gcacccagta atccaggaga ccgtacttgg caatgccgga cgaaacctaa gcaggccccg
     2581 tccgacagcg acggccccat ctttctccga gaaagaaaaa cattgattac aggaattaga
     2641 ctgatgcccg gaagggatgg cctcctgtca agggctcagc atgagaggaa aacattttct
     2701 aacaaacaga caacattgat gtctattaag ggaatgcact gagcgtaaga attataatat
     2761 tattttctca tggaataaat tctcatgttc ttaaaataac agaaattttt caaataaatt
     2821 cttattgacg ctaaatctga ataatgctac taggaaaata ataatagagg gttgttctct
     2881 tctaatttca ttatcaatac taactcttaa taccttatga atccccctca tcgttcttcc
     2941 tccgcccatc gttctgccgt tcccgcttac cggaaatggt actttggagc cggcggcagc
     3001 cccggcaggc ggggcatgga ggcagctccg gagcgcgacg cgctgccgtt tgaagcgatc
     3061 cggatccatg aggaaaggaa cgtggggccg ccgagcacgt cggcggacgg gtacagcgcg
     3121 cacgggacat tcaccatccc ggaaggagcg gagggcaaaa aactctacgg cacctgctcg
     3181 ctcttccttg gggtggacga ctggggaatc ctggaagtaa aggactccgg cggcaacgtg
     3241 gtggcgcagg tggatctgaa ggaaaacccg cagacggcgg gcgagcaggg cggccacaaa
     3301 taccacacgg ggaccggcgg ggcgcagctg ccctccggca cctacagctg ggaagtcagc
     3361 cagaccaaca tcgactacaa tccggcaagc ggcaacacct ccatctgcaa ctacagcatc
     3421 gacgtggtgc cgacggaacc ggggggcagg aaagaacccg aaccgtgtcc ctgcgaggga
     3481 gacacgtgcg acaacagcgg cggaacgccg ccttctccgt cgcaggcccg gtcgggccct
     3541 gaggcgggca tgaaaagcgg cgccctggga aactacagct cggcggggtg cagcgtgacg
     3601 gcggaaagca cggccacgct gatgtactgg tcctgcaact tcggagcgtt ccgtggactt
     3661 gggggccttc cggccggaag ggtggaactg aggggtgaag aaaacgtctc cggcctggaa
     3721 agaccctcct cactggccta caaccatccc ctgaacagcc gtctggacgt gccggaaggg
     3781 ggcattgtcc ccggagcacg gtttaatctg gtgcagggag accgggtgat tgccatgcgc
     3841 tgctacacgg acgggtccgt gctgcccatc ggggtggaca cgtcgggcgg agggcgtgcg
     3901 gcgctggcca cggtggaagg acaatcctgc ctgcgctggg tggtggaaga cggcagccaa
     3961 tacctcttct cggcggaaac gggaacgctc ctctcctaca ccaccacgga caggcaggtc
     4021 atctccaacg cgtcatccta tctggacgtc aggcatgccg gagacggctc gctgaggcaa
     4081 atctggaacc tgtgggacgg cctgctcaac gtggaaaacg tcacctccac gggttacgtc
     4141 atcgcgctct atacccctgg gcaaatcacc ggaacggacg gacagggatt ttataccgtt
     4201 acgggcgctc ccttgaaaac atttactctt tccctggatg ctgaggaaaa gttcaccatc
     4261 acggaacagg cgcctgccag gcagccctat gccgtcacct ggtggaacga cggcctggcg
     4321 tggaacatgc ggcagggcac gggggaagac gccctcacga ccctccgcac gcgcacggag
     4381 ctggaaccgg aaaactcggt ctggcagctg gtcacggaaa tctccaaaaa cggaatcgtg
     4441 gcggcgcgca cctgcgccat ctaccagacc acggacgtgg gcgacctgct gctcacgctg
     4501 gcggaaggct acggaagccc ggaggagcaa accacgcaat acgcctacga ccagtgcggg
     4561 cggctcagga cggaaacggc cccggacggc agccagatcc actacacgta tgacctctac
     4621 ggccgcctgc tcagccggga cgaaccgtgg gcggaaagcg gcaggcgcat cacgcgctac
     4681 acctacgcct gttcgggaga agccgacttc agcaacgaac ccgccacgga aacggcagac
     4741 ctgcttccgc tggaaggaca cgtcaaaacg ctgacatcca ccacctggaa atacacgacg
     4801 gccaaccaca tcaaaagaac ggaacggcgg gtcacgggcc tgggtgtggc gggcacgcgc
     4861 ctgacggcgg aggaacaatg gctggccgga gccgccaaca tccatgcccg cggacgcaca
     4921 cggttcagcc gggacctcga cggcgtgcaa acgtggcacg actacgcggc cacgacggag
     4981 cacggcgccc tctacacgga aacggtggaa acgcgcatca acggagaagc cgtgccggga
     5041 caaagcacgc gcgccgtcac ctggatcacg gcggaagggc agcgcgtcag ggaagaaaac
