LOCUS       c00163_gnlPro..         4019 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|IHACFGHE_163.
ACCESSION   c00163_gnlPro..
VERSION     c00163_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 09:54:31
            Original ID  :: gnl|Prokka|IHACFGHE_163
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4019
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4019
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[754:3710]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      755..3710
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4019
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4019
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             3..686
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fn3-like"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3_C"
                     /gene_functions="other (smcogs) SMCOG1041:Fibronectin type
                     III-like domain (Score: 89.7; E-value: 3.4e-28)"
                     /gene_kind="other"
                     /locus_tag="ctg163_1"
                     /sec_met_domain="Fn3-like (E-value: 1.7e-29, bitscore:
                     89.7, seeds: 729, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3_C (E-value: 6.6e-30,
                     bitscore: 92.3, seeds: 116, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="ELAKTGKPVIALVMCGRPVIFNEIREHADAVLCTWWLGSEAGNAI
                     CNVLWGEYNPSAKLPMTFPAHVGQIPIYYQNKSTGRPTALQQTYKASYIDASTEPAYPF
                     GYGLSYTDFGYSDLKLLPGKDAGVHTEVSVKVTNTGKCAGEEVVQLYVQDKVASVTRPI
                     KELKGFRKIHLNAGESKTVAFQITDDALGFYDNEMNYMVEPGDFVFMVGGSSDDVQQIT
                     YNLKE"
     CDS             complement(755..1480)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 75.5;
                     E-value: 1.7e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg163_2"
                     /sec_met_domain="Radical_SAM (E-value: 5.5e-32, bitscore:
                     99.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCPFRCLYCANPDTIDVKGG
                     TPTTPEEILQMAISQKAFFGKKGGITFSGGEPTLQAEALIPLFKELKANGIHICLDSNG
                     GIWNEKVEELFSLTDLVLLDIKQFNPERHRTLTGRSNEQTLRTAAWLEEHEHPFWLRYV
                     LVPGYSDSEEDIRLLGESLGKYRQIQRVEILPYHRLGVHKYEVMGWDYKLKEVKENTPE
                     QLERAEKLFKEYFTNVITN"
     CDS             complement(1482..3710)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg163_3"
                     /sec_met_domain="Gly_radical (E-value: 1.7e-47, bitscore:
                     148.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1171.1, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKTFTNGLWNKEINVSDFVSKNIAPYTGDASFLQGPTERTKR
                     IWDICLKALEEERNNNGVRSLDNTTVSTITSHKAGYIDKDNELIVGLQTDELLKRSIKP
                     FGGINVVAKACRENGVEVDDKVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGIDRLIEAKQEDLRNLTGPMTEARIRLREEVAEQIKALKDIK
                     VMGEYYGLDLSHPATSAQEAVQWVYMAYLAAIKEQDGAAMSLGNVSSFLDIFIEYDLAH
                     GKINETFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTEAIGGRFNDGRVKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKDFCAKVSVDTSSIQYENDNLMREVRNC
                     DDYGIACCVSYQAIGKQIQFFGARANLAKALLLAINGGRCENTGTVMVKGIPVLTGETL
                     KFEEVMANYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMAFIDTNPRINLAYGVAGL
                     SIAIDSLSAIKYAKVTARRNEIGLTEGFDTEGSFPCFGNNDDRVDHLGVDLVYYFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGPTPEERVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLEDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
ORIGIN
        1 cggaactggc aaagactgga aaacctgtca ttgctcttgt catgtgtggg cgtccggtaa
       61 tcttcaatga gataagggaa catgctgatg cggtactgtg tacttggtgg ctggggagtg
      121 aggccggcaa tgccatatgc aatgtgttgt ggggagagta taatccttcg gctaaattgc
      181 ccatgacatt tcctgcacac gtagggcaga ttcccattta ttaccagaat aagagtaccg
      241 gtcgtcctac cgcattgcaa cagacttata aggcatccta tatcgatgct tccactgagc
      301 ctgcctatcc tttcggatat gggctttcgt atacggattt tggatattct gatttgaaac
      361 tgctgccggg caaggatgca ggcgtccata cggaggtgtc ggtaaaagtg actaataccg
      421 gtaagtgtgc aggagaggaa gtagtccagt tgtatgtgca ggataaggta gcgtctgtta
      481 cgcgcccgat aaaggagttg aagggcttca ggaaaattca tctgaatgcg ggtgaaagca
      541 aaactgtagc ttttcagata actgacgatg cactgggatt ctatgacaat gagatgaatt
