LOCUS       c00033_gnlPro..        14578 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|IHACFGHE_33.
ACCESSION   c00033_gnlPro..
VERSION     c00033_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 09:54:31
            Original ID  :: gnl|Prokka|IHACFGHE_33
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 14578
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..246
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: Fer4"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_7"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_6"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1020:4Fe-4S binding domain (Score: 30.3; E-value:
                     8.1e-10)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg33_1"
                     /sec_met_domain="Fer4_7 (E-value: 4.4e-13, bitscore: 41.1,
                     seeds: 208, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4 (E-value: 4.9e-10, bitscore: 30.4,
                     seeds: 528, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_2 (E-value: 4.2e-08, bitscore: 24.4,
                     seeds: 214, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_6 (E-value: 1.1e-08, bitscore: 26.4,
                     seeds: 134, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 2.9e-06, bitscore: 19.0,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="ACIGCGKCVKVCPFEAITLENNLAYIDPNKCKSCRKCEEACPQNT
                     IIALNFPPRKPKADDAAPQVKVAAPKAEVETPKAEV"
     protocluster    1..14578
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:4578]"
                     /cutoff="2000"
                     /detection_rule="(Complex1_51K and minimum(5, [FMN_bind,
                     FeS, Fer4, Fer4_9, NQR2_RnfD_RnfE, Rnf-Nqr, RnfC_N,
                     SLBB]))"
                     /neighbourhood="10000"
                     /product="Rnf_complex"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..4578
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="(Complex1_51K and minimum(5, [FMN_bind,
                     FeS, Fer4, Fer4_9, NQR2_RnfD_RnfE, Rnf-Nqr, RnfC_N,
                     SLBB]))"
                     /neighbourhood="10000"
                     /product="Rnf_complex"
                     /protocluster_number="1"
     cand_cluster    1..14578
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Complex1_51K and minimum(5, [FMN_bind,
                     FeS, Fer4, Fer4_9, NQR2_RnfD_RnfE, Rnf-Nqr, RnfC_N,
                     SLBB]))"
                     /kind="single"
                     /product="Rnf_complex"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..14578
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Rnf_complex"
                     /region_number="1"
                     /rules="(Complex1_51K and minimum(5, [FMN_bind, FeS, Fer4,
                     Fer4_9, NQR2_RnfD_RnfE, Rnf-Nqr, RnfC_N, SLBB]))"
                     /tool="gutsmash"
     CDS             307..1644
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: Complex1_51K"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: RnfC_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: SLBB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_10"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg33_2"
                     /sec_met_domain="Fer4_10 (E-value: 6.2e-09, bitscore: 27.3,
                     seeds: 59, tool: rule-based-clusters)"
                     /sec_met_domain="SLBB (E-value: 1.3e-15, bitscore: 48.5,
                     seeds: 67, tool: rule-based-clusters)"
                     /sec_met_domain="Complex1_51K (E-value: 3.3e-49, bitscore:
                     158.1, seeds: 88, tool: rule-based-clusters)"
                     /sec_met_domain="RnfC_N (E-value: 1.5e-29, bitscore: 93.4,
                     seeds: 53, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 0.00012, bitscore: 14.0,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLKTFSIGGVHPHENKLSAHQPIIKAEIPAKAAILLGQHIGAPAK
