LOCUS       c00850_gnlPro..         1405 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|ILCJIMHF_850.
ACCESSION   c00850_gnlPro..
VERSION     c00850_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 14:26:45
            Original ID  :: gnl|Prokka|ILCJIMHF_850
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 1405
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..1405
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:1405]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2..1405
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..1405
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..1405
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(2..466)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.2;
                     E-value: 1.7e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg850_1"
                     /sec_met_domain="Radical_SAM (E-value: 8.3e-28, bitscore:
                     85.0, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GEVKYQMASDELLVEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKALEVLDYVDLVLLDIKTLNPDLHLLLTGVKQDHTLLFLEELE"
     CDS             complement(467..1405)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg850_2"
                     /sec_met_domain="Gly_radical (E-value: 1.7e-47, bitscore:
                     147.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 8.4e-68, bitscore:
                     216.3, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="YAINGGRDERSGVQVAPMFEPVRGEYLEYDEVMAKYEQMMRWLAK
                     VYVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGIAGLSIVADSLAAIRDTKVRVI
                     RDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFMEYLRQHETYRHAKPTQSILTI
                     TSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGAIAALASVAKLPFQHAHDGISY
                     TFAVSPATLGKEREVQINNLVSLLDGYFTPDGGQHLNVNVFDKDLLIDAMEHPEKYPQL
                     TIRVSGYAVNFVKLTREQQLDVISRTINHSL"
ORIGIN
        1 tctccaattc ttccaagaac agaagcgtat gatcctgctt gacacccgtc agcaacaaat
       61 gtaaatccgg gtttaaggtt ttgatatcca acaatactaa atccacataa tctaaaactt
      121 ccaaagcttt tgaagtacat acgaaaccgg atgtatccaa ggccgtgtga agaccttgct
      181 cttggcacaa acggaaaaac tctttcaaga actccggttg caataaaggt tctccaccgg
      241 taacagtcac tcccccacgg gcgataaaac ttttataccg taagacttcc accaataact
      301 cgtcggaagc catctgatat ttcacctccc ctttcggatt ccaagtatcc gggttatggc
      361 aatacaggca acgcaacggg caaccctgca taaaaacgac aaagcgaatt ccgggaccat
      421 ctacggtccc gaaactctct aatgaatgta tcttaccttt tatcacttac aatgaatggt
      481 ttatggttct tgaaataaca tccaactgtt gttcacgagt cagtttcacg aagtttaccg
      541 catatccgga tacacggatc gtaagttgcg gatatttctc aggatgttcc atagcgtcga
      601 tcaacaaatc cttatcaaat acgttaacat tcagatgctg acctccatcc ggagtaaaat
      661 agccatccaa taagctaacc agattattga tctgcacttc acgctccttt cccaacgtag
      721 ccggagaaac agcgaaagta taagagatac cgtcgtgcgc atgctggaat ggaagcttcg
      781 ctaccgaagc tagcgcagcg atagcacctt tcgtatcacg accattcatc gggttcgcgc
      841 ccggagcgaa aggagttcca ccgatacggc catccggtgt cgtaccagtc ttcttaccat
      901 ataccacatt agaggtgatc gtaaggatgg actgcgtagg tttcgcatgg cgataggtct
      961 cgtgctgacg caaatattcc atgaacttct cggtgatctc tacagcgata ctatccgtac
     1021 gatcatcgtt attaccgaaa ggaacatatt cgccctcacg ttcgaaatca accgccaagc
     1081 cacgctcgtc acggatcaca cgtactttcg tatcacggat agcagccaac gaatcggcaa
     1141 cgatagaaag accggcgata cctgtcgcac ggatacgctc tacatcccca tcgtgaagtg
     1201 acatctcaaa tgcctcatac gcatatttat cgtgcatata atggataatc ttcaaagcgt
     1261 tcacatatac cttcgccaac caacgcatca tttgctcata cttagccatc acctcatcgt
     1321 actccagata ctcaccgcga accggctcga acatcggggc tacctgtacg ccgctacgct
     1381 catcccgtcc tccgttgatc gcata
//