      601 atatggtaga acctggtgat ttcgtcttta tggtgggtgg cagtagtgat gatgtacagc
      661 agattaccta taacttgaaa gaataggatt cttgtcctat aaatgaaaag caaggctgtc
      721 tggaatcata tccggacagc cttgcttttc tgttttagtt ggttatcaca ttcgtgaaat
      781 actctttgaa cagtttttct gcccgttcca gttgttccgg agtattttcc ttcacttctt
      841 tcaatttgta atcccatccc atgacttcgt atttgtgaac gcccagacgg tggtaaggaa
      901 gtatttccac ccgttgtatc tgccggtatt tgccgagact ttcacccagc aggcggatgt
      961 cttcttccga atcgctgtaa ccgggaacca gtacataacg cagccagaag gggtgttcat
     1021 gttcttcgag ccaggcagcg gtgcggagtg tctgttcgtt gcttcgtccc gtcagcgtac
     1081 ggtggcgttc gggattgaat tgcttgatgt ccagcagcac gaggtcagtc aggctaaaga
     1141 gctcttccac cttttcattc cagatgcctc cgttactgtc cagacagata tggatgccgt
     1201 tcgccttcag ctccttgaaa agaggaatca gcgcttctgc ttgcagggta ggctcaccgc
     1261 cggagaaagt aatacctcct ttcttcccga agaatgcctt ttggctgata gccatttgca
     1321 gaatctcttc cggagtggtg ggagtgcctc cttttacatc aatcgtatcc ggattggcac
     1381 aatacagaca acggaacgga catccttgca ggaaaacgac gagccggaga cccggcccgt
     1441 cgaatgttcc catactttcg taggagtgta catttatcat attacattct ttcgtggaat
     1501 gaacggctga tgacttccaa ctgatgctcg cggctcaact tcacgaagtt cacggcatag
     1561 ccggaaacgc ggatggtgag ttgcggatac ttttcaggat gttccatggc atcttccagc
     1621 atttcgcggt tcagtacgtt tacgttcaaa tggtgggcac ctttggtgaa gtagccgtcc
     1681 atcatggtta ccaggttctc tacacgttct tccggtgtcg ggcccagtga tttaggaaca
     1741 atggagaacg tattgctgat gccgtcctgc gagtcgcggt agcgcaattt ggctacggag
     1801 ctcaaggaag cgatggcacc gttcttgtca cgtccgtgca ttgggttggc acccggagca
     1861 aaggcaacac ctttggcgcg tccgtccgga gtggcaccgg tcttcttgcc gtacatcacg
     1921 ttggaggtga tagtcagcaa ggaaagggtg ggacgggcat tcttgtagac aggcagcttt
     1981 ttcagttctt cgctgaaata gtaaacgagg tctactccca ggtggtccac acggtcgtca
     2041 ttattgccga agcaggggaa tgagccttca gtgtcgaaac cttcggtcag gccgatttcg
     2101 ttgcggcgtg cagtgacttt ggcatatttg atggcggaca gtgagtctat ggcgatggaa
     2161 aggccggcaa caccgtatgc cagattgatg cgtgggttgg tatcgatgaa tgccatctgg
     2221 gccttctcat aataatactt atcatgcatg tagtggatga tattcatggc ctcgttgtag
     2281 acacgggcta tttcagtcag taccttcttg tagttggcca taacttcctc aaacttcagt
     2341 gtctcgccgg tcaatacggg aatacctttc accatgacag ttccggtgtt ttcgcaacgg
     2401 ccaccgttga tggcaagcag cagggccttg gcaaggttgg cacgtgcgcc gaagaactgg
     2461 atttgcttgc cgatggcttg gtaggataca cagcaggcaa tgccgtaatc gtcgcagttg
     2521 cgcacttcgc gcatcaggtt gtcgttttcg tactggatgg agctggtatc tacggaaact
     2581 ttggcgcaga aatccttgaa gccttccggc agttcgggac tccacagtac ggtcaagttt
     2641 ggttccggag aaggaccgag gttgtatagc gtctgcaaga agcggaaaga agtcttggtg
     2701 actttcacgc gtccgtcatt gaagcgtcct ccgatggctt cggtcaccca agtcgggtca
     2761 ccggcaaaga tatcattgta cgactgcata cgcagatggc gtaccatgcg cagcttgatg
     2821 acgaactggt ctatcagttc ttgcgcaaac gtctcattga tttttccgtg agccagatcg
     2881 tattcaatga atatatcgag gaacgaagat acgttaccca atgacatggc agcaccgtct
     2941 tgctctttga tggcagcaag atatgccatg tatacccatt gcacggcctc ttgagctgat
     3001 gtagccggat ggctgaggtc gagtccgtaa tattcgccca taaccttgat gtccttcaaa
     3061 gctttgattt gttccgccac ttcttcgcgt agacggatgc gtgcttcggt catcgggccg
     3121 gtgaggttgc gcaagtcctc ctgcttggct tcaatcagac ggtcgatgcc gtagagagcc
     3181 aatcggcggt agtcgccgat gatacgtccg cgggcatagt tgtcgggcaa tccggtaagg
     3241 aatcccagtg agcggaagga acggatttct tcggtatata catcgaatac gccgtcgtta
     3301 tgtgttttgc ggtaatgggt gaagatgtct tttactttat cgtccacttc cactccgttt
     3361 tcacggcatg cttttgctac cacgttgatg cctccgaaag gtttgatgga tcgtttcaac
     3421 agttcatcag tctgcaaacc gacaataagt tcattgtcct tatcaatata cccggcttta
     3481 tgagaagtga tggtagatac ggtggtattg tccagtgaac ggacgccatt gttgttcctt
     3541 tcttcttcga gagccttcag acagatgtcc cagatacgtt tggtacgttc ggtaggtccc
     3601 tgcaagaaag atgcatcccc ggtatagggt gcgatgtttt tactgacgaa atcactgaca
     3661 ttgatttctt tattccaaag cccattggta aaggttttgt tcaattccat aaaatttgtt
     3721 tttgttagta ggttagataa tgttatcctt tagagcgcac aaaaatactg aataaattcc
     3781 gtaattttat aagagatact tctcttttta tgttgaacag cataaaataa ctattattag
     3841 gtagtgaatg tccgggggag tagagatgtt aatactttct atatgtggtt tatgaattga
     3901 aacaagaacc gaataataat ctgcattatt tacaaatgat gtaggtttaa ggaacagtcg
     3961 tattctgaac ctaattcagt cgtatagtga atgccattca gtcgtatact gaatagggt
//