                     PIVAKGDVVKVGTKIAEPGGFVSAAIHSSVSGKVAKIDTIVDASGYAKPAIFIDVEGDE
                     WEESIDRSETLVKECSLTSEEIVKKIADAGIVGLGGACFPTQVKLCPPPAFKAECVIIN
                     AVECEPYLTADHQLMLEHAEEIMVGVSILMKAVKVNKAFIGIENNKPDAIQLMTKVASS
                     YAGIEVVPLKVQYPQGGEKQLIDAVISRQVTAGALPISTGAVVQNVGTAFAVYQAVQKN
                     KPLFERVITVTGKSLAKPSNFLARIGTPMKQLIEACGGLPEDTGKIIGGGPMMGKALVN
                     TDVPTAKGSSGILIMNEKEAKRGEVQPCIRCAKCVGACPMGLEPYLLATVSAHGDFERV
                     EKEDIMSCIECGSCQFTCPSNRPMLDYIRLGKGKVGAMIRARQAKK"
     CDS             1651..2643
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: NQR2_RnfD_RnfE"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg33_3"
                     /sec_met_domain="NQR2_RnfD_RnfE (E-value: 6.7e-119,
                     bitscore: 387.9, seeds: 472, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKLIVSLSPHVHGGDSVKKNMYGVLIALIPAFLVSLYFFGLGAL
                     IVTATSVAACLFFEWAIGKFLMKKETTTITDGSAIITGVLLAFNLPSNLPIWIIILGAL
                     FAIGVGKMSFGGLGCNPFNPALAGRVFLLLSFPVQMTTWPAVGQLTAYTDATTAATPLA
                     IMKGVINGAPGMSLSDLPGAFDLLIGNNGGCLGEVSALALLLGLFYMLWKKIITWHIPV
                     SILVTVFVFSGIMYLVNPELYVSPVVQLLSGGLMLGAVFMATDYVTSPMSHKGMLIYGV
                     CIGLLTVIIRLFGAYPEGMSFAILIMNAFTPLINTYVKPKRFGEVAKKK"
     CDS             2670..3305
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: FMN_bind"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg33_4"
                     /sec_met_domain="FMN_bind (E-value: 7.1e-24, bitscore:
                     75.7, seeds: 891, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLLVLTGVTAISVALLAYVNELTKEPIAQANAKTLSDAVSAVVPG
                     FDNDPIAEKKMQEVNGVQYSVYPATKGGEYIGAAVEAVAMGFGGELKVLVGFDAEGKII
                     DYSLLSHVETPGLGSKAADWFKKGNKGDITGMNPGEAALTVSKDGGKVDAITASTITSR
                     AFLNAVNAAYAAYAGQETADGTTGATQKNVEQPADSAAPEAVDTLSAK"
     CDS             3247..3906
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: Rnf-Nqr"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg33_5"
                     /sec_met_domain="Rnf-Nqr (E-value: 6e-71, bitscore: 229.4,
                     seeds: 650, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LNNLLIPQLPKLWIHSALNEKGVEDMNNFKVMMNGIIKENPTFVL
                     LLGMCPTLGTTSSAINGMGMGLATMFVLICSNVVISAIKNLIPDMVRIPSFIVVIASFV
                     TLLQMIMQAYVPALYATLGLFIPLIVVNCIVLGRAEAFAAKNNPVASLFDGLGMGLGFT
                     IALTLLGAVREFLGTGKIFNLSILPEEYGMLVFVLAPGAFIALGYLIALINSMKKN"
     CDS             3994..4578
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: Rnf-Nqr"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg33_6"
                     /sec_met_domain="Rnf-Nqr (E-value: 3.5e-64, bitscore:
                     207.3, seeds: 650, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIMEYILIFISAIFVNNIVLSQFLGICPFLGVSKKVETALGMSAA
                     VAFVLTIATIVTFLIQKYVLDAFDLGYLQTITFILVIAALVQMVEIILKKVSPSLYQAL
                     GVFLPLITTNCCILGVAILVIQKDYDLLTGVVYAFSTALGFGLALTLFAGLREQMSLVS
                     VPKGMQGTPIALITAGLLAMAFMGFSGVVKI"
     CDS             4709..5743
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GDP_Man_Dehyd"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg33_7"
                     /sec_met_domain="GDP_Man_Dehyd (E-value: 1.7e-53, bitscore:
                     173.5, seeds: 258, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKGKILVTGGTGYIGSHTVVELQNAGYEVVIIDNLSNSSADVVDN
                     IEKVSGIRPAFEELDCLDYAGLDAVFTKYKGIKAIIHFAASKAVGESVQKPLLYYRNNL
                     VSLINLLELMPKHGVEGIVFSSSCTVYGQPDELPVTEKAPIKKAESPYGNTKQINEEIV
                     RDTVASGAPINAILLRYFNPIGAHPTALLGELPNGVPQNLIPYLTQTAIGIREKLSVFG