     5101 tacctcctgc tttccaccgg gcaatgggcg ctcacgggca gcgccgtcta cgaatttgac
     5161 acgcagaacc ggtgggtgaa gcggacggcg ggcaacggcc ggctcacgga acgcgaactg
     5221 atgtgcgacg gaggcctgct gtgggaaatc gatgaaaacg gcatcaggac ggactacgcc
     5281 tacgacacgg cgcgccaact ggtggaagtc acgcgttccg ccgtgatgga cggggaaacc
     5341 gtcatcacgc cggaaaccat caccacctac gcccgggatg cggcagggcg cgtgctctcc
     5401 acgcgtcaag acacgggggc gatgactacc caagaaagca cggagtacga cttgctgggg
     5461 agaatgactt cctctacgga cgtcctgggc cgggtcacta cctacgccta cagccaggac
     5521 ggcttgacgg tcacgcaaac cgtcccttcc ggggctacat tcatcacgcg cagcgcgccg
     5581 gacggaacgg tgatggaaga atccggcacg gggcagcggc acgtcatcta cgccatcgac
     5641 ctggtcagcg acggtgtgcg gaccttcacg aaagccgtct ccggggaaac gcaaaccgag
     5701 ctgcagcgca gcattgtcaa cggagccggg gaaaccctgc gcacgggcgt ccccaacacc
     5761 gtcggcggag tcatttacac gaggaacacc tacaacgcca gggggcagct caccaaaacg
     5821 cagacggacg cgggcaatgc ggccacgacg atggccccga ccctgtggga atacgacgcc
     5881 ttcggcaaca aaacgaaaga aacctggaaa ctcgccgatc cggctacggt gtccaactcg
     5941 cgcatcacca cgtggagcta cggcgtggaa caggcccggg atgaagtata ccgcgttgtt
     6001 acggcgacca ggaacaacag ccggggaacg acctataacg aaacgcagaa aacgctggct
     6061 tcctccctct cgtccacgct ggaaagcaaa gtcatttcca tcgatcccag gggaaacgct
     6121 tccgaacaat ggagcgaata cggtccgggc gccgtccgga cgcagaaaag cagcatcccc
     6181 acctccgaca tcacggccgc cgctacggtc atcgacggtt ttatcatctc gcaaacggac
     6241 catgcgggcg tcacggccac gcatacccgc gcctacacgg aaaccggcgt catctacgcc
     6301 agcacggacg gccggggcaa cacgaccacg acgaaaaccg atatcgccgg gcgcaccgtt
     6361 tcggtgacgg acgcggcggg caacacgact tctaccgcct acggcccctg gtttgaccag
     6421 cctgccgtcg tcaccaacgc cctgggcaac acgacctgct acggctacga cctccggggc
     6481 cgcaacacgg cgcaatgggg aacgggggcc cagcccctgc tcttcggcta tgacgaggca
     6541 gacaggatga taagcctcac cacgttccgg gaggacgcgg gcgacatcac cgccgacccc
     6601 acgggacgca cggacgggga cgtcactacg tggagctacg atgacgccac gggcctgctc
     6661 atccgcaaaa cctgggcgga cggaacgcat gaagacaccg cctacaatgc cctgaacttc
     6721 aaatccacgc tcacggacgc gcggggggtg gtcaccacct ggggctacaa cctgaaaaag
     6781 ggggtcaaca actccgtctc ctacagcgac tccacgcccg gcatccagta cgcctacaac
     6841 cacctcaacc agctgaccca ggtcacggac gcctccggct cgcgcgtcct cacgtacacc
     6901 ccctgcaacg aaccggacac cgacagcatc accatcggag ggagctctta ccagctccag
     6961 gaacactacg acacttacgg acgctcctcc ggctataccc tgaaacaggg aaccgacgtc
     7021 ctccaggaag ccagccaggg ctatgaagcc gacggaaggc tggccagcgc cggaatcagg
     7081 cacgggggaa cggagcaaag cttcgcctac ggctacctgg caggaagcag cctgctctcc
     7141 agccttgcga tgcccgacgg catcgtccgg gaacttgcct atgaacagcg ccgcaacctg
     7201 gtcacggcaa tcaactgccg cctgggggaa accgtgctgg tctcccgcag ccagggctac
     7261 gatgccctgg gacgcccggt cacccgcacc cagcagcgtg gaacggaacc cgcccgcagc
     7321 gacagcttca gctacaacgg cagaaacgaa ctcaccgccg ctaccctggg cgccgccccc
     7381 tacggctaca gctacgacaa catcggcaac cgcaagacgg cacgggaacc ggccgaggaa
     7441 ctcgcctacg cggccaacga gctcaaccaa tacaccggca ttgaagaaag cggggaaacg
     7501 ccttttgtgc cgacgtacga cgcctcgggc aaccagaccc tcatcaagac gtcaacgggc
     7561 gtgtggaccg tggtctacaa cgcggccaac cgcgcggtga gcttcaccag ccggaatgga
     7621 aacacgatta ttgagtgcgg ctacgattac cagg
//