                     DDYDTPDGSCIRDFINVVDLAKAHVVAIRRILENKQKEKVEVFNIGTGRGLSVLELINA
                     FEKATGVKLNYQIVGRRAGDIEKVWANPELANNELGWKAETSIEDTLLSAWKWQLKLRE
                     RGIQ"
     CDS             6354..7889
                     /locus_tag="ctg33_8"
                     /transl_table=11
                     /translation="MSQIVGHISQVIGPVVDVYFEGTEAEMMLPSIHDALEIKRSNGKR
                     LIVEVQQHIGENTVRTVAMDSTDGLQRGMTVHPLGGPITMPIGTQIKGRLMNVVGDSID
                     GMKELDRTGAYPIHRDPPKFEDLTTVQEVLYTGIKVIDLLEPYSKGGKIGLFGGAGVGK
                     TVLIQELINNIAKKQHGFSVFAGVGERTREGNDLLREMIESGVIRYGEEFKKGMEEGHW
                     DLSKVDYDEVAKSQVSLIFGQMNEPPGARQSVALSGLTVAESFRDMGAETDGPRDILFF
                     IDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGAMQERITSTRNGSITSVQAVYVP
                     ADDLTDPAPATTFTHLDATTVLSRKITELGIYPAVDPLESTSRILDPHIVGVEHYEVAQ
                     RVKQILQRNKELQDIISILGMEELSDADRTLVNRARRVQRFLSQPFAVAEQFTGVPGTM
                     VSIEDTIKGFKMILDGEVDYLPEPAFLNVGTIEEAIEKGKKLLEQAGGKTIKN"
     CDS             7906..8157
                     /locus_tag="ctg33_9"
                     /transl_table=11
                     /translation="MMKKLHLNIVSPEKELFNGEVESVTLPGTMGSFSILPQHAPIVSS
                     LGTGKLIYATDGEEHELDIQSGFVEMSNGRVAVCIEQQ"
     CDS             8199..8618
                     /locus_tag="ctg33_10"
                     /transl_table=11
                     /translation="VTITKRNYLLHTALLTVLIGIVGGWAYFSINPHHYFGGYPLIPLF
                     FFVFGVFMINMTESCRHRMPGRMLQIYLLMRVMRMLVSMIVMLVYCVAVREEARAFLLT
                     FIANYLIYLIYDSWFFFTFEANRKLKKKSNNEKIA"
     CDS             8602..9672
                     /locus_tag="ctg33_11"
                     /transl_table=11
                     /translation="MKRLRNILTVVLLALGMLLPATVRAENTVDVKEIVFGHIGDSYEW
                     HITTWGETHVTIPLPVIVHSSTTGWHAFLSSRLEENSGSYEGFSIAPAGSKYEGKLVEY
                     DATGNEIRPLDISITKVTLALLINSALLLLIILSVAHWYRKHPQGSAAPGGFIGFMEMF
                     IMMVNDDIIKSCVGPKYRKFAPYLLTAFFFIFINNIMGLIPFFPGGANVTGNIAITMVL
                     AICTFLAVNIFGTKTYWKDIFWPDVPWWLKVPVPMMPFIEFFGIFTKPFALMIRLFANM
                     LAGHMAMLVLTCLIFISASMGPALNGTLTVASVLFNIFMNALELLVAFIQAYVFTMLSA
                     VFIGLAQEEHKEKAVK"
     CDS             9729..9986
                     /locus_tag="ctg33_12"
                     /transl_table=11
                     /translation="MLLSVLLQAAAAGVGVSKLGAAIGAGLAVIGAGVGIGKIGGSAME
                     AIARQPEASGDIRMNMIIAAALIEGVALLAVVVCLLVFFL"
     CDS             10082..10588
                     /locus_tag="ctg33_13"
                     /transl_table=11
                     /translation="MSLLLPDSGLLFWMLLSFGVVFVVLAKYGFPVITKMVEGRKTYID
                     QSLEVAREANAQLSKLKEESEALIAAANKEQGRILREAMHERDKIIVEARKQAEAAAQK
                     ELDEVKKQIQQEKEEAIRDIRRQVAVLSVDIAEKIIRKNLDEKHEQMEMIDRMLDEVLA
                     ASKNN"
     CDS             10600..11160
                     /locus_tag="ctg33_14"
                     /transl_table=11
                     /translation="MDIGIVSMRYAKALMEYAKSMGAEDTLYKEFCMLDRSFRKHPDLR
                     MALENPILTIREKLTLICTAAVGDAPAGREFARFMTLVLKNRRENFLQYICLSFLDLYR
                     KDKHIGVGKLITAVPVSQEVRERIHDSASSLLHASMELQTEVDPSIEGGFIFDINDFRL
                     DASIATQLKKVKQQFIDKNRRIV"
     CDS             11291..12877
                     /locus_tag="ctg33_15"
                     /transl_table=11
                     /translation="MLSENIKVSEVSDILRKQLEGIDTRVQLDEIGTVLQVSDGVARIY
                     GLRNAEANELLEFDNGIKAIVMNLEEDNVGAVLLGPTDKIKEGFIVKRTKRIASIMVGE
                     GMLGRVIDPLGAPLDGKGLIGGELYEMPLERKAPGVIFRQPVNQPLQTGLKAVDAMIPI
                     GRGQRELIIGDRQTGKTAIAIDTIINQRANFEAGDPVYCIYVAIGQKGSTVASIVNTLH
                     QYGAMDYTIVVAATAGDPAALQYYAPFAGAAIGEYFRDTGRHALVVYDDLSKQAVAYRE
                     VSLILRRPSGREAYPGDIFYLHSRLLERAAKIISQEEVAREMNDLPDSLKGKVKGGGSL
                     TALPIIETQAGDVSAYIPTNVISITDGQIFLDTNLFNQGNRPAIDVGISVSRVGGNAQV
                     KAMKKVAGTLKIDQAQYRELEAFTKFSGDMDPVTALTIDKGQKNTRLLVQPQYSPMPVE
                     KQIAILYCGTHGLLRNVPLDKVSEFERNFLESLEMNYRMEVLDVLKTGAINDDVCKKIE
                     ETAEAVARQFM"
     CDS             12999..13889
                     /locus_tag="ctg33_16"
                     /transl_table=11
                     /translation="MSSLKEVKNRISSVKSTRQITSAMKMVASAKLHKAQGRIENMLPY
                     QRKLNEILTNFLRTDASFESPYTETRPVTRVAVVAFSSNSSLCGAFNSNVAKMLERTLE
                     SYQSLGKENILIYPVGRKVEEAVKKMGYVPQGSYQLMADKPSYVEAYELAEKLMHEFVE
                     KQIDHVELIYHHFKSMGSQVLLRENYLPIDLTQVAQEAAEDVPEDARSFNNDYIVEPSV
                     GELIAELLPKVLSQKIFTVLLDSNTSEHAARMLAMQTATDNANELIQDLTKQYNKSRQQ
                     AITNELLDIIGGSLK"
ORIGIN
        1 gcatgtatcg gttgcggcaa gtgtgtgaag gtttgtccgt tcgaggctat cacgctggaa
       61 aacaatctgg cttacattga ccccaataag tgcaaatcct gtcgcaagtg cgaggaagct
      121 tgtccgcaaa acacaatcat tgccctcaac ttcccgccgc gcaaaccgaa ggctgacgat
      181 gcagctcccc aagtaaaggt tgcagctcct aaagctgagg ttgagactcc caaagcagag
      241 gtttagactt cccaagttga ggtatgaatt aataaagtaa ataaacgact aaaaatacag
      301 aattgtatgt tgaagacatt ttcaattggt ggagtccatc cacacgaaaa taaactttca
      361 gcacatcagc ctatcatcaa ggcagagatt ccggccaagg ccgctatctt gctgggacag
      421 cacatcggtg ctcctgccaa gcctatcgtt gccaaaggtg atgttgtgaa agtgggtacg
      481 aagattgccg agcccggcgg ttttgtatca gcagccattc attcgtctgt cagcgggaaa
      541 gtcgctaaga ttgacacgat tgtcgatgcc agcggttacg ccaagcctgc tatattcatt
      601 gacgtagaag gggatgaatg ggaagaatcc attgaccgtt ccgaaacgtt ggtgaaggaa
      661 tgcagtctga cctccgagga aatcgtgaag aaaatcgctg atgccggtat cgtaggtctg
      721 ggcggtgcat gctttcctac gcaggtcaag ttgtgtcctc ctcctgcatt caaggcagag
      781 tgtgtcatca tcaacgccgt agagtgcgaa ccgtatctga ctgccgacca tcagctgatg
      841 ctggaacatg cggaagaaat tatggtaggt gtatccattc tgatgaaagc ggtaaaggtg
      901 aacaaggcat tcattggcat tgagaacaac aagcccgatg ccatccagct gatgacgaag
      961 gttgcttcca gctatgcagg tatcgaggtg gtacccttga aggtacaata tccgcagggt
     1021 ggtgaaaagc agttgattga tgctgtcatc agtcgccagg ttacagccgg tgcgctgcct
     1081 atctccacgg gagctgtggt gcagaatgtg ggtaccgcat ttgccgttta ccaggccgta
     1141 cagaagaaca aacctctgtt tgagcgtgtc attaccgtga ctggtaagtc gttggcaaag
     1201 ccgtccaatt tcctggcacg tatcggtacg ccgatgaagc agctgattga agcttgcggc
     1261 ggtctgcccg aagatacggg caagattatc ggcggtggcc cgatgatggg taaagctctg
     1321 gtaaatacgg atgtaccgac agccaaagga agctccggta ttctgattat gaacgagaag
     1381 gaagccaagc gcggcgaagt acagccttgt atccgttgtg ctaagtgcgt gggagcctgc
     1441 ccgatggggt tggagccgta tctgctggct accgtttcgg cccacggaga ttttgaaaga
     1501 gtagagaaag aagacattat gtcgtgtata gaatgcggtt catgccagtt cacttgtcct
     1561 tccaaccgtc cgatgctgga ttatatccgc ttgggtaagg gtaaggtagg agcgatgatc
     1621 cgtgcacgtc aagctaaaaa ataacgaagt atgaataaat taatcgtatc cctttcgccc
     1681 cacgttcacg gcggtgacag cgtgaagaag aatatgtacg gtgtgcttat cgcattgatt
     1741 ccggcattcc ttgtttcgct ttactttttc ggactgggtg cgctgattgt tacggccact
     1801 tccgtagcag cctgcttgtt ctttgaatgg gccatcggca aattcctcat gaaaaaagag
     1861 accacaacca ttaccgacgg ttctgccatc attaccggtg tgttgctggc ctttaacctg
     1921 ccttccaacc tgcccatctg gattatcatc cttggtgcct tgtttgccat cggggtaggc
     1981 aagatgtcct tcggaggttt gggttgcaat ccctttaacc ctgcactggc aggacgtgtg
     2041 ttcctgttgc tttcattccc cgtgcagatg acgacatggc cggctgtagg ccagttgaca
     2101 gcctatacgg atgccacgac agctgctacg ccgctggcta ttatgaaagg tgtcatcaat
     2161 ggtgctccgg gcatgtccct gagcgacctt ccgggagctt tcgaccttct gataggtaat
     2221 aatggaggat gtctcggtga ggtgagtgcc ttggcgctgc tgcttggttt gttctacatg
     2281 ttgtggaaga aaatcatcac ctggcacatt ccggtatcta ttctggtgac ggtattcgtg
     2341 ttctccggta tcatgtatct ggtcaatccg gaactttatg tatccccggt cgtacagttg
     2401 ttgtccggtg gtctgatgct gggtgctgtc tttatggcta cggactacgt gacttctccg
     2461 atgagccaca aaggtatgct gatttatggt gtttgcatcg gcctactgac tgtcatcatc
     2521 cgtctgttcg gtgcatatcc ggagggtatg tcgtttgcca ttctgattat gaatgcgttc
     2581 actccgctca tcaacaccta tgttaaacct aaacgctttg gggaggtagc gaagaagaaa
     2641 tgaaaaagtt agaatcatcc ttgaagaata tgttgctggt acttacgggc gttaccgcaa
     2701 tctccgtagc gttgctggct tatgtaaacg aattgacgaa ggagcccatt gcgcaggcca
     2761 atgcaaagac cttgagcgat gccgtaagtg cggtggttcc gggcttcgac aatgacccca
     2821 tcgccgagaa gaagatgcag gaagtaaacg gagtacagta ttctgtatat cctgccacca
     2881 aaggcggaga atatatcgga gctgctgtgg aagctgttgc catgggtttt ggcggtgagc
     2941 tgaaagtgtt ggtcggtttc gatgctgaag gcaaaatcat cgactactcc ttgttgtcgc
     3001 atgtggaaac tccggggttg ggttccaagg ctgccgactg gttcaagaaa ggcaataaag
     3061 gtgacatcac cggcatgaat ccgggtgaag ctgcattgac agtgagcaag gacggtggta
     3121 aagtagatgc cattacagcg tctaccatca cctcacgtgc ttttttgaat gcagtgaatg
     3181 cggcttatgc tgcctatgcc ggtcaggaaa ctgccgacgg cactaccggc gctactcaaa
     3241 agaatgttga acaacctgct gattccgcag ctcccgaagc tgtggataca ctcagcgcta
     3301 aatgagaaag gagtagaaga tatgaataat tttaaagtta tgatgaacgg gattatcaaa
     3361 gagaatccta cgtttgtact cctgctgggt atgtgtccta cactgggtac cacttcctct
     3421 gccattaacg gtatgggtat gggactggct acaatgttcg tgctgatatg ttccaatgtg
     3481 gtgatttctg ccatcaagaa tctgattccc gatatggtgc gtatcccttc attcattgta
     3541 gtcatagcat catttgtgac actgttgcag atgataatgc aagcttacgt tcctgcattg
     3601 tacgccacac tgggcctgtt cattccactg attgttgtaa actgtatcgt attgggacgt
     3661 gccgaggcat ttgctgccaa aaacaatccg gtggcttcct tgttcgacgg tctgggcatg
     3721 ggcttgggct ttaccattgc actgacatta ctgggtgccg tgcgtgagtt cctgggaacc
     3781 ggcaagatat tcaacttgag tatccttcct gaagaatacg gcatgttggt atttgtcctt
     3841 gctccgggtg cattcatcgc attgggctat ctcattgctt taatcaacag tatgaagaag
     3901 aattaataat gaaaaattaa aaatgaaaaa attgagaacg tatcggcggt aggctgccgg
     3961 gcttttaatt tttagttttt aattcttaat ttaatgatta tggaatatat attgatattt
     4021 atctcggcaa tctttgtaaa caacatcgta ttgtcgcaat tcttgggaat ttgcccgttc
     4081 ttgggagttt ccaagaaagt ggagactgca ttgggcatgt cggcagcggt ggcattcgtg
     4141 ctcaccatcg ccactatcgt gacattcctt attcagaagt atgtgctcga cgcttttgat
     4201 ttgggctatt tgcagactat tacctttatc ctggtgattg ccgcattggt gcagatggta
     4261 gagattatcc tgaagaaagt atctccttct ctgtatcagg ccttgggcgt gttcctgccg
     4321 ttgattacta ccaactgctg tattctcggt gtggctatcc ttgttatcca aaaagattat
     4381 gatttgctga cgggagtggt ttatgcattc tccacggcac ttggtttcgg cttggcactg
     4441 acactgtttg caggcctgcg cgagcaaatg agcctggtta gtgtaccgaa aggtatgcag
     4501 ggtactccga tagctttgat tactgccgga ctgctggcaa tggcattcat gggcttttcg
     4561 ggggttgtga agatttaaag aagaaaaacc ggaaataaag tggaaggggg ataaactaaa
     4621 atttatcccc ctttttctgt ttttattaat ttatttcctt acatttgtgg cgtattgata
     4681 acctaaaagc taaattatct atatttatat gaaagggaaa atcttagtta cgggtggaac
     4741 cggttatatt ggttctcaca cggtggtgga acttcaaaac gcaggttatg aagttgttat
     4801 tattgataat ttgtcaaact ccagcgcaga tgttgtagat aacatagaga aggtatcggg
     4861 catccgccct gcattcgagg aattggattg cttggattat gccggtcttg acgctgtgtt
     4921 taccaaatat aaaggcatta aagccatcat tcactttgcg gccagcaagg ctgtgggtga
     4981 atctgtccag aaaccattgc tttactatcg taataacctg gtttcactga taaacctgct
     5041 ggaattgatg ccgaagcatg gagtggaagg tatcgtattc tcttcttcct gcacagttta
     5101 cggccagccg gacgaacttc cggtaacgga aaaggcacct attaagaaag ctgagtctcc
     5161 gtatggaaac acgaagcaaa tcaatgaaga gattgtacgt gatacggttg cttccggtgc
     5221 tccgataaac gccatcttgt tgcgttactt caacccgata ggtgcacacc ccacagcatt
     5281 gctgggagag ttgcctaacg gtgtaccgca gaacttgatt ccttatctta cccagaccgc
     5341 tatcggcatc cgtgagaaac tgagcgtgtt tggtgacgat tatgatactc ccgacggttc
     5401 ttgtatccgc gacttcatca atgtagtcga tttggccaaa gcacacgtag tggctatccg
     5461 ccgtatcttg gagaacaagc agaaggaaaa agtggaagta ttcaatatcg gtacaggtcg
     5521 tggactttcc gtattggaac tgattaatgc tttcgaaaag gctaccggtg taaaactgaa
     5581 ctatcagata gtagggcggc gtgccggtga tattgagaag gtatgggcaa acccggaatt
     5641 ggcaaacaat gaactgggtt ggaaggcaga aacaagtatt gaagatactt tactttctgc
     5701 atggaaatgg cagttgaagc ttcgtgaaag aggtattcaa tgacaaactt tgaatttgtg
     5761 ttttttaaca aataggcaat aaacaaatgc ctccccgcat ttgtttataa tgtgcaaatc
     5821 agcctgaagc tgtttatgta tatgtgaata tccgtaacta gtacgtatgc ctccccggca
     5881 tagacaggta agattgcata gtagttttgt cgtgttttat tttgtgtttg tgttgtgaat
     5941 aagccttgtc gagagacaag gcttattttt tgtatattat atacagaacc tctgttgtca
     6001 attcagtgtt ttttcataag atgggtgggc aattcctttt tcttgctaat tgttaactga
     6061 cgttatcatg tctgtaacaa tttgttccaa ttgtagtatt aatcgttcaa aatagtcttt
     6121 tttgttcaaa aaatgctata cacggtaaca actacatgca aattgtttat acatttgcgt
     6181 tgttgtttat gtaactattg ggatgaacaa accatagctg acaaacgtta ataggtacga
     6241 gtacatagct gatgaaagac gcgacttggc tgacttgtta atgaggatac acgcgactta
     6301 caacttagta ttgggaaaaa taattaagga aatatagaat aaaaagattg cttatgtcac
     6361 agattgtcgg acatatctca caggtaatcg ggcctgtagt agacgtctat ttcgaaggaa
     6421 cggaggcaga aatgatgttg ccgagtattc acgacgcact ggaaataaaa aggtcgaacg
     6481 gcaaaagact gattgtagag gtacagcagc acattggtga aaacacggtg cgtactgttg
     6541 ctatggacag tacggacggc ctgcagagag gaatgacagt gcatccgctg ggaggcccca
     6601 tcaccatgcc gataggtaca caaatcaaag gccgcctgat gaacgtagtg ggcgactcta
     6661 tcgacggcat gaaggagttg gaccgtacag gtgcttatcc catccatcgc gaccccccta
     6721 agtttgaaga tttgactacc gtacaagagg tgctttatac aggcatcaag gttatcgact
     6781 tgctggaacc atatagcaag ggcggtaaaa tcggtctgtt tggtggcgcc ggtgtaggca
     6841 agacggtgct tatccaagag ctcattaata atattgcgaa gaagcagcac ggcttctctg
     6901 tgtttgccgg tgtaggagaa cgtacacgtg aaggcaacga cctgctgaga gaaatgattg
     6961 aatccggtgt tatccgttac ggagaagaat tcaagaaagg tatggaggaa ggccattggg
     7021 atttgtccaa agtagattat gacgaagtgg ccaaatcaca agtgtctttg atattcggtc
     7081 agatgaacga acctcccggt gcgcgtcagt ccgtggcatt gtctgggttg acggtggccg
     7141 agtccttccg tgacatggga gcggagacgg acggtccgcg tgacattctg ttcttcattg
     7201 ataatatctt ccgtttcacc caagccggtt ccgaggtttc ggccttgctg ggccgtatgc
     7261 cttctgccgt aggctatcag ccgacactgg caacggaaat gggtgccatg caggagcgta
     7321 ttacctctac aagaaacggc tccatcactt ccgtgcaggc tgtatatgtg cctgccgatg
     7381 acttgacgga cccggcacct gccacaacgt ttacccacct cgatgccacc acggtgttga
     7441 gtcgtaagat tactgaactt ggtatttatc ctgctgtgga cccgttggaa tctacttcac
     7501 gcattctcga cccacacatc gtgggggtag aacactacga agtggcacaa cgtgtaaaac
     7561 aaatattgca gcgcaacaag gaattgcaag acattatctc catccttggt atggaggaac
     7621 tctctgatgc cgaccgtacg ttggtgaacc gtgcacgtcg tgtgcagcgt ttcctttcac
     7681 agccgtttgc cgttgccgag cagtttacgg gtgtcccagg tacaatggtt tccattgagg
     7741 acactatcaa aggcttcaag atgattcttg acggagaggt ggactacctg cccgaacctg
     7801 ctttcttgaa tgtgggaacc attgaagaag ccattgagaa gggtaagaaa ctactggagc
     7861 aggcaggggg aaaaacgatt aaaaattaaa agtctgggag ctttgatgat gaaaaagttg
     7921 catttgaata ttgtttcacc ggagaaggaa ctcttcaacg gggaagtgga aagtgttaca
     7981 ttgccgggca ctatgggctc tttttctatc ctgccgcagc atgcaccgat tgtatcatcg
     8041 ttgggaacgg ggaaattgat ttacgcaacg gacggtgagg aacatgagtt ggatattcaa
     8101 agcggattcg tggagatgag taacggaagg gttgccgtct gtatagaaca gcaataggct
     8161 attaaaaaat taaaaattaa aaaatagagg ttgttggcgt gactattact aagagaaact
     8221 acttattgca tacagcgctc ttgacggtat tgataggaat tgtaggcggt tgggcgtatt
     8281 tttcgataaa tccgcatcac tatttcggcg gttatccgct gattccgctt ttcttctttg
     8341 ttttcggagt attcatgatt aatatgaccg aaagctgccg tcaccggatg ccgggacgaa
     8401 tgttgcagat ttatctgctg atgcgtgtga tgagaatgct ggtctccatg attgtgatgc
     8461 tggtttattg cgttgcagta cgtgaggaag ccagggcgtt tctgttgacg tttatagcca
     8521 actatctgat atatttgatt tatgattcct ggttcttctt cacttttgag gcgaaccgga
     8581 aactgaaaaa gaaaagtaac aatgaaaaga ttgcgtaaca tactgactgt agttctgctg
     8641 gcactgggca tgttgctacc cgccacggtg cgggccgaga atacggtgga cgtgaaggag
     8701 attgtcttcg ggcatatcgg cgattcttac gaatggcaca tcacgacgtg gggcgaaact
     8761 cacgttacga ttcctttgcc ggtaatagtg catagctcta ccaccggatg gcatgccttt
     8821 ctatcctcac gtctggagga gaacagtgga agttatgaag gcttttccat cgctcccgca
     8881 ggaagcaagt acgaaggcaa gctggtggag tatgatgcca ccggaaacga aatacgcccg
     8941 ttggacatct ccatcacgaa ggtgacactg gctttgctca tcaacagcgc attgctgctt
     9001 ctcatcattt tgagtgtggc gcactggtat cgcaagcacc cgcagggaag tgccgctccc
     9061 ggtggtttca tcgggtttat ggagatgttt atcatgatgg tgaacgatga tattataaag
     9121 agctgtgtcg gtccgaagta ccgtaaattc gcaccttact tgctgacggc cttcttcttt
     9181 attttcatca acaacattat ggggctgatt cctttctttc ccgggggggc caacgtgacg
     9241 ggaaatatag ccattacgat ggtattggcc atctgtactt tcttggccgt gaatatcttt
     9301 ggaaccaaga cttattggaa agatatcttt tggccggatg tgccctggtg gctgaaggta
     9361 ccggtcccca tgatgccgtt tatcgagttc ttcggtattt tcaccaaacc gtttgctttg
     9421 atgatacgtc tttttgccaa tatgctggca gggcacatgg caatgctggt gcttacctgc
     9481 ctgatattca tctcggccag tatgggacct gctttgaacg gaacgctgac cgtggcatcg
     9541 gtgttgttca atatctttat gaatgcactt gagttgttgg ttgcctttat ccaggcatat
     9601 gtgttcacca tgctgtcagc cgtgttcatc ggtctggcgc aggaagagca taaggagaag
     9661 gcggtgaagt gatatgatta ttgggaatat aaatattaat aaaaataacc atttaaaaac
     9721 tgaaagttat gttactatct gtattattgc aagctgctgc ggcaggtgtg ggagttagta
     9781 aactgggtgc agccattggt gctggtttgg ctgttattgg tgctggtgta ggtattggta
     9841 aaatcggcgg ttctgctatg gaggccattg cccgccagcc ggaagcatcc ggagatatcc
     9901 gtatgaacat gattattgcc gctgccttga ttgaaggtgt tgctttgttg gcagtagttg
     9961 tgtgtttatt ggtattcttt ttataatgta caatctacca tttaccattt acaatttgtc
    10021 cgtgcagcat caccgcgcag ccaaattgta aatcgtaaat tgtcaaatag taaataaaat
    10081 tatgtcattg ttattacccg atagtggtct gctgttctgg atgctccttt cattcggtgt
    10141 ggtgtttgtg gtgcttgcca agtacggttt tcccgttatc accaagatgg tggaaggtcg
    10201 caagacctac atcgaccagt ctttggaagt ggcacgcgaa gcaaatgccc agctctccaa
    10261 actgaaagag gagagtgaag cgttgattgc tgctgccaac aaagaacaag gacgtattct
    10321 gcgggaggcg atgcatgagc gtgacaagat tatcgtcgaa gcacgcaagc aggcggaagc
    10381 cgccgcgcag aaggagctgg acgaagttaa aaagcagata cagcaagaga aggaagaggc
    10441 catccgcgat attcgccgcc aggtggcagt cttgtccgta gatatagccg agaaaattat
    10501 ccgcaagaat ctggacgaga aacatgagca gatggagatg atagaccgga tgctggatga
    10561 agtgctggcc gcaagtaaaa acaattaatt ctcaaagaaa tggatatagg aatcgtttcg
    10621 atgcgttatg cgaaagcatt gatggaatat gccaagagca tgggggcaga agatactctg
    10681 tacaaggagt tttgtatgct cgaccgtagc ttcaggaaac atccggattt gcgcatggca
    10741 ttggaaaacc ccatcctgac gatacgtgag aagttaacac ttatctgtac cgctgctgtg
    10801 ggggatgctc ctgcgggtcg tgagtttgcg cgtttcatga ccctggtgtt gaagaaccgg
    10861 cgggaaaact ttctccagta catctgcctg agttttctgg atttgtaccg gaaagacaag
    10921 catatcggtg taggaaagct gataacagcc gttcccgtca gccaggaagt gcgggagcgc
    10981 atccatgaca gtgcttcctc cctgctgcat gccagcatgg agctgcagac tgaagtggat
    11041 ccctccattg aaggtggttt tatcttcgac atcaacgact tccgtctgga tgccagtatt
    11101 gctacgcagc ttaagaaagt gaaacagcag ttcattgata agaatagaag aatcgtgtga
    11161 tttacgattt gacaatttac aatttactta tttaatagtc ttgtgatgcg taagttgtag
    11221 aggtcaaaca gtatatgatt cgggttcttt caaatagtaa atagtaaatt gtaaaatagt
    11281 aaatactaag atgttgtctg aaaatataaa agtaagcgaa gtctccgata ttctccgcaa
    11341 gcaactggaa gggattgaca cccgcgtaca gttggacgag attggcacgg tactgcaagt
    11401 cagtgacggt gtagcccgta tctatgggtt gcgcaatgcc gaagccaacg agttgttgga
    11461 gttcgacaac ggcatcaagg ccattgtgat gaacctcgaa gaggataatg tgggtgctgt
    11521 gttgcttggc cctacggata aaatcaagga aggcttcatt gtaaagcgta ccaagcgtat
    11581 tgcttccatc atggtgggag agggtatgct gggccgtgta atagaccctc tgggtgcgcc
    11641 gctcgatggc aaaggactga taggcggaga actttatgaa atgccgctgg aacgcaaggc
    11701 gccgggagtt atcttccgtc agccggtgaa tcagccgcta cagaccggtc tgaaggctgt
    11761 ggatgccatg atacctattg gccgcggaca gcgtgaactg attatcggtg accgccagac
    11821 cggtaagacg gccattgcca tcgataccat catcaaccag cgtgccaatt ttgaagctgg
    11881 tgatccggtg tattgtattt atgtagccat tggtcagaaa ggttctaccg ttgcctctat
    11941 cgtgaatacc ctgcaccagt acggtgcgat ggactatacc attgtggtgg ctgctacggc
    12001 gggcgacccg gctgcattgc agtattacgc tccctttgca ggggctgcca tcggagagta
    12061 tttccgtgat accggccgtc atgcgctggt ggtctatgac gacttgtcca agcaggcggt
    12121 ggcctatcgt gaagtgtctc tgattctgcg ccgtccatca ggacgtgagg cctatccggg
    12181 tgatatcttc tatctgcact cccgtttgtt ggaacgtgcg gcgaagatta tcagccagga
    12241 ggaagtggca cgcgagatga acgacctgcc ggacagtctg aaaggcaagg tgaaaggtgg
    12301 cggttcgctg actgcattgc ccattatcga aactcaggca ggagacgtgt ccgcctatat
    12361 cccaacgaat gtgatttcaa tcactgacgg gcagatattc cttgatacga acctcttcaa
    12421 ccagggtaac cgtccggcca ttgacgtggg tatctctgtg agccgtgtgg gtggtaatgc
    12481 ccaagtcaag gctatgaaga aggtggcagg tacattaaag attgaccagg cacagtaccg
    12541 ggagctggaa gcgtttacca agttcagtgg cgatatggac cccgtgacgg cattgaccat
    12601 cgacaaggga cagaagaata cacgcctgct ggtacagccc caatacagcc cgatgccggt
    12661 agaaaagcag attgccattc tttattgcgg cacgcacggc ttgttgagga atgttccttt
    12721 ggacaaggtc agcgagtttg aacgcaactt ccttgagtct cttgagatga actatcgcat
    12781 ggaggtgctt gatgttttaa agacgggtgc catcaatgac gatgtctgca agaagattga
    12841 ggagacggca gaggcggttg caaggcaatt catgtaattt acgattggac gatttacgat
    12901 ttacgattgg ggctactcta tatggtaagt ggtaaatcgg ctgcaagcaa aagtaacttc
    12961 aatcgtaaat cgtaaataat taaatcgtaa atagatatat gtcttcactg aaagaggtaa
    13021 aaaatagaat aagctccgtc aagagtacac gccagataac atcggctatg aagatggtgg
    13081 catctgccaa actgcataag gcgcaggggc ggattgagaa tatgctgcct tatcaacgga
    13141 agctgaatga gattctgacg aactttctgc gtacggatgc ttccttcgag tcgccctata
    13201 ccgagacaag gcccgtgacg agggtagcgg tcgtggcgtt ttcgtccaat agttccttgt
    13261 gcggtgcatt caactcgaat gtggcgaaga tgctggaacg gactttggaa agttaccagt
    13321 cgctgggcaa ggagaatatt ctgatatatc ccgttgggag aaaggtggag gaagccgtca
    13381 agaaaatggg atatgtgccg caaggcagct atcagttgat ggcagacaag ccttcgtacg
    13441 ttgaagcgta tgagctggcg gagaagctga tgcatgagtt tgtggaaaag cagattgacc
    13501 atgtggagct gatttaccat cacttcaaat cgatgggttc gcaggtgctg ctccgcgaga
    13561 attatctgcc cattgacctg acgcaagtag cgcaggaggc ggcagaagat gtaccggagg
    13621 atgcccgcag ttttaataat gattacattg tggaaccttc cgtcggcgaa ctgatagcgg
    13681 aacttctgcc aaaagtgttg agtcagaaga tatttaccgt gcttctcgat tccaatactt
    13741 cggaacatgc tgcccgtatg cttgccatgc agacggcgac agataatgcc aacgagttga
    13801 ttcaagattt gacgaaacag tataacaaga gccgtcagca ggccattacg aatgagctgc
    13861 tcgatattat cggcggtagc ttgaaatagc ccggatcgcc ccgtaaaggg cttgttcagg
    13921 atgggtacaa ataaatggaa taataggaca aggataacga aataaccgct atctttgtcc
    13981 tattatcata tgtggctact gatgaaatcc gtatcattct gagagagagc ggattggttg
    14041 aataataaac atatcaatgg agaataatcc cgaaatagcg cttgcatggc aattcattga
    14101 aaacacaggc actcatcttt ttttgaccgg caaggcaggt accggtaaga caactttcct
    14161 gcgaaaactg agggtggaga gtccgaaacg gatgattgta cttgcaccga cgggaattgc
    14221 cgctatcaat gcaggcggtg tcaccatcca ctcctttttc caagtacctt ttgctcctta
    14281 tgtgcccgaa agctcattca gcgcagatgg caaggcaacc taccgttttc gttacgggaa
    14341 agagaaaatc aacattatac gcagcataga tttattggtc atcgatgaaa tcagtatggt
    14401 ccgtgccgac ttgctcgatt cggtggatgc cgtcttgagg cggttccgcg accggaacaa
    14461 accctttggc ggcgtgcaac tgctgatgat tggcgactta cagcagctgt ctcccgtagt
    14521 gaaagacgat gagtggcaga tgctgcgcaa atactatgac acgccctatt ttttctcc
//
